{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T21:21:47Z","timestamp":1778793707604,"version":"3.51.4"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0112100"],"award-info":[{"award-number":["2019YFA0112100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402825"],"award-info":[{"award-number":["82402825"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82220108005"],"award-info":[{"award-number":["82220108005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92468205"],"award-info":[{"award-number":["92468205"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010104","name":"Tianjin Medical University","doi-asserted-by":"publisher","award":["2023FY05"],"award-info":[{"award-number":["2023FY05"]}],"id":[{"id":"10.13039\/501100010104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioactive Materials"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.bioactmat.2026.01.024","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T09:35:27Z","timestamp":1769765727000},"page":"401-424","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair"],"prefix":"10.1016","volume":"60","author":[{"given":"Jie","family":"Ren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongda","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwen","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaying","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengyu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanhao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomeng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangzhi","family":"Ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqing","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bioactmat.2026.01.024_bib1","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2017.18","article-title":"Traumatic spinal cord injury","volume":"3","author":"Ahuja","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"issue":"8","key":"10.1016\/j.bioactmat.2026.01.024_bib2","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1089\/neu.2011.2226","article-title":"The health and life priorities of individuals with spinal cord injury: a systematic review","volume":"29","author":"Simpson","year":"2012","journal-title":"J. Neurotrauma"},{"issue":"5","key":"10.1016\/j.bioactmat.2026.01.024_bib3","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/nn.4541","article-title":"Cell transplantation therapy for spinal cord injury","volume":"20","author":"Assinck","year":"2017","journal-title":"Nat. Neurosci."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/s41413-022-00199-9","article-title":"Progression in translational research on spinal cord injury based on microenvironment imbalance","volume":"10","author":"Fan","year":"2022","journal-title":"Bone Res."},{"issue":"6","key":"10.1016\/j.bioactmat.2026.01.024_bib5","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1177\/0963689718755778","article-title":"Microenvironment imbalance of spinal cord injury","volume":"27","author":"Fan","year":"2018","journal-title":"Cell Transplant."},{"issue":"7","key":"10.1016\/j.bioactmat.2026.01.024_bib6","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.ijdevneu.2013.07.004","article-title":"Review of transplantation of neural stem\/progenitor cells for spinal cord injury","volume":"31","author":"Mothe","year":"2013","journal-title":"Int. J. Dev. Neurosci. : the official journal of the International Society for Developmental Neuroscience"},{"issue":"16","key":"10.1016\/j.bioactmat.2026.01.024_bib7","doi-asserted-by":"crossref","DOI":"10.3390\/cells12162040","article-title":"Microglia negatively regulate the proliferation and neuronal differentiation of neural stem\/progenitor cells isolated from poststroke mouse brains","volume":"12","author":"Hirano","year":"2023","journal-title":"Cells"},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib8","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.stem.2015.06.007","article-title":"Stem cell therapies in clinical trials: progress and challenges","volume":"17","author":"Trounson","year":"2015","journal-title":"Cell Stem Cell"},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib9","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1186\/s13287-020-02054-2","article-title":"Quantitative assessment of the impact of cryopreservation on human bone marrow-derived mesenchymal stem cells: up to 24 h post-thaw and beyond","volume":"11","author":"Bahsoun","year":"2020","journal-title":"Stem Cell Res. Ther."},{"issue":"3","key":"10.1016\/j.bioactmat.2026.01.024_bib10","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/sctm.18-0121","article-title":"Zooming in on cryopreservation of hiPSCs and neural derivatives: a dual-center study using adherent vitrification","volume":"8","author":"Kaindl","year":"2019","journal-title":"Stem Cells Transl. Med."},{"issue":"7","key":"10.1016\/j.bioactmat.2026.01.024_bib11","doi-asserted-by":"crossref","first-page":"897","DOI":"10.3109\/14653240903253857","article-title":"Ex vivo-expanded autologous bone marrow-derived mesenchymal stromal cells in human spinal cord injury\/paraplegia: a pilot clinical study","volume":"11","author":"Pal","year":"2009","journal-title":"Cytotherapy"},{"issue":"4","key":"10.1016\/j.bioactmat.2026.01.024_bib12","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/sj.bmt.1705731","article-title":"Microbial contamination of hematopoietic progenitor cell products: clinical outcome","volume":"40","author":"Patah","year":"2007","journal-title":"Bone Marrow Transplant."},{"issue":"6","key":"10.1016\/j.bioactmat.2026.01.024_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.stem.2018.05.014","article-title":"A First-in-Human, phase I study of neural stem cell transplantation for chronic spinal cord injury","volume":"22","author":"Curtis","year":"2018","journal-title":"Cell Stem Cell"},{"issue":"12","key":"10.1016\/j.bioactmat.2026.01.024_bib14","article-title":"Long-term clinical and safety outcomes from a single-site phase 1 study of neural stem cell transplantation for chronic thoracic spinal cord injury","volume":"5","author":"Martin","year":"2024","journal-title":"Cell Rep. Med."},{"issue":"4","key":"10.1016\/j.bioactmat.2026.01.024_bib15","doi-asserted-by":"crossref","first-page":"340","DOI":"10.2174\/1574888X15666200316164051","article-title":"Human mesenchymal stem cells for spinal cord injury","volume":"15","author":"Alishahi","year":"2020","journal-title":"Curr. Stem Cell Res. Ther."},{"issue":"11","key":"10.1016\/j.bioactmat.2026.01.024_bib16","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20112698","article-title":"Mesenchymal stem cells for spinal cord injury: current options, limitations, and future of cell therapy","volume":"20","author":"Cofano","year":"2019","journal-title":"Int. J. Mol. Sci."},{"issue":"3","key":"10.1016\/j.bioactmat.2026.01.024_bib17","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3727\/096368915X686841","article-title":"Human umbilical cord mesenchymal stem cells: a new era for stem cell therapy","volume":"24","author":"Ding","year":"2015","journal-title":"Cell Transplant."},{"issue":"4","key":"10.1016\/j.bioactmat.2026.01.024_bib18","doi-asserted-by":"crossref","first-page":"651","DOI":"10.4103\/1673-5374.155442","article-title":"Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms","volume":"10","author":"Guo","year":"2015","journal-title":"Neural Regen. Res."},{"key":"10.1016\/j.bioactmat.2026.01.024_bib19","doi-asserted-by":"crossref","first-page":"6917","DOI":"10.2147\/IJN.S264498","article-title":"Exosome: a review of its classification, isolation techniques, storage, diagnostic and targeted therapy applications","volume":"15","author":"Zhang","year":"2020","journal-title":"Int. J. Nanomed."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib20","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0169-409X(97)00048-3","article-title":"Biodegradation and biocompatibility of PLA and PLGA microspheres","volume":"28","author":"Shive","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib21","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ijpharm.2018.06.015","article-title":"Porous microspheres: synthesis, characterisation and applications in pharmaceutical & medical fields","volume":"548","author":"Ghosh","year":"2018","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.bioactmat.2026.01.024_bib22","first-page":"1111","article-title":"Porous microsphere and its applications","volume":"8","author":"Cai","year":"2013","journal-title":"Int. J. Nanomed."},{"issue":"4","key":"10.1016\/j.bioactmat.2026.01.024_bib23","first-page":"407","article-title":"Injectable hydrogels for spinal cord injury repair","volume":"3","author":"Wang","year":"2022","journal-title":"Eng. Regen."},{"key":"10.1016\/j.bioactmat.2026.01.024_bib24","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.msec.2018.04.006","article-title":"hucMSC derived exosomes promote functional recovery in spinal cord injury mice via attenuating inflammation","volume":"89","author":"Sun","year":"2018","journal-title":"Mater. Sci. Eng., C"},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib25","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/s41420-021-00572-3","article-title":"Human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate the repair of spinal cord injury via the miR-29b-3p\/PTEN\/Akt\/mTOR axis","volume":"7","author":"Xiao","year":"2021","journal-title":"Cell Death Discov."},{"issue":"13","key":"10.1016\/j.bioactmat.2026.01.024_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202105586","article-title":"Exosomes-loaded electroconductive hydrogel synergistically promotes tissue repair after spinal cord injury via immunoregulation and enhancement of myelinated axon growth","volume":"9","author":"Fan","year":"2022","journal-title":"Adv. Sci."},{"key":"10.1016\/j.bioactmat.2026.01.024_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.compositesb.2024.111618","article-title":"Bioactive hydrogels loaded with BMSC-EXOs and GDNF for synergistically spinal cord injury repairing","volume":"284","author":"Cheng","year":"2024","journal-title":"Compos. B Eng."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib28","first-page":"68","article-title":"Spinal cord conduits for spinal cord injury regeneration","volume":"4","author":"Ma","year":"2023","journal-title":"Eng. Regen."},{"issue":"7","key":"10.1016\/j.bioactmat.2026.01.024_bib29","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1038\/s41563-025-02214-w","article-title":"Ultrasound-activated piezoelectric nanostickers for neural stem cell therapy of traumatic brain injury","volume":"24","author":"Wang","year":"2025","journal-title":"Nat. Mater."},{"issue":"21","key":"10.1016\/j.bioactmat.2026.01.024_bib30","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202308993","article-title":"Magnetic nanoparticles and methylprednisolone based physico-chemical bifunctional neural stem cells delivery system for spinal cord injury repair","volume":"11","author":"Zhang","year":"2024","journal-title":"Adv. Sci."},{"key":"10.1016\/j.bioactmat.2026.01.024_bib31","article-title":"Laminin-modified porous GelMA microspheres sustain a pro-neurogenic niche for neural stem cells transplantation in spinal cord injury","author":"Wang","year":"2025","journal-title":"Adv. Funct. Mater."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib32","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1021\/la902166j","article-title":"Surface modification changes the degradation process and degradation product pattern of polylactide","volume":"26","author":"H\u00f6glund","year":"2010","journal-title":"Langmuir"},{"key":"10.1016\/j.bioactmat.2026.01.024_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2023.122137","article-title":"DLP printed hDPSC-loaded GelMA microsphere regenerates dental pulp and repairs spinal cord","volume":"299","author":"Qian","year":"2023","journal-title":"Biomaterials"},{"key":"10.1016\/j.bioactmat.2026.01.024_bib34","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.msec.2018.08.054","article-title":"3D culture of neural stem cells within conductive PEDOT layer-assembled chitosan\/gelatin scaffolds for neural tissue engineering","volume":"93","author":"Wang","year":"2018","journal-title":"Mater. Sci. Eng., C"},{"issue":"18","key":"10.1016\/j.bioactmat.2026.01.024_bib35","doi-asserted-by":"crossref","first-page":"5413","DOI":"10.1111\/febs.16110","article-title":"Regulation of redox signaling in HIF-1-dependent tumor angiogenesis","volume":"289","author":"Manuelli","year":"2022","journal-title":"FEBS J."},{"issue":"5","key":"10.1016\/j.bioactmat.2026.01.024_bib36","doi-asserted-by":"crossref","DOI":"10.3390\/cells12050798","article-title":"Transcriptional response to hypoxia: the role of HIF-1-Associated Co-Regulators","volume":"12","author":"Yfantis","year":"2023","journal-title":"Cells"},{"issue":"5","key":"10.1016\/j.bioactmat.2026.01.024_bib37","first-page":"2969","article-title":"HIF-1-VEGF-Notch mediates angiogenesis in temporomandibular joint osteoarthritis","volume":"11","author":"Chen","year":"2019","journal-title":"Am. J. Tourism Res."},{"issue":"9","key":"10.1016\/j.bioactmat.2026.01.024_bib38","doi-asserted-by":"crossref","DOI":"10.1172\/JCI183607","article-title":"Restoring mitochondrial function promotes hematopoietic reconstitution from cord blood following cryopreservation-related functional decline","volume":"135","author":"Huang","year":"2025","journal-title":"J. Clin. Investig."},{"issue":"3","key":"10.1016\/j.bioactmat.2026.01.024_bib39","first-page":"1363","article-title":"Expert consensus on the application of stem cells in psoriasis research and clinical trials","volume":"16","author":"Kuang","year":"2024","journal-title":"Aging and disease"},{"key":"10.1016\/j.bioactmat.2026.01.024_bib40","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ijbiomac.2021.08.105","article-title":"Anti freeze proteins (Afp): properties, sources and applications - a review","volume":"189","author":"Baskaran","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib41","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3171\/2015.9.SPINE15628","article-title":"Hyaluronic acid scaffold has a neuroprotective effect in hemisection spinal cord injury","volume":"25","author":"Kushchayev","year":"2016","journal-title":"J. Neurosurg. Spine"},{"issue":"25","key":"10.1016\/j.bioactmat.2026.01.024_bib42","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202302498","article-title":"Building-block size mediates microporous annealed particle hydrogel tube microenvironment following spinal cord injury","volume":"13","author":"Ross","year":"2024","journal-title":"Adv. Healthcare Mater."},{"issue":"6","key":"10.1016\/j.bioactmat.2026.01.024_bib43","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1038\/s41591-019-0475-6","article-title":"Spinal cord repair: advances in biology and technology","volume":"25","author":"Courtine","year":"2019","journal-title":"Nat. Med."},{"issue":"11","key":"10.1016\/j.bioactmat.2026.01.024_bib44","doi-asserted-by":"crossref","first-page":"4955","DOI":"10.7150\/thno.108329","article-title":"Biomimetic piezoelectric hydrogel system for energy metabolism reprogramming in spinal cord injury repair","volume":"15","author":"Shi","year":"2025","journal-title":"Theranostics"},{"issue":"7","key":"10.1016\/j.bioactmat.2026.01.024_bib45","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1021\/acs.accounts.9b00109","article-title":"Drug delivery with extracellular vesicles: from imagination to innovation","volume":"52","author":"de Jong","year":"2019","journal-title":"Acc. Chem. Res."},{"issue":"1","key":"10.1016\/j.bioactmat.2026.01.024_bib46","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1186\/s12951-025-03768-2","article-title":"Advances of extracellular vesicles isolation and detection frontier technology: from heterogeneity analysis to clinical application","volume":"23","author":"Li","year":"2025","journal-title":"J. Nanobiotechnol."},{"issue":"11","key":"10.1016\/j.bioactmat.2026.01.024_bib47","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1089\/wound.2024.0093","article-title":"Standardized reporting of research on exosomes to ensure rigor and reproducibility","volume":"13","author":"Yadav","year":"2024","journal-title":"Adv. Wound Care"}],"container-title":["Bioactive Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2452199X26000253?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2452199X26000253?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T08:28:23Z","timestamp":1778315303000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2452199X26000253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":47,"alternative-id":["S2452199X26000253"],"URL":"https:\/\/doi.org\/10.1016\/j.bioactmat.2026.01.024","relation":{},"ISSN":["2452-199X"],"issn-type":[{"value":"2452-199X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair","name":"articletitle","label":"Article Title"},{"value":"Bioactive Materials","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioactmat.2026.01.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}