{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T18:17:10Z","timestamp":1783189030654,"version":"3.54.6"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82573221"],"award-info":[{"award-number":["82573221"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018573","name":"Sun Yat-sen University Cancer Center","doi-asserted-by":"publisher","award":["YTP-SYSUCC-0045"],"award-info":[{"award-number":["YTP-SYSUCC-0045"]}],"id":[{"id":"10.13039\/501100018573","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.bbrc.2026.153918","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T02:19:00Z","timestamp":1778552340000},"page":"153918","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A click-compatible BmTyr platform for biotin-free proximity labeling and subcellular proteome profiling in primary T cells"],"prefix":"10.1016","volume":"822","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4679-1939","authenticated-orcid":false,"given":"Xin-Nan","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Min","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Ying","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Suo","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3640-2290","authenticated-orcid":false,"given":"Hao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3661-4453","authenticated-orcid":false,"given":"Lin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2026.153918_bib1","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/35015694","article-title":"Protein function in the post-genomic era","volume":"405","author":"Eisenberg","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2026.153918_bib2","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/s41576-022-00468-7","article-title":"Histone post-translational modifications - cause and consequence of genome function","volume":"23","author":"Millan-Zambrano","year":"2022","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.bbrc.2026.153918_bib3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.cell.2015.09.053","article-title":"A human interactome in three quantitative dimensions organized by stoichiometries and abundances","volume":"163","author":"Hein","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib4","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.cell.2015.06.043","article-title":"The BioPlex network: a systematic exploration of the human interactome","volume":"162","author":"Huttlin","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib5","doi-asserted-by":"crossref","DOI":"10.1002\/cpps.70","article-title":"Yeast two-hybrid assay to identify interacting proteins","volume":"95","author":"Paiano","year":"2019","journal-title":"Curr. Protoc. Protein Sci."},{"key":"10.1016\/j.bbrc.2026.153918_bib6","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ab.2005.01.018","article-title":"Proximity ligation assays for sensitive and specific protein analyses","volume":"345","author":"Gustafsdottir","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bbrc.2026.153918_bib7","article-title":"Protein-protein interactions identified by pull-down experiments and mass spectrometry","volume":"17","author":"Brymora","year":"2004","journal-title":"Curr. Protoc. Cell Biol. Chapter"},{"key":"10.1016\/j.bbrc.2026.153918_bib8","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1016\/j.cell.2024.10.038","article-title":"Discovery and significance of protein-protein interactions in health and disease","volume":"187","author":"Greenblatt","year":"2024","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib9","doi-asserted-by":"crossref","first-page":"320","DOI":"10.3390\/biology3020320","article-title":"On-beads digestion in conjunction with data-dependent mass spectrometry: a shortcut to quantitative and dynamic interaction proteomics","volume":"3","author":"Turriziani","year":"2014","journal-title":"Biology"},{"key":"10.1016\/j.bbrc.2026.153918_bib10","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/978-1-0716-3362-5_15","article-title":"Immunoprecipitation: variations, considerations, and applications","volume":"2699","author":"Noone","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib11","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/prot.26642","article-title":"Biotin protein ligase as you like it: either extraordinarily specific or promiscuous protein biotinylation","volume":"92","author":"Cronan","year":"2024","journal-title":"Proteins"},{"key":"10.1016\/j.bbrc.2026.153918_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2022.100256","article-title":"MicroID2: a novel biotin ligase enables rapid proximity-dependent proteomics","volume":"21","author":"Johnson","year":"2022","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.bbrc.2026.153918_bib13","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nbt.4201","article-title":"Efficient proximity labeling in living cells and organisms with TurboID","volume":"36","author":"Branon","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2026.153918_bib14","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s42003-022-03604-5","article-title":"Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells","volume":"5","author":"Kubitz","year":"2022","journal-title":"Commun. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib15","doi-asserted-by":"crossref","first-page":"13123","DOI":"10.1021\/jacs.6b06828","article-title":"Direct proximity tagging of small molecule protein targets using an engineered NEDD8 ligase","volume":"138","author":"Hill","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbrc.2026.153918_bib16","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/s41592-018-0100-5","article-title":"A proximity-tagging system to identify membrane protein-protein interactions","volume":"15","author":"Liu","year":"2018","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbrc.2026.153918_bib17","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nmeth.3179","article-title":"Directed evolution of APEX2 for electron microscopy and proximity labeling","volume":"12","author":"Lam","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbrc.2026.153918_bib18","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1110\/ps.04911804","article-title":"Promiscuous protein biotinylation by Escherichia coli biotin protein ligase","volume":"13","author":"Choi-Rhee","year":"2004","journal-title":"Protein Sci."},{"key":"10.1016\/j.bbrc.2026.153918_bib19","doi-asserted-by":"crossref","first-page":"12143","DOI":"10.1073\/pnas.1919528117","article-title":"Split-TurboID enables contact-dependent proximity labeling in cells","volume":"117","author":"Cho","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2026.153918_bib20","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41592-023-01880-5","article-title":"Engineered allostery in light-regulated LOV-turbo enables precise spatiotemporal control of proximity labeling in living cells","volume":"20","author":"Lee","year":"2023","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbrc.2026.153918_bib21","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1016\/j.cell.2023.05.044","article-title":"Dynamic mapping of proteome trafficking within and between living cells by TransitID","volume":"186","author":"Qin","year":"2023","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib22","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1126\/science.1230593","article-title":"Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging","volume":"339","author":"Rhee","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2026.153918_bib23","doi-asserted-by":"crossref","first-page":"3885","DOI":"10.1021\/jacs.8b10845","article-title":"Enzymatic modification of N-Terminal proline residues using phenol derivatives","volume":"141","author":"Maza","year":"2019","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbrc.2026.153918_bib24","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/nbt.2375","article-title":"Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy","volume":"30","author":"Martell","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2026.153918_bib25","doi-asserted-by":"crossref","first-page":"e197","DOI":"10.1016\/j.jid.2017.09.028","article-title":"Research techniques made simple: emerging methods to elucidate protein interactions through spatial proximity","volume":"137","author":"Che","year":"2017","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.bbrc.2026.153918_bib26","doi-asserted-by":"crossref","first-page":"7515","DOI":"10.1021\/jacs.3c13183","article-title":"Tyrosinase-based proximity labeling in living cells and in vivo","volume":"146","author":"Zhu","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbrc.2026.153918_bib27","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1038\/s41467-025-57767-w","article-title":"Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID","volume":"16","author":"Zhang","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2026.153918_bib28","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.chembiol.2020.05.006","article-title":"A clickable APEX probe for proximity-dependent proteomic profiling in yeast","volume":"27","author":"Li","year":"2020","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib29","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1038\/ni.2393","article-title":"Mouse hobit is a homolog of the transcriptional repressor Blimp-1 that regulates NKT cell effector differentiation","volume":"13","author":"van Gisbergen","year":"2012","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.bbrc.2026.153918_bib30","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1126\/science.aad2035","article-title":"Hobit and Blimp1 instruct a universal transcriptional program of tissue residency in lymphocytes","volume":"352","author":"Mackay","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2026.153918_bib31","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1016\/j.bbrc.2017.09.168","article-title":"A simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat","volume":"493","author":"Cheah","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2026.153918_bib32","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/978-1-0716-2473-9_10","article-title":"The NanoBiT-Based homogenous ligand-receptor binding assay","volume":"2525","author":"Liu","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib33","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf496","article-title":"A flexible, high-throughput system for studying live mRNA translation with HiBiT technology","volume":"53","author":"Ascanelli","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbrc.2026.153918_bib34","doi-asserted-by":"crossref","first-page":"6895","DOI":"10.1038\/s41598-019-43319-y","article-title":"HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions","volume":"9","author":"Ranawakage","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2026.153918_bib35","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1021\/bc060195l","article-title":"Highly-efficient purification of native polyhistidine-tagged proteins by multivalent NTA-Modified magnetic nanoparticles","volume":"18","author":"Kim","year":"2007","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.bbrc.2026.153918_bib36","doi-asserted-by":"crossref","first-page":"19955","DOI":"10.1021\/acs.jmedchem.5c00459","article-title":"Comparative profiling and chemogenomics application of chemical tools for NR4A nuclear receptors","volume":"68","author":"Willems","year":"2025","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbrc.2026.153918_bib37","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1038\/s41564-025-02140-2","article-title":"Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages","volume":"10","author":"Cheng","year":"2025","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.bbrc.2026.153918_bib38","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41551-023-01073-7","article-title":"Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS-STING pathway","volume":"8","author":"An","year":"2024","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.bbrc.2026.153918_bib39","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.smim.2014.05.004","article-title":"Regulation of NF-kappaB by TNF family cytokines","volume":"26","author":"Hayden","year":"2014","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.bbrc.2026.153918_bib40","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.it.2020.10.013","article-title":"NF-kappaB and extrinsic cell death pathways - entwined do-or-die decisions for T cells","volume":"42","author":"Blanchett","year":"2021","journal-title":"Trends Immunol."},{"key":"10.1016\/j.bbrc.2026.153918_bib41","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a000158","article-title":"The IKK complex, a central regulator of NF-kappaB activation","volume":"2","author":"Israel","year":"2010","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib42","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/j.molcel.2014.01.026","article-title":"Fold change of nuclear NF-kappaB determines TNF-induced transcription in single cells","volume":"53","author":"Lee","year":"2014","journal-title":"Mol. Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib43","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.bbrc.2003.09.074","article-title":"Identification of a nuclear protein that promotes NF-kappaB activation","volume":"310","author":"Chen","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2026.153918_bib44","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1038\/s41418-023-01182-5","article-title":"NKAP acts with HDAC3 to prevent R-loop associated genome instability","volume":"30","author":"Zhang","year":"2023","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.bbrc.2026.153918_bib45","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.4049\/jimmunol.1800862","article-title":"NKAP must associate with HDAC3 to regulate hematopoietic stem cell maintenance and survival","volume":"202","author":"Shapiro","year":"2019","journal-title":"J. Immunol."},{"key":"10.1016\/j.bbrc.2026.153918_bib46","first-page":"5","article-title":"Copper homeostasis and cuproptosis in health and disease","author":"Yang","year":"2024","journal-title":"MedComm"},{"key":"10.1016\/j.bbrc.2026.153918_bib47","doi-asserted-by":"crossref","first-page":"15711","DOI":"10.1021\/jacs.0c02547","article-title":"Imaging and profiling of proteins under oxidative conditions in cells and tissues by hydrogen-peroxide-responsive labeling","volume":"142","author":"Zhu","year":"2020","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbrc.2026.153918_bib48","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1021\/acscentsci.4c00373","article-title":"Proximitomics by reactive species","volume":"10","author":"Zhang","year":"2024","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.bbrc.2026.153918_bib49","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.cell.2017.03.028","article-title":"Multidimensional tracking of GPCR signaling via peroxidase-catalyzed proximity labeling","volume":"169","author":"Paek","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2026.153918_bib50","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.chembiol.2024.03.012","article-title":"Current advances in photocatalytic proximity labeling","volume":"31","author":"Knutson","year":"2024","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib51","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1038\/ncb3586","article-title":"Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence","volume":"19","author":"Gluck","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbrc.2026.153918_bib52","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1038\/ni.3558","article-title":"Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing","volume":"17","author":"Chen","year":"2016","journal-title":"Nat. Immunol."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26006820?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26006820?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T17:43:57Z","timestamp":1783187037000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X26006820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":52,"alternative-id":["S0006291X26006820"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153918","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A click-compatible BmTyr platform for biotin-free proximity labeling and subcellular proteome profiling in primary T cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153918","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"153918"}}