{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:14:22Z","timestamp":1778883262961,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011981","name":"American College of Radiation Oncology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011981","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K10944"],"award-info":[{"award-number":["23K10944"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K14999"],"award-info":[{"award-number":["21K14999"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K06059"],"award-info":[{"award-number":["21K06059"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K08814"],"award-info":[{"award-number":["24K08814"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K09369"],"award-info":[{"award-number":["24K09369"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry and Biophysics Reports"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.bbrep.2026.102489","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:12:12Z","timestamp":1770891132000},"page":"102489","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down"],"prefix":"10.1016","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7259-4248","authenticated-orcid":false,"given":"Masaki","family":"Saito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5930-1954","authenticated-orcid":false,"given":"Wataru","family":"Otsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Ishibashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen-ichi","family":"Atsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrep.2026.102489_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2023.1232188","article-title":"Recent advances in the understanding of cilia mechanisms and their applications as therapeutic targets","volume":"10","author":"Saito","year":"2023","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.bbrep.2026.102489_bib2","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.cell.2007.04.035","article-title":"HEF1-dependent Aurora A activation induces disassembly of the primary cilium","volume":"129","author":"Pugacheva","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib3","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0092-8674(79)90261-7","article-title":"Centriole ciliation is related to quiescence and DNA synthesis in 3T3 cells","volume":"17","author":"Tucker","year":"1979","journal-title":"Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/s41576-023-00587-9","article-title":"Primary cilia as dynamic and diverse signalling hubs in development and disease","volume":"24","author":"Mill","year":"2023","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.bbrep.2026.102489_bib5","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.devcel.2013.07.014","article-title":"IGF-1 activates a cilium-localized noncanonical G\u03b2\u03b3 signaling pathway that regulates cell-cycle progression","volume":"26","author":"Yeh","year":"2013","journal-title":"Dev. Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib6","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/ncb2218","article-title":"Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors","volume":"13","author":"Li","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbrep.2026.102489_bib7","doi-asserted-by":"crossref","first-page":"32281","DOI":"10.1074\/jbc.271.50.32281","article-title":"The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein","volume":"271","author":"King","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrep.2026.102489_bib8","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.bbrc.2003.09.157","article-title":"PTH\/PTH-related protein receptor interacts directly with Tctex-1 through its COOH terminus","volume":"311","author":"Sugai","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrep.2026.102489_bib9","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S0092-8674(00)80800-4","article-title":"Rhodopsin's carboxy-terminal cytoplasmic tail acts as a membrane receptor for cytoplasmic dynein by binding to the dynein light chain Tctex-1","volume":"97","author":"Tai","year":"1999","journal-title":"Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib10","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.devcel.2005.04.003","article-title":"The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth","volume":"9","author":"Chuang","year":"2005","journal-title":"Dev. Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fped.2025.1603050","article-title":"Genetic and clinical insights into MAST4-related neurodevelopmental disorders","volume":"13","author":"Zheng","year":"2025","journal-title":"Front. Pediatr."},{"key":"10.1016\/j.bbrep.2026.102489_bib12","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2023.1097553","article-title":"De novo variants in MAST4 related to neurodevelopmental disorders with developmental delay and infantile spasms: genotype-phenotype association","volume":"16","author":"Zhang","year":"2023","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.bbrep.2026.102489_bib13","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.13390","article-title":"MAST4 controls cell cycle in spermatogonial stem cells","volume":"56","author":"Lee","year":"2023","journal-title":"Cell Prolif."},{"key":"10.1016\/j.bbrep.2026.102489_bib14","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.202301947","article-title":"MAST4 promotes primary ciliary resorption through phosphorylation of Tctex-1","volume":"6","author":"Sakaji","year":"2023","journal-title":"Life Sci. Alliance"},{"key":"10.1016\/j.bbrep.2026.102489_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2021.755867","article-title":"Primary cilia and centrosomes in neocortex development","volume":"15","author":"Wilsch-Brauninger","year":"2021","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.bbrep.2026.102489_bib16","doi-asserted-by":"crossref","first-page":"5816","DOI":"10.1038\/s41467-020-19658-0","article-title":"RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis","volume":"11","author":"Farooq","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrep.2026.102489_bib17","doi-asserted-by":"crossref","first-page":"2612","DOI":"10.1038\/s41467-019-10497-2","article-title":"Modeling microcephaly with cerebral organoids reveals a WDR62-CEP170-KIF2A pathway promoting cilium disassembly in neural progenitors","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrep.2026.102489_bib18","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.1523\/JNEUROSCI.1849-18.2018","article-title":"Cenpj regulates cilia disassembly and neurogenesis in the developing mouse cortex","volume":"39","author":"Ding","year":"2019","journal-title":"J. Neurosci."},{"key":"10.1016\/j.bbrep.2026.102489_bib19","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/S0306-4522(01)00016-1","article-title":"Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex","volume":"103","author":"Tabata","year":"2001","journal-title":"Neuroscience"},{"key":"10.1016\/j.bbrep.2026.102489_bib20","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1073\/pnas.2235688100","article-title":"Electroporation and RNA interference in the rodent retina in vivo and in vitro","volume":"101","author":"Matsuda","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrep.2026.102489_bib21","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1083\/jcb.200908133","article-title":"Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans","volume":"188","author":"Cevik","year":"2010","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbrep.2026.102489_bib22","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1091\/mbc.e09-10-0907","article-title":"Chloride intracellular channel 4 is critical for the epithelial morphogenesis of RPE cells and retinal attachment","volume":"21","author":"Chuang","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.bbrep.2026.102489_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0038174","article-title":"miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons","volume":"7","author":"Pathania","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrep.2026.102489_bib24","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1083\/jcb.200910096","article-title":"The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence","volume":"188","author":"Follit","year":"2010","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbrep.2026.102489_bib25","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.15252\/embr.201744204","article-title":"Tctex-1 controls ciliary resorption by regulating branched actin polymerization and endocytosis","volume":"18","author":"Saito","year":"2017","journal-title":"EMBO Rep."},{"key":"10.1016\/j.bbrep.2026.102489_bib26","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s13041-020-00571-y","article-title":"Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults","volume":"13","author":"Sterpka","year":"2020","journal-title":"Mol. Brain"},{"key":"10.1016\/j.bbrep.2026.102489_bib27","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ydbio.2014.11.009","article-title":"A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation","volume":"397","author":"Lu","year":"2015","journal-title":"Dev. Biol."},{"key":"10.1016\/j.bbrep.2026.102489_bib28","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.1038\/emboj.2012.144","article-title":"Identification of a novel Wnt5a-CK1\u025b-Dvl2-Plk1-mediated primary cilia disassembly pathway","volume":"31","author":"Lee","year":"2012","journal-title":"EMBO J."},{"key":"10.1016\/j.bbrep.2026.102489_bib29","doi-asserted-by":"crossref","DOI":"10.3791\/1938","article-title":"The subventricular zone en-face: wholemount staining and ependymal flow","author":"Mirzadeh","year":"2010","journal-title":"J. Vis. Exp."}],"container-title":["Biochemistry and Biophysics Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S240558082600049X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S240558082600049X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T21:46:22Z","timestamp":1778881582000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S240558082600049X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":29,"alternative-id":["S240558082600049X"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrep.2026.102489","relation":{},"ISSN":["2405-5808"],"issn-type":[{"value":"2405-5808","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down","name":"articletitle","label":"Article Title"},{"value":"Biochemistry and Biophysics Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrep.2026.102489","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102489"}}