{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T13:00:53Z","timestamp":1768914053142,"version":"3.49.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"EU Biomed II","award":["DMH-CT94-1708"],"award-info":[{"award-number":["DMH-CT94-1708"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimie"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.biochi.2020.03.001","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T00:45:59Z","timestamp":1583714759000},"page":"107-113","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Body mass index determines the response of plasma sulfur amino acids to methionine loading"],"prefix":"10.1016","volume":"173","author":[{"given":"Amany K.","family":"Elshorbagy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga","family":"Refsum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.biochi.2020.03.001_bib1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1093\/ajcn\/88.3.738","article-title":"Homocysteine, cysteine, and body composition in the Hordaland Homocysteine Study: does cysteine link amino acid and lipid metabolism?","volume":"88","author":"Elshorbagy","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.biochi.2020.03.001_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0044166","article-title":"The association of cysteine with obesity, inflammatory cytokines and insulin resistance in hispanic children and adolescents","volume":"7","author":"Elshorbagy","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.biochi.2020.03.001_bib3","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.jnutbio.2010.12.009","article-title":"Dietary cystine level affects metabolic rate and glycaemic control in adult mice","volume":"23","author":"Elshorbagy","year":"2012","journal-title":"J.\u00a0Nutr. Biochem."},{"key":"10.1016\/j.biochi.2020.03.001_bib4","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.nut.2009.09.017","article-title":"Sulfur amino acids in methionine-restricted rats: hyperhomocysteinemia","volume":"26","author":"Elshorbagy","year":"2010","journal-title":"Nutrition"},{"key":"10.1016\/j.biochi.2020.03.001_bib5","doi-asserted-by":"crossref","first-page":"R728","DOI":"10.1152\/ajpregu.00837.2009","article-title":"Dietary methionine restriction enhances metabolic flexibility and increases uncoupled respiration in both fed and fasted states","volume":"299","author":"Hasek","year":"2010","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"10.1016\/j.biochi.2020.03.001_bib6","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1096\/fj.201701211R","article-title":"Short-term methionine deprivation improves metabolic health via sexually dimorphic, mTORC1-independent mechanisms","volume":"32","author":"Yu","year":"2018","journal-title":"Faseb. J."},{"key":"10.1016\/j.biochi.2020.03.001_bib7","doi-asserted-by":"crossref","first-page":"E836","DOI":"10.1210\/jc.2010-2493","article-title":"Dietary methionine restriction increases fat oxidation in obese adults with metabolic syndrome","volume":"96","author":"Plaisance","year":"2011","journal-title":"J.\u00a0Clin. Endocrinol. Metab."},{"key":"10.1016\/j.biochi.2020.03.001_bib8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1194\/jlr.M010215","article-title":"Cysteine supplementation reverses methionine restriction effects on rat adiposity: significance of stearoyl-coenzyme A desaturase","volume":"52","author":"Elshorbagy","year":"2011","journal-title":"J.\u00a0Lipid Res."},{"key":"10.1016\/j.biochi.2020.03.001_bib9","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s10863-015-9608-x","article-title":"Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction","volume":"47","author":"Gomez","year":"2015","journal-title":"J.\u00a0Bioenerg. Biomembr."},{"key":"10.1016\/j.biochi.2020.03.001_bib10","first-page":"623","article-title":"Cysteine deprivation prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells","volume":"1862","author":"Haj-Yasein","year":"2017","journal-title":"Biochim Biophys Acta BBA - Mol Cell Biol Lipids"},{"key":"10.1016\/j.biochi.2020.03.001_bib11","first-page":"49","article-title":"Cysteine and obesity: consistency of the evidence across epidemiologic, animal and cellular studies","volume":"15","author":"Elshorbagy","year":"2012","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"10.1016\/j.biochi.2020.03.001_bib12","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.numecd.2011.01.008","article-title":"The association of fasting plasma sulfur-containing compounds with BMI, serum lipids and apolipoproteins","volume":"22","author":"Elshorbagy","year":"2012","journal-title":"Nutr. Metabol. Cardiovasc. Dis."},{"key":"10.1016\/j.biochi.2020.03.001_bib13","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1007\/s00394-016-1237-6","article-title":"Amino acid changes during transition to a vegan diet supplemented with fish in healthy humans","volume":"56","author":"Elshorbagy","year":"2017","journal-title":"Eur. J. Nutr."},{"key":"10.1016\/j.biochi.2020.03.001_bib14","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.3390\/nu10121822","article-title":"Combining dietary sulfur amino acid restriction with polyunsaturated fatty acid intake in humans: a randomized controlled pilot trial","volume":"10","author":"Olsen","year":"2018","journal-title":"Nutrients"},{"key":"10.1016\/j.biochi.2020.03.001_bib15","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1093\/jn\/nxy062","article-title":"Food overconsumption in healthy adults triggers early and sustained increases in serum branched-chain amino acids and changes in cysteine linked to fat gain","volume":"148","author":"Elshorbagy","year":"2018","journal-title":"J.\u00a0Nutr."},{"key":"10.1016\/j.biochi.2020.03.001_bib16","doi-asserted-by":"crossref","first-page":"23P","DOI":"10.1042\/bj1220023Pb","article-title":"The detection of heterozygotes for homocystinuria by oral loading with L-methionine","volume":"122","author":"Fowler","year":"1971","journal-title":"Biochem. J."},{"key":"10.1016\/j.biochi.2020.03.001_bib17","first-page":"67","article-title":"Methionine loading test is necessary for detection of hyperhomocysteinemia","volume":"132","author":"Van Der Griend","year":"1998","journal-title":"J.\u00a0Lab. Clin. Med."},{"key":"10.1016\/j.biochi.2020.03.001_bib18","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1445-5994.2007.01596.x","article-title":"Methionine loading does not enhance the predictive value of homocysteine serum testing for all-cause mortality or major adverse cardiac events","volume":"39","author":"Dunkelgrun","year":"2009","journal-title":"Intern. Med. J."},{"key":"10.1016\/j.biochi.2020.03.001_bib19","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1160\/TH05-07-0498","article-title":"No added value of the methionine loading task in assessment for venous thrombosis and cardiovascular disease risk","volume":"95","author":"Keijzer","year":"2006","journal-title":"Thromb. Haemostasis"},{"key":"10.1016\/j.biochi.2020.03.001_bib20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1096-7192(03)00079-9","article-title":"Genetic variants of homocysteine metabolizing enzymes and the risk of coronary artery disease","volume":"79","author":"Janos\u00edkov\u00e1","year":"2003","journal-title":"Mol. Genet. Metabol."},{"key":"10.1016\/j.biochi.2020.03.001_bib21","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1093\/clinchem\/38.7.1316","article-title":"Dynamic relation between reduced, oxidized, and protein-bound homocysteine and other thiol components in plasma during methionine loading in healthy men","volume":"38","author":"Mansoor","year":"1992","journal-title":"Clin. Chem."},{"key":"10.1016\/j.biochi.2020.03.001_bib22","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1001\/jama.1997.03540460039030","article-title":"Plasma homocysteine as a risk factor for vascular disease. The European Concerted Action Project","volume":"277","author":"Graham","year":"1997","journal-title":"J.\u00a0Am. Med. Assoc."},{"key":"10.1016\/j.biochi.2020.03.001_bib23","series-title":"Homocysteinemia and Vascular Disease: Proceedings of an EC Comac- Epidemiology Expert Group Workshop","first-page":"61","article-title":"Refinement of the methionine loading test","author":"Boers","year":"1990"},{"key":"10.1016\/j.biochi.2020.03.001_bib24","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/clinchem\/39.2.263","article-title":"Homocysteine and other thiols in plasma and urine: automated determination and sample stability","volume":"39","author":"Fiskerstrand","year":"1993","journal-title":"Clin. Chem."},{"key":"10.1016\/j.biochi.2020.03.001_bib25","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1161\/hh1401.093459","article-title":"Investigation of relationship between reduced, oxidized, and protein-bound homocysteine and vascular endothelial function in healthy human subjects","volume":"89","author":"Chambers","year":"2001","journal-title":"Circ. Res."},{"key":"10.1016\/j.biochi.2020.03.001_bib26","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/sj.ejcn.1601755","article-title":"Plasma taurine and cysteine levels following an oral methionine load: relationship with coronary heart disease","volume":"58","author":"Obeid","year":"2004","journal-title":"Eur. J. Clin. Nutr."},{"key":"10.1016\/j.biochi.2020.03.001_bib27","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/ejcn.2015.155","article-title":"Effect of multi-nutrient insufficiency on markers of one carbon metabolism in young women: response to a methionine load","volume":"70","author":"Katre","year":"2016","journal-title":"Eur. J. Clin. Nutr."},{"key":"10.1016\/j.biochi.2020.03.001_bib28","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1093\/clinchem\/38.7.1316","article-title":"Dynamic relation between reduced, oxidized, and protein-bound homocysteine and other thiol components in plasma during methionine loading in healthy men","volume":"38","author":"Mansoor","year":"1992","journal-title":"Clin. Chem."},{"key":"10.1016\/j.biochi.2020.03.001_bib29","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s00726-016-2207-2","article-title":"Homocysteinemia control by cysteine in cerebral vascular patients after methionine loading test: evidences in physiological and pathological conditions in cerebro-vascular and multiple sclerosis patients","volume":"48","author":"Ulivelli","year":"2016","journal-title":"Amino Acids"},{"key":"10.1016\/j.biochi.2020.03.001_bib30","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1053\/meta.2000.16554","article-title":"Synthesis of glutathione in response to methionine load in control subjects and in patients with cirrhosis","volume":"49","author":"Bianchi","year":"2000","journal-title":"Metab. Clin. Exp."},{"key":"10.1016\/j.biochi.2020.03.001_bib31","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1093\/ndt\/16.1.102","article-title":"Effects of methionine loading on plasma and erythrocyte sulphur amino acids and sulph-hydryls before and after co-factor supplementation in haemodialysis patients","volume":"16","author":"Suliman","year":"2001","journal-title":"Nephrol. Dial. Transplant."},{"key":"10.1016\/j.biochi.2020.03.001_bib32","series-title":"Red Cell Membrane Transport in Health and Disease [Internet]","article-title":"Amino acid transport","author":"Ellory","year":"2003"},{"key":"10.1016\/j.biochi.2020.03.001_bib33","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1039\/C9MT00148D","article-title":"Obesity is associated with copper elevation in serum and tissues","volume":"11","author":"Yang","year":"2019","journal-title":"Metall"},{"key":"10.1016\/j.biochi.2020.03.001_bib34","author":"Fan"},{"key":"10.1016\/j.biochi.2020.03.001_bib35","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1007\/s12291-016-0555-z","article-title":"Effect of copper on l-Cysteine\/l-Cystine influx in normal human erythrocytes and erythrocytes of wilson\u2019s disease","volume":"31","author":"Mandal","year":"2016","journal-title":"Indian J. Clin. Biochem."},{"key":"10.1016\/j.biochi.2020.03.001_bib36","series-title":"Advances in Cancer Research [Internet]","article-title":"Chapter three - gamma-glutamyl transpeptidase: redox regulation and drug resistance","author":"Hanigan","year":"2014"},{"key":"10.1016\/j.biochi.2020.03.001_bib37","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.cca.2017.11.026","article-title":"Gamma-glutamyl transferase and the risk of atherosclerosis and coronary heart disease","volume":"476","author":"Ndrepepa","year":"2018","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.biochi.2020.03.001_bib38","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1038\/oby.2008.671","article-title":"The association of plasma cysteine and gamma-glutamyltransferase with BMI and obesity","volume":"17","author":"Elshorbagy","year":"2009","journal-title":"Obes Silver Spring Md"},{"key":"10.1016\/j.biochi.2020.03.001_bib39","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1042\/CS20180157","article-title":"Implications of plasma thiol redox in disease","volume":"132","author":"Oliveira","year":"2018","journal-title":"Clin. Sci."},{"issue":"Supplement 1","key":"10.1016\/j.biochi.2020.03.001_bib40","article-title":"Higher intake of sulfur amino acids is associated with greater mortality among US adults: NHANESIII cohort study","volume":"3","author":"Dong","year":"2019","journal-title":"Curr Dev Nutr"},{"key":"10.1016\/j.biochi.2020.03.001_bib41","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1096\/fasebj.31.1_supplement.966.25","article-title":"The association between dietary sulfur amino acid intake and markers of vascular health","volume":"31","author":"Tran","year":"2017","journal-title":"Faseb. J."},{"key":"10.1016\/j.biochi.2020.03.001_bib42","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0196970","article-title":"Evaluation of tracer labelled methionine load test in vitamin B-12 deficient adolescent women","volume":"13","author":"Bhat","year":"2018","journal-title":"PloS One"}],"container-title":["Biochimie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908420300468?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908420300468?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:13:11Z","timestamp":1759540391000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300908420300468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":42,"alternative-id":["S0300908420300468"],"URL":"https:\/\/doi.org\/10.1016\/j.biochi.2020.03.001","relation":{},"ISSN":["0300-9084"],"issn-type":[{"value":"0300-9084","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Body mass index determines the response of plasma sulfur amino acids to methionine loading","name":"articletitle","label":"Article Title"},{"value":"Biochimie","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biochi.2020.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. and Soci\u00e9t\u00e9 Fran\u00e7aise de Biochimie et Biologie Mol\u00e9culaire (SFBBM). All rights reserved.","name":"copyright","label":"Copyright"}]}}