{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T03:30:33Z","timestamp":1793503833733,"version":"4.5.0"},"reference-count":81,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimie"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1016\/j.biochi.2022.11.006","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T11:22:41Z","timestamp":1668684161000},"page":"1-10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Structural basis of the myotoxic inhibition of the Bothrops pirajai PrTX-I by the synthetic varespladib"],"prefix":"10.1016","volume":"207","author":[{"given":"Guilherme H.M.","family":"Salvador","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00camylle K.R.","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula L.","family":"Ortolani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo L.","family":"Fortes-Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter L.G.","family":"Cavalcante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Lomonte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Lewin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4634-6221","authenticated-orcid":false,"given":"Marcos R.M.","family":"Fontes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biochi.2022.11.006_bib1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"Warrell","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.biochi.2022.11.006_bib2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.toxicon.2003.11.005","article-title":"Skeletal muscle degeneration induced by venom phospholipases A2: insights into the mechanisms of local and systemic myotoxicity","volume":"42","author":"Gutierrez","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib3","series-title":"Envenenamientos por mordeduras de serpientes en Am\u00e9rica Latina y el Caribe: Una visi\u00f3n integral de car\u00e1cter regional","first-page":"L1","author":"Gutierrez","year":"2011"},{"key":"10.1016\/j.biochi.2022.11.006_bib4","series-title":"Neglected Tropical Diseases","author":"World Health Organization","year":"2018"},{"key":"10.1016\/j.biochi.2022.11.006_bib5","doi-asserted-by":"crossref","first-page":"6326","DOI":"10.11609\/JoTT.o4023.6326-34","article-title":"Biology and conservation status of piraja's Lancehead snake Bothrops pirajai amaral, 1923 (Serpentes: viperidae), Brazil","volume":"6","author":"Freitas","year":"2014","journal-title":"J.\u00a0Threat. Taxa"},{"key":"10.1016\/j.biochi.2022.11.006_bib6","series-title":"Atlas dos Remanescentes Florestais da Mata Atl\u00e2ntica Per\u00edodo 2011-2012","first-page":"61","author":"Sosma\/Inpe","year":"2013"},{"key":"10.1016\/j.biochi.2022.11.006_bib7","series-title":"The IUCN Red List of Threatened Species 2021","author":"Silveira","year":"2021"},{"key":"10.1016\/j.biochi.2022.11.006_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxcx.2020.100044","article-title":"Comparative characterization of Viperidae snake venoms from Peru reveals two compositional patterns of phospholipase A2 expression","volume":"7","author":"Lomonte","year":"2020","journal-title":"Toxicon X"},{"key":"10.1016\/j.biochi.2022.11.006_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0109846","article-title":"Synergism between basic Asp49 and Lys49 phospholipase A2 myotoxins of viperid snake venom in\u00a0vitro and in\u00a0vivo","volume":"9","author":"Mora-Obando","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.biochi.2022.11.006_bib10","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/0041-0101(96)00036-0","article-title":"Phospholipase A2 - a structural review","volume":"34","author":"Arni","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib11","doi-asserted-by":"crossref","first-page":"887","DOI":"10.2174\/092986609788923310","article-title":"The intriguing phospholipases A2 homologues: relevant structural features on myotoxicity and catalytic inactivity","volume":"16","author":"dos Santos","year":"2009","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.biochi.2022.11.006_bib12","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jsb.2010.03.019","article-title":"Comparison between apo and complexed structures of bothropstoxin-I reveals the role of Lys122 and Ca2+-binding loop region for the catalytically inactive Lys49-PLA2s","volume":"171","author":"Fernandes","year":"2010","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.biochi.2022.11.006_bib13","series-title":"Handbook of Venoms and Toxins of Reptiles","first-page":"680","article-title":"Snake venom phospholipase A2 toxins","author":"Lomonte","year":"2021"},{"key":"10.1016\/j.biochi.2022.11.006_bib14","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jsb.2009.04.003","article-title":"Comparative structural studies on Lys49-phospholipases A2 from Bothrops genus reveal their myotoxic site","volume":"167","author":"dos Santos","year":"2009","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.biochi.2022.11.006_bib15","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1016\/j.bbapap.2014.09.015","article-title":"A\u00a0structure-based proposal for a comprehensive myotoxic mechanism of phospholipase A2-like proteins from viperid snake venoms","volume":"1844","author":"Fernandes","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biochi.2022.11.006_bib16","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1107\/S1399004715014443","article-title":"Structural and functional evidence for membrane docking and disruption sites on phospholipase A2-like proteins revealed by complexation with the inhibitor suramin","volume":"71","author":"Salvador","year":"2015","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"10.1016\/j.biochi.2022.11.006_bib17","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-15614-z","article-title":"PLA2-like proteins myotoxic mechanism: a dynamic model description","volume":"7","author":"Borges","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biochi.2022.11.006_bib18","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1016\/j.bbamem.2007.01.023","article-title":"Mapping of the structural determinants of artificial and biological membrane damaging activities of a Lys49 phospholipase A2 by scanning alanine mutagenesis","volume":"1768","author":"Chioato","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biochi.2022.11.006_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-73134-9","article-title":"The allosteric activation mechanism of a phospholipase A2-like toxin from Bothrops jararacussu venom: a dynamic description","volume":"10","author":"Gomes","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biochi.2022.11.006_bib20","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.toxicon.2022.02.024","article-title":"Solving the microheterogeneity of Bothrops asper myotoxin-II by high-resolution mass spectrometry: insights into C-terminal region variability in Lys49-phospholipase A2 homologs","volume":"210","author":"Lomonte","year":"2022","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib21","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0041-0101(95)00012-B","article-title":"Fractionation of Bothrops pirajai snake venom: isolation and characterization of piratoxin-I, a new myotoxic protein","volume":"33","author":"Mancuso","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.etp.2005.09.004","article-title":"Paralyzing and myotoxic effects of a recombinant bothropstoxin-I (BthTX-I) on mouse neuromuscular preparations","volume":"57","author":"Gallacci","year":"2006","journal-title":"Exp. Toxicol. Pathol."},{"key":"10.1016\/j.biochi.2022.11.006_bib23","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1590\/S0100-879X2002000600013","article-title":"Effect of suramin on myotoxicity of some crotalid snake venoms","volume":"35","author":"Arruda","year":"2002","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.biochi.2022.11.006_bib24","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/153045","article-title":"Snake venom PLA2s inhibitors isolated from Brazilian plants: synthetic and natural molecules","volume":"2013","author":"Carvalho","year":"2013","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.biochi.2022.11.006_bib25","doi-asserted-by":"crossref","DOI":"10.3390\/toxins12020131","article-title":"Varespladib (LY315920) and methyl varespladib (LY333013) abrogate or delay lethality induced by presynaptically acting neurotoxic snake venoms","volume":"12","author":"Gutierrez","year":"2020","journal-title":"Toxins"},{"key":"10.1016\/j.biochi.2022.11.006_bib26","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.2174\/1389557511313090009","article-title":"Phospholipase A2 inhibitors isolated from medicinal plants: alternative treatment against snakebites","volume":"13","author":"Hage-Melim","year":"2013","journal-title":"Mini Rev. Med. Chem."},{"key":"10.1016\/j.biochi.2022.11.006_bib27","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.toxicon.2009.03.015","article-title":"Neutralization of Bothrops asper venom by antibodies, natural products and synthetic drugs: contributions to understanding snakebite envenomings and their treatment","volume":"54","author":"Lomonte","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib28","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0742-8413(86)90183-0","article-title":"Pharmacological activities of a toxic phospholipase A2 isolated from the venom of the snake Bothrops asper","volume":"84","author":"Gutierrez","year":"1986","journal-title":"Comp. Biochem. Physiol., C"},{"key":"10.1016\/j.biochi.2022.11.006_bib29","first-page":"647","article-title":"Phospholipases A2 from viperidae snake venoms: how do they induce skeletal muscle damage?","volume":"58","author":"Lomonte","year":"2011","journal-title":"Acta Chim. Slov."},{"key":"10.1016\/j.biochi.2022.11.006_bib30","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1080\/13543776.2017.1246540","article-title":"Inhibitors of phospholipase A2 and their therapeutic potential: an update on patents (2012-2016)","volume":"27","author":"Kokotou","year":"2017","journal-title":"Expert Opin. Ther. Pat."},{"key":"10.1016\/j.biochi.2022.11.006_bib31","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/s10557-011-6344-2","article-title":"Anti-inflammatory effects of varespladib methyl in diabetic patients with acute coronary syndrome","volume":"25","author":"Rosenson","year":"2011","journal-title":"Cardiovasc. Drugs Ther."},{"key":"10.1016\/j.biochi.2022.11.006_bib32","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1097\/01.CCM.0000053648.42884.89","article-title":"Efficacy and safety of LY315920Na\/S-5920, a selective inhibitor of 14-kDa group IIA secretory phospholipase A2, in patients with suspected sepsis and organ failure","volume":"31","author":"Abraham","year":"2003","journal-title":"Crit. Care Med."},{"key":"10.1016\/j.biochi.2022.11.006_bib33","first-page":"417","article-title":"A\u00a0randomized, double-blinded, placebo-controlled clinical trial of LY333013, a selective inhibitor of group II secretory phospholipase A2, in the treatment of rheumatoid arthritis","volume":"32","author":"Bradley","year":"2005","journal-title":"J.\u00a0Rheumatol."},{"key":"10.1016\/j.biochi.2022.11.006_bib34","volume":"vol. 8","author":"Lewin","year":"2016"},{"key":"10.1016\/j.biochi.2022.11.006_bib35","article-title":"Delayed oral LY333013 rescues mice from highly neurotoxic","volume":"10","author":"Lewin","year":"2018","journal-title":"Lethal Doses of Papuan Taipan (Oxyuranus scutellatus) Venom, Toxins (Basel)"},{"key":"10.1016\/j.biochi.2022.11.006_bib36","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines8060165","article-title":"Varespladib inhibits the phospholipase A2 and coagulopathic activities of venom components from hemotoxic snakes","volume":"8","author":"Xie","year":"2020","journal-title":"Biomedicines"},{"key":"10.1016\/j.biochi.2022.11.006_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2020.108818","article-title":"Varespladib (LY315920) neutralises phospholipase A2 mediated prothrombinase-inhibition induced by Bitis snake venoms","volume":"236","author":"Youngman","year":"2020","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.biochi.2022.11.006_bib38","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.toxicon.2021.09.009","article-title":"Varespladib (LY315920) prevents neuromuscular blockage and myotoxicity induced by crotoxin on mouse neuromuscular preparations","volume":"202","author":"Maciel","year":"2021","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2018.11.292","article-title":"Neutralizing properties of LY315920 toward snake venom group I and II myotoxic phospholipases A2","volume":"157","author":"Bryan-Quiros","year":"2019","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib40","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-53755-5","article-title":"Structural basis for phospholipase A2-like toxin inhibition by the synthetic compound Varespladib (LY315920)","volume":"9","author":"Salvador","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biochi.2022.11.006_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbagen.2021.129913","article-title":"The synthetic varespladib molecule is a multi-functional inhibitor for PLA2 and PLA2-like ophidic toxins","volume":"1865","author":"Salvador","year":"2021","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.biochi.2022.11.006_bib42","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1006\/abbi.2000.2244","article-title":"Dissociation of enzymatic and pharmacological properties of piratoxins-I and -III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom","volume":"387","author":"Soares","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.biochi.2022.11.006_bib43","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ab.2015.12.013","article-title":"On the acquisition and analysis of microscale thermophoresis data","volume":"496","author":"Scheuermann","year":"2016","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biochi.2022.11.006_bib44","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s00249-021-01529-1","article-title":"Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments","volume":"50","author":"Lopez-Mendez","year":"2021","journal-title":"Eur. Biophys. J."},{"key":"10.1016\/j.biochi.2022.11.006_bib45","series-title":"Introduction to Macromolecular Crystallography","author":"McPherson","year":"2009"},{"key":"10.1016\/j.biochi.2022.11.006_bib46","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1107\/S0907444911007773","article-title":"Data processing and analysis with the autoPROC toolbox","volume":"67","author":"Vonrhein","year":"2011","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"10.1016\/j.biochi.2022.11.006_bib47","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.biochi.2022.11.006_bib48","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"10.1016\/j.biochi.2022.11.006_bib49","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"10.1016\/j.biochi.2022.11.006_bib50","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: more and better reference data for improved all-atom structure validation","volume":"27","author":"Williams","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.biochi.2022.11.006_bib51","series-title":"The PyMOL Molecular Graphics System","author":"Schrodinger","year":"2010"},{"key":"10.1016\/j.biochi.2022.11.006_bib52","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-1-4939-2269-7_19","article-title":"Small-molecule library screening by docking with PyRx","volume":"1263","author":"Dallakyan","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.biochi.2022.11.006_bib53","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"10.1016\/j.biochi.2022.11.006_bib54","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1002\/jcc.23354","article-title":"CHARMM36 all-atom additive protein force field: validation based on comparison to NMR data","volume":"34","author":"Huang","year":"2013","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.biochi.2022.11.006_bib55","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1002\/jcc.20945","article-title":"CHARMM-GUI: a web-based graphical user interface for CHARMM","volume":"29","author":"Jo","year":"2008","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.biochi.2022.11.006_bib56","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J.\u00a0Chem. Theor. Comput."},{"key":"10.1016\/j.biochi.2022.11.006_bib57","article-title":"Canonical sampling through velocity rescaling","volume":"126","author":"Bussi","year":"2007","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.biochi.2022.11.006_bib58","article-title":"Molecular dynamics with coupling to an external bath","volume":"81","author":"Berendsen","year":"1984","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.biochi.2022.11.006_bib59","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1103\/PhysRevA.31.1695","article-title":"Canonical dynamics: equilibrium phase-space distributions","volume":"31","author":"Hoover","year":"1985","journal-title":"Phys Rev A Gen Phys"},{"key":"10.1016\/j.biochi.2022.11.006_bib60","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1080\/00268978400101201","article-title":"A\u00a0molecular dynamics method for simulations in the canonical ensemble","volume":"52","author":"Nos\u00e9","year":"1983","journal-title":"Mol. Phys."},{"key":"10.1016\/j.biochi.2022.11.006_bib61","doi-asserted-by":"crossref","first-page":"7182","DOI":"10.1063\/1.328693","article-title":"Polymorphic transitions in single crystals: a new molecular dynamics method","volume":"52","author":"Parrinello","year":"1981","journal-title":"J.\u00a0Appl. Phys."},{"key":"10.1016\/j.biochi.2022.11.006_bib62","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H","article-title":"LINCS: a linear constraint solver for molecular simulations","volume":"18","author":"Hess","year":"1997","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.biochi.2022.11.006_bib63","volume":"vol. 9","author":"Gutierrez","year":"2017"},{"key":"10.1016\/j.biochi.2022.11.006_bib64","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/s41598-018-36839-6","article-title":"Search for efficient inhibitors of myotoxic activity induced by ophidian phospholipase A2-like proteins using functional, structural and bioinformatics approaches","volume":"9","author":"Salvador","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biochi.2022.11.006_bib65","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1016\/S0006-2952(03)00579-3","article-title":"Inhibitory effect of fucoidan on the activities of crotaline snake venom myotoxic phospholipases A2","volume":"66","author":"Angulo","year":"2003","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.biochi.2022.11.006_bib66","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.jep.2007.04.002","article-title":"Neutralization of snake venom phospholipase A2 toxins by aqueous extract of Casearia sylvestris (Flacourtiaceae) in mouse neuromuscular preparation","volume":"112","author":"Cavalcante","year":"2007","journal-title":"J.\u00a0Ethnopharmacol."},{"key":"10.1016\/j.biochi.2022.11.006_bib67","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.toxicon.2020.08.023","article-title":"Varespladib (LY315920) inhibits neuromuscular blockade induced by Oxyuranus scutellatus venom in a nerve-muscle preparation","volume":"187","author":"Fontana Oliveira","year":"2020","journal-title":"Toxicon"},{"key":"10.1016\/j.biochi.2022.11.006_bib68","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1432-1033.1976.tb10833.x","article-title":"Taipoxin, an extremely potent presynaptic neurotoxin from the venom of the australian snake taipan (Oxyuranus s. scutellatus). Isolation, characterization, quaternary structure and pharmacological properties","volume":"68","author":"Fohlman","year":"1976","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.biochi.2022.11.006_bib69","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.biochi.2017.07.009","article-title":"Structural studies with BnSP-7 reveal an atypical oligomeric conformation compared to phospholipases A2-like toxins","volume":"142","author":"de Lima","year":"2017","journal-title":"Biochimie"},{"key":"10.1016\/j.biochi.2022.11.006_bib70","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1016\/j.bbagen.2016.08.003","article-title":"Functional and structural studies of a Phospholipase A2-like protein complexed to zinc ions: insights on its myotoxicity and inhibition mechanism","volume":"1861","author":"Borges","year":"2017","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biochi.2022.11.006_bib71","doi-asserted-by":"crossref","first-page":"2728","DOI":"10.1016\/j.bbagen.2018.08.002","article-title":"Structural basis of phospholipase A2-like myotoxin inhibition by chicoric acid, a novel potent inhibitor of ophidian toxins","volume":"1862","author":"Cardoso","year":"2018","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.biochi.2022.11.006_bib72","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.biochi.2020.12.008","article-title":"In-solution structural studies involving a phospholipase A2-like myotoxin and a natural inhibitor: plasticity of oligomeric assembly affects mechanisms of inhibition","volume":"181","author":"Cardoso","year":"2020","journal-title":"Biochimie"},{"key":"10.1016\/j.biochi.2022.11.006_bib73","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","article-title":"Inference of macromolecular assemblies from crystalline state","volume":"372","author":"Krissinel","year":"2007","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.biochi.2022.11.006_bib74","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.biochi.2020.01.010","article-title":"Neutralization of a bothropic PLA2-like protein by caftaric acid, a novel potent inhibitor of ophidian myotoxicity","volume":"170","author":"Cardoso","year":"2020","journal-title":"Biochimie"},{"key":"10.1016\/j.biochi.2022.11.006_bib75","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0028521","article-title":"Structural and functional studies of a bothropic myotoxin complexed to rosmarinic acid: new insights into Lys49-PLA2 inhibition","volume":"6","author":"Dos Santos","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.biochi.2022.11.006_bib76","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0133370","article-title":"Structural basis for the inhibition of a phospholipase A2-like toxin by caffeic and aristolochic acids","volume":"10","author":"Fernandes","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.biochi.2022.11.006_bib77","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.jmb.2005.04.072","article-title":"Inhibition of myotoxic activity of Bothrops asper myotoxin II by the anti-trypanosomal drug suramin","volume":"350","author":"Murakami","year":"2005","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.biochi.2022.11.006_bib78","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-28584-7","article-title":"Structural and functional characterization of suramin-bound MjTX-I from Bothrops moojeni suggests a particular myotoxic mechanism","volume":"8","author":"Salvador","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biochi.2022.11.006_bib79","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.bbapap.2017.12.008","article-title":"Structural evidence for a fatty acid-independent myotoxic mechanism for a phospholipase A2-like toxin","volume":"1866","author":"Salvador","year":"2018","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biochi.2022.11.006_bib80","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.ijbiomac.2021.06.163","article-title":"Gallic acid anti-myotoxic activity and mechanism of action, a snake venom phospholipase A2 toxin inhibitor, isolated from the medicinal plant Anacardium humile","volume":"185","author":"Costa","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.biochi.2022.11.006_bib81","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.xphs.2019.10.056","article-title":"Technical capabilities and limitations of optical Spectroscopy and calorimetry using water-miscible solvents: the case of dimethyl Sulfoxide, acetonitrile, and 1,4-dioxane","volume":"109","author":"Hirano","year":"2020","journal-title":"J\u00a0Pharm Sci"}],"container-title":["Biochimie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908422002930?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908422002930?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:23:34Z","timestamp":1759541014000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300908422002930"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":81,"alternative-id":["S0300908422002930"],"URL":"https:\/\/doi.org\/10.1016\/j.biochi.2022.11.006","relation":{},"ISSN":["0300-9084"],"issn-type":[{"value":"0300-9084","type":"print"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural basis of the myotoxic inhibition of the Bothrops pirajai PrTX-I by the synthetic varespladib","name":"articletitle","label":"Article Title"},{"value":"Biochimie","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biochi.2022.11.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. and Soci\u00e9t\u00e9 Fran\u00e7aise de Biochimie et Biologie Mol\u00e9culaire (SFBBM). All rights reserved.","name":"copyright","label":"Copyright"}]}}