{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T19:15:26Z","timestamp":1769282126677,"version":"3.49.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The International Journal of Biochemistry &amp; Cell Biology"],"published-print":{"date-parts":[[2014,7]]},"DOI":"10.1016\/j.biocel.2014.03.002","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T04:45:10Z","timestamp":1395031510000},"page":"130-135","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"special_numbering":"C","title":["Mammalian short palate lung and nasal epithelial clone 1 (SPLUNC1) in pH-dependent airway hydration"],"prefix":"10.1016","volume":"52","author":[{"given":"Robert","family":"Tarran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew R.","family":"Redinbo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.biocel.2014.03.002_bib0005","doi-asserted-by":"crossref","first-page":"30073","DOI":"10.1074\/jbc.M111.338574","article-title":"Cathepsin B is secreted apically from Xenopus 2F3 cells and cleaves the epithelial sodium channel (ENaC) to increase its activity","volume":"287","author":"Alli","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2014.03.002_bib0010","series-title":"Respiratory defense mechanisms","first-page":"49","author":"Andersen","year":"1977"},{"key":"10.1016\/j.biocel.2014.03.002_bib0015","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1042\/BST0391012","article-title":"PLUNC: a multifunctional surfactant of the airways","volume":"39","author":"Bartlett","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.biocel.2014.03.002_bib0020","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1126\/science.276.5320.1861","article-title":"Crystal structure of human BPI and two bound phospholipids at 2.4 angstrom resolution","volume":"276","author":"Beamer","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.biocel.2014.03.002_bib0025","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1093\/hmg\/11.8.937","article-title":"PLUNC: a novel family of candidate host defence proteins expressed in the upper airways and nasopharynx","volume":"11","author":"Bingle","year":"2002","journal-title":"Hum Mol Genet"},{"key":"10.1016\/j.biocel.2014.03.002_bib0030","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1465-9921-8-79","article-title":"Differential epithelial expression of the putative innate immune molecule SPLUNC1 in cystic fibrosis","volume":"8","author":"Bingle","year":"2007","journal-title":"Respir Res"},{"key":"10.1016\/j.biocel.2014.03.002_bib0035","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1164\/ajrccm.150.1.8025763","article-title":"Human airway ion transport: Part one","volume":"150","author":"Boucher","year":"1994","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0040","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1172\/JCI112708","article-title":"Na+ transport in cystic fibrosis respiratory epithelia. Abnormal basal rate and response to adenylate cyclase activation","volume":"78","author":"Boucher","year":"1986","journal-title":"J Clin Invest"},{"key":"10.1016\/j.biocel.2014.03.002_bib0045","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1126\/science.1223012","article-title":"A periciliary brush promotes the lung health by separating the mucus layer from airway epithelia","volume":"337","author":"Button","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.biocel.2014.03.002_bib0050","doi-asserted-by":"crossref","first-page":"L813","DOI":"10.1152\/ajplung.00435.2004","article-title":"Neutrophil elastase activates near-silent epithelial Na+ channels and increases airway epithelial Na+ transport","volume":"288","author":"Caldwell","year":"2005","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0055","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1002\/pmic.200401172","article-title":"Identification of human nasal mucous proteins using proteomics","volume":"5","author":"Casado","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.biocel.2014.03.002_bib0060","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1465-9921-4-8","article-title":"State of the art: why do the lungs of patients with cystic fibrosis become infected and why can\u2019t they clear the infection?","volume":"4","author":"Chmiel","year":"2003","journal-title":"Respir Res"},{"key":"10.1016\/j.biocel.2014.03.002_bib0065","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/alr.20028","article-title":"Acid and base secretion in freshly excised nasal tissue from cystic fibrosis patients with DeltaF508 mutation","volume":"1","author":"Cho","year":"2011","journal-title":"Int Forum Allergy Rhinol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0070","doi-asserted-by":"crossref","first-page":"16083","DOI":"10.1073\/pnas.2634339100","article-title":"Abnormal surface liquid pH regulation by cultured cystic fibrosis bronchial epithelium","volume":"100","author":"Coakley","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biocel.2014.03.002_bib0075","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1165\/ajrcmb.26.3.4611","article-title":"Protection against acute lung injury by intravenous or intratracheal pretreatment with EPI-HNE-4, a new potent neutrophil elastase inhibitor","volume":"26","author":"Delacourt","year":"2002","journal-title":"Am J Respir Cell Mol Biol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0080","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1042\/BST0391051","article-title":"Functional roles of SPLUNC1 in the innate immune response against Gram-negative bacteria","volume":"39","author":"Di","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.biocel.2014.03.002_bib0085","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1074\/jbc.M210523200","article-title":"Molecular cloning and characterization of spurt, a human novel gene that is retinoic acid-inducible and encodes a secretory protein specific in upper respiratory tracts","volume":"278","author":"Di","year":"2003","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2014.03.002_bib0090","doi-asserted-by":"crossref","first-page":"8338","DOI":"10.1074\/jbc.M105044200","article-title":"Regulation of the epithelial sodium channel by serine proteases in human airways","volume":"277","author":"Donaldson","year":"2002","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2014.03.002_bib0095","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1056\/NEJMra0910061","article-title":"Airway mucus function and dysfunction","volume":"363","author":"Fahy","year":"2010","journal-title":"N Engl J Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0100","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/pupt.1999.0166","article-title":"Effects of dextran on tracheal mucociliary velocity in dogs in vivo","volume":"12","author":"Feng","year":"1999","journal-title":"Pulm Pharmacol Ther"},{"key":"10.1016\/j.biocel.2014.03.002_bib0105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00424-010-0827-z","article-title":"Regulation of the epithelial Na+ channel and airway surface liquid volume by serine proteases","volume":"460","author":"Gaillard","year":"2010","journal-title":"Pflugers Arch"},{"key":"10.1016\/j.biocel.2014.03.002_bib0110","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1016\/j.ajpath.2011.01.026","article-title":"SPLUNC1 promotes lung innate defense against Mycoplasma pneumoniae infection in mice","volume":"178","author":"Gally","year":"2011","journal-title":"Am J Pathol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0115","doi-asserted-by":"crossref","first-page":"11412","DOI":"10.1073\/pnas.0903609106","article-title":"SPLUNC1 regulates airway surface liquid volume by protecting ENaC from proteolytic cleavage","volume":"106","author":"Garcia-Caballero","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biocel.2014.03.002_bib0120","doi-asserted-by":"crossref","first-page":"15973","DOI":"10.1073\/pnas.1311999110","article-title":"Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways","volume":"110","author":"Garland","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biocel.2014.03.002_bib0125","first-page":"L990","article-title":"The cystic fibrosis transmembrane conductance regulator impedes proteolytic stimulation of the epithelial Na+ channel","volume":"305","author":"Gentzsch","year":"2010","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2014.03.002_bib0130","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1164\/rccm.200304-505SO","article-title":"Pathophysiology and management of pulmonary infections in cystic fibrosis","volume":"168","author":"Gibson","year":"2003","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0135","doi-asserted-by":"crossref","DOI":"10.1152\/ajplung.00103.2013","article-title":"Identification of SPLUNC1's ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airway cultures","author":"Hobbs","year":"2013","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0140","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1097\/MNH.0b013e32821f6072","article-title":"Role of proteolysis in the activation of epithelial sodium channels","volume":"16","author":"Hughey","year":"2007","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"10.1016\/j.biocel.2014.03.002_bib0145","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/1097-0215(200002)9999:9999<::AID-IJC1059>3.0.CO;2-B","article-title":"Isolation of a novel human lung-specific gene. LUNX, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer","volume":"91","author":"Iwao","year":"2001","journal-title":"Int J Cancer"},{"key":"10.1016\/j.biocel.2014.03.002_bib0150","doi-asserted-by":"crossref","first-page":"e64689","DOI":"10.1371\/journal.pone.0064689","article-title":"Human neutrophil elastase degrades SPLUNC1 and impairs airway epithelial defense against bacteria","volume":"8","author":"Jiang","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.biocel.2014.03.002_bib0155","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1056\/NEJM199907153410304","article-title":"Pulmonary epithelial sodium-channel dysfunction and excess airway liquid in pseudohypoaldosteronism","volume":"341","author":"Kerem","year":"1999","journal-title":"N Engl J Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0160","doi-asserted-by":"crossref","first-page":"L92","DOI":"10.1152\/ajplung.90388.2008","article-title":"Tracheobronchial air\u2013liquid interface cell culture: a model for innate mucosal defense of the upper airways?","volume":"296","author":"Kesimer","year":"2009","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0165","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.heares.2011.05.003","article-title":"Regulation of sodium transport in the inner ear","volume":"280","author":"Kim","year":"2011","journal-title":"Hear Res"},{"key":"10.1016\/j.biocel.2014.03.002_bib0170","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1172\/JCI0215217","article-title":"Mucus clearance as a primary innate defense mechanism for mammalian airways","volume":"109","author":"Knowles","year":"2002","journal-title":"J Clin Invest"},{"key":"10.1016\/j.biocel.2014.03.002_bib0175","doi-asserted-by":"crossref","first-page":"2588","DOI":"10.1172\/JCI119802","article-title":"Ion composition of airway surface liquid of patients with cystic fibrosis as compared with normal and disease-control subjects","volume":"100","author":"Knowles","year":"1997","journal-title":"J Clin Invest"},{"key":"10.1016\/j.biocel.2014.03.002_bib0180","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1164\/ajrccm.150.2.8049828","article-title":"Bronchoalveolar lavage findings in cystic fibrosis patients with stable, clinically mild lung disease suggest ongoing infection and inflammation","volume":"150","author":"Konstan","year":"1994","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0185","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nm1028","article-title":"Increased airway epithelial Na+ absorption produces cystic fibrosis-like lung disease in mice","volume":"10","author":"Mall","year":"2004","journal-title":"Nat Med"},{"key":"10.1016\/j.biocel.2014.03.002_bib0190","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/S0092-8674(00)81724-9","article-title":"Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease","volume":"95","author":"Matsui","year":"1998","journal-title":"Cell"},{"key":"10.1016\/j.biocel.2014.03.002_bib0195","doi-asserted-by":"crossref","first-page":"e13224","DOI":"10.1371\/journal.pone.0013224","article-title":"The multifunctional host defense peptide SPLUNC1 is critical for homeostasis of the mammalian upper airway","volume":"5","author":"McGillivary","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.biocel.2014.03.002_bib0200","doi-asserted-by":"crossref","first-page":"5340","DOI":"10.1073\/pnas.91.12.5340","article-title":"Bicarbonate conductance and pH regulatory capability of cystic fibrosis transmembrane conductance regulator","volume":"91","author":"Poulsen","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biocel.2014.03.002_bib0205","doi-asserted-by":"crossref","first-page":"255","DOI":"10.4161\/chan.4.4.12255","article-title":"SPLUNC1 expression reduces surface levels of the epithelial sodium channel (ENaC) in Xenopus laevis oocytes","volume":"4","author":"Rollins","year":"2010","journal-title":"Channels (Austin)"},{"key":"10.1016\/j.biocel.2014.03.002_bib0210","article-title":"Activation of the epithelial sodium channel (ENaC) by serine proteases","author":"Rossier","year":"2008","journal-title":"Annu Rev Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0215","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1002\/pmic.200500273","article-title":"Proteomic analysis of nasal cells from cystic fibrosis patients and non-cystic fibrosis control individuals: search for novel biomarkers of cystic fibrosis lung disease","volume":"6","author":"Roxo-Rosa","year":"2006","journal-title":"Proteomics"},{"key":"10.1016\/j.biocel.2014.03.002_bib0220","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1085\/jgp.118.2.223","article-title":"The relative roles of passive surface forces and active ion transport in the modulation of airway surface liquid volume and composition","volume":"118","author":"Tarran","year":"2001","journal-title":"J Gen Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0225","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S1097-2765(01)00286-6","article-title":"The CF salt controversy: in vivo observations and therapeutic approaches","volume":"8","author":"Tarran","year":"2001","journal-title":"Mol Cell"},{"key":"10.1016\/j.biocel.2014.03.002_bib0230","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1085\/jgp.200509468","article-title":"Soluble mediators, not cilia, determine airway surface liquid volume in normal and cystic fibrosis superficial airway epithelia","volume":"127","author":"Tarran","year":"2006","journal-title":"J Gen Physiol"},{"key":"10.1016\/j.biocel.2014.03.002_bib0235","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/0092-8674(93)90353-R","article-title":"Molecular mechanisms of CFTR chloride channel dysfunction in cystic fibrosis","volume":"73","author":"Welsh","year":"1993","journal-title":"Cell"},{"key":"10.1016\/j.biocel.2014.03.002_bib0240","doi-asserted-by":"crossref","first-page":"13698","DOI":"10.1074\/jbc.274.19.13698","article-title":"Differential display identification of plunc, a novel gene expressed in embryonic palate, nasal epithelium, and adult lung","volume":"274","author":"Weston","year":"1999","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2014.03.002_bib0245","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1172\/JCI0213870","article-title":"Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients","volume":"109","author":"Worlitzsch","year":"2002","journal-title":"J Clin Invest"},{"key":"10.1016\/j.biocel.2014.03.002_bib0250","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/gcc.10247","article-title":"Identification of tissue-specific genes in nasopharyngeal epithelial tissue and differentially expressed genes in nasopharyngeal carcinoma by suppression subtractive hybridization and cDNA microarray","volume":"38","author":"Zhang","year":"2003","journal-title":"Genes Chromosomes Cancer"},{"key":"10.1016\/j.biocel.2014.03.002_bib0255","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s11010-007-9659-3","article-title":"Effect of SPLUNC1 protein on the Pseudomonas aeruginosa and Epstein\u2013Barr virus","volume":"309","author":"Zhou","year":"2008","journal-title":"Mol Cell Biochem"}],"container-title":["The International Journal of Biochemistry &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1357272514000752?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1357272514000752?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T06:54:31Z","timestamp":1761202471000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1357272514000752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":51,"alternative-id":["S1357272514000752"],"URL":"https:\/\/doi.org\/10.1016\/j.biocel.2014.03.002","relation":{},"ISSN":["1357-2725"],"issn-type":[{"value":"1357-2725","type":"print"}],"subject":[],"published":{"date-parts":[[2014,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mammalian short palate lung and nasal epithelial clone 1 (SPLUNC1) in pH-dependent airway hydration","name":"articletitle","label":"Article Title"},{"value":"The International Journal of Biochemistry & Cell Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biocel.2014.03.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}