{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T21:28:51Z","timestamp":1778880531178,"version":"3.51.4"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["NE160\/7-1"],"award-info":[{"award-number":["NE160\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SPP2170"],"award-info":[{"award-number":["SPP2170"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical Engineering Journal"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.bej.2023.108905","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T21:58:57Z","timestamp":1679522337000},"page":"108905","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Controlling Aspergillus niger morphology in a low shear-force environment in a rocking-motion bioreactor"],"prefix":"10.1016","volume":"195","author":[{"given":"Tolue","family":"Kheirkhah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6185-2627","authenticated-orcid":false,"given":"Stefan","family":"Junne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bej.2023.108905_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40694-016-0024-8","article-title":"Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper","volume":"3","author":"Meyer","year":"2016","journal-title":"Fungal Biol. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib2","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1007\/s00449-018-1932-1","article-title":"Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing","volume":"41","author":"Sun","year":"2018","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.bej.2023.108905_bib3","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1080\/07388551.2018.1531821","article-title":"A current approach to the control of filamentous fungal growth in media: microparticle enhanced cultivation technique","volume":"39","author":"Karahalil","year":"2019","journal-title":"Crit. Rev. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/elsc.202100094","article-title":"Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form","volume":"22","author":"Soerjawinata","year":"2022","journal-title":"Eng. Life Sci."},{"key":"10.1016\/j.bej.2023.108905_bib5","doi-asserted-by":"crossref","first-page":"844","DOI":"10.4014\/jmb.0911.11020","article-title":"Effects of dissolved oxygen on fungal morphology and process rheology during fed-batch processing of Ganoderma lucidum","volume":"20","author":"Fazenda","year":"2010","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2859-5-3","article-title":"Morphological development of Aspergillus niger in submerged citric acid fermentation as a function of the spore inoculum level. Application of neural network and cluster analysis for characterization of mycelial morphology","volume":"5","author":"Papagianni","year":"2006","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.bej.2023.108905_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.enzmictec.2021.109833","article-title":"Cellulolytic enzymes production guided by morphology engineering","volume":"149","author":"Buffo","year":"2021","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.bej.2023.108905_bib8","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1631\/jzus.B1100039","article-title":"Red pigment production by Penicillium purpurogenum GH2 is influenced by pH and temperature","volume":"12","author":"M\u00e9ndez","year":"2011","journal-title":"J. Zhejiang Univ. Sci. B."},{"key":"10.1016\/j.bej.2023.108905_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2859-10-58","article-title":"Morphology engineering - Osmolality and its effect on Aspergillus niger morphology and productivity","volume":"10","author":"Wucherpfennig","year":"2011","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.bej.2023.108905_bib10","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.bej.2019.04.023","article-title":"The influence of salt-enhanced cultivation on the micromechanical behaviour of filamentous pellets","volume":"148","author":"Dittmann","year":"2019","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.bej.2023.108905_bib11","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s00449-004-0368-y","article-title":"Agitation effects on submerged growth and product formation of Aspergillus niger","volume":"26","author":"Kelly","year":"2004","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.bej.2023.108905_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/antibiotics8040168","article-title":"Characterization of the metabolic response of Streptomyces clavuligerus to shear stress in stirred tanks and single-use 2D rocking motion bioreactors for clavulanic acid production","volume":"8","author":"G\u00f3mez-R\u00edos","year":"2019","journal-title":"Antibiotics"},{"key":"10.1016\/j.bej.2023.108905_bib13","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jbiotec.2004.10.005","article-title":"Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus","volume":"116","author":"Casas L\u00f3pez","year":"2005","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib14","doi-asserted-by":"crossref","first-page":"2997","DOI":"10.1007\/s00253-018-8818-7","article-title":"The filamentous fungal pellet\u2014relationship between morphology and productivity","volume":"102","author":"Veiter","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib15","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1002\/bit.10209","article-title":"Estimation of hyphal tensile strength in production-scale Aspergillus oryzae fungal fermentations","volume":"77","author":"Li","year":"2002","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib16","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s00449-014-1337-8","article-title":"Dependence of fungal characteristics on seed morphology and shear stress in bioreactors","volume":"38","author":"Lu","year":"2015","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.bej.2023.108905_bib17","doi-asserted-by":"crossref","DOI":"10.1186\/s40694-021-00115-6","article-title":"Understanding and controlling filamentous growth of fungal cell factories: novel tools and opportunities for targeted morphology engineering","volume":"8","author":"Meyer","year":"2021","journal-title":"Fungal Biol. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib18","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1007\/s12010-021-03592-y","article-title":"Bioprocess optimisation for high cell density endoinulinase production from recombinant Aspergillus niger","volume":"193","author":"Maumela","year":"2021","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib19","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/9783527683369.ch9","article-title":"Design, applications, and development of single-use bioreactors","author":"Oosterhuis","year":"2016","journal-title":"Bioreact. Des. Oper. Nov. Appl."},{"key":"10.1016\/j.bej.2023.108905_bib20","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1002\/elsc.201400010","article-title":"Growth and docosahexaenoic acid production performance of the heterotrophic marine microalgae Crypthecodinium cohnii in the wave-mixed single-use reactor CELL-tainer","volume":"14","author":"Hillig","year":"2014","journal-title":"Eng. Life Sci."},{"key":"10.1016\/j.bej.2023.108905_bib21","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1002\/cite.201200149","article-title":"Cultivation of cells and microorganisms in wave-mixed disposable bag bioreactors at different scales","volume":"85","author":"Junne","year":"2013","journal-title":"Chem. -Ing. -Tech."},{"key":"10.1016\/j.bej.2023.108905_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-018-0975-y","article-title":"Rocking Aspergillus: Morphology-controlled cultivation of Aspergillus niger in a wave-mixed bioreactor for the production of secondary metabolites","volume":"17","author":"Kurt","year":"2018","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.bej.2023.108905_bib23","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/S0032-9592(02)00002-X","article-title":"Protease secretion in glucoamylase producer Aspergillus niger cultures: Fungal morphology and inoculum effects","volume":"37","author":"Papagianni","year":"2002","journal-title":"Process Biochem."},{"key":"10.1016\/j.bej.2023.108905_bib24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0065-2164(10)72004-9","article-title":"Morphology and rheology in filamentous cultivations","volume":"72","author":"Wucherpfennig","year":"2010","journal-title":"Adv. Appl. Microbiol."},{"key":"10.1016\/j.bej.2023.108905_bib25","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1002\/bit.22614","article-title":"Morphology engineering of Aspergillus niger for improved enzyme production","volume":"105","author":"Driouch","year":"2010","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib26","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1002\/bit.23313","article-title":"Improved enzyme production by bio-pellets of Aspergillus niger: Targeted morphology engineering using titanate microparticles","volume":"109","author":"Driouch","year":"2012","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib27","first-page":"1","article-title":"Customization of Aspergillus niger morphology through addition of talc micro particles","author":"Wucherpfennig","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.bej.2023.108905_bib28","first-page":"145","article-title":"Improving 2-phenylethanol and 6-pentyl-\u03b1-pyrone production with fungi by microparticle-enhanced cultivation (MPEC)","volume":"32","author":"Etschmann","year":"2015","journal-title":"Yeast"},{"key":"10.1016\/j.bej.2023.108905_bib29","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/elsc.201900013","article-title":"Kinetic model to describe the morphological evolution of filamentous fungi during their early stages of growth in the standard submerged and microparticle-enhanced cultivations","volume":"19","author":"Kowalska","year":"2019","journal-title":"Eng. Life Sci."},{"key":"10.1016\/j.bej.2023.108905_bib30","doi-asserted-by":"crossref","DOI":"10.1186\/s40694-014-0004-9","article-title":"Engineering of Aspergillus niger for the production of secondary metabolites","volume":"1","author":"Richter","year":"2014","journal-title":"Fungal Biol. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1128\/br.21.3.195-213.1957","article-title":"The thiobacilli","volume":"21","author":"Vishniac","year":"1957","journal-title":"Bacteriol. Rev."},{"key":"10.1016\/j.bej.2023.108905_bib32","doi-asserted-by":"crossref","DOI":"10.3389\/fceng.2022.1021416","article-title":"CFD modelling of a wave-mixed bioreactor with complex geometry and two degrees of freedom motion","volume":"4","author":"Seidel","year":"2022","journal-title":"Front. Chem. Eng."},{"key":"10.1016\/j.bej.2023.108905_bib33","doi-asserted-by":"crossref","DOI":"10.1186\/s12859-017-1934-z","article-title":"ImageJ2: ImageJ for the next generation of scientific image data","volume":"18","author":"Rueden","year":"2017","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.bej.2023.108905_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/s13068-019-1473-0","article-title":"A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger","volume":"12","author":"Cairns","year":"2019","journal-title":"Biotechnol. Biofuels."},{"key":"10.1016\/j.bej.2023.108905_bib35","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/elsc.201300055","article-title":"Adding talc microparticles to Aspergillus terreus ATCC 20542 preculture decreases fungal pellet size and improves lovastatin production","volume":"14","author":"Gonciarz","year":"2014","journal-title":"Eng. Life Sci."},{"key":"10.1016\/j.bej.2023.108905_bib36","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/bit.21713","article-title":"Microparticle-enhanced cultivation of filamentous microorganisms: increased chloroperoxidase formation by Caldariomyces fumago as an example","volume":"99","author":"Kaup","year":"2008","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib37","article-title":"The taming of the shrew - Controlling the morphology of filamentous eukaryotic and prokaryotic microorganisms","volume":"149","author":"Walisko","year":"2015","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"10.1016\/j.bej.2023.108905_bib38","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/5832496","article-title":"Application of aluminum oxide nanoparticles in Aspergillus terreus cultivations: Evaluating the effects on lovastatin production and fungal morphology","volume":"2019","author":"Boruta","year":"2019","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.bej.2023.108905_bib39","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1007\/s00449-015-1366-y","article-title":"Influence of oxygen on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 quantitatively studied on the level of individual pellets","volume":"38","author":"Bizukojc","year":"2015","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.bej.2023.108905_bib40","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1002\/bit.28124","article-title":"From spores to fungal pellets: A new high-throughput image analysis highlights the structural development of Aspergillus niger","volume":"119","author":"M\u00fcller","year":"2022","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.enzmictec.2020.109587","article-title":"Relation between pellet fragmentation kinetics and cellulolytic enzymes production by Aspergillus niger in conventional bioreactor with different impellers","volume":"139","author":"Buffo","year":"2020","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.bej.2023.108905_bib42","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1002\/1097-0290(20001105)70:3<300::AID-BIT7>3.0.CO;2-3","article-title":"Fungal morphology and fragmentation behavior in a fed-batch Aspergillus oryzae fermentation at the production scale","volume":"70","author":"Li","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bej.2023.108905_bib43","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.bej.2016.07.006","article-title":"Application of Euler\u2013Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor","volume":"114","author":"Liu","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.bej.2023.108905_bib44","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.ces.2018.08.017","article-title":"Study of hydrodynamics in wave bioreactors by computational fluid dynamics reveals a resonance phenomenon","volume":"193","author":"Zhan","year":"2019","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.bej.2023.108905_bib45","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.ces.2014.07.037","article-title":"Effective shear rates in shake flasks","volume":"118","author":"Giese","year":"2014","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.bej.2023.108905_bib46","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.bej.2016.06.001","article-title":"Quantitative evaluation of the shear threshold on Carthamus tinctorius L. cell growth with computational fluid dynamics in shaken flask bioreactors","volume":"113","author":"Liu","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.bej.2023.108905_bib47","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/cite.202000176","article-title":"The impact of fluid dynamic stress in stirred bioreactors \u2013 the scale of the biological entity: a personal view","volume":"93","author":"Nienow","year":"2021","journal-title":"Chem. -Ing. -Tech."},{"key":"10.1016\/j.bej.2023.108905_bib48","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1002\/bit.260351009","article-title":"The effect of agitation on the morphology and penicillin production of Penicillium chrysogenum","volume":"35","author":"Smith","year":"1990","journal-title":"Biotechnol. Bioeng."}],"container-title":["Biochemical Engineering Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1369703X23001006?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1369703X23001006?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:31:46Z","timestamp":1778805106000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1369703X23001006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":48,"alternative-id":["S1369703X23001006"],"URL":"https:\/\/doi.org\/10.1016\/j.bej.2023.108905","relation":{},"ISSN":["1369-703X"],"issn-type":[{"value":"1369-703X","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Controlling Aspergillus niger morphology in a low shear-force environment in a rocking-motion bioreactor","name":"articletitle","label":"Article Title"},{"value":"Biochemical Engineering Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bej.2023.108905","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"108905"}}