{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:48:39Z","timestamp":1792097319139,"version":"4.4.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100031478","name":"NextGenerationEU","doi-asserted-by":"publisher","award":["20224BYR59"],"award-info":[{"award-number":["20224BYR59"]}],"id":[{"id":"10.13039\/100031478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007052","name":"University of Verona","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.bbrc.2026.154669","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:58:12Z","timestamp":1790693892000},"page":"154669","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Molecular recognition of myosin L by the Toxoplasma gondii calmodulin"],"prefix":"10.1016","volume":"838","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9858-8866","authenticated-orcid":false,"given":"Nicola","family":"Mas\u00e8","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2964-1157","authenticated-orcid":false,"given":"Filippo","family":"Favretto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4953-8349","authenticated-orcid":false,"given":"Silvia","family":"Fruncillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-6295","authenticated-orcid":false,"given":"Silvia N.J.","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7341-0970","authenticated-orcid":false,"given":"Alessandra","family":"Astegno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2026.154669_bib1","doi-asserted-by":"crossref","first-page":"4983","DOI":"10.1038\/s41467-021-25309-9","article-title":"In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems","volume":"12","author":"Mageswaran","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2026.154669_bib2","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1038\/s41467-023-37327-w","article-title":"Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery","volume":"14","author":"Gui","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2026.154669_bib3","article-title":"Cryo-electron tomography of Toxoplasma gondii indicates that the conoid fiber may be derived from microtubules","volume":"10","author":"Li","year":"2023","journal-title":"Advanced science (Weinheim, Baden-Wurttemberg, Germany)"},{"key":"10.1016\/j.bbrc.2026.154669_bib4","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41594-025-01728-w","article-title":"Atomic models of the toxoplasma cell invasion machinery","volume":"33","author":"Zeng","year":"2026","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.bbrc.2026.154669_bib5","article-title":"MyoL-Dependent coupling of preconoidal rings to conoid is required for motility in Toxoplasma gondii","author":"Haase","year":"2026","journal-title":"bioRxiv"},{"key":"10.1016\/j.bbrc.2026.154669_bib6","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/s42003-020-01283-8","article-title":"Structural role of essential light chains in the apicomplexan glideosome","volume":"3","author":"Pazicky","year":"2020","journal-title":"Commun. Biol."},{"key":"10.1016\/j.bbrc.2026.154669_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01363-22","article-title":"Essential functions of calmodulin and identification of its proximal interacting proteins in tachyzoite-stage Toxoplasma gondii via BioID technology","volume":"10","author":"Song","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.bbrc.2026.154669_bib8","volume":"vol.122","author":"Li","year":"2025"},{"key":"10.1016\/j.bbrc.2026.154669_bib9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1042\/BCJ20190674","article-title":"SAC3B is a target of CML19, the centrin 2 of Arabidopsis thaliana","volume":"477","author":"Pedretti","year":"2020","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2026.154669_bib10","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.ijbiomac.2017.11.044","article-title":"Binding of calcium and target peptide to calmodulin-like protein CML19, the centrin 2 of Arabidopsis thaliana","volume":"108","author":"La Verde","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"issue":"13","key":"10.1016\/j.bbrc.2026.154669_bib11","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1042\/BCJ20210295","article-title":"The interplay of self-assembly and target binding in centrin 1 from Toxoplasma gondii","volume":"478","author":"Conter","year":"2021","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2026.154669_bib12","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/abio.2002.5661","article-title":"Measurement of Ca2+-binding constants of proteins and presentation of the CaLigator software","volume":"305","author":"Andre","year":"2002","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bbrc.2026.154669_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.plaphy.2023.108003","article-title":"Role of myristoylation in modulating PCaP1 interaction with calmodulin","volume":"203","author":"Pedretti","year":"2023","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.bbrc.2026.154669_bib14","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/0006-291X(82)90712-4","article-title":"Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography","volume":"104","author":"Gopalakrishna","year":"1982","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2026.154669_bib15","doi-asserted-by":"crossref","first-page":"E1216","DOI":"10.1073\/pnas.1600385113","article-title":"Conserved properties of individual Ca2+-binding sites in calmodulin","volume":"113","author":"Halling","year":"2016","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.bbrc.2026.154669_bib16","doi-asserted-by":"crossref","first-page":"4512","DOI":"10.1105\/tpc.113.113183","article-title":"Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins","volume":"25","author":"Gifford","year":"2013","journal-title":"Plant Cell"},{"key":"10.1016\/j.bbrc.2026.154669_bib17","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.1021\/bi00471a022","article-title":"A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin","volume":"29","author":"Ikura","year":"1990","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2026.154669_bib18","doi-asserted-by":"crossref","first-page":"5450","DOI":"10.1021\/bi972083a","article-title":"Thermodynamic analysis of calcium and magnesium binding to calmodulin","volume":"37","author":"Gilli","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2026.154669_bib19","doi-asserted-by":"crossref","first-page":"8050","DOI":"10.1016\/S0021-9258(18)92938-8","article-title":"Calcium binding to calmodulin and its globular domains","volume":"266","author":"Linse","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2026.154669_bib20","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1002\/pro.5560060406","article-title":"Intermolecular tuning of calmodulin by target peptides and proteins: differential effects on Ca2+ binding and implications for kinase activation","volume":"6","author":"Peersen","year":"1997","journal-title":"Protein Sci."},{"key":"10.1016\/j.bbrc.2026.154669_bib21","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.bpj.2017.01.020","article-title":"Opposing intermolecular tuning of Ca(2+) affinity for calmodulin by neurogranin and CaMKII peptides","volume":"112","author":"Zhang","year":"2017","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbrc.2026.154669_bib22","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.bbapap.2015.12.003","article-title":"Biochemical and biophysical characterization of a plant calmodulin: role of the N- and C-lobes in calcium binding, conformational change, and target interaction","volume":"1864","author":"Astegno","year":"2016","journal-title":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"},{"key":"10.1016\/j.bbrc.2026.154669_bib23","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1074\/jbc.271.1.62","article-title":"Different mechanisms for Ca2+ dissociation from complexes of calmodulin with nitric oxide synthase or myosin light chain kinase","volume":"271","author":"Persechini","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2026.154669_bib24","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1074\/jbc.271.2.761","article-title":"Effects of myosin light chain kinase and peptides on Ca2+ exchange with the N- and C-terminal Ca2+ binding sites of calmodulin","volume":"271","author":"Johnson","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2026.154669_bib25","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.bbrc.2021.12.077","article-title":"Rapid kinetics of calcium dissociation from plant calmodulin and calmodulin-like proteins and effect of target peptides","volume":"590","author":"Troilo","year":"2022","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2026.154669_bib26","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ceca.2014.12.010","article-title":"The calcium signaling toolkit of the apicomplexan parasites Toxoplasma gondii and plasmodium spp","volume":"57","author":"Lourido","year":"2015","journal-title":"Cell Calcium"},{"key":"10.1016\/j.bbrc.2026.154669_bib27","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1016\/j.bbamcr.2018.08.004","article-title":"Calcium signaling and the lytic cycle of the apicomplexan parasite Toxoplasma gondii","volume":"1865","author":"Hortua Triana","year":"2018","journal-title":"Biochimica et biophysica acta. Molecular cell research"},{"key":"10.1016\/j.bbrc.2026.154669_bib28","doi-asserted-by":"crossref","DOI":"10.3390\/biom10081142","article-title":"Distinct calcium binding and structural properties of two centrin isoforms from Toxoplasma gondii","volume":"10","author":"Bombardi","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.bbrc.2026.154669_bib29","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nature09022","article-title":"Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in toxoplasma","volume":"465","author":"Lourido","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2026.154669_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003066","article-title":"TgCDPK3 regulates calcium-dependent egress of Toxoplasma gondii from host cells","volume":"8","author":"McCoy","year":"2012","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.bbrc.2026.154669_bib31","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19051331","article-title":"Towards understanding plant calcium signaling through calmodulin-like proteins: a biochemical and structural perspective","volume":"19","author":"La Verde","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2026.154669_bib32","doi-asserted-by":"crossref","first-page":"4138","DOI":"10.1021\/jp110816h","article-title":"Histidine E7 dynamics modulates ligand exchange between distal pocket and solvent in AHb1 from Arabidopsis thaliana","volume":"115","author":"Spyrakis","year":"2011","journal-title":"J. Phys. Chem. B"},{"issue":"5","key":"10.1016\/j.bbrc.2026.154669_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006379","article-title":"Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii","volume":"13","author":"Long","year":"2017","journal-title":"PLOS Pathog."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26014348?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26014348?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:36:25Z","timestamp":1792096585000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X26014348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":33,"alternative-id":["S0006291X26014348"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.154669","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular recognition of myosin L by the Toxoplasma gondii calmodulin","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.154669","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"154669"}}