{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:51:54Z","timestamp":1781117514244,"version":"3.54.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003288","name":"Bell Canada Enterprises","doi-asserted-by":"publisher","award":["001\/2025"],"award-info":[{"award-number":["001\/2025"]}],"id":[{"id":"10.13039\/501100003288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008994","name":"Universidade de Bras\u00edlia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008994","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008431","name":"Department of Primary Industries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"publisher","award":["03\/2025 \\u2013 FAPDF PUBLICA"],"award-info":[{"award-number":["03\/2025 \\u2013 FAPDF PUBLICA"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry and Biophysics Reports"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.bbrep.2025.102128","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T02:31:13Z","timestamp":1752892273000},"page":"102128","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["DODAB-ODN lipoplex structure is highly dependent on ODN concentration: A multitechnique experimental study"],"prefix":"10.1016","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8995-9730","authenticated-orcid":false,"given":"Cristofher Victor","family":"Vivas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6280-8772","authenticated-orcid":false,"given":"Antonio R.","family":"da Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6616-6546","authenticated-orcid":false,"given":"M. Teresa","family":"Lamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5550-6541","authenticated-orcid":false,"given":"Evandro L.","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2135-7423","authenticated-orcid":false,"given":"Gabriel S.","family":"Vignoli Muniz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrep.2025.102128_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2022.120375","article-title":"Gene therapy: comprehensive overview and therapeutic applications","volume":"294","author":"Sayed","year":"2022","journal-title":"Life Sci."},{"key":"10.1016\/j.bbrep.2025.102128_bib2","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1304769","article-title":"Editorial: non-viral vectors for gene therapy\/nucleic acid delivery","volume":"11","author":"Poddar","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.bbrep.2025.102128_bib3","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1208\/s12248-021-00608-7","article-title":"Non-viral vectors in gene therapy: recent development, challenges, and prospects","volume":"23","author":"Zu","year":"2021","journal-title":"AAPS J."},{"key":"10.1016\/j.bbrep.2025.102128_bib4","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jconrel.2015.10.045","article-title":"Composites of malonic acid diamides and phospholipids \u2014 impact of lipoplex stability on transfection efficiency","volume":"220","author":"Janich","year":"2015","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.bbrep.2025.102128_bib5","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.bmc.2016.10.024","article-title":"Lipid-modified oligonucleotide conjugates: insights into gene silencing, interaction with model membranes and cellular uptake mechanisms","volume":"25","author":"Ugarte-Uribe","year":"2017","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.bbrep.2025.102128_bib6","doi-asserted-by":"crossref","DOI":"10.1038\/srep29197","article-title":"Cationic lipid-nanoceria hybrids, a novel nonviral vector-mediated gene delivery into mammalian cells: investigation of the cellular uptake mechanism","volume":"6","author":"Das","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrep.2025.102128_bib7","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.jconrel.2011.10.017","article-title":"Stable assemblies of cationic bilayer fragments and CpG oligonucleotide with enhanced immunoadjuvant activity in vivo","volume":"160","author":"Rozenfeld","year":"2012","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.bbrep.2025.102128_bib8","doi-asserted-by":"crossref","first-page":"6977","DOI":"10.1021\/am500793y","article-title":"Dioctadecyldimethylammonium:Monoolein nanocarriers for efficient in vitro gene silencing","volume":"6","author":"Oliveira","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.bbrep.2025.102128_bib9","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.bbamem.2005.10.011","article-title":"Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6\u2032-dibehenate)\u2014A novel adjuvant inducing both strong CMI and antibody responses","volume":"1718","author":"Davidsen","year":"2005","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."},{"key":"10.1016\/j.bbrep.2025.102128_bib10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.2217\/imt.13.175","article-title":"Optimizing NKT cell ligands as vaccine adjuvants","volume":"6","author":"Carre\u00f1o","year":"2014","journal-title":"Immunotherapy"},{"key":"10.1016\/j.bbrep.2025.102128_bib11","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.1016\/j.bbamem.2011.07.042","article-title":"Structural characterization of the interaction of the polyene antibiotic amphotericin B with DODAB bicelles and vesicles","volume":"1808","author":"Oliveira","year":"2011","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."},{"key":"10.1016\/j.bbrep.2025.102128_bib12","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.jcis.2005.06.046","article-title":"Assembly of a model hydrophobic drug into cationic bilayer fragments","volume":"293","author":"Vieira","year":"2006","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.bbrep.2025.102128_bib13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ejpb.2014.11.028","article-title":"DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system","volume":"89","author":"Carneiro","year":"2015","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.bbrep.2025.102128_bib14","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.3390\/molecules25235741","article-title":"Effect of DODAB nano-sized cationic bilayer fragments against Leishmania amazonensis","volume":"25","author":"Ferreira","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.bbrep.2025.102128_bib15","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.bbamem.2018.12.018","article-title":"Structural characterization of cationic DODAB bilayers containing C24:1 \u03b2-glucosylceramide","volume":"1861","author":"Martins","year":"2019","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."},{"key":"10.1016\/j.bbrep.2025.102128_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemphyslip.2020.104963","article-title":"Supramolecular organization of \u03b1-galactosylceramide in pure dispersions and in cationic DODAB bilayers","volume":"232","author":"Martins","year":"2020","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.bbrep.2025.102128_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.bpc.2023.107075","article-title":"DODAB vesicles containing lysophosphatidylcholines: the relevance of acyl chain saturation on the membrane structure and thermal properties","volume":"300","author":"Martins","year":"2023","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bbrep.2025.102128_bib18","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jbiotec.2016.04.027","article-title":"Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA","volume":"228","author":"Aps","year":"2016","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.bbrep.2025.102128_bib19","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2147\/IJN.S9035","article-title":"Biomimetic nanoparticles: preparation, characterization and biomedical applications","author":"Carmona-Ribeiro","year":"2010","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.bbrep.2025.102128_bib20","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1021\/acs.langmuir.8b04139","article-title":"DNA delivery systems based on peptide-mimicking cationic lipids\u2014the effect of the Co-Lipid on the structure and DNA binding capacity","volume":"35","author":"Tassler","year":"2019","journal-title":"Langmuir"},{"key":"10.1016\/j.bbrep.2025.102128_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2022\/3085103","article-title":"Physicochemical characterization and viability assays of a promising formulation of liposomes (DODAB-DOPC) in complexation with ctDNA","volume":"2022","author":"Ochoa-S\u00e1nchez","year":"2022","journal-title":"J. Nanomater."},{"key":"10.1016\/j.bbrep.2025.102128_bib22","doi-asserted-by":"crossref","first-page":"7498","DOI":"10.1039\/C4CP05652C","article-title":"The effect of an oligonucleotide on the structure of cationic DODAB vesicles","volume":"17","author":"Rozenfeld","year":"2015","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.bbrep.2025.102128_bib23","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jphotochem.2019.03.006","article-title":"A closer look into laurdan as a probe to monitor cationic DODAB bilayers","volume":"376","author":"Masukawa","year":"2019","journal-title":"J. Photochem. Photobiol. Chem."},{"key":"10.1016\/j.bbrep.2025.102128_bib24","series-title":"Principles of Colloid and Surface Chemistry","volume":"3","author":"Hiemenz","year":"1997"},{"key":"10.1016\/j.bbrep.2025.102128_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemphyslip.2022.105173","article-title":"What different physical techniques can disclose about disruptions on membrane structure caused by the antimicrobial peptide hylin a1 and a more positively charged analogue","volume":"243","author":"Vignoli Muniz","year":"2022","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.bbrep.2025.102128_bib26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.aca.2013.05.051","article-title":"The inner filter effects and their correction in fluorescence spectra of salt marsh humic matter","volume":"788","author":"Mendon\u00e7a","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.bbrep.2025.102128_bib27","series-title":"Nanomater. Drug Deliv. Ther.","first-page":"91","article-title":"Multifunctional nanocrystals for cancer therapy: a potential nanocarrier","author":"Joseph","year":"2019"},{"key":"10.1016\/j.bbrep.2025.102128_bib28","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1063\/1.432528","article-title":"Theory of dynamic light scattering from polydisperse systems","volume":"64","author":"Arag\u00f3n","year":"1976","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.bbrep.2025.102128_bib29","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.bbamem.2010.11.036","article-title":"Interaction of cationic bilayer fragments with a model oligonucleotide","volume":"1808","author":"Rozenfeld","year":"2011","journal-title":"Biochim. Biophys. Acta BBA - Biomembr"},{"key":"10.1016\/j.bbrep.2025.102128_bib30","series-title":"Elements of X-ray Diffraction","author":"Cullity","year":"2001"},{"key":"10.1016\/j.bbrep.2025.102128_bib31","series-title":"Methods Enzymol.","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/B978-0-12-381268-1.00016-1","article-title":"Small-angle X-ray scattering studies of peptide\u2013lipid interactions using the mouse paneth cell \u03b1-Defensin Cryptdin-4","author":"Mishra","year":"2011"},{"key":"10.1016\/j.bbrep.2025.102128_bib32","series-title":"Small Angle x-ray Scattering","year":"1982"},{"key":"10.1016\/j.bbrep.2025.102128_bib33","doi-asserted-by":"crossref","DOI":"10.5935\/0103-5053.20150297","article-title":"Structural parameters of lamellar phases formed by the self-assembly of dialkyldimethylammonium bromides in aqueous solution","author":"Ferreira","year":"2015","journal-title":"J. Braz. Chem. Soc."},{"key":"10.1016\/j.bbrep.2025.102128_bib34","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.bbamem.2007.12.005","article-title":"Influence of salt on the structure of DMPG studied by SAXS and optical microscopy","volume":"1778","author":"Fernandez","year":"2008","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."},{"key":"10.1016\/j.bbrep.2025.102128_bib35","series-title":"Chem. Biol.","first-page":"47","article-title":"Differential scanning calorimetry in the study of lipid structures","author":"Go\u00f1i","year":"2006"},{"key":"10.1016\/j.bbrep.2025.102128_bib36","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1007\/s00396-005-1347-9","article-title":"Thermal transitions of DODAB vesicular dispersions","volume":"283","author":"Cocquyt","year":"2005","journal-title":"Colloid Polym. Sci."},{"key":"10.1016\/j.bbrep.2025.102128_bib37","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.jcis.2009.06.054","article-title":"Rheological investigation of thermal transitions in vesicular dispersion","volume":"338","author":"Coppola","year":"2009","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.bbrep.2025.102128_bib38","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1038\/s41598-018-19899-6","article-title":"Dynamical transitions and diffusion mechanism in DODAB bilayer","volume":"8","author":"Dubey","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrep.2025.102128_bib39","article-title":"Comparing the interaction of the antibiotic levofloxacin with zwitterionic and anionic membranes: calorimetry, fluorescence, and spin label studies","volume":"1863","author":"Vignoli Muniz","year":"2021","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."},{"key":"10.1016\/j.bbrep.2025.102128_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0090228","article-title":"N-acylated peptides derived from human lactoferricin perturb organization of cardiolipin and phosphatidylethanolamine in cell membranes and induce defects in Escherichia coli cell division","volume":"9","author":"Zweytick","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrep.2025.102128_bib41","series-title":"Fluoresc. Methods Study Biol. Membr.","first-page":"3","article-title":"LAURDAN fluorescence properties in membranes: a journey from the fluorometer to the microscope","author":"Bagatolli","year":"2012"},{"key":"10.1016\/j.bbrep.2025.102128_bib42","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1023\/A:1020528716621","article-title":"Laurdan and prodan as polarity-sensitive fluorescent membrane probes","volume":"8","author":"Parasassi","year":"1998","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbrep.2025.102128_bib43","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1021\/acs.accounts.0c00687","article-title":"LAURDAN since weber: the quest for visualizing membrane heterogeneity","volume":"54","author":"Gunther","year":"2021","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.bbrep.2025.102128_bib44","series-title":"Molecular Fluorescence: Principles and Applications","author":"Valeur","year":"2002"},{"key":"10.1016\/j.bbrep.2025.102128_bib45","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.1021\/bi00581a025","article-title":"Synthesis and spectral properties of a hydrophobic fluorescent probe: 6-propionyl-2-(dimethylamino)naphthalene","volume":"18","author":"Weber","year":"1979","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrep.2025.102128_bib46","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/s10895-005-0059-3","article-title":"Laurdan in fluid bilayers: position and structural sensitivity","volume":"16","author":"De Vequi-Suplicy","year":"2006","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbrep.2025.102128_bib47","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s10895-015-1545-x","article-title":"New insights on the fluorescent emission spectra of prodan and laurdan","volume":"25","author":"Vequi-Suplicy","year":"2015","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbrep.2025.102128_bib48","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0006-3495(91)82041-0","article-title":"Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of laurdan fluorescence","volume":"60","author":"Parasassi","year":"1991","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbrep.2025.102128_bib49","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s10895-009-0569-5","article-title":"Laurdan spectrum decomposition as a tool for the analysis of surface bilayer structure and polarity: a study with DMPG, peptides and cholesterol","volume":"20","author":"L\u00facio","year":"2010","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbrep.2025.102128_bib50","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0005-2736(03)00112-3","article-title":"Quantifying molecular partition into model systems of biomembranes: an emphasis on optical spectroscopic methods","volume":"1612","author":"Santos","year":"2003","journal-title":"Biochim. Biophys. Acta BBA - Biomembr."}],"container-title":["Biochemistry and Biophysics Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580825002158?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580825002158?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T14:29:36Z","timestamp":1767191376000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405580825002158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":50,"alternative-id":["S2405580825002158"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrep.2025.102128","relation":{},"ISSN":["2405-5808"],"issn-type":[{"value":"2405-5808","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"DODAB-ODN lipoplex structure is highly dependent on ODN concentration: A multitechnique experimental study","name":"articletitle","label":"Article Title"},{"value":"Biochemistry and Biophysics Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrep.2025.102128","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102128"}}