{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:16:43Z","timestamp":1791461803632,"version":"4.3.4"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioelectrochemistry"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.bioelechem.2024.108751","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T06:30:32Z","timestamp":1716964232000},"page":"108751","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Dihydroethidium-derived fluorescence in electrically stressed cells indicates intracellular microenvironment modifications independent of ROS"],"prefix":"10.1016","volume":"160","author":[{"given":"Esin B.","family":"S\u00f6zer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iurii","family":"Semenov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Thomas","family":"Vernier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bioelechem.2024.108751_b0005","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1471-2121-7-37","article-title":"Nanoelectropulse-driven membrane perturbation and small molecule permeabilization","volume":"7","author":"Vernier","year":"2006","journal-title":"BMC Cell Biol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0010","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/bem.20354","article-title":"Long-lasting plasma membrane permeabilization in mammalian cells by nanosecond Pulsed Electric Field (nsPEF)","volume":"28","author":"Pakhomov","year":"2007","journal-title":"Bioelectromagnetics"},{"key":"10.1016\/j.bioelechem.2024.108751_b0015","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1007\/s00018-016-2434-4","article-title":"Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types","volume":"74","author":"Gianulis","year":"2017","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bioelechem.2024.108751_b0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00232-016-9918-x","article-title":"Nanometer-scale permeabilization and osmotic swelling induced by 5-ns pulsed electric fields","volume":"250","author":"S\u00f6zer","year":"2017","journal-title":"J. Membr. Biol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0025","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.abb.2007.05.003","article-title":"Membrane permeabilization and cell damage by ultrashort electric field shocks","volume":"465","author":"Pakhomov","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bioelechem.2024.108751_b0030","doi-asserted-by":"crossref","first-page":"3608","DOI":"10.1529\/biophysj.105.072777","article-title":"Plasma membrane voltage changes during nanosecond pulsed electric field exposure","volume":"90","author":"Frey","year":"2006","journal-title":"Biophys. J."},{"key":"10.1016\/j.bioelechem.2024.108751_b0035","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s11517-010-0599-9","article-title":"Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells","volume":"48","author":"Napotnik","year":"2010","journal-title":"Med. Biol. Eng. Comput."},{"key":"10.1016\/j.bioelechem.2024.108751_b0040","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.bbrc.2003.08.140","article-title":"Calcium bursts induced by nanosecond electric pulses","volume":"310","author":"Vernier","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bioelechem.2024.108751_b0045","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M311135200","article-title":"Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields","volume":"279","author":"White","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bioelechem.2024.108751_b0050","first-page":"981","article-title":"Primary pathways of intracellular Ca2+ mobilization by nanosecond pulsed electric field","volume":"2013","author":"Semenov","year":"1828","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"10.1016\/j.bioelechem.2024.108751_b0055","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/S0006-3495(90)82451-6","article-title":"Electropermeabilization of mammalian cells. Quantitative analysis of the phenomenon","volume":"58","author":"Rols","year":"1990","journal-title":"Biophys. J."},{"key":"10.1016\/j.bioelechem.2024.108751_b0060","article-title":"Correction: dose-dependent ATP depletion and cancer cell death following calcium electroporation, relative effect of calcium concentration and electric field strength","volume":"10","author":"Hansen","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.bioelechem.2024.108751_b0065","doi-asserted-by":"crossref","DOI":"10.1109\/TPS.2002.1003872","article-title":"Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition","volume":"30","author":"Beebe","year":"2002","journal-title":"IEEE Trans. Plasma Sci."},{"key":"10.1016\/j.bioelechem.2024.108751_b0070","doi-asserted-by":"crossref","first-page":"6288","DOI":"10.1021\/ja0588306","article-title":"Nanopore formation and phosphatidylserine externalization in a phospholipid bilayer at high transmembrane potential","volume":"128","author":"Vernier","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bioelechem.2024.108751_b0075","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1007\/s10571-010-9573-1","article-title":"Nanosecond electric pulses: a novel stimulus for triggering Ca2+ influx into chromaffin cells via voltage-gated Ca2+ channels","volume":"30","author":"Craviso","year":"2010","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0080","first-page":"2175","article-title":"Expression of voltage-gated calcium channels augments cell susceptibility to membrane disruption by nanosecond pulsed electric field","volume":"2018","author":"Hristov","year":"1860","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"10.1016\/j.bioelechem.2024.108751_b0085","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.bioelechem.2003.10.006","article-title":"Supra-physiological membrane potential induced conformational changes in K+ channel conducting system of skeletal muscle fibers","volume":"62","author":"Chen","year":"2004","journal-title":"Bioelectrochemistry."},{"key":"10.1016\/j.bioelechem.2024.108751_b0090","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.burns.2005.08.008","article-title":"Supramembrane potential-induced electroconformational changes in sodium channel proteins: a potential mechanism involved in electric injury","volume":"32","author":"Chen","year":"2006","journal-title":"Burns"},{"key":"10.1016\/j.bioelechem.2024.108751_b0095","doi-asserted-by":"crossref","DOI":"10.1016\/j.bpj.2020.05.030","article-title":"Pulsed electric fields can create pores in the voltage sensors of voltage-gated ion channels","author":"Rems","year":"2020","journal-title":"Biophys. J."},{"key":"10.1016\/j.bioelechem.2024.108751_b0100","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2021.107837","article-title":"The role of ESCRT-III and Annexin V in the repair of cell membrane permeabilization by the nanosecond pulsed electric field","volume":"140","author":"Muratori","year":"2021","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.bioelechem.2024.108751_b0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbamem.2021.183823","article-title":"Ca2+ dependence and kinetics of cell membrane repair after electropermeabilization","volume":"1864","author":"Bhattacharya","year":"2022","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"10.1016\/j.bioelechem.2024.108751_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2021.107871","article-title":"Cell death due to electroporation \u2013 a review","volume":"141","author":"Batista Napotnik","year":"2021","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.bioelechem.2024.108751_b0115","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/s41598-017-00092-0","article-title":"Quantitative limits on small molecule transport via the electropermeome measuring and modeling single nanosecond perturbations","volume":"7","author":"S\u00f6zer","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bioelechem.2024.108751_b0120","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1432-1033.1994.tb18962.x","article-title":"Generation of reactive-oxygen species induced by electropermeabilization of Chinese hamster ovary cells and their consequence on cell viability","volume":"223","author":"Gabriel","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bioelechem.2024.108751_b0125","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1111\/j.1432-1033.1996.0737w.x","article-title":"Electropermeabilization of intact maize cells induces an oxidative stress","volume":"238","author":"Sabri","year":"1996","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bioelechem.2024.108751_b0130","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0005-2736(99)00154-6","article-title":"The generation of reactive-oxygen species associated with long-lasting pulse-induced electropermeabilisation of mammalian cells is based on a non-destructive alteration of the plasma membrane","volume":"1461","author":"Bonnafous","year":"1999","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"10.1016\/j.bioelechem.2024.108751_b0135","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1021\/la8025057","article-title":"Calcium binding and head group dipole angle in phosphatidylserine-phosphatidylcholine bilayers","volume":"25","author":"Vernier","year":"2009","journal-title":"Langmuir"},{"key":"10.1016\/j.bioelechem.2024.108751_b0140","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.abb.2012.08.004","article-title":"Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media","volume":"527","author":"Pakhomova","year":"2012","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bioelechem.2024.108751_b0145","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.bioelechem.2018.07.018","article-title":"The contribution of lipid peroxidation to membrane permeability in electropermeabilization: a molecular dynamics study","volume":"125","author":"Rems","year":"2019","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.bioelechem.2024.108751_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2019.107433","article-title":"Electropermeabilization does not correlate with plasma membrane lipid oxidation","volume":"132","author":"Michel","year":"2020","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.bioelechem.2024.108751_b0155","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s40425-017-0234-5","article-title":"Nano-pulse stimulation is a physical modality that can trigger immunogenic tumor cell death","volume":"5","author":"Nuccitelli","year":"2017","journal-title":"J. Immunother. Cancer"},{"key":"10.1016\/j.bioelechem.2024.108751_b0160","doi-asserted-by":"crossref","DOI":"10.3390\/cancers11122034","article-title":"Nanosecond pulsed electric fields induce endoplasmic reticulum stress accompanied by immunogenic cell death in murine models of lymphoma and colorectal cancer","volume":"11","author":"Rossi","year":"2019","journal-title":"Cancers (basel)"},{"key":"10.1016\/j.bioelechem.2024.108751_b0165","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-immunol-032712-100008","article-title":"Immunogenic cell death in cancer therapy","volume":"31","author":"Kroemer","year":"2013","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0170","doi-asserted-by":"crossref","DOI":"10.1038\/nrc3380","article-title":"Immunogenic cell death and DAMPs in cancer therapy","volume":"12","author":"Krysko","year":"2012","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.bioelechem.2024.108751_b0175","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1189\/jlb.3VMA1215-579RR","article-title":"Oxidized phosphatidylcholine induces the activation of NLRP3 inflammasome in macrophages","volume":"101","author":"Yeon","year":"2017","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0180","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/s41577-021-00618-8","article-title":"Innate immune detection of lipid oxidation as a threat assessment strategy","volume":"22","author":"Zhivaki","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0185","first-page":"85","article-title":"Oxidative damage of the membrane lipids after electroporation","volume":"13","author":"Benov","year":"1994","journal-title":"Gen. Physiol. Biophys."},{"key":"10.1016\/j.bioelechem.2024.108751_b0190","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1023\/A:1015363220266","article-title":"Pitfalls in the use of common luminescent probes for oxidative and nitrosative stress","volume":"12","author":"Wardman","year":"2002","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bioelechem.2024.108751_b0195","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.freeradbiomed.2010.01.028","article-title":"Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth","volume":"48","author":"Zielonka","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bioelechem.2024.108751_b0200","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.freeradbiomed.2011.09.030","article-title":"Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations","volume":"52","author":"Kalyanaraman","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bioelechem.2024.108751_b0205","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.freeradbiomed.2014.10.504","article-title":"Even free radicals should follow some rules: a Guide to free radical research terminology and methodology","volume":"78","author":"Forman","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bioelechem.2024.108751_b0210","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0014-5793(97)00036-7","article-title":"Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study","volume":"403","author":"V\u00e1squez-Vivar","year":"1997","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bioelechem.2024.108751_b0215","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1016\/S0891-5849(98)00121-X","article-title":"Lucigenin as mediator of superoxide production: revisited","volume":"25","author":"Liochev","year":"1998","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bioelechem.2024.108751_b0220","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1109\/TBME.2012.2228650","article-title":"Moveable wire electrode microchamber for nanosecond pulsed electric-field delivery","volume":"60","author":"Wu","year":"2013","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"10.1016\/j.bioelechem.2024.108751_b0225","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1016\/j.bpj.2020.09.028","article-title":"Dye transport through bilayers agrees with lipid electropore molecular dynamics","volume":"119","author":"S\u00f6zer","year":"2020","journal-title":"Biophys. J."},{"key":"10.1016\/j.bioelechem.2024.108751_b0230","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ceca.2013.05.008","article-title":"Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: the impact of pulse duration","volume":"54","author":"Semenov","year":"2013","journal-title":"Cell Calcium"},{"key":"10.1016\/j.bioelechem.2024.108751_b0235","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.bioelechem.2018.01.013","article-title":"Electropermeabilization of cells by closely spaced paired nanosecond-range pulses","volume":"121","author":"Semenov","year":"2018","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.bioelechem.2024.108751_b0240","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/bem.20707","article-title":"Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells","volume":"33","author":"Napotnik","year":"2012","journal-title":"Bioelectromagnetics"},{"key":"10.1016\/j.bioelechem.2024.108751_b0245","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1016\/S0006-3495(96)79472-9","article-title":"Dynamical change of mitochondrial DNA induced in the living cell by perturbing the electrochemical gradient","volume":"71","author":"Coppey-Moisan","year":"1996","journal-title":"Biophys. J."},{"key":"10.1016\/j.bioelechem.2024.108751_b0250","doi-asserted-by":"crossref","first-page":"C1440","DOI":"10.1152\/ajpcell.00607.2004","article-title":"Inhibition of complex I of the electron transport chain causes O2\u2212\u00b7-mediated mitochondrial outgrowth","volume":"288","author":"Koopman","year":"2005","journal-title":"Am. J. Physiol. Physiol."},{"key":"10.1016\/j.bioelechem.2024.108751_b0255","doi-asserted-by":"crossref","first-page":"13599","DOI":"10.1038\/ncomms13599","article-title":"A redox mechanism underlying nucleolar stress sensing by nucleophosmin","volume":"7","author":"Yang","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bioelechem.2024.108751_b0260","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.molcel.2010.09.024","article-title":"The nucleolus under stress","volume":"40","author":"Boulon","year":"2010","journal-title":"Mol. Cell"},{"key":"10.1016\/j.bioelechem.2024.108751_b0265","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1016\/S0006-3495(00)76806-8","article-title":"Restrained torsional dynamics of nuclear DNA in living proliferative mammalian cells","volume":"78","author":"Tramier","year":"2000","journal-title":"Biophys. J."}],"container-title":["Bioelectrochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567539424001130?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567539424001130?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T17:45:03Z","timestamp":1739382303000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567539424001130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":53,"alternative-id":["S1567539424001130"],"URL":"https:\/\/doi.org\/10.1016\/j.bioelechem.2024.108751","relation":{},"ISSN":["1567-5394"],"issn-type":[{"value":"1567-5394","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dihydroethidium-derived fluorescence in electrically stressed cells indicates intracellular microenvironment modifications independent of ROS","name":"articletitle","label":"Article Title"},{"value":"Bioelectrochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioelechem.2024.108751","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"108751"}}