{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:32:41Z","timestamp":1789475561139,"version":"build-2803163510"},"reference-count":42,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100007397","name":"Charles University in Prague","doi-asserted-by":"crossref","award":["53707 B"],"award-info":[{"award-number":["53707 B"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007397","name":"Charles University in Prague","doi-asserted-by":"crossref","award":["SVV 265 003\/2012"],"award-info":[{"award-number":["SVV 265 003\/2012"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimie"],"published-print":{"date-parts":[[2012,12]]},"DOI":"10.1016\/j.biochi.2012.09.006","type":"journal-article","created":{"date-parts":[[2012,9,17]],"date-time":"2012-09-17T13:47:45Z","timestamp":1347889665000},"page":"2681-2686","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["In\u00a0vitro platelet antiaggregatory properties of 4-methylcoumarins"],"prefix":"10.1016","volume":"94","author":[{"given":"Kate\u0159ina","family":"Mac\u00e1kov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"\u0158eh\u00e1kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u0159emysl","family":"Mlad\u011bnka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Karl\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1\u0161","family":"Filipsk\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"\u0158\u00edha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virinder S.","family":"Parmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lud\u011bk","family":"Jahod\u00e1\u0159","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"P\u00e1vek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radom\u00edr","family":"Hrdina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Saso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biochi.2012.09.006_bib1","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1016\/0002-9149(74)90712-7","article-title":"Editorial: pathogenesis of myocardial infarction (culpable clot?)","volume":"34","author":"Burchell","year":"1974","journal-title":"Am. J. Cardiol."},{"key":"10.1016\/j.biochi.2012.09.006_bib2","doi-asserted-by":"crossref","first-page":"887","DOI":"10.2165\/11536000-000000000-00000","article-title":"Antiplatelet drugs\u2013do we need new options? with a reappraisal of direct thromboxane inhibitors","volume":"70","author":"Coccheri","year":"2010","journal-title":"Drugs"},{"key":"10.1016\/j.biochi.2012.09.006_bib3","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1056\/NEJMoa050613","article-title":"A\u00a0randomized trial of low-dose aspirin in the primary prevention of cardiovascular disease in women","volume":"352","author":"Ridker","year":"2005","journal-title":"New Engl. J. Med."},{"key":"10.1016\/j.biochi.2012.09.006_bib4","doi-asserted-by":"crossref","first-page":"27A","DOI":"10.1016\/j.amjcard.2008.11.020","article-title":"Variability in responsiveness to oral antiplatelet therapy","volume":"103","author":"Angiolillo","year":"2009","journal-title":"Am. J. Cardiol."},{"key":"10.1016\/j.biochi.2012.09.006_bib5","doi-asserted-by":"crossref","first-page":"2202","DOI":"10.1093\/eurheartj\/ehn287","article-title":"A\u00a0randomized study assessing the impact of cilostazol on platelet function profiles in patients with diabetes mellitus and coronary artery disease on dual antiplatelet therapy: results of the OPTIMUS-2 study","volume":"29","author":"Angiolillo","year":"2008","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.biochi.2012.09.006_bib6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1002\/hlca.19960790308","article-title":"Synthesis of coumarin derivatives as inhibitors of platelet aggregation","volume":"79","author":"Chen","year":"1996","journal-title":"Helv. Chim. Acta"},{"key":"10.1016\/j.biochi.2012.09.006_bib7","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.bcp.2003.10.009","article-title":"Mechanisms involved in the antiplatelet activity of 8-methyl-4-(1-piperazinyl)-7-(3-pyridinylmethoxy)-2H-1-benzopyran-2-one (RC414)","volume":"67","author":"Leoncini","year":"2004","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib8","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.freeradbiomed.2010.06.010","article-title":"Cardiovascular effects of flavonoids are not caused only by direct antioxidant activity","volume":"49","author":"Mladenka","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.biochi.2012.09.006_bib9","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1002\/hlca.19960790612","article-title":"Antiplatelet \u03b1-methylidene-\u03b3-butyrolactones: synthesis and evaluation of quinoline, flavone, and xanthone derivatives","volume":"79","author":"Wang","year":"1996","journal-title":"Helv Chim. Acta"},{"key":"10.1016\/j.biochi.2012.09.006_bib10","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1211\/jpp.59.12.0015","article-title":"Antioxidant activity of 4-methylcoumarins","volume":"59","author":"Pedersen","year":"2007","journal-title":"J.\u00a0Pharm. Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib11","first-page":"132","article-title":"Antioxidant drugs for treating beta-cell oxidative stress in type 2 diabetes: glucose-centric versus insulin-centric therapy","volume":"9","author":"Robertson","year":"2010","journal-title":"Discov. Med."},{"key":"10.1016\/j.biochi.2012.09.006_bib12","first-page":"RA209","article-title":"Oxidative stress and ischemic myocardial syndromes","volume":"15","author":"Misra","year":"2009","journal-title":"Med. Sci. Monit."},{"key":"10.1016\/j.biochi.2012.09.006_bib13","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1080\/14756360701674421","article-title":"Evaluation of natural substances from Evolvulus alsinoides L. with the purpose of determining their antioxidant potency","volume":"23","author":"Cervenka","year":"2008","journal-title":"J.\u00a0Enzym. Inhib. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib14","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1002\/mrc.1260260515","article-title":"Carbon-13 nuclear magnetic resonance spectroscopy of 4-methylcoumarins (4-methyl-2H-1-benzopyran-2-ones)","volume":"26","author":"Parmar","year":"1988","journal-title":"Mag Res. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib15","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1136\/jcp.15.5.452","article-title":"Platelet aggregation: part II some results from a new method of study","volume":"15","author":"O'Brien","year":"1962","journal-title":"J.\u00a0Clin. Pathol."},{"key":"10.1016\/j.biochi.2012.09.006_bib16","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1038\/194927b0","article-title":"Aggregation of blood platelets by adenosine diphosphate and its reversal","volume":"194","author":"Born","year":"1962","journal-title":"Nature"},{"key":"10.1016\/j.biochi.2012.09.006_bib17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0006-2952(97)00179-2","article-title":"Thromboxane A2 synthase inhibition and thromboxane A2 receptor blockade by 2-[(4-cyanophenyl)amino]-3-chloro-1,4-naphthalenedione (NQ-Y15) in rat platelets","volume":"54","author":"Chang","year":"1997","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib18","series-title":"Thromboxane B2 EIA Kit","author":"Cayman Chemical Company","year":"1942012"},{"key":"10.1016\/j.biochi.2012.09.006_bib19","series-title":"COX Inhibitor Screening Assay Kit","author":"Cayman Chemical Company","year":"162012"},{"key":"10.1016\/j.biochi.2012.09.006_bib20","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0049-3848(91)90208-E","article-title":"Inhibition of platelet aggregation by some flavonoids","volume":"64","author":"Tzeng","year":"1991","journal-title":"Thromb. Res."},{"key":"10.1016\/j.biochi.2012.09.006_bib21","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1080\/14756360500323022","article-title":"Antiinflammatory and antioxidant evaluation of novel coumarin derivatives","volume":"21","author":"Kontogiorgis","year":"2006","journal-title":"J.\u00a0Enzym. Inhib. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib22","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.ejmech.2006.03.007","article-title":"Synthesis, structure, toxicological and pharmacological investigations of 4-hydroxycoumarin derivatives","volume":"41","author":"Manolov","year":"2006","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib23","doi-asserted-by":"crossref","first-page":"887","DOI":"10.2174\/0929867053507315","article-title":"Simple coumarins and analogues in medicinal chemistry: occurrence, synthesis and biological activity","volume":"12","author":"Borges","year":"2005","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib24","doi-asserted-by":"crossref","first-page":"365","DOI":"10.2174\/138955706776361457","article-title":"Synthetic and natural coumarins as antioxidants","volume":"6","author":"Kostova","year":"2006","journal-title":"Mini Rev. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib25","doi-asserted-by":"crossref","first-page":"3813","DOI":"10.2174\/1381612043382710","article-title":"Natural and synthetic coumarin derivatives with anti-inflammatory\/antioxidant activities","volume":"10","author":"Fylaktakidou","year":"2004","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.biochi.2012.09.006_bib26","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/0306-3623(95)02112-4","article-title":"Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential","volume":"27","author":"Hoult","year":"1996","journal-title":"Gen. Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib27","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/0031-9422(95)00054-B","article-title":"Coumarins and anti-platelet aggregation constituents from Zanthoxylum schinifolium","volume":"39","author":"Chen","year":"1995","journal-title":"Phytochemistry"},{"key":"10.1016\/j.biochi.2012.09.006_bib28","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.bmcl.2005.10.013","article-title":"Design, synthesis, and vasorelaxant and platelet antiaggregatory activities of coumarin-resveratrol hybrids","volume":"16","author":"Vilar","year":"2006","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.biochi.2012.09.006_bib29","doi-asserted-by":"crossref","first-page":"122","DOI":"10.3390\/molecules13010122","article-title":"Antiplatelet aggregation coumarins from the leaves of Murraya omphalocarpa","volume":"13","author":"Chia","year":"2008","journal-title":"Molecules"},{"key":"10.1016\/j.biochi.2012.09.006_bib30","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/S0031-9422(97)00678-X","article-title":"Anti-platelet aggregation constituents from formosan Toddalia asiatica","volume":"48","author":"Tsai","year":"1998","journal-title":"Phytochemistry"},{"key":"10.1016\/j.biochi.2012.09.006_bib31","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0968-0896(02)00307-3","article-title":"Coumarin, chromone, and 4(3H)-pyrimidinone novel bicyclic and tricyclic derivatives as antiplatelet agents: synthesis, biological evaluation, and comparative molecular field analysis","volume":"11","author":"Roma","year":"2003","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib32","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0049-3848(92)90309-X","article-title":"Antiplatelet actions of some coumarin compounds isolated from plant sources","volume":"66","author":"Teng","year":"1992","journal-title":"Thromb. Res."},{"key":"10.1016\/j.biochi.2012.09.006_bib33","first-page":"521","article-title":"Trigocoumarin \u2013 a new coumarin from Trigonella foenumgraecum","volume":"37B","author":"Parmar","year":"1981","journal-title":"Z.\u00a0Naturforsch."},{"key":"10.1016\/j.biochi.2012.09.006_bib34","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/S0031-9422(00)84916-X","article-title":"Troupin, a 4-methylcoumarin from Tamarix troupii","volume":"24","author":"Parmar","year":"1985","journal-title":"Phytochemistry"},{"key":"10.1016\/j.biochi.2012.09.006_bib35","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0300-483X(94)90114-7","article-title":"Comparison of in\u00a0vivo and in\u00a0vitro rat hepatic toxicity of coumarin and methyl analogues, and application of quantitative morphometry to toxicity in\u00a0vivo","volume":"88","author":"Fernyhough","year":"1994","journal-title":"Toxicology"},{"key":"10.1016\/j.biochi.2012.09.006_bib36","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0041-008X(89)90336-0","article-title":"Studies on the mechanism of coumarin-induced toxicity in rat hepatocytes: comparison with dihydrocoumarin and other coumarin metabolites","volume":"97","author":"Lake","year":"1989","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib37","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0887-2333(92)90081-2","article-title":"Toxicity of coumarin and various methyl derivatives in cultures of rat hepatocytes and V79 cells","volume":"6","author":"Fentema","year":"1992","journal-title":"Toxicol. In\u00a0Vitro"},{"key":"10.1016\/j.biochi.2012.09.006_bib38","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.ejmech.2003.12.010","article-title":"Synthesis and in\u00a0vitro inhibitory activity on human platelet aggregation of novel properly substituted 4-(1-piperazinyl)coumarins","volume":"39","author":"Di Braccio","year":"2004","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib39","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.thromres.2007.10.005","article-title":"Platelet collagen receptors, signaling and antagonism: emerging approaches for the prevention of intravascular thrombosis","volume":"122","author":"Surin","year":"2008","journal-title":"Thromb. Res."},{"key":"10.1016\/j.biochi.2012.09.006_bib40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.vph.2007.12.004","article-title":"Dipyridamole, cerebrovascular disease, and the vasculature","volume":"48","author":"Chakrabarti","year":"2008","journal-title":"Vascul Pharmacol."},{"key":"10.1016\/j.biochi.2012.09.006_bib41","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.1021\/jm0611511","volume":"50","author":"Roma","year":"2007","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.biochi.2012.09.006_bib42","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1016\/j.biochi.2010.06.011","article-title":"Specificity of calreticulin transacetylase to acetoxy derivatives of benzofurans: effect on the activation of platelet nitric oxide synthase","volume":"92","author":"Gupta","year":"2010","journal-title":"Biochimie"}],"container-title":["Biochimie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908412003641?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908412003641?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:07:54Z","timestamp":1759540074000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300908412003641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["S0300908412003641"],"URL":"https:\/\/doi.org\/10.1016\/j.biochi.2012.09.006","relation":{},"ISSN":["0300-9084"],"issn-type":[{"value":"0300-9084","type":"print"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0vitro platelet antiaggregatory properties of 4-methylcoumarins","name":"articletitle","label":"Article Title"},{"value":"Biochimie","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biochi.2012.09.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier Masson SAS. All rights reserved.","name":"copyright","label":"Copyright"}]}}