{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:20:48Z","timestamp":1777900848738,"version":"3.51.4"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008732","name":"The Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K19156"],"award-info":[{"award-number":["22K19156"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H02936"],"award-info":[{"award-number":["20H02936"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry and Biophysics Reports"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.bbrep.2026.102530","type":"journal-article","created":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:47:47Z","timestamp":1772272067000},"page":"102530","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Preventive and inhibitory effects of Salacia on sustained contraction of vascular smooth muscle cells and characterization of the active compounds"],"prefix":"10.1016","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9043-5515","authenticated-orcid":false,"given":"Katsuko","family":"Kajiya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Inokuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumemi","family":"Sakakibara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideko","family":"Takamatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0491-8612","authenticated-orcid":false,"given":"Hiroki","family":"Yamanouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrep.2026.102530_bib1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1161\/01.RES.87.3.195","article-title":"Involvement of Rho-kinase-mediated phosphorylation of myosin light chain in enhancement of cerebral vasospasm","volume":"87","author":"Sato","year":"2000","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib2","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1161\/01.RES.0000026057.13161.42","article-title":"Sphingosylphosphorylcholine is a novel messenger for Rho-kinase-mediated Ca2+ sensitization in the bovine cerebral artery: unimportant role for protein kinase C","volume":"91","author":"Shirao","year":"2002","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib3","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1152\/physrev.00023.2003","article-title":"Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase","volume":"83","author":"Somlyo","year":"2003","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbrep.2026.102530_bib4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0014-5793(00)02046-9","article-title":"Sphingosylphosphorylcholine induces Ca2+-sensitization of vascular smooth muscle contraction: possible involvement of Rho-kinase","volume":"482","author":"Todoroki-Ikeda","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrep.2026.102530_bib5","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1083\/jcb.143.3.637","article-title":"Polyunsaturated fatty acids inhibit T cell signal transduction by modification of detergent-insoluble membrane domains","volume":"143","author":"Stulnig","year":"1998","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbrep.2026.102530_bib6","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1074\/jbc.275.1.261","article-title":"Inhibition of protein palmitoylation, raft localization, and T cell signaling by 2-bromopalmitate and polyunsaturated fatty acids","volume":"275","author":"Webb","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrep.2026.102530_bib7","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1161\/01.RES.0000042702.04920.BF","article-title":"Involvement of Src family protein tyrosine kinases in Ca2+ sensitization of coronary artery contraction mediated by a sphingosylphosphorylcholine-Rho-kinase pathway","volume":"91","author":"Nakao","year":"2002","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib8","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1002\/biof.1798","article-title":"Fisetin, a major component derived from mulberry (Morus australis poir.) leaves, prevents vascular abnormal contraction","volume":"48","author":"Tsurudome","year":"2022","journal-title":"Biofactors"},{"key":"10.1016\/j.bbrep.2026.102530_bib9","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.drudis.2011.11.007","article-title":"Bridging solubility between drug discovery and development","volume":"17","author":"Di","year":"2012","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.bbrep.2026.102530_bib10","article-title":"Drug solubility: importance and enhancement techniques","volume":"2012","author":"Savjani","year":"2012","journal-title":"ISRN Pharm."},{"key":"10.1016\/j.bbrep.2026.102530_bib11","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1093\/jn\/132.7.1819","article-title":"Salacia reticulata and its polyphenolic constituents with lipase inhibitory and lipolytic activities have mild antiobesity effects in rats","volume":"132","author":"Yoshikawa","year":"2002","journal-title":"J. Nutr."},{"key":"10.1016\/j.bbrep.2026.102530_bib12","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1016\/j.lfs.2004.04.013","article-title":"Salacia oblonga improves cardiac fibrosis and inhibits postprandial hyperglycemia in obese Zucker rats","volume":"75","author":"Li","year":"2004","journal-title":"Life Sci."},{"key":"10.1016\/j.bbrep.2026.102530_bib13","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1111\/j.1463-1326.2007.00750.x","article-title":"Salacia oblonga root decreases cardiac hypertrophy in Zucker diabetic fatty rats: inhibition of cardiac expression of angiotensin II type 1 receptor","volume":"10","author":"Huang","year":"2008","journal-title":"Diabetes Obes. Metabol."},{"key":"10.1016\/j.bbrep.2026.102530_bib14","doi-asserted-by":"crossref","DOI":"10.1093\/ecam\/nep052","article-title":"Preventive effects of Salacia reticulata on obesity and metabolic disorders in TSOD mice","volume":"2011","author":"Akase","year":"2011","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"10.1016\/j.bbrep.2026.102530_bib15","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/aps.2009.28","article-title":"Calcineurin-NFAT signaling is involved in phenylephrine-induced vascular smooth muscle cell proliferation","volume":"30","author":"Pang","year":"2009","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.bbrep.2026.102530_bib16","doi-asserted-by":"crossref","first-page":"C769","DOI":"10.1152\/ajpcell.00529.2004","article-title":"Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus","volume":"288","author":"Ratz","year":"2005","journal-title":"Am. J. Physiol., Cell Physiol."},{"key":"10.1016\/j.bbrep.2026.102530_bib17","article-title":"A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana","volume":"38","author":"Brooks","year":"2023","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.bbrep.2026.102530_bib18","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1080\/1028415X.2017.1354543","article-title":"Pharmacological effects of protocatechuic acid and its therapeutic potential in neurodegenerative diseases: review on the basis of in vitro and in vivo studies in rodents and humans","volume":"22","author":"Krzysztoforska","year":"2019","journal-title":"Nutr. Neurosci."},{"key":"10.1016\/j.bbrep.2026.102530_bib19","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/593902","article-title":"Pharmacological properties of protocatechuic acid and its potential roles as complementary medicine","volume":"2015","author":"Semaming","year":"2015","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"10.1016\/j.bbrep.2026.102530_bib20","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.2174\/092986712800672102","article-title":"Protocatechuic acid and human disease prevention: biological activities and molecular mechanisms","volume":"19","author":"Masella","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.bbrep.2026.102530_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2020.105109","article-title":"New progress in the pharmacology of protocatechuic acid: a compound ingested in daily foods and herbs frequently and heavily","volume":"161","author":"Song","year":"2020","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib22","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.lfs.2017.05.015","article-title":"Protocatechuic acid inhibits hepatitis B virus replication by activating ERK1\/2 pathway and down-regulating HNF4\u03b1 and HNF1\u03b1 in vitro","volume":"180","author":"Dai","year":"2017","journal-title":"Life Sci."},{"key":"10.1016\/j.bbrep.2026.102530_bib23","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1186\/s12906-017-1962-2","article-title":"Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?","volume":"17","author":"Di Nunzio","year":"2017","journal-title":"BMC Compl. Alternative Med."},{"key":"10.1016\/j.bbrep.2026.102530_bib24","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1034\/j.1600-0773.2002.910505.x","article-title":"In vitro cytotoxicity of protocatechuic acid to cultured human cells from oral tissue: involvement in oxidative stress","volume":"91","author":"Babich","year":"2002","journal-title":"Pharmacol. Toxicol."},{"key":"10.1016\/j.bbrep.2026.102530_bib25","doi-asserted-by":"crossref","DOI":"10.1002\/mnfr.70012","article-title":"Protocatechuic acid reduces liver fatty acid uptake in HFD-fed mice associated with the inhibition of DHHC5-mediated CD36 palmitoylation","volume":"69","author":"Li","year":"2025","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib26","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1080\/09637486.2019.1585763","article-title":"Chlorogenic acid-enriched green coffee bean extract affects arterial stiffness assessed by the cardio-ankle vascular index in healthy men: a pilot study","volume":"70","author":"Suzuki","year":"2019","journal-title":"Int. J. Food Sci. Nutr."},{"key":"10.1016\/j.bbrep.2026.102530_bib27","doi-asserted-by":"crossref","first-page":"350","DOI":"10.3109\/09637486.2015.1007453","article-title":"Coffee bean polyphenols ameliorate postprandial endothelial dysfunction in healthy male adults","volume":"66","author":"Ochiai","year":"2015","journal-title":"Int. J. Food Sci. Nutr."},{"key":"10.1016\/j.bbrep.2026.102530_bib28","doi-asserted-by":"crossref","first-page":"525","DOI":"10.3390\/nu10040525","article-title":"Effects of chlorogenic acid-enriched and hydroxyhydroquinone-reduced coffee on postprandial fat oxidation and antioxidative capacity in healthy men: a randomized, double-blind, placebo-controlled, crossover trial","volume":"10","author":"Katada","year":"2018","journal-title":"Nutrients"},{"key":"10.1016\/j.bbrep.2026.102530_bib29","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1271\/bbb.130147","article-title":"Stimulation of postprandial fat utilization in healthy humans by daily consumption of chlorogenic acids","volume":"77","author":"Soga","year":"2013","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.bbrep.2026.102530_bib30","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1017\/S0007114517000587","article-title":"Effects of subacute ingestion of chlorogenic acids on sleep architecture and energy metabolism through activity of the autonomic nervous system: a randomised, placebo-controlled, double-blinded cross-over trial","volume":"117","author":"Park","year":"2017","journal-title":"Br. J. Nutr."},{"key":"10.1016\/j.bbrep.2026.102530_bib31","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/8608497","article-title":"Effect of chlorogenic acid intake on cognitive function in the elderly: a pilot study","volume":"2018","author":"Kato","year":"2018","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"10.1016\/j.bbrep.2026.102530_bib32","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.3390\/nu10101337","article-title":"Effect of chlorogenic acids on cognitive function: a randomized, double-blind, placebo-controlled trial","volume":"10","author":"Saitou","year":"2018","journal-title":"Nutrients"},{"key":"10.1016\/j.bbrep.2026.102530_bib33","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1080\/09168451.2017.1345614","article-title":"Coffee polyphenols extracted from green coffee beans improve skin properties and microcirculatory function","volume":"81","author":"Fukagawa","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.bbrep.2026.102530_bib34","doi-asserted-by":"crossref","first-page":"415","DOI":"10.6090\/jarq.44.415","article-title":"Analysis of six caffeic acid derivatives in sweetpotato leaves by high-performance liquid chromatography using a short column","volume":"44","author":"Okuno","year":"2010","journal-title":"Jpn. Agric. Res. Q."},{"key":"10.1016\/j.bbrep.2026.102530_bib35","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.3390\/foods13111660","article-title":"Formation of cysteine adducts with chlorogenic acid in coffee beans","volume":"13","author":"Sagu","year":"2024","journal-title":"Foods"},{"key":"10.1016\/j.bbrep.2026.102530_bib36","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/carcin\/bgn282","article-title":"Caffeic acid, a phenolic phytochemical in coffee, directly inhibits Fyn kinase activity and UVB-induced COX-2 expression","volume":"30","author":"Kang","year":"2009","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.bbrep.2026.102530_bib37","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1093\/cvr\/cvp084","article-title":"Salicylates dilate blood vessels through inhibiting PYK2-mediated RhoA\/Rho-kinase activation","volume":"83","author":"Ying","year":"2009","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.bbrep.2026.102530_bib38","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1126\/science.1215327","article-title":"The ancient drug salicylate directly activates AMP-activated protein kinase","volume":"336","author":"Hawley","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.bbrep.2026.102530_bib39","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1016\/j.bbadis.2016.04.015","article-title":"Investigation of salicylate hepatic responses in comparison with chemical analogues of the drug","volume":"862","author":"Cameron","year":"2016","journal-title":"Biochim. Biophys. Acta, Mol. Basis Dis."},{"key":"10.1016\/j.bbrep.2026.102530_bib40","first-page":"43","volume":"34","author":"Menendez","year":"2009","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.bbrep.2026.102530_bib41","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.3390\/foods10123053","article-title":"Synergetic inactivation mechanism of protocatechuic acid and high hydrostatic pressure against Escherichia coli O157:H7","volume":"10","author":"Hao","year":"2021","journal-title":"Foods"},{"key":"10.1016\/j.bbrep.2026.102530_bib42","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3390\/beverages5010011","article-title":"Consumption of chlorogenic acids through coffee and health implications","volume":"5","author":"Farah","year":"2019","journal-title":"Beverages"},{"key":"10.1016\/j.bbrep.2026.102530_bib43","doi-asserted-by":"crossref","first-page":"4777","DOI":"10.1039\/D3FO00605K","article-title":"Dietary protocatechuic acid redistributes tight junction proteins by targeting Rho-associated protein kinase to improve intestinal barrier function","volume":"10","author":"So","year":"2023","journal-title":"Food Funct."},{"key":"10.1016\/j.bbrep.2026.102530_bib44","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1021\/acs.jafc.9b02155","article-title":"Low-molecular weight metabolites from polyphenols as effectors for attenuating neuroinflammation","volume":"68","author":"Carregosa","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.bbrep.2026.102530_bib45","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.3390\/antiox9121263","article-title":"Plant phenolics: bioavailability as a key determinant of their potential health-promoting applications","volume":"9","author":"Cosme","year":"2020","journal-title":"Antioxidants"}],"container-title":["Biochemistry and Biophysics Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580826000907?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405580826000907?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:43:29Z","timestamp":1777632209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405580826000907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":45,"alternative-id":["S2405580826000907"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrep.2026.102530","relation":{},"ISSN":["2405-5808"],"issn-type":[{"value":"2405-5808","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preventive and inhibitory effects of Salacia on sustained contraction of vascular smooth muscle cells and characterization of the active compounds","name":"articletitle","label":"Article Title"},{"value":"Biochemistry and Biophysics Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrep.2026.102530","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102530"}}