{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:44:52Z","timestamp":1792788292604,"version":"4.5.0"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100011789","name":"Jilin Provincial Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimie"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.biochi.2026.10.006","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:14:53Z","timestamp":1792106093000},"update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rapid and specific RAA-CRISPR\/Cas13a assay for detecting Streptococcus mutans"],"prefix":"10.1016","author":[{"given":"Xingdong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjun","family":"Bu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixing","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanna","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingshuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biochi.2026.10.006_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.microb.2025.100320","article-title":"Designing multi-epitope-based vaccine targeting immunogenic proteins of Streptococcus mutans using immunoinformatics to prevent caries","volume":"7","author":"Kottarathil","year":"2025","journal-title":"The Microbe"},{"key":"10.1016\/j.biochi.2026.10.006_bib2","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.4014\/jmb.2304.04026","article-title":"Rapid detection of Streptococcus mutans using an integrated microfluidic system with loop-mediated isothermal amplification","volume":"33","author":"Wang","year":"2023","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.biochi.2026.10.006_bib3","doi-asserted-by":"crossref","first-page":"3535","DOI":"10.1007\/s00784-020-03676-9","article-title":"Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals","volume":"25","author":"Lima","year":"2021","journal-title":"Clin. Oral Invest."},{"key":"10.1016\/j.biochi.2026.10.006_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2020.113812","article-title":"Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification","volume":"605","author":"Kitagawa","year":"2020","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biochi.2026.10.006_bib5","doi-asserted-by":"crossref","DOI":"10.31744\/einstein_journal\/2019AO4515","article-title":"Streptococcus mutans detection in saliva and colostrum samples","volume":"17","author":"da Silva","year":"2019","journal-title":"Einstein (Sao Paulo)"},{"key":"10.1016\/j.biochi.2026.10.006_bib6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1099\/00222615-41-4-231","article-title":"Detection of Streptococcus mutans by PCR amplification of spaP gene","volume":"41","author":"Ono","year":"1994","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.biochi.2026.10.006_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2024.109480","article-title":"CRISPR: the frontier technology of next-generation RNA detection","volume":"211","author":"Zhou","year":"2024","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.biochi.2026.10.006_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2023.341757","article-title":"One-tube RPA-CRISPR Cas12a\/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus","volume":"1278","author":"Liu","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.biochi.2026.10.006_bib9","doi-asserted-by":"crossref","first-page":"649","DOI":"10.3390\/cimb45010043","article-title":"Molecular mechanisms underlying CRISPR\/Cas-Based assays for nucleic acid detection","volume":"45","author":"Antropov","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.biochi.2026.10.006_bib10","article-title":"CRISPR\/Cas-based nucleic acid detection strategies: trends and challenges","volume":"10","author":"Zhou","year":"2024","journal-title":"Heliyon"},{"key":"10.1016\/j.biochi.2026.10.006_bib11","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.biochi.2026.10.006_bib12","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1038\/s41467-024-47281-w","article-title":"The CRISPR-Cas13a gemini system for noncontiguous target RNA activation","volume":"15","author":"Zhao","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biochi.2026.10.006_bib13","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1111\/2041-210X.14369","article-title":"Rapid, easy, sensitive, low-cost and on-site detection of environmental DNA and RNA using CRISPR -Cas13","volume":"15","author":"Yang","year":"2024","journal-title":"Methods Ecol. Evol."},{"key":"10.1016\/j.biochi.2026.10.006_bib14","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nature19802","article-title":"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection","volume":"538","author":"East-Seletsky","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.biochi.2026.10.006_bib15","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/s41589-024-01593-6","article-title":"Structures, mechanisms and applications of RNA-centric CRISPR-Cas13","volume":"20","author":"Yang","year":"2024","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.biochi.2026.10.006_bib16","doi-asserted-by":"crossref","first-page":"10522","DOI":"10.1021\/acs.analchem.2c05032","article-title":"Strategies to improve multi-enzyme compatibility and coordination in one-pot SHERLOCK","volume":"95","author":"Li","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.biochi.2026.10.006_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.micres.2022.127000","article-title":"CRISPR Cas system: a strategic approach in detection of nucleic acids","volume":"259","author":"Fapohunda","year":"2022","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.biochi.2026.10.006_bib18","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1273988","article-title":"Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection","volume":"11","author":"Mao","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.biochi.2026.10.006_bib19","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1007\/s11427-021-2028-x","article-title":"A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13","volume":"65","author":"Li","year":"2022","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.biochi.2026.10.006_bib20","doi-asserted-by":"crossref","first-page":"3873","DOI":"10.1021\/acs.analchem.2c05632","article-title":"Elucidating the role of CRISPR\/Cas in single-step isothermal nucleic acid amplification testing assays","volume":"95","author":"Shao","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.biochi.2026.10.006_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2022.123388","article-title":"Integrating CRISPR\/Cas within isothermal amplification for point-of-care Assay of nucleic acid","volume":"243","author":"Zhang","year":"2022","journal-title":"Talanta"},{"key":"10.1016\/j.biochi.2026.10.006_bib22","article-title":"An anchored CRISPR\/Cas12a system-boosted paper-based microfluidic platform for on-site detection of Streptococcus mutans","volume":"520","author":"Zhang","year":"2025","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biochi.2026.10.006_bib23","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.snb.2017.06.106","article-title":"A sandwich protocol for chemiluminescent detection of Streptococcus mutans utilizing dual-site recognition by rat IgG2a and vancomycin","volume":"252","author":"Yang","year":"2017","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.biochi.2026.10.006_bib24","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.talanta.2017.11.004","article-title":"Specific chemiluminescent protocol for dual-site recognition of Streptococcus mutans utilizing strong affinity between teicoplanin and Gram-positive bacteria","volume":"179","author":"Su","year":"2018","journal-title":"Talanta"},{"key":"10.1016\/j.biochi.2026.10.006_bib25","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.snb.2016.10.042","article-title":"Bioluminescent detection of the total amount of viable Gram-positive bacteria isolated by vancomycin-functionalized magnetic particles","volume":"241","author":"Su","year":"2017","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.biochi.2026.10.006_bib26","doi-asserted-by":"crossref","first-page":"9780","DOI":"10.1021\/acs.analchem.3c05112","article-title":"Portable saliva sensor based on dual recognition elements for detection of caries pathogenic bacteria","volume":"96","author":"Li","year":"2024","journal-title":"Anal. Chem."}],"container-title":["Biochimie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908426002439?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300908426002439?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:14:40Z","timestamp":1792786480000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300908426002439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":26,"alternative-id":["S0300908426002439"],"URL":"https:\/\/doi.org\/10.1016\/j.biochi.2026.10.006","relation":{},"ISSN":["0300-9084"],"issn-type":[{"value":"0300-9084","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Rapid and specific RAA-CRISPR\/Cas13a assay for detecting Streptococcus mutans","name":"articletitle","label":"Article Title"},{"value":"Biochimie","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biochi.2026.10.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. and Soci\u00e9t\u00e9 Fran\u00e7aise de Biochimie et Biologie Mol\u00e9culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}