{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:36:53Z","timestamp":1786628213508,"version":"build-2736575974"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFB3210300"],"award-info":[{"award-number":["2023YFB3210300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172077"],"award-info":[{"award-number":["82172077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22201298"],"award-info":[{"award-number":["22201298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62027825"],"award-info":[{"award-number":["62027825"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005085","name":"Beijing Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008235","name":"Peking Union Medical College Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008235","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.bbrc.2026.154099","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:29:34Z","timestamp":1781540974000},"page":"154099","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Visualization and detection of ciliary beating frequency in human airway organoids based on image correlated methods for drug screening"],"prefix":"10.1016","volume":"829","author":[{"given":"Ruimeng","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoyun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxing","family":"Bing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naixin","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1319-3166","authenticated-orcid":false,"given":"Wenfei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2026.154099_bib1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.semcdb.2020.11.007","article-title":"Motile cilia and airway disease","volume":"110","author":"Legendre","year":"2021","journal-title":"Semin. Cell Dev. Biol."},{"issue":"1","key":"10.1016\/j.bbrc.2026.154099_bib2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/s42005-025-02030-3","article-title":"Cilia beating misalignment, immotility or death pathologically affect the efficiency of mucus-clearance","volume":"8","author":"Laborie","year":"2025","journal-title":"Commun. Phys."},{"issue":"2","key":"10.1016\/j.bbrc.2026.154099_bib3","doi-asserted-by":"crossref","first-page":"P3","DOI":"10.1164\/rccm.1982P3","article-title":"Primary Ciliary Dyskinesia (PCD)","volume":"198","author":"Shapiro","year":"2018","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"4","key":"10.1016\/j.bbrc.2026.154099_bib4","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1002\/alr.23254","article-title":"Primary ciliary dyskinesia: an update on contemporary diagnosis","volume":"14","author":"Chaskes","year":"2024","journal-title":"Int. Forum Allergy Rhinol."},{"issue":"4","key":"10.1016\/j.bbrc.2026.154099_bib5","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.00725-2019","article-title":"International consensus guideline for reporting transmission electron microscopy results in the diagnosis of primary ciliary dyskinesia (BEAT PCD TEM Criteria)","volume":"55","author":"Shoemark","year":"2020","journal-title":"Eur. Respir. J."},{"issue":"6","key":"10.1016\/j.bbrc.2026.154099_bib6","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1164\/rccm.201510-2067RR","article-title":"Primary ciliary dyskinesia: ciliary beat pattern\/frequency, role of molecular analysis in the diagnosis, and neonatal distress","volume":"193","author":"Dy","year":"2016","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"10","key":"10.1016\/j.bbrc.2026.154099_bib7","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1513\/AnnalsATS.202110-1175OC","article-title":"High nasal nitric oxide, cilia analyses, and genotypes in a retrospective cohort of children with primary ciliary Dyskinesia","volume":"19","author":"Legendre","year":"2022","journal-title":"Ann. Am. Thorac. Soc."},{"issue":"2","key":"10.1016\/j.bbrc.2026.154099_bib8","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.tibtech.2019.06.001","article-title":"Bringing microscopy-by-sequencing into view","volume":"38","author":"Boulgakov","year":"2020","journal-title":"Trends Biotechnol."},{"issue":"1","key":"10.1016\/j.bbrc.2026.154099_bib9","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.02300-2021","article-title":"Methods for the assessment of human airway ciliary function","volume":"60","author":"Jackson","year":"2022","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.bbrc.2026.154099_bib10","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1016\/j.csbj.2023.02.036","article-title":"Light-sheet laser speckle imaging for cilia motility assessment","volume":"21","author":"Long","year":"2023","journal-title":"Comput. Struct. Biotechnol. J."},{"issue":"6","key":"10.1016\/j.bbrc.2026.154099_bib11","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1242\/dev.140103","article-title":"Lung organoids: current uses and future promise","volume":"144","author":"Barkauskas","year":"2017","journal-title":"Development"},{"issue":"3","key":"10.1016\/j.bbrc.2026.154099_bib12","first-page":"153","article-title":"PDX models for functional precision oncology and discovery science","volume":"25","author":"Blanchard","year":"2025","journal-title":"Nat. Rev. Cancer"},{"issue":"6","key":"10.1016\/j.bbrc.2026.154099_bib13","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1056\/NEJMra1806175","article-title":"Organoids - preclinical models of human disease","volume":"380","author":"Li","year":"2019","journal-title":"N. Engl. J. Med."},{"issue":"8","key":"10.1016\/j.bbrc.2026.154099_bib14","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1111\/j.1476-5381.2012.01929.x","article-title":"Roflumilast N-oxide, a PDE4 inhibitor, improves cilia motility and ciliated human bronchial epithelial cells compromised by cigarette smoke in vitro","volume":"166","author":"Milara","year":"2012","journal-title":"Br. J. Pharmacol."},{"issue":"11","key":"10.1016\/j.bbrc.2026.154099_bib15","article-title":"Long-term macrolides in chronic respiratory diseases: dusk or a new dawn? A narrative review","volume":"14","author":"Previtero","year":"2025","journal-title":"Antibiotics (Basel)"},{"issue":"3\u20134","key":"10.1016\/j.bbrc.2026.154099_bib16","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1159\/000338505","article-title":"Procaterol-stimulated increases in ciliary bend amplitude and ciliary beat frequency in mouse bronchioles","volume":"29","author":"Komatani-Tamiya","year":"2012","journal-title":"Cell. Physiol. Biochem."},{"issue":"23","key":"10.1016\/j.bbrc.2026.154099_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242316976","article-title":"Ambroxol-enhanced frequency and amplitude of beating cilia controlled by a voltage-gated Ca(2+) channel, Cav1.2, via pH(i) increase and [Cl(-)](i) decrease in the lung airway epithelial cells of mice","volume":"24","author":"Nakahari","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2026.154099_bib18","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1146\/annurev-physiol-021014-071931","article-title":"Cilia dysfunction in lung disease","volume":"77","author":"Tilley","year":"2015","journal-title":"Annu. Rev. Physiol."},{"issue":"6","key":"10.1016\/j.bbrc.2026.154099_bib19","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.01126-2025","article-title":"European respiratory society clinical practice guideline for the management of adult bronchiectasis","volume":"66","author":"Chalmers","year":"2025","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.bbrc.2026.154099_bib20","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2021.767621","article-title":"Human organoids for predictive toxicology research and drug development","volume":"12","author":"Matsui","year":"2021","journal-title":"Front. Genet."},{"issue":"1","key":"10.1016\/j.bbrc.2026.154099_bib21","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s13045-018-0662-9","article-title":"Organoid technology and applications in cancer research","volume":"11","author":"Xu","year":"2018","journal-title":"J. Hematol. Oncol."},{"issue":"1","key":"10.1016\/j.bbrc.2026.154099_bib22","article-title":"Identification of nicotinic acetylcholine receptor for N-Acetylcysteine to rescue nicotine-induced injury using beating cilia in primary tissue derived airway organoids","volume":"12","author":"Zheng","year":"2025","journal-title":"Adv. Sci. (Weinh.)"},{"issue":"5","key":"10.1016\/j.bbrc.2026.154099_bib23","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1165\/rcmb.2013-0363OC","article-title":"FOXJ1 prevents cilia growth inhibition by cigarette smoke in human airway epithelium in vitro","volume":"51","author":"Brekman","year":"2014","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"8","key":"10.1016\/j.bbrc.2026.154099_bib24","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1016\/j.devcel.2021.03.006","article-title":"RNA demethylation by FTO stabilizes the FOXJ1 mRNA for proper motile ciliogenesis","volume":"56","author":"Kim","year":"2021","journal-title":"Dev. Cell"},{"issue":"5","key":"10.1016\/j.bbrc.2026.154099_bib25","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/jmcb\/mjy043","article-title":"Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat","volume":"11","author":"Zhang","year":"2019","journal-title":"J. Mol. Cell Biol."},{"issue":"2","key":"10.1016\/j.bbrc.2026.154099_bib26","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.01769-2023","article-title":"Analyses of 1236 genotyped primary ciliary dyskinesia individuals identify regional clusters of distinct DNA variants and significant genotype-phenotype correlations","volume":"64","author":"Raidt","year":"2024","journal-title":"Eur. Respir. J."},{"issue":"6","key":"10.1016\/j.bbrc.2026.154099_bib27","article-title":"The miR-669a-5p\/G3BP\/HDAC6\/AKAP12 axis regulates primary cilia length","volume":"11","author":"Wang","year":"2024","journal-title":"Adv. Sci. (Weinh.)"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26008636?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26008636?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:00:52Z","timestamp":1786626052000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X26008636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":27,"alternative-id":["S0006291X26008636"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.154099","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Visualization and detection of ciliary beating frequency in human airway organoids based on image correlated methods for drug screening","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.154099","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"154099"}}