{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:23:02Z","timestamp":1780503782642,"version":"3.54.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004726","name":"Ministry of Health of Russian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cGALLY\u201d International Biomedical Research LLC, San Antonio, Texas, USA"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The International Journal of Biochemistry & Cell Biology"],"published-print":{"date-parts":[[2015,5]]},"DOI":"10.1016\/j.biocel.2015.03.001","type":"journal-article","created":{"date-parts":[[2015,3,9]],"date-time":"2015-03-09T21:15:35Z","timestamp":1425935735000},"page":"115-124","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"special_numbering":"C","title":["Glutenase and collagenase activities of wheat cysteine protease Triticain-\u03b1: Feasibility for enzymatic therapy assays"],"prefix":"10.1016","volume":"62","author":[{"given":"Lyudmila V.","family":"Savvateeva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neonila V.","family":"Gorokhovets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir A.","family":"Makarov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Serebryakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey G.","family":"Solovyev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey Yu.","family":"Morozov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. Prakash","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeni Yu.","family":"Zernii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Andrey A.","family":"Zamyatnin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gjumrakch","family":"Aliev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biocel.2015.03.001_bib0005","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.2174\/0929867321666131227161303","article-title":"Oxidative stress mediated mitochondrial and vascular lesions as markers in the pathogenesis of Alzheimer disease","volume":"21","author":"Aliev","year":"2014","journal-title":"Curr Med Chem"},{"key":"10.1016\/j.biocel.2015.03.001_bib0010","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/B978-0-12-416039-2.00013-6","article-title":"Oral enzyme therapy for celiac sprue","volume":"502","author":"Bethune","year":"2012","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0015","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.chembiol.2006.04.008","article-title":"Heterologous expression, purification, refolding, and structural-functional characterization of EP-B2, a self-activating barley cysteine endoprotease","volume":"13","author":"Bethune","year":"2006","journal-title":"Chem Biol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0020","doi-asserted-by":"crossref","first-page":"14463","DOI":"10.1073\/pnas.0506948102","article-title":"Cysteine protease mcII-Pa executes programmed cell death during plant embryogenesis","volume":"102","author":"Bozhkov","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biocel.2015.03.001_bib0025","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/emboj.2010.1","article-title":"Phytaspase, a relocalisable cell death promoting plant protease with caspase specificity","volume":"29","author":"Chichkova","year":"2010","journal-title":"EMBO J"},{"key":"10.1016\/j.biocel.2015.03.001_bib0030","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1146\/annurev-biochem-061809-121639","article-title":"Caspase substrates and cellular remodeling","volume":"80","author":"Crawford","year":"2011","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.biocel.2015.03.001_bib0035","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1177\/1756287214537331","article-title":"Experience in the use of collagenase Clostridium histolyticum in the management of Peyronie's disease: current data and future prospects","volume":"6","author":"Egui Rojo","year":"2014","journal-title":"Ther Adv Urol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0040","first-page":"501","article-title":"Treatment of celiac disease: from gluten-free diet to novel therapies","volume":"66","author":"Francavilla","year":"2014","journal-title":"Minerva Pediatr"},{"key":"10.1016\/j.biocel.2015.03.001_bib0045","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1053\/j.gastro.2007.05.028","article-title":"Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue","volume":"133","author":"Gass","year":"2007","journal-title":"Gastroenterology"},{"key":"10.1016\/j.biocel.2015.03.001_bib0050","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1124\/jpet.106.104315","article-title":"Effect of barley endoprotease EP-B2 on gluten digestion in the intact rat","volume":"318","author":"Gass","year":"2006","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1016\/j.biocel.2015.03.001_bib0055","doi-asserted-by":"crossref","first-page":"13","DOI":"10.12968\/jowc.1997.6.1.13","article-title":"Crab collagenase in wound debridement","volume":"6","author":"Glyantsev","year":"1997","journal-title":"J Wound Care"},{"key":"10.1016\/j.biocel.2015.03.001_bib0060","doi-asserted-by":"crossref","first-page":"20513","DOI":"10.1021\/ja3094795","article-title":"Computational design of an \u03b1-gliadin peptidase","volume":"134","author":"Gordon","year":"2012","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.biocel.2015.03.001_bib0065","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1346-8138.2009.00738.x","article-title":"Mechanisms of skin fibrosis in systemic sclerosis","volume":"37","author":"Jinnin","year":"2010","journal-title":"J Dermatol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0070","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.1093\/nar\/25.16.3371","article-title":"Rapid and efficient site-directed mutagenesis by single-tube \u2018megaprimer\u2019 PCR method","volume":"25","author":"Ke","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.biocel.2015.03.001_bib0075","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1016\/j.bbagen.2007.08.003","article-title":"Plant collagenase: unique collagenolytic activity of cysteine proteases from ginger","volume":"1770","author":"Kim","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.biocel.2015.03.001_bib0080","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jplph.2008.02.006","article-title":"Wheat cysteine proteases triticain alpha, beta and gamma exhibit mutually distinct responses to gibberellin in germinating seeds","volume":"166","author":"Kiyosaki","year":"2009","journal-title":"J Plant Physiol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0085","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1111\/j.1742-4658.2007.05749.x","article-title":"Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum aestivum L., specifically digests gliadin and is regulated by intrinsic cystatins","volume":"274","author":"Kiyosaki","year":"2007","journal-title":"FEBS J"},{"key":"10.1016\/j.biocel.2015.03.001_bib0090","doi-asserted-by":"crossref","first-page":"112","DOI":"10.2174\/18715273113126660130","article-title":"Pathogenesis of Alzheimer disease: role of oxidative stress, amyloid-\u03b2 peptides, systemic ammonia and erythrocyte energy metabolism","volume":"13","author":"Kosenko","year":"2014","journal-title":"CNS Neurol Disord Drug Targets"},{"key":"10.1016\/j.biocel.2015.03.001_bib0095","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.biocel.2015.03.001_bib0100","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1053\/j.gastro.2014.02.031","article-title":"Glutenase ALV003 attenuates gluten-induced mucosal injury in patients with celiac disease","volume":"146","author":"L\u00e4hdeaho","year":"2014","journal-title":"Gastroenterology"},{"key":"10.1016\/j.biocel.2015.03.001_bib0105","first-page":"10","article-title":"Gastrointestinal absorption and biological activities of serine and cysteine proteases of animal and plant origin: review on absorption of serine and cysteine proteases","volume":"4","author":"Lorkowski","year":"2012","journal-title":"Int J Physiol Pathophysiol Pharmacol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0110","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1097\/MOG.0000000000000041","article-title":"Advances in coeliac disease","volume":"30","author":"Lundin","year":"2014","journal-title":"Curr Opin Gastroenterol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0115","article-title":"Non-surgical treatment of deep wounds triggered by harmful physical and chemical agents: a successful combined use of collagenase and hyaluronic acid","author":"Onesti","year":"2014","journal-title":"Int Wound J"},{"key":"10.1016\/j.biocel.2015.03.001_bib0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1399-3054.2012.01614.x","article-title":"Plant proteases \u2013 from detection to function","volume":"145","author":"Pesquet","year":"2012","journal-title":"Physiol Plant"},{"key":"10.1016\/j.biocel.2015.03.001_bib0125","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1038\/nrg1111","article-title":"Human and mouse proteases: a comparative genomic approach","volume":"4","author":"Puente","year":"2003","journal-title":"Nat Rev Genet"},{"key":"10.1016\/j.biocel.2015.03.001_bib0130","doi-asserted-by":"crossref","first-page":"D239","DOI":"10.1093\/nar\/gkn570","article-title":"The Degradome database: mammalian proteases and diseases of proteolysis","volume":"37","author":"Quesada","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.biocel.2015.03.001_bib0135","doi-asserted-by":"crossref","first-page":"D503","DOI":"10.1093\/nar\/gkt953","article-title":"MEROPS: the database of proteolytic enzymes, their substrates and inhibitors","volume":"42","author":"Rawlings","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.biocel.2015.03.001_bib0140","doi-asserted-by":"crossref","first-page":"4702","DOI":"10.1021\/pr400663t","article-title":"One hundred years of grain omics: identifying the glutens that feed the world","volume":"12","author":"Ribeiro","year":"2013","journal-title":"J Proteome Res"},{"key":"10.1016\/j.biocel.2015.03.001_bib0145","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1104\/pp.112.194001","article-title":"Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics","volume":"158","author":"Richau","year":"2012","journal-title":"Plant Physiol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0150","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.cld.2006.08.017","article-title":"Hepatic fibrosis, stellate cells, and portal hypertension","volume":"10","author":"Rockey","year":"2006","journal-title":"Clin Liver Dis"},{"key":"10.1016\/j.biocel.2015.03.001_bib0155","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1016\/j.phytochem.2008.05.016","article-title":"Plant cysteine proteinases: evaluation of the pharmacological activity","volume":"69","author":"Salas","year":"2008","journal-title":"Phytochemistry"},{"key":"10.1016\/j.biocel.2015.03.001_bib0160","series-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.biocel.2015.03.001_bib0165","doi-asserted-by":"crossref","first-page":"477","DOI":"10.2174\/1381612053382098","article-title":"Fibrosis as a therapeutic target post-myocardial infarction","volume":"11","author":"See","year":"2005","journal-title":"Curr Pharm Des"},{"key":"10.1016\/j.biocel.2015.03.001_bib0170","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1126\/science.1074129","article-title":"Structural basis for gluten intolerance in celiac sprue","volume":"297","author":"Shan","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.biocel.2015.03.001_bib0175","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1093\/jexbot\/53.370.947","article-title":"Cereal seed storage proteins: structures, properties and role in grain utilization","volume":"53","author":"Shewry","year":"2002","journal-title":"J Exp Bot"},{"key":"10.1016\/j.biocel.2015.03.001_bib0180","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.chembiol.2006.04.009","article-title":"Rational design of combination enzyme therapy for celiac sprue","volume":"13","author":"Siegel","year":"2006","journal-title":"Chem Biol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0185","first-page":"e13","article-title":"Treatment of Dupuytren disease with injectable collagenase in a veteran population: a case series at the Department of Veterans Affairs New Jersey Health Care System","volume":"14","author":"Sood","year":"2014","journal-title":"Eplasty"},{"key":"10.1016\/j.biocel.2015.03.001_bib0190","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/S0014-5793(01)02486-3","article-title":"Protein misfolding and disease; protein refolding and therapy","volume":"498","author":"Soto","year":"2001","journal-title":"FEBS Lett"},{"key":"10.1016\/j.biocel.2015.03.001_bib0195","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/j.1365-2249.2010.04119.x","article-title":"Degradation of coeliac disease-inducing rye secalin by germinating cereal enzymes: diminishing toxic effects in intestinal epithelial cells","volume":"161","author":"Stenman","year":"2010","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0200","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/ncb1979","article-title":"Tudor staphylococcal nuclease is an evolutionarily conserved component of the programmed cell death degradome","volume":"11","author":"Sundstr\u00f6m","year":"2009","journal-title":"Nat Cell Biol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0205","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.clinthera.2013.09.013","article-title":"Clinical and economic assessment of diabetic foot ulcer debridement with collagenase: results of a randomized controlled study","volume":"35","author":"Tallis","year":"2013","journal-title":"Clin Ther"},{"key":"10.1016\/j.biocel.2015.03.001_bib0210","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.jplph.2006.02.009","article-title":"Differential expression of wheat aspartic proteinases, WAP1 and WAP2, in germinating and maturing seeds","volume":"164","author":"Tamura","year":"2007","journal-title":"J Plant Physiol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0215","doi-asserted-by":"crossref","first-page":"G769","DOI":"10.1152\/ajpgi.00144.2014","article-title":"Effect of Rothia mucilaginosa enzymes on gliadin (gluten) structure, deamidation, and immunogenic epitopes relevant to celiac disease","volume":"307","author":"Tian","year":"2014","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0220","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1146\/annurev.arplant.59.032607.092835","article-title":"Plant proteases: from phenotypes to molecular mechanisms","volume":"59","author":"van der Hoorn","year":"2008","journal-title":"Annu Rev Plant Biol"},{"key":"10.1016\/j.biocel.2015.03.001_bib0225","doi-asserted-by":"crossref","first-page":"45329","DOI":"10.1074\/jbc.M406329200","article-title":"Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave substrates after arginine and lysine","volume":"279","author":"Vercammen","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biocel.2015.03.001_bib0230","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1094\/CCHEM-85-1-0001","article-title":"The biochemical basis of celiac disease","volume":"85","author":"Wieser","year":"2008","journal-title":"Cereal Chem"},{"key":"10.1016\/j.biocel.2015.03.001_bib0235","doi-asserted-by":"crossref","first-page":"356","DOI":"10.5740\/jaoacint.SGE_Wieser","article-title":"Detoxification of gluten by means of enzymatic treatment","volume":"95","author":"Wieser","year":"2012","journal-title":"J AOAC Int"},{"key":"10.1016\/j.biocel.2015.03.001_bib0240","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1007\/s00726-012-1444-2","article-title":"Dietary requirements of \u201cnutritionally non-essential amino acids\u201d by animals and humans","volume":"44","author":"Wu","year":"2013","journal-title":"Amino Acids"}],"container-title":["The International Journal of Biochemistry & Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1357272515000709?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1357272515000709?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T06:58:21Z","timestamp":1761202701000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1357272515000709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5]]},"references-count":48,"alternative-id":["S1357272515000709"],"URL":"https:\/\/doi.org\/10.1016\/j.biocel.2015.03.001","relation":{},"ISSN":["1357-2725"],"issn-type":[{"value":"1357-2725","type":"print"}],"subject":[],"published":{"date-parts":[[2015,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Glutenase and collagenase activities of wheat cysteine protease Triticain-\u03b1: Feasibility for enzymatic therapy assays","name":"articletitle","label":"Article Title"},{"value":"The International Journal of Biochemistry & Cell Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biocel.2015.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}