{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T20:47:08Z","timestamp":1793998028163,"version":"4.8.0"},"reference-count":66,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"am","delay-in-days":229,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100005713","name":"Office of the Secretary of Defense","doi-asserted-by":"publisher","award":["W911NF-17-3-0003"],"award-info":[{"award-number":["W911NF-17-3-0003"]}],"id":[{"id":"10.13039\/100005713","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomaterials Advances"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.bioadv.2025.214288","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T08:21:21Z","timestamp":1742199681000},"page":"214288","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["A functional human liver tissue model: 3D bioprinted co-culture discoids"],"prefix":"10.1016","volume":"173","author":[{"given":"Vignesh","family":"Subramaniam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Abrahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett R.","family":"Higgins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Chisolm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baleigh","family":"Sweeney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthilkumar","family":"Duraivel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Balzano-Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tia","family":"Monjure","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Yi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glyn D.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Angelini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.bioadv.2025.214288_bb0005","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1093\/ilar\/ilw025","article-title":"The promise of new technologies to reduce, refine, or replace animal use while reducing risks of drug induced liver injury in pharmaceutical development","volume":"57","author":"Sistare","year":"2016","journal-title":"ILAR J."},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0010","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1007\/s42242-021-00142-7","article-title":"Recent advances in the development of in vitro liver models for hepatotoxicity testing","volume":"4","author":"Lu","year":"2021","journal-title":"Bio-Des. Manuf."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0015","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13062-020-0259-4","article-title":"Predictability of drug-induced liver injury by machine learning","volume":"15","author":"Chierici","year":"2020","journal-title":"Biol. Direct"},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0020","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1080\/03602530600959615","article-title":"Evaluation of which reactive metabolite, if any, is responsible for a specific idiosyncratic reaction","volume":"38","author":"Uetrecht","year":"2006","journal-title":"Drug Metab. Rev."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0025","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1006\/rtph.2000.1399","article-title":"Concordance of the toxicity of pharmaceuticals in humans and in animals","volume":"32","author":"Olson","year":"2000","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.bioadv.2025.214288_bb0030","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0168-8278(97)80494-1","article-title":"Hepatotoxicity in drug development: detection, significance and solutions","volume":"26","author":"Ballet","year":"1997","journal-title":"J. Hepatol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0035","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1093\/toxsci\/kfv117","article-title":"Morphological and functional characterization and assessment of iPSC-derived hepatocytes for in vitro toxicity testing","volume":"147","author":"Lu","year":"2015","journal-title":"Toxicol. Sci."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0040","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/nbt1361","article-title":"Microscale culture of human liver cells for drug development","volume":"26","author":"Khetani","year":"2008","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.taap.2013.01.012","article-title":"A long-term three dimensional liver co-culture system for improved prediction of clinically relevant drug-induced hepatotoxicity","volume":"268","author":"Kostadinova","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"6","key":"10.1016\/j.bioadv.2025.214288_bb0050","doi-asserted-by":"crossref","first-page":"569","DOI":"10.2174\/138920005774832632","article-title":"A microscale in vitro physiological model of the liver: predictive screens for drug metabolism and enzyme induction","volume":"6","author":"Sivaraman","year":"2005","journal-title":"Curr. Drug Metab."},{"issue":"6","key":"10.1016\/j.bioadv.2025.214288_bb0055","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3109\/10408444.2012.682115","article-title":"Organotypic liver culture models: meeting current challenges in toxicity testing","volume":"42","author":"LeCluyse","year":"2012","journal-title":"Crit. Rev. Toxicol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0060","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1080\/03602530601093489","article-title":"Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies","volume":"39","author":"Hewitt","year":"2007","journal-title":"Drug Metab. Rev."},{"key":"10.1016\/j.bioadv.2025.214288_bb0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-60761-688-7_1","article-title":"General review on in vitro hepatocyte models and their applications","volume":"640","author":"Guguen-Guillouzo","year":"2010","journal-title":"Meth. Mol. Biol."},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0070","doi-asserted-by":"crossref","first-page":"292","DOI":"10.2174\/1389200033489424","article-title":"Human hepatocytes as a tool for studying toxicity and drug metabolism","volume":"4","author":"Gomez-Lechon","year":"2003","journal-title":"Curr. Drug Metab."},{"issue":"8","key":"10.1016\/j.bioadv.2025.214288_bb0075","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1007\/s00204-013-1078-5","article-title":"Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME","volume":"87","author":"Godoy","year":"2013","journal-title":"Arch. Toxicol."},{"issue":"11","key":"10.1016\/j.bioadv.2025.214288_bb0080","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1517\/17425255.2014.967680","article-title":"Competency of different cell models to predict human hepatotoxic drugs","volume":"10","author":"G\u00f3mez-Lech\u00f3n","year":"2014","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0085","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s13287-021-02152-9","article-title":"Advancements in stem cell-derived hepatocyte-like cell models for hepatotoxicity testing","volume":"12","author":"Jin","year":"2021","journal-title":"Stem Cell Res. Ther."},{"issue":"2","key":"10.1016\/j.bioadv.2025.214288_bb0090","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0887-2333(00)00002-3","article-title":"The use of liver spheroids as an in vitro model for studying induction of the stress response as a marker of chemical toxicity","volume":"14","author":"Dilworth","year":"2000","journal-title":"Toxicol. In Vitro"},{"issue":"7","key":"10.1016\/j.bioadv.2025.214288_bb0095","doi-asserted-by":"crossref","first-page":"e0158674","DOI":"10.1371\/journal.pone.0158674","article-title":"Bioprinted 3D primary liver tissues allow assessment of organ-level response to clinical drug induced toxicity in vitro","volume":"11","author":"Nguyen","year":"2016","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0100","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1039\/B913221J","article-title":"Perfused multiwell plate for 3D liver tissue engineering","volume":"10","author":"Domansky","year":"2010","journal-title":"Lab Chip"},{"key":"10.1016\/j.bioadv.2025.214288_bb0105","doi-asserted-by":"crossref","first-page":"e17","DOI":"10.51335\/organoid.2022.2.e17","article-title":"Advanced human liver models for the assessment of drug-induced liver injury","volume":"2","author":"Mun","year":"2022","journal-title":"Organoid"},{"key":"10.1016\/j.bioadv.2025.214288_bb0110","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.biomaterials.2015.08.043","article-title":"A microfluidically perfused three dimensional human liver model","volume":"71","author":"Rennert","year":"2015","journal-title":"Biomaterials"},{"issue":"10","key":"10.1016\/j.bioadv.2025.214288_bb0115","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1517\/17425250903160664","article-title":"Liver tissue engineering in the evaluation of drug safety","volume":"5","author":"Dash","year":"2009","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"issue":"19","key":"10.1016\/j.bioadv.2025.214288_bb0120","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.1002\/adma.201305506","article-title":"3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs","volume":"26","author":"Kolesky","year":"2014","journal-title":"Adv. Mater."},{"issue":"12","key":"10.1016\/j.bioadv.2025.214288_bb0125","doi-asserted-by":"crossref","first-page":"3179","DOI":"10.1073\/pnas.1521342113","article-title":"Three-dimensional bioprinting of thick vascularized tissues","volume":"113","author":"Kolesky","year":"2016","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"10","key":"10.1016\/j.bioadv.2025.214288_bb0130","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/S0142-9612(98)00026-X","article-title":"Proteins and cells on PEG immobilized silicon surfaces","volume":"19","author":"Zhang","year":"1998","journal-title":"Biomaterials"},{"key":"10.1016\/j.bioadv.2025.214288_bb0135","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.procir.2015.11.001","article-title":"Pluronic F127 hydrogel characterization and biofabrication in cellularized constructs for tissue engineering applications","volume":"49","author":"Gioffredi","year":"2016","journal-title":"Proc. CIRP"},{"issue":"10","key":"10.1016\/j.bioadv.2025.214288_bb0140","doi-asserted-by":"crossref","first-page":"6711","DOI":"10.1021\/acsbiomaterials.4c01086","article-title":"Determining rates of molecular secretion from supernatant concentration measurements in a 3D-bioprinted human liver tissue model","volume":"10","author":"Anjum","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.bioadv.2025.214288_bb0145","doi-asserted-by":"crossref","first-page":"100297","DOI":"10.1016\/j.giant.2024.100297","article-title":"Biopolymer networks packed with microgels combine strain stiffening and shape programmability","author":"Subramaniam","year":"2024","journal-title":"Giant"},{"issue":"10","key":"10.1016\/j.bioadv.2025.214288_bb0150","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1021\/acsbiomaterials.6b00218","article-title":"Liquid-like solids support cells in 3D","volume":"2","author":"Bhattacharjee","year":"2016","journal-title":"ACS Biomater Sci. Eng."},{"issue":"9","key":"10.1016\/j.bioadv.2025.214288_bb0155","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1039\/C7SM01518F","article-title":"Polyelectrolyte scaling laws for microgel yielding near jamming","volume":"14","author":"Bhattacharjee","year":"2018","journal-title":"Soft Matter"},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0160","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1038\/s41467-019-10919-1","article-title":"Quantitative characterization of 3D bioprinted structural elements under cell generated forces","volume":"10","author":"Morley","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bioadv.2025.214288_bb0165","doi-asserted-by":"crossref","first-page":"e00121","DOI":"10.1016\/j.bprint.2020.e00121","article-title":"3D printed collagen structures at low concentrations supported by jammed microgels","volume":"21","author":"Zhang","year":"2021","journal-title":"Bioprinting"},{"issue":"45","key":"10.1016\/j.bioadv.2025.214288_bb0170","doi-asserted-by":"crossref","first-page":"8554","DOI":"10.1039\/D2SM01013E","article-title":"Cellular micromasonry: biofabrication with single cell precision","volume":"18","author":"Ellison","year":"2022","journal-title":"Soft Matter"},{"key":"10.1016\/j.bioadv.2025.214288_bb0175","doi-asserted-by":"crossref","first-page":"e00231","DOI":"10.1016\/j.bprint.2022.e00231","article-title":"Spatiotemporal T cell dynamics in a 3D bioprinted immunotherapy model","volume":"28","author":"Morley","year":"2022","journal-title":"Bioprinting"},{"issue":"3","key":"10.1016\/j.bioadv.2025.214288_bb0180","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/abec2c","article-title":"Engineering bioinks for 3D bioprinting","volume":"13","author":"Decante","year":"2021","journal-title":"Biofabrication"},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0185","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s40824-018-0122-1","article-title":"Recent trends in bioinks for 3D printing","volume":"22","author":"Gopinathan","year":"2018","journal-title":"Biomater. Res."},{"issue":"8","key":"10.1016\/j.bioadv.2025.214288_bb0190","doi-asserted-by":"crossref","first-page":"e1500655","DOI":"10.1126\/sciadv.1500655","article-title":"Writing in the granular gel medium","volume":"1","author":"Bhattacharjee","year":"2015","journal-title":"Sci. Adv."},{"issue":"6638","key":"10.1016\/j.bioadv.2025.214288_bb0195","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1126\/science.ade4441","article-title":"A silicone-based support material eliminates interfacial instabilities in 3D silicone printing","volume":"379","author":"Duraivel","year":"2023","journal-title":"Science"},{"issue":"2","key":"10.1016\/j.bioadv.2025.214288_bb0200","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1208\/s12248-008-9037-4","article-title":"Current industrial practices in assessing CYP450 enzyme induction: preclinical and clinical","volume":"10","author":"Sinz","year":"2008","journal-title":"AAPS J."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0205","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/1873-3468.13582","article-title":"Long-term maintenance of functional primary human hepatocytes using small molecules","volume":"594","author":"Katsuda","year":"2020","journal-title":"FEBS Lett."},{"issue":"5","key":"10.1016\/j.bioadv.2025.214288_bb0210","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1440-1746.1991.tb00897.x","article-title":"Hepatocyte growth factor: molecular structure and implications for a central role in liver regeneration","volume":"6","author":"Matsumoto","year":"1991","journal-title":"J. Gastroenterol. Hepatol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0215","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0006-291X(03)01117-3","article-title":"A novel function of connexin 32: marked enhancement of liver function in a hepatoma cell line","volume":"307","author":"Yang","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"2\u20136","key":"10.1016\/j.bioadv.2025.214288_bb0220","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3109\/15419061.2016.1151875","article-title":"Structure, regulation and function of gap junctions in liver","volume":"22","author":"Willebrords","year":"2015","journal-title":"Cell Commun. Adhes."},{"key":"10.1016\/j.bioadv.2025.214288_bb0225","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1146\/annurev-chembioeng-092220-015404","article-title":"Medical 3D printing: tools and techniques, today and tomorrow","volume":"13","author":"Willson","year":"2022","journal-title":"Annu. Rev. Chem. Biomol. Eng."},{"key":"10.1016\/j.bioadv.2025.214288_bb0230","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.msec.2015.12.032","article-title":"Fabrication of scalable tissue engineering scaffolds with dual-pore microarchitecture by combining 3D printing and particle leaching","volume":"61","author":"Mohanty","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0235","doi-asserted-by":"crossref","first-page":"045003","DOI":"10.1088\/1758-5090\/7\/4\/045003","article-title":"A new method of fabricating a blend scaffold using an indirect three-dimensional printing technique","volume":"7","author":"Jung","year":"2015","journal-title":"Biofabrication"},{"issue":"3","key":"10.1016\/j.bioadv.2025.214288_bb0240","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/nbt.3413","article-title":"A 3D bioprinting system to produce human-scale tissue constructs with structural integrity","volume":"34","author":"Kang","year":"2016","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0245","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1089\/ten.2006.0143","article-title":"Identification of differentially expressed genes in hepatocyte\/endothelial cell co-culture system","volume":"13","author":"Takayama","year":"2007","journal-title":"Tissue Eng."},{"issue":"34","key":"10.1016\/j.bioadv.2025.214288_bb0250","doi-asserted-by":"crossref","first-page":"8848","DOI":"10.1016\/j.biomaterials.2011.08.004","article-title":"Human hepatocytes and endothelial cells in organotypic membrane systems","volume":"32","author":"Salerno","year":"2011","journal-title":"Biomaterials"},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0255","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.transproceed.2013.11.086","article-title":"Self-formation of vascularized hepatic tissue from human adult hepatocyte","volume":"46","author":"Enomoto","year":"2014","journal-title":"Transplant. Proc."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0260","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1002\/jcp.1041290115","article-title":"Long-term maintenance of hepatocyte functional activity in co-culture: requirements for sinusoidal endothelial cells and dexamethasone","volume":"129","author":"Morin","year":"1986","journal-title":"J. Cell. Physiol."},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0265","doi-asserted-by":"crossref","first-page":"045008","DOI":"10.1088\/1758-5082\/5\/4\/045008","article-title":"Layered long-term co-culture of hepatocytes and endothelial cells on a transwell membrane: toward engineering the liver sinusoid","volume":"5","author":"Kang","year":"2013","journal-title":"Biofabrication"},{"issue":"6","key":"10.1016\/j.bioadv.2025.214288_bb0270","doi-asserted-by":"crossref","first-page":"065003","DOI":"10.1088\/1468-6996\/14\/6\/065003","article-title":"Micropatterned co-culture of hepatocyte spheroids layered on non-parenchymal cells to understand heterotypic cellular interactions","volume":"14","author":"Otsuka","year":"2013","journal-title":"Sci. Technol. Adv. Mater."},{"key":"10.1016\/j.bioadv.2025.214288_bb0275","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.actbio.2019.07.047","article-title":"3-D culture and endothelial cells improve maturity of human pluripotent stem cell-derived hepatocytes","volume":"95","author":"Ardalani","year":"2019","journal-title":"Acta Biomater."},{"issue":"4","key":"10.1016\/j.bioadv.2025.214288_bb0280","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/(SICI)1097-4652(199812)177:4<553::AID-JCP6>3.0.CO;2-F","article-title":"Long-term expression of differentiated functions in hepatocytes cultured in three-dimensional collagen matrix","volume":"177","author":"G\u00f3mez-Lech\u00f3n","year":"1998","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.bioadv.2025.214288_bb0285","first-page":"11","article-title":"3D bioprinting of dECM-incorporated hepatocyte spheroid for simultaneous promotion of cell-cell and -ECM interactions","author":"Kim","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"5","key":"10.1016\/j.bioadv.2025.214288_bb0290","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1002\/biot.201000326","article-title":"Modular bioreactor for primary human hepatocyte culture: medium flow stimulates expression and activity of detoxification genes","volume":"6","author":"Vinci","year":"2011","journal-title":"Biotechnol. J."},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0295","first-page":"1907952","article-title":"Hepatocyte CYP2B6 can be expressed in cell culture systems by exerting physiological levels of shear: implications for ADME testing","volume":"2017","author":"Hammond","year":"2017","journal-title":"J. Toxicol."},{"issue":"8","key":"10.1016\/j.bioadv.2025.214288_bb0300","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1124\/dmd.32.8.834","article-title":"Effect of dexamethasone treatment on the expression and function of transport proteins in sandwich-cultured rat hepatocytes","volume":"32","author":"Turncliff","year":"2004","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.bioadv.2025.214288_bb0305","first-page":"9","article-title":"How donor and surgical factors affect the viability and functionality of human hepatocytes isolated from liver resections","author":"Solanas","year":"2022","journal-title":"Front. Med."},{"key":"10.1016\/j.bioadv.2025.214288_bb0310","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.tiv.2016.02.013","article-title":"Inter-donor variability of phase I\/phase II metabolism of three reference drugs in cryopreserved primary human hepatocytes in suspension and monolayer","volume":"33","author":"den Braver-Sewradj","year":"2016","journal-title":"Toxicol. In Vitro"},{"issue":"3","key":"10.1016\/j.bioadv.2025.214288_bb0315","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1023\/A:1011944531257","article-title":"Human liver quality is a dominant factor in the outcome of in vitro studies","volume":"17","author":"Fisher","year":"2001","journal-title":"Cell Biol. Toxicol."},{"issue":"10\u201311","key":"10.1016\/j.bioadv.2025.214288_bb0320","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1080\/00498250600861728","article-title":"Compendium of gene expression profiles comprising a baseline model of the human liver drug metabolism transcriptome","volume":"36","author":"Slatter","year":"2006","journal-title":"Xenobiotica"},{"issue":"1","key":"10.1016\/j.bioadv.2025.214288_bb0325","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1093\/biostatistics\/kxj037","article-title":"Adjusting batch effects in microarray expression data using empirical Bayes methods","volume":"8","author":"Johnson","year":"2007","journal-title":"Biostatistics"},{"key":"10.1016\/j.bioadv.2025.214288_bb0330","series-title":"The New S Language: A Programming Environment for Data Analysis and Graphics","author":"Becker","year":"1988"}],"container-title":["Biomaterials Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2772950825001153?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2772950825001153?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T16:15:26Z","timestamp":1750176926000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2772950825001153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":66,"alternative-id":["S2772950825001153"],"URL":"https:\/\/doi.org\/10.1016\/j.bioadv.2025.214288","relation":{},"ISSN":["2772-9508"],"issn-type":[{"value":"2772-9508","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A functional human liver tissue model: 3D bioprinted co-culture discoids","name":"articletitle","label":"Article Title"},{"value":"Biomaterials Advances","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioadv.2025.214288","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"214288"}}