{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T14:19:28Z","timestamp":1793283568754,"version":"4.5.0"},"reference-count":58,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21OC0069896"],"award-info":[{"award-number":["NNF21OC0069896"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["R346-2020-1829"],"award-info":[{"award-number":["R346-2020-1829"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000291","name":"Kidney Research UK","doi-asserted-by":"publisher","award":["RP_005_20220704"],"award-info":[{"award-number":["RP_005_20220704"]}],"id":[{"id":"10.13039\/501100000291","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003554","name":"Lundbeck Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011958","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","award":["1030-00050A"],"award-info":[{"award-number":["1030-00050A"]}],"id":[{"id":"10.13039\/501100011958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical Pharmacology"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.bcp.2024.116619","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T00:30:53Z","timestamp":1731112253000},"page":"116619","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Detailed functional characterization of four nanobodies as positive allosteric modulators of the human calcium-sensing receptor"],"prefix":"10.1016","volume":"231","author":[{"given":"Wei","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina N.","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor","family":"Barker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Br\u00e4uner-Osborne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper M.","family":"Mathiesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald T.","family":"Ward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7927-5052","authenticated-orcid":false,"given":"Anders A.","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bcp.2024.116619_b0005","doi-asserted-by":"crossref","unstructured":"K. Leach, F.M. Hannan, T.M. Josephs, A.N. Keller, T.C. M\u00f8ller, D.T. Ward, E. Kallay, R.S. Mason, R.V. Thakker, D. Riccardi, A.D. Conigrave, H. Br\u00e4uner-Osborne, International Union of Basic and Clinical Pharmacology. CVIII. Calcium-sensing receptor nomenclature, pharmacology, and function, Pharmacol Rev 72(3) (2020) 558-604.","DOI":"10.1124\/pr.119.018531"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0010","doi-asserted-by":"crossref","first-page":"169","DOI":"10.2174\/138945007779315614","article-title":"Structure, pharmacology and therapeutic prospects of family C G-protein coupled receptors","volume":"8","author":"Br\u00e4uner-Osborne","year":"2007","journal-title":"Curr Drug Targets"},{"key":"10.1016\/j.bcp.2024.116619_b0015","doi-asserted-by":"crossref","unstructured":"S.P.H. Alexander, A. Christopoulos, A.P. Davenport, E. Kelly, A.A. Mathie, J.A. Peters, E.L. Veale, J.F. Armstrong, E. Faccenda, S.D. Harding, J.A. Davies, M.P. Abbracchio, G. Abraham, A. Agoulnik, W. Alexander, K. Al-Hosaini, M. B\u00e4ck, J.G. Baker, N.M. Barnes, R. Bathgate, J.M. Beaulieu, A.G. Beck-Sickinger, M. Behrens, K.E. Bernstein, B. Bettler, N.J.M. Birdsall, V. Blaho, F. Boulay, C. Bousquet, H. Br\u00e4uner-Osborne, G. Burnstock, G. Cal\u00f3, J.P. Casta\u00f1o, K.J. Catt, S. Ceruti, P. Chazot, N. Chiang, B. Chini, J. Chun, A. Cianciulli, O. Civelli, L.H. Clapp, R. Couture, H.M. Cox, Z. Csaba, C. Dahlgren, G. Dent, S.D. Douglas, P. Dournaud, S. Eguchi, E. Escher, E.J. Filardo, T. Fong, M. Fumagalli, R.R. Gainetdinov, M.L. Garelja, M. de Gasparo, C. Gerard, M. Gershengorn, F. Gobeil, T.L. Goodfriend, C. Goudet, L. Gr\u00e4tz, K.J. Gregory, A.L. Gundlach, J. Hamann, J. Hanson, R.L. Hauger, D.L. Hay, A. Heinemann, D. Herr, M.D. Hollenberg, N.D. Holliday, M. Horiuchi, D. Hoyer, L. Hunyady, A. Husain, I.J. AP, T. Inagami, K.A. Jacobson, R.T. Jensen, R. Jockers, D. Jonnalagadda, S. Karnik, K. Kaupmann, J. Kemp, C. Kennedy, Y. Kihara, T. Kitazawa, P. Kozielewicz, H.J. Kreienkamp, J.P. Kukkonen, T. Langenhan, D. Larhammar, K. Leach, D. Lecca, J.D. Lee, S.E. Leeman, J. Leprince, X.X. Li, S.J. Lolait, A. Lupp, R. Macrae, J. Maguire, D. Malfacini, J. Mazella, C.A. McArdle, S. Melmed, M.C. Michel, L.J. Miller, V. Mitolo, B. Mouillac, C.E. M\u00fcller, P.M. Murphy, J.L. Nahon, T. Ngo, X. Norel, D. Nyimanu, A.M. O'Carroll, S. Offermanns, M.A. Panaro, M. Parmentier, R.G. Pertwee, J.P. Pin, E.R. Prossnitz, M. Quinn, R. Ramachandran, M. Ray, R.K. Reinscheid, P. Rondard, G.E. Rovati, C. Ruzza, G.J. Sanger, T. Sch\u00f6neberg, G. Schulte, S. Schulz, D.L. Segaloff, C.N. Serhan, K.D. Singh, C.M. Smith, L.A. Stoddart, Y. Sugimoto, R. Summers, V.P. Tan, D. Thal, W.W. Thomas, P. Timmermans, K. Tirupula, L. Toll, G. Tulipano, H. Unal, T. Unger, C. Valant, P. Vanderheyden, D. Vaudry, H. Vaudry, J.P. Vilardaga, C.S. Walker, J.M. Wang, D.T. Ward, H.J. Wester, G.B. Willars, T.L. Williams, T.M. Woodruff, C. Yao, R.D. Ye, The Concise Guide to PHARMACOLOGY 2023\/24: G protein-coupled receptors, Br J Pharmacol 180 Suppl 2 (2023) S23-s144.","DOI":"10.1111\/bph.16177"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0020","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.beem.2013.05.010","article-title":"Calcium-sensing receptor (CaSR): pharmacological properties and signaling pathways","volume":"27","author":"Conigrave","year":"2013","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"issue":"7867","key":"10.1016\/j.bcp.2024.116619_b0025","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/s41586-021-03691-0","article-title":"Asymmetric activation of the calcium-sensing receptor homodimer","volume":"595","author":"Gao","year":"2021","journal-title":"Nature"},{"issue":"8001","key":"10.1016\/j.bcp.2024.116619_b0030","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/s41586-024-07055-2","article-title":"Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor","volume":"626","author":"He","year":"2024","journal-title":"Nature"},{"issue":"8011","key":"10.1016\/j.bcp.2024.116619_b0035","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/s41586-024-07331-1","article-title":"Promiscuous G-protein activation by the calcium-sensing receptor","volume":"629","author":"Zuo","year":"2024","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.bcp.2024.116619_b0040","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/s41422-023-00892-2","article-title":"Structural insights into asymmetric activation of the calcium-sensing receptor-Gq complex","volume":"34","author":"Ling","year":"2024","journal-title":"Cell Res"},{"issue":"2","key":"10.1016\/j.bcp.2024.116619_b0045","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ceca.2011.11.009","article-title":"Biased agonism of the calcium-sensing receptor","volume":"51","author":"Thomsen","year":"2012","journal-title":"Cell Calcium"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0050","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1124\/mol.119.116772","article-title":"Calcium-sensing receptor internalization is \u03b2-arrestin-dependent and modulated by allosteric ligands","volume":"96","author":"Mos","year":"2019","journal-title":"Mol Pharmacol"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0055","doi-asserted-by":"crossref","first-page":"F485","DOI":"10.1152\/ajprenal.00608.2009","article-title":"Physiology and pathophysiology of the calcium-sensing receptor in the kidney","volume":"298","author":"Riccardi","year":"2010","journal-title":"Am J Physiol Renal Physiol"},{"key":"10.1016\/j.bcp.2024.116619_b0060","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.coemr.2020.06.007","article-title":"Calcium-sensing receptor signaling - how human disease informs biology","volume":"16","author":"Gorvin","year":"2021","journal-title":"Curr Opin Endocr Metab Res"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0065","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41574-018-0115-0","article-title":"The calcium-sensing receptor in physiology and in calcitropic and noncalcitropic diseases","volume":"15","author":"Hannan","year":"2018","journal-title":"Nat Rev Endocrinol"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0070","doi-asserted-by":"crossref","first-page":"R127","DOI":"10.1530\/JME-16-0124","article-title":"Disorders of the calcium-sensing receptor and partner proteins: insights into the molecular basis of calcium homeostasis","volume":"57","author":"Hannan","year":"2016","journal-title":"J Mol Endocrinol"},{"issue":"6","key":"10.1016\/j.bcp.2024.116619_b0075","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.semnephrol.2014.10.001","article-title":"Calcium-sensing receptor activation in chronic kidney disease: effects beyond parathyroid hormone control","volume":"34","author":"Massy","year":"2014","journal-title":"Semin Nephrol"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0080","doi-asserted-by":"crossref","first-page":"6242","DOI":"10.1038\/s41467-023-41770-0","article-title":"Distinct and targetable role of calcium-sensing receptor in leukaemia","volume":"14","author":"Pereira","year":"2023","journal-title":"Nat Commun"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0085","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1124\/jpet.120.000281","article-title":"Characterization of negative allosteric modulators of the calcium-sensing receptor for repurposing as a treatment of asthma","volume":"376","author":"Yarova","year":"2021","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1016\/j.bcp.2024.116619_b0090","doi-asserted-by":"crossref","first-page":"1059369","DOI":"10.3389\/fphys.2022.1059369","article-title":"The calcium-sensing receptor in inflammation: Recent updates","volume":"13","author":"Iamartino","year":"2022","journal-title":"Front Physiol"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0095","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1055\/s-0043-1770353","article-title":"Bone and chronic kidney disease","volume":"27","author":"Lafage-Proust","year":"2023","journal-title":"Semin Musculoskelet Radiol"},{"key":"10.1016\/j.bcp.2024.116619_b0100","doi-asserted-by":"crossref","unstructured":"P.L. Yarova, A.L. Stewart, V. Sathish, R.D. Britt, Jr., M.A. Thompson, P.L. AP, M. Freeman, B. Aravamudan, H. Kita, S.C. Brennan, M. Schepelmann, T. Davies, S. Yung, Z. Cholisoh, E.J. Kidd, W.R. Ford, K.J. Broadley, K. Rietdorf, W. Chang, M.E. Bin Khayat, D.T. Ward, C.J. Corrigan, T.W. JP, P.J. Kemp, C.M. Pabelick, Y.S. Prakash, D. Riccardi, Calcium-sensing receptor antagonists abrogate airway hyperresponsiveness and inflammation in allergic asthma, Sci Transl Med 7(284) (2015) 284ra60.","DOI":"10.1126\/scitranslmed.aaa0282"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0105","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1517\/14656566.6.3.441","article-title":"Cinacalcet HCl: a novel therapeutic for hyperparathyroidism","volume":"6","author":"de Francisco","year":"2005","journal-title":"Expert Opin Pharmacother"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0110","doi-asserted-by":"crossref","first-page":"e0195316","DOI":"10.1371\/journal.pone.0195316","article-title":"A novel calcimimetic agent, evocalcet (MT-4580\/KHK7580), suppresses the parathyroid cell function with little effect on the gastrointestinal tract or CYP isozymes in vivo and in vitro","volume":"13","author":"Kawata","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.bcp.2024.116619_b0115","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ejphar.2018.10.021","article-title":"Pharmacology of Parsabiv(\u00ae) (etelcalcetide, ONO-5163\/AMG 416), a novel allosteric modulator of the calcium-sensing receptor, for secondary hyperparathyroidism in hemodialysis patients","volume":"842","author":"Harada","year":"2019","journal-title":"Eur J Pharmacol"},{"issue":"5","key":"10.1016\/j.bcp.2024.116619_b0120","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/cr.2016.36","article-title":"Towards a structural understanding of allosteric drugs at the human calcium-sensing receptor","volume":"26","author":"Leach","year":"2016","journal-title":"Cell Res"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0125","first-page":"1","article-title":"Discovery and development of calcimimetic and calcilytic compounds","volume":"57","author":"Nemeth","year":"2018","journal-title":"Prog Med Chem"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0130","doi-asserted-by":"crossref","first-page":"180","DOI":"10.2174\/157015907781695982","article-title":"Allosteric modulation of the calcium-sensing receptor","volume":"5","author":"Jensen","year":"2007","journal-title":"Curr Neuropharmacol"},{"issue":"5","key":"10.1016\/j.bcp.2024.116619_b0135","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s11914-019-00533-x","article-title":"Controversies in the management of secondary hyperparathyroidism in chronic kidney disease","volume":"17","author":"Bellorin-Font","year":"2019","journal-title":"Curr Osteoporos Rep"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0140","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1053\/j.ajkd.2020.02.439","article-title":"Comparative effectiveness of calcimimetic agents for secondary hyperparathyroidism in adults: a systematic review and network meta-analysis","volume":"76","author":"Palmer","year":"2020","journal-title":"Am J Kidney Dis"},{"issue":"7","key":"10.1016\/j.bcp.2024.116619_b0145","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1111\/febs.15515","article-title":"A guide to: generation and design of nanobodies","volume":"288","author":"Muyldermans","year":"2021","journal-title":"FEBS J"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0150","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s13550-021-00750-5","article-title":"Nanobody: a promising toolkit for molecular imaging and disease therapy","volume":"11","author":"Bao","year":"2021","journal-title":"EJNMMI Res"},{"key":"10.1016\/j.bcp.2024.116619_b0155","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ceb.2019.01.003","article-title":"Nanobodies detecting and modulating GPCRs outside in and inside out","volume":"57","author":"Heukers","year":"2019","journal-title":"Curr Opin Cell Biol"},{"key":"10.1016\/j.bcp.2024.116619_b0160","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1146\/annurev-pharmtox-010716-104710","article-title":"Nanobodies to study G protein-coupled receptor structure and function","volume":"57","author":"Manglik","year":"2017","journal-title":"Annu Rev Pharmacol Toxicol"},{"key":"10.1016\/j.bcp.2024.116619_b0165","doi-asserted-by":"crossref","unstructured":"I. Mos, T. Z\u00f6gg, A. Wohlk\u00f6nig, A.M.H. Egholm, S.N. Rahman, E. Pardon, J. Steyaert, H. Br\u00e4uner-Osborne, J.M. Mathiesen, Discovery and pharmacological characterization of nanobodies acting as potent positive allosteric modulators of the calcium-sensing receptor, bioRxiv [Preprint] 2024.07.08.602375 (2024).","DOI":"10.1101\/2024.07.08.602375"},{"issue":"9147","key":"10.1016\/j.bcp.2024.116619_b0170","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0140-6736(98)06434-4","article-title":"A986S polymorphism of the calcium-sensing receptor and circulating calcium concentrations","volume":"353","author":"Cole","year":"1999","journal-title":"Lancet"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0175","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/1098-1004(200010)16:4<281::AID-HUMU1>3.0.CO;2-A","article-title":"Mutations of the calcium-sensing receptor (CASR) in familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia","volume":"16","author":"Hendy","year":"2000","journal-title":"Hum Mutat"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0180","doi-asserted-by":"crossref","DOI":"10.1210\/clinem\/dgz205","article-title":"Heterozygous mutation (Q459R) in the calcium-sensing receptor gene causes familial hypocalciuric hypercalcemia 1 (FHH1)","volume":"105","author":"Boisen","year":"2020","journal-title":"J Clin Endocrinol Metab"},{"issue":"405","key":"10.1016\/j.bcp.2024.116619_b0185","doi-asserted-by":"crossref","first-page":"ra123","DOI":"10.1126\/scisignal.aab4068","article-title":"Distinct profiles of functional discrimination among G proteins determine the actions of G protein-coupled receptors","volume":"8","author":"Masuho","year":"2015","journal-title":"Sci Signal"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0190","doi-asserted-by":"crossref","first-page":"4693","DOI":"10.1038\/s41467-019-12399-9","article-title":"Phosphate acts directly on the calcium-sensing receptor to stimulate parathyroid hormone secretion","volume":"10","author":"Centeno","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.bcp.2024.116619_b0195","doi-asserted-by":"crossref","first-page":"46355","DOI":"10.1038\/srep46355","article-title":"Investigating the molecular mechanism of positive and negative allosteric modulators in the calcium-sensing receptor dimer","volume":"7","author":"Jacobsen","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.bcp.2024.116619_b0200","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-1-4939-2914-6_8","article-title":"Monitoring G protein activation in cells with BRET","volume":"1335","author":"Masuho","year":"2015","journal-title":"Methods Mol Biol"},{"issue":"2","key":"10.1016\/j.bcp.2024.116619_b0205","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/bcpt.13738","article-title":"Delineation of the GPR15 receptor-mediated G\u03b1 protein signalling profile in recombinant mammalian cells","volume":"131","author":"Deng","year":"2022","journal-title":"Basic Clin Pharmacol Toxicol"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0210","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1124\/mol.119.119032","article-title":"Detailed in vitro pharmacological characterization of clinically tested negative allosteric modulators of the metabotropic glutamate receptor 5","volume":"98","author":"Arsova","year":"2020","journal-title":"Mol Pharmacol"},{"issue":"3","key":"10.1016\/j.bcp.2024.116619_b0215","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ceca.2003.10.017","article-title":"Calcium receptor-mediated intracellular signalling","volume":"35","author":"Ward","year":"2004","journal-title":"Cell Calcium"},{"key":"10.1016\/j.bcp.2024.116619_b0220","doi-asserted-by":"crossref","first-page":"4040","DOI":"10.1073\/pnas.95.7.4040","article-title":"Calcimimetics with potent and selective activity on the parathyroid calcium receptor","volume":"95","author":"Nemeth","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"18","key":"10.1016\/j.bcp.2024.116619_b0225","doi-asserted-by":"crossref","first-page":"18990","DOI":"10.1074\/jbc.M400724200","article-title":"Positive and negative allosteric modulators of the Ca2+-sensing receptor interact within overlapping but not identical binding sites in the transmembrane domain","volume":"279","author":"Petrel","year":"2004","journal-title":"J Biol Chem"},{"issue":"10","key":"10.1016\/j.bcp.2024.116619_b0230","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.1073\/pnas.080572297","article-title":"The role of members of the pertussis toxin-sensitive family of G proteins in coupling receptors to the activation of the G protein-gated inwardly rectifying potassium channel","volume":"97","author":"Leaney","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"46","key":"10.1016\/j.bcp.2024.116619_b0235","doi-asserted-by":"crossref","first-page":"47438","DOI":"10.1074\/jbc.M408846200","article-title":"A novel G\u03b1q\/11-selective inhibitor","volume":"279","author":"Takasaki","year":"2004","journal-title":"J Biol Chem"},{"issue":"1","key":"10.1016\/j.bcp.2024.116619_b0240","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1124\/jpet.110.178194","article-title":"Characterization of highly efficacious allosteric agonists of the human calcium-sensing receptor","volume":"337","author":"Ma","year":"2011","journal-title":"J Pharmacol Exp Ther"},{"issue":"2","key":"10.1016\/j.bcp.2024.116619_b0245","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1124\/jpet.103.057273","article-title":"Pharmacodynamics of the type II calcimimetic compound cinacalcet HCl","volume":"308","author":"Nemeth","year":"2004","journal-title":"J Pharmacol Exp Ther"},{"issue":"7","key":"10.1016\/j.bcp.2024.116619_b0250","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1021\/acs.biochem.3c00029","article-title":"Neurotensin receptor allosterism revealed in complex with a biased allosteric modulator","volume":"62","author":"Krumm","year":"2023","journal-title":"Biochemistry"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0255","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.tips.2020.12.005","article-title":"Biased allosteric modulators: new frontiers in GPCR drug discovery","volume":"42","author":"Slosky","year":"2021","journal-title":"Trends Pharmacol Sci"},{"key":"10.1016\/j.bcp.2024.116619_b0260","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.bcp.2018.10.014","article-title":"Nanobody-Fc constructs targeting chemokine receptor CXCR4 potently inhibit signaling and CXCR4-mediated HIV-entry and induce antibody effector functions","volume":"158","author":"Bobkov","year":"2018","journal-title":"Biochem Pharmacol"},{"issue":"11","key":"10.1016\/j.bcp.2024.116619_b0265","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.105343","article-title":"Bispecific soluble cytokine receptor-nanobody fusions inhibit Interleukin (IL-)6 trans-signaling and IL-12\/23 or tumor necrosis factor (TNF) signaling","volume":"299","author":"Gesiorowski","year":"2023","journal-title":"J Biol Chem"},{"issue":"4","key":"10.1016\/j.bcp.2024.116619_b0270","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1111\/j.1476-5381.2010.00742.x","article-title":"Kinetic analysis of novel mono- and multivalent VHH-fragments and their application for molecular imaging of brain tumours","volume":"160","author":"Iqbal","year":"2010","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.bcp.2024.116619_b0275","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2024.05.027","article-title":"A novel inhalable nanobody targeting IL-4R\u03b1 for the treatment of asthma","author":"Zhu","year":"2024","journal-title":"J Allergy Clin Immunol"},{"issue":"11","key":"10.1016\/j.bcp.2024.116619_b0280","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.1096\/fj.14-253369","article-title":"A novel platform for engineering blood-brain barrier-crossing bispecific biologics","volume":"28","author":"Farrington","year":"2014","journal-title":"Faseb j"},{"key":"10.1016\/j.bcp.2024.116619_b0285","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.freeradbiomed.2022.02.031","article-title":"Nanobodies as molecular imaging probes","volume":"182","author":"Barakat","year":"2022","journal-title":"Free Radic Biol Med"},{"issue":"W1","key":"10.1016\/j.bcp.2024.116619_b0290","doi-asserted-by":"crossref","first-page":"W521","DOI":"10.1093\/nar\/gkae241","article-title":"The EMBL-EBI Job Dispatcher sequence analysis tools framework in 2024","volume":"52","author":"Madeira","year":"2024","journal-title":"Nucleic Acids Res"}],"container-title":["Biochemical Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006295224006191?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006295224006191?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:59:48Z","timestamp":1777377588000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006295224006191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":58,"alternative-id":["S0006295224006191"],"URL":"https:\/\/doi.org\/10.1016\/j.bcp.2024.116619","relation":{},"ISSN":["0006-2952"],"issn-type":[{"value":"0006-2952","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detailed functional characterization of four nanobodies as positive allosteric modulators of the human calcium-sensing receptor","name":"articletitle","label":"Article Title"},{"value":"Biochemical Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bcp.2024.116619","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116619"}}