{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T09:17:32Z","timestamp":1778750252589,"version":"3.51.4"},"reference-count":19,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["U01DA050330"],"award-info":[{"award-number":["U01DA050330"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry and Biophysics Reports"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.bbrep.2022.101354","type":"journal-article","created":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T03:07:03Z","timestamp":1663988823000},"page":"101354","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb"],"prefix":"10.1016","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6211-1414","authenticated-orcid":false,"given":"Terence L.","family":"Kirley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8348-5773","authenticated-orcid":false,"given":"Andrew B.","family":"Norman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrep.2022.101354_bib1","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.bbrc.2017.04.117","article-title":"Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate","volume":"487","author":"Wetzel","year":"2017","journal-title":"Biochem. Biophys. Res. commun."},{"key":"10.1016\/j.bbrep.2022.101354_bib2","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.intimp.2014.10.025","article-title":"The effects of a humanized recombinant anti-cocaine monoclonal antibody on the disposition of cocaethylene in mice","volume":"23","author":"Wetzel","year":"2014","journal-title":"Int. Immunopharm."},{"key":"10.1016\/j.bbrep.2022.101354_bib3","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1124\/dmd.114.057034","article-title":"A recombinant humanized anti-cocaine monoclonal antibody inhibits the distribution of cocaine to the brain in rats","volume":"42","author":"Norman","year":"2014","journal-title":"Drug Metab. Dispos.: Bio. Fate Chem."},{"key":"10.1016\/j.bbrep.2022.101354_bib4","doi-asserted-by":"crossref","first-page":"458","DOI":"10.4161\/21645515.2014.990856","article-title":"Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment","volume":"11","author":"Kirley","year":"2015","journal-title":"Hum. Vaccines Immunother."},{"key":"10.1016\/j.bbrep.2022.101354_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2019.112676","article-title":"A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics","volume":"476","author":"Kirley","year":"2020","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbrep.2022.101354_bib6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.bbrc.2020.12.034","article-title":"Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy","volume":"535","author":"Kirley","year":"2021","journal-title":"Biochem. Biophys. Res. commun."},{"key":"10.1016\/j.bbrep.2022.101354_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2021.113103","article-title":"Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy","volume":"496","author":"Kirley","year":"2021","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbrep.2022.101354_bib8","article-title":"Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE","volume":"23","author":"Kirley","year":"2020","journal-title":"Biochem. Biophy. Rep."},{"key":"10.1016\/j.bbrep.2022.101354_bib9","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.bbrc.2020.09.051","article-title":"Multi-domain unfolding of the Fab fragment of a humanized anti-cocaine mAb characterized by non-reducing SDS-PAGE","volume":"533","author":"Kirley","year":"2020","journal-title":"Biochem. Biophys. Res. commun."},{"key":"10.1016\/j.bbrep.2022.101354_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.101689","article-title":"Oxidation of specific tryptophan residues inhibits high-affinity binding of cocaine and its metabolites to a humanized anticocaine mAb","volume":"298","author":"Kirley","year":"2022","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrep.2022.101354_bib11","first-page":"697","article-title":"Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2, Acta crystallographica. Section F","volume":"75","author":"Tan","year":"2019","journal-title":"Struct. Biol. Commun."},{"key":"10.1016\/j.bbrep.2022.101354_bib12","article-title":"Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites","volume":"30","author":"Kirley","year":"2022","journal-title":"Biochem. Biophy. Rep."},{"key":"10.1016\/j.bbrep.2022.101354_bib13","doi-asserted-by":"crossref","first-page":"547","DOI":"10.4161\/mabs.27620","article-title":"Preclinical characterization of an anti-methamphetamine monoclonal antibody for human use","volume":"6","author":"Stevens","year":"2014","journal-title":"mAbs"},{"key":"10.1016\/j.bbrep.2022.101354_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0040518","article-title":"Probing cocaine-antibody interactions in buffer and human serum","volume":"7","author":"Ramakrishnan","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrep.2022.101354_bib15","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1021\/jp0664059","article-title":"Thermodynamic studies on the interaction of antibodies with beta-amyloid peptide","volume":"111","author":"Brockhaus","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bbrep.2022.101354_bib16","article-title":"Novel peptide ligands for antibody purification provide superior clearance of host cell protein impurities","volume":"A 1625","author":"Reese","year":"2020","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.bbrep.2022.101354_bib17","doi-asserted-by":"crossref","first-page":"6321","DOI":"10.1021\/jm501511f","article-title":"Ligand binding thermodynamics in drug discovery: still a hot tip?","volume":"58","author":"Geschwindner","year":"2015","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbrep.2022.101354_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms17020144","article-title":"Insights into protein-ligand interactions: mechanisms, models, and methods","volume":"17","author":"Du","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrep.2022.101354_bib19","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1002\/bip.1970.360091002","article-title":"Enthalpy-entropy compensation phenomena in water solutions of proteins and small molecules: a ubiquitous property of water","volume":"9","author":"Lumry","year":"1970","journal-title":"Biopolymers"}],"container-title":["Biochemistry and Biophysics Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580822001546?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580822001546?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T07:48:24Z","timestamp":1762501704000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405580822001546"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":19,"alternative-id":["S2405580822001546"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrep.2022.101354","relation":{},"ISSN":["2405-5808"],"issn-type":[{"value":"2405-5808","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb","name":"articletitle","label":"Article Title"},{"value":"Biochemistry and Biophysics Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrep.2022.101354","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"101354"}}