{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T16:04:08Z","timestamp":1780589048527,"version":"3.54.1"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2024MC10"],"award-info":[{"award-number":["ZR2024MC10"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2021QC089"],"award-info":[{"award-number":["ZR2021QC089"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018589","name":"Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018589","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31873014"],"award-info":[{"award-number":["31873014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32301261"],"award-info":[{"award-number":["32301261"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimie"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.biochi.2026.01.013","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:16:54Z","timestamp":1769732214000},"page":"29-39","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["The regulation characterization of novel transcription regulator JTY_2262 in Mycobacterium bovis BCG"],"prefix":"10.1016","volume":"244","author":[{"given":"Jinmiao","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8733-9763","authenticated-orcid":false,"given":"Mingrui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayin","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siguo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoli","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biochi.2026.01.013_bib1","year":"2024","journal-title":"Global Tuberculosis Report 2024."},{"key":"10.1016\/j.biochi.2026.01.013_bib2","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00141-20","article-title":"Mechanisms of drug-induced tolerance in Mycobacterium tuberculosis","volume":"34","author":"Goossens","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.biochi.2026.01.013_bib3","doi-asserted-by":"crossref","first-page":"794","DOI":"10.4161\/viru.27794","article-title":"Transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide","volume":"5","author":"Green","year":"2014","journal-title":"Virulence."},{"key":"10.1016\/j.biochi.2026.01.013_bib4","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/j.tim.2019.06.005","article-title":"Acid fasting: Modulation of Mycobacterium tuberculosis metabolism at acidic pH","volume":"27","author":"Baker","year":"2019","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib5","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1128\/MMBR.00018-13","article-title":"The TetR family of regulators","volume":"77","author":"Cuthbertson","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.biochi.2026.01.013_bib6","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.990312","article-title":"Transcriptional regulation and drug resistance in Mycobacterium tuberculosis","volume":"12","author":"Miotto","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib7","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1002\/prot.26621","article-title":"TetR and OmpR family regulators in natural product biosynthesis and resistance","volume":"93","author":"Patil","year":"2025","journal-title":"Proteins"},{"key":"10.1016\/j.biochi.2026.01.013_bib8","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1128\/MMBR.69.2.326-356.2005","article-title":"The TetR family of transcriptional repressors","volume":"69","author":"Ramos","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.biochi.2026.01.013_bib9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/21541264.2023.2293523","article-title":"Targeting bacterial transcription factors for infection control: opportunities and challenges","volume":"16","author":"Al-Tohamy","year":"2023","journal-title":"Transcription"},{"key":"10.1016\/j.biochi.2026.01.013_bib10","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2111059119","article-title":"MadR mediates acyl CoA-dependent regulation of mycolic acid desaturation in mycobacteria","volume":"119","author":"Cooper","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.biochi.2026.01.013_bib11","doi-asserted-by":"crossref","first-page":"811","DOI":"10.4014\/jmb.1911.11043","article-title":"Transcriptional and mycolic acid profiling in Mycobacterium bovis BCG in vitro show an effect for c-di-GMP and overlap between dormancy and biofilms","volume":"30","author":"Cruz-Villegas","year":"2020","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.biochi.2026.01.013_bib12","doi-asserted-by":"crossref","first-page":"7239","DOI":"10.1038\/s41467-023-42823-0","article-title":"Structures of the DarR transcription regulator reveal unique modes of second messenger and DNA binding","volume":"14","author":"Schumacher","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biochi.2026.01.013_bib13","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1016\/j.cellsig.2013.04.003","article-title":"The underling mechanism of bacterial TetR\/AcrR family transcriptional repressors","volume":"25","author":"Deng","year":"2013","journal-title":"Cell. Signal."},{"key":"10.1016\/j.biochi.2026.01.013_bib14","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1089\/dna.2017.4066","article-title":"Insights into macrophage autophagy in latent tuberculosis infection: role of heat shock protein 16.3","volume":"37","author":"Zhang","year":"2018","journal-title":"DNA Cell Biol."},{"key":"10.1016\/j.biochi.2026.01.013_bib15","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1007\/s00284-022-02949-8","article-title":"Evaluating the performance of PPE44, HSPX, ESAT-6 and CFP-10 factors in tuberculosis subunit vaccines","volume":"79","author":"Valizadeh","year":"2022","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2023.111060","article-title":"Construction and evaluation of a novel multi-antigenic Mycobacterium tuberculosis subunit vaccine candidate BfrB-GrpE\/DPC","volume":"124","author":"Niu","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.biochi.2026.01.013_bib17","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/351456a0","article-title":"New use of BCG for recombinant vaccines","volume":"351","author":"Stover","year":"1991","journal-title":"Nature"},{"key":"10.1016\/j.biochi.2026.01.013_bib18","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2023.1292864","article-title":"DosR's multifaceted role on Mycobacterium bovis BCG revealed through multi-omics","volume":"13","author":"Cui","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib19","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods. (San Diego, Calif)"},{"key":"10.1016\/j.biochi.2026.01.013_bib20","doi-asserted-by":"crossref","first-page":"5237","DOI":"10.1128\/JB.00989-12","article-title":"Characterization of a new GlnR binding box in the promoter of amtB in Streptomyces coelicolor inferred a PhoP\/GlnR competitive binding mechanism for transcriptional regulation of amtB","volume":"194","author":"Wang","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.biochi.2026.01.013_bib21","article-title":"Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from Mycobacterium bovis BCG","volume":"15","author":"Wang","year":"2024","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib22","doi-asserted-by":"crossref","first-page":"4682","DOI":"10.1021\/pr3006233","article-title":"A genome-wide regulator-DNA interaction network in the human pathogen Mycobacterium tuberculosis H37Rv","volume":"11","author":"Zeng","year":"2012","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.biochi.2026.01.013_bib23","doi-asserted-by":"crossref","first-page":"812","DOI":"10.3389\/fmicb.2020.00812","article-title":"Vitamin B and vitamin C affect DNA methylation and amino acid metabolism in Mycobacterium bovis BCG","volume":"11","author":"Song","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib24","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1016\/j.bbrc.2016.08.049","article-title":"M. tuberculosis ferritin (rv3841): potential involvement in amikacin (AK) & kanamycin (KM) resistance","volume":"478","author":"Sharma","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.biochi.2026.01.013_bib25","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/3525302","article-title":"Mycobacterium tuberculosis protein Rv3841 activates dendritic cells and contributes to a T helper 1 immune response","volume":"2018","author":"Choi","year":"2018","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.biochi.2026.01.013_bib26","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1099\/mic.0.28993-0","article-title":"Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress","volume":"152","author":"Sirakova","year":"2006","journal-title":"Microbiol. (Reading, England)"},{"key":"10.1016\/j.biochi.2026.01.013_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijmm.2020.151402","article-title":"ClpB is an essential stress regulator of Mycobacterium tuberculosis and endows survival advantage to dormant bacilli","volume":"310","author":"Tripathi","year":"2020","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib28","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1002\/2211-5463.12509","article-title":"The amino-terminal domain of Mycobacterium tuberculosis ClpB protein plays a crucial role in its substrate disaggregation activity","volume":"8","author":"Tripathi","year":"2018","journal-title":"FEBS. open. bio."},{"key":"10.1016\/j.biochi.2026.01.013_bib29","article-title":"MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs, Protein","volume":"191","author":"Ung","year":"2022","journal-title":"Expr. Purif."},{"key":"10.1016\/j.biochi.2026.01.013_bib30","article-title":"Corrigendum to \u201cMmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs\u201d","author":"Ung","year":"2022","journal-title":"Protein Expr. Purif."},{"issue":"Pt 5","key":"10.1016\/j.biochi.2026.01.013_bib31","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1107\/S2053230X19004618","article-title":"Novel T9 loop conformation of filamenting temperature-sensitive mutant Z from Mycobacterium tuberculosis, acta","volume":"75","author":"Lazo","year":"2019","journal-title":"Crystallogr. F. Struct. Biol. Commun."},{"key":"10.1016\/j.biochi.2026.01.013_bib32","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1128\/IAI.00520-12","article-title":"MprA and DosR coregulate a Mycobacterium tuberculosis virulence operon encoding Rv1813c and Rv1812c","volume":"80","author":"Bretl","year":"2012","journal-title":"Infect. Immun."},{"key":"10.1016\/j.biochi.2026.01.013_bib33","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3390\/pathogens9030166","article-title":"MmpS5-MmpL5 transporters provide Mycobacterium smegmatis resistance to imidazo[1,2-b][1,2,4,5]tetrazines","volume":"9","author":"Maslov","year":"2020","journal-title":"Pathogens."},{"key":"10.1016\/j.biochi.2026.01.013_bib34","doi-asserted-by":"crossref","first-page":"22315","DOI":"10.1074\/jbc.M116.744532","article-title":"Post-translational acetylation of MbtA modulates mycobacterial siderophore biosynthesis","volume":"291","author":"Vergnolle","year":"2016","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.biochi.2026.01.013_bib35","article-title":"Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages","volume":"17","author":"Jiang","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"10.1016\/j.biochi.2026.01.013_bib36","doi-asserted-by":"crossref","first-page":"5237","DOI":"10.1016\/j.vaccine.2010.05.063","article-title":"Enhanced protection against tuberculosis by vaccination with recombinant BCG over-expressing HspX protein","volume":"28","author":"Shi","year":"2010","journal-title":"Vaccine."},{"key":"10.1016\/j.biochi.2026.01.013_bib37","doi-asserted-by":"crossref","first-page":"10587","DOI":"10.1007\/s00253-015-6962-x","article-title":"A live attenuated BCG vaccine overexpressing multistage antigens Ag85B and HspX provides superior protection against Mycobacterium tuberculosis infection","volume":"99","author":"Yuan","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biochi.2026.01.013_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0075684","article-title":"ESAT-6 and HspX improve the effectiveness of BCG to induce human dendritic cells-dependent Th1 and NK cells activation","volume":"8","author":"Marongiu","year":"2013","journal-title":"PLoS One."},{"key":"10.1016\/j.biochi.2026.01.013_bib39","doi-asserted-by":"crossref","first-page":"5655","DOI":"10.1016\/j.vaccine.2005.03.056","article-title":"Elicitation of efficient, protective immune responses by using DNA vaccines against tuberculosis","volume":"23","author":"Khera","year":"2005","journal-title":"Vaccine."},{"key":"10.1016\/j.biochi.2026.01.013_bib40","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1128\/IAI.74.2.861-868.2006","article-title":"Deletion of the Mycobacterium tuberculosis \u03b1-Crystallin-Like hspX gene causes increased bacterial growth in Vivo","volume":"74","author":"Hu","year":"2006","journal-title":"Infect. Immun."},{"key":"10.1016\/j.biochi.2026.01.013_bib41","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1186\/s12864-015-1696-9","article-title":"Global analyses of TetR family transcriptional regulators in mycobacteria indicates conservation across species and diversity in regulated functions","volume":"16","author":"Balhana","year":"2015","journal-title":"BMC Genom."},{"key":"10.1016\/j.biochi.2026.01.013_bib42","doi-asserted-by":"crossref","first-page":"958","DOI":"10.3389\/fmicb.2016.00958","article-title":"Relationships between the regulatory systems of quorum sensing and multidrug resistance","volume":"7","author":"Xu","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biochi.2026.01.013_bib43","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/j.jmb.2010.05.062","article-title":"A comprehensive analysis of structural and sequence conservation in the TetR family transcriptional regulators","volume":"400","author":"Yu","year":"2010","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.biochi.2026.01.013_bib44","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1089\/dna.2015.3040","article-title":"New targets and cofactors for the transcription factor LrpA from Mycobacterium tuberculosis","volume":"35","author":"Song","year":"2016","journal-title":"DNA Cell Biol."},{"key":"10.1016\/j.biochi.2026.01.013_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.chembiol.2021.11.004","article-title":"An allosteric inhibitor of bacterial Hsp70 chaperone potentiates antibiotics and mitigates resistance","volume":"29","author":"Hosfelt","year":"2022","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.biochi.2026.01.013_bib46","doi-asserted-by":"crossref","first-page":"732","DOI":"10.3390\/biom13050732","article-title":"Structural and biochemical characterization of Mycobacterium tuberculosis zinc SufU-SufS complex","volume":"13","author":"Elchennawi","year":"2023","journal-title":"Biomolecules."}],"container-title":["Biochimie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908426000222?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908426000222?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:49:33Z","timestamp":1780588173000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300908426000222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":46,"alternative-id":["S0300908426000222"],"URL":"https:\/\/doi.org\/10.1016\/j.biochi.2026.01.013","relation":{},"ISSN":["0300-9084"],"issn-type":[{"value":"0300-9084","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The regulation characterization of novel transcription regulator JTY_2262 in Mycobacterium bovis BCG","name":"articletitle","label":"Article Title"},{"value":"Biochimie","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biochi.2026.01.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. and Soci\u00e9t\u00e9 Fran\u00e7aise de Biochimie et Biologie Mol\u00e9culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}