{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T07:18:55Z","timestamp":1769584735087,"version":"3.49.0"},"reference-count":94,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022012","name":"CSIR - Institute of Microbial Technology","doi-asserted-by":"publisher","award":["OLP0554"],"award-info":[{"award-number":["OLP0554"]}],"id":[{"id":"10.13039\/501100022012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["BMI\/11(48)\/2022"],"award-info":[{"award-number":["BMI\/11(48)\/2022"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["2021-11207"],"award-info":[{"award-number":["2021-11207"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biofilm"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.bioflm.2025.100265","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T11:13:30Z","timestamp":1740222810000},"page":"100265","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase inhibit biofilm formation by Pseudomonas aeruginosa CF2843 on three-dimensional aggregates of lung epithelial cells"],"prefix":"10.1016","volume":"9","author":[{"family":"Neetu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilpee","family":"Pal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srikrishna","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7109-2684","authenticated-orcid":false,"given":"T.N.C.","family":"Ramya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bioflm.2025.100265_bib1","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1183\/09031936.00228013","article-title":"CFTR: cystic fibrosis and beyond","volume":"44","author":"Mall","year":"2014","journal-title":"Eur Respir J"},{"key":"10.1016\/j.bioflm.2025.100265_bib2","doi-asserted-by":"crossref","first-page":"a009589","DOI":"10.1101\/cshperspect.a009589","article-title":"CFTR, mucins, and mucus obstruction in cystic fibrosis","volume":"2","author":"Kreda","year":"2012","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1016\/j.bioflm.2025.100265_bib3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/sj.gt.3300556","article-title":"The effect of mucolytic agents on gene transfer across a CF sputum barrier in vitro","volume":"5","author":"Stern","year":"1998","journal-title":"Gene Ther"},{"key":"10.1016\/j.bioflm.2025.100265_bib4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jcf.2004.10.002","article-title":"Prospective evaluation of emerging bacteria in cystic fibrosis","volume":"4","author":"Steinkamp","year":"2005","journal-title":"J Cyst Fibros"},{"key":"10.1016\/j.bioflm.2025.100265_bib5","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ijantimicag.2009.12.011","article-title":"Antibiotic resistance of bacterial biofilms","volume":"35","author":"Hoiby","year":"2010","journal-title":"Int J Antimicrob Agents"},{"key":"10.1016\/j.bioflm.2025.100265_bib6","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1128\/IAI.01282-06","article-title":"Molecular epidemiology and dynamics of Pseudomonas aeruginosa populations in lungs of cystic fibrosis patients","volume":"75","author":"Jelsbak","year":"2007","journal-title":"Infect Immun"},{"key":"10.1016\/j.bioflm.2025.100265_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0216401","article-title":"The anti-sigma factor MucA of Pseudomonas aeruginosa: dramatic differences of a mucA22 vs. a DeltamucA mutant in anaerobic acidified nitrite sensitivity of planktonic and biofilm bacteria in vitro and during chronic murine lung infection","volume":"14","author":"Panmanee","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.bioflm.2025.100265_bib8","doi-asserted-by":"crossref","first-page":"9691","DOI":"10.1038\/s41598-018-28009-5","article-title":"Viscoelastic properties of Pseudomonas aeruginosa variant biofilms","volume":"8","author":"Gloag","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.bioflm.2025.100265_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0082240","article-title":"Human host defense peptide LL-37 stimulates virulence factor production and adaptive resistance in Pseudomonas aeruginosa","volume":"8","author":"Strempel","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.bioflm.2025.100265_bib10","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1111\/1462-2920.12155","article-title":"The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin","volume":"15","author":"Tseng","year":"2013","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib11","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1111\/j.1365-2958.2009.06795.x","article-title":"Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production","volume":"73","author":"Byrd","year":"2009","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib12","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1038\/nature12155","article-title":"Psl trails guide exploration and microcolony formation in Pseudomonas aeruginosa biofilms","volume":"497","author":"Zhao","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.bioflm.2025.100265_bib13","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/s42003-022-03453-2","article-title":"The Pel polysaccharide is predominantly composed of a dimeric repeat of alpha-1,4 linked galactosamine and N-acetylgalactosamine","volume":"5","author":"Le Mauff","year":"2022","journal-title":"Commun Biol"},{"key":"10.1016\/j.bioflm.2025.100265_bib14","doi-asserted-by":"crossref","first-page":"11353","DOI":"10.1073\/pnas.1503058112","article-title":"Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix","volume":"112","author":"Jennings","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.bioflm.2025.100265_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000354","article-title":"Assembly and development of the Pseudomonas aeruginosa biofilm matrix","volume":"5","author":"Ma","year":"2009","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.bioflm.2025.100265_bib16","doi-asserted-by":"crossref","first-page":"5238","DOI":"10.1128\/AEM.00637-11","article-title":"Role of exopolysaccharides in Pseudomonas aeruginosa biofilm formation and architecture","volume":"77","author":"Ghafoor","year":"2011","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1574-695X.2012.00934.x","article-title":"The roles of biofilm matrix polysaccharide Psl in mucoid Pseudomonas aeruginosa biofilms","volume":"65","author":"Ma","year":"2012","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib18","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1111\/j.1462-2920.2011.02657.x","article-title":"The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix","volume":"14","author":"Colvin","year":"2012","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib19","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/s41522-021-00234-3","article-title":"The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis","volume":"7","author":"Morris","year":"2021","journal-title":"NPJ Biofilms Microbiomes"},{"key":"10.1016\/j.bioflm.2025.100265_bib20","first-page":"523","article-title":"Alginate-modifying enzymes: biological roles and biotechnological uses","volume":"6","author":"Ertesvag","year":"2015","journal-title":"Front Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib21","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/jac\/dkp165","article-title":"Importance of DNase and alginate lyase for enhancing free and liposome encapsulated aminoglycoside activity against Pseudomonas aeruginosa","volume":"64","author":"Alipour","year":"2009","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.bioflm.2025.100265_bib22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1600-0463.2006.apm_356.x","article-title":"Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms","volume":"114","author":"Alkawash","year":"2006","journal-title":"APMIS"},{"key":"10.1016\/j.bioflm.2025.100265_bib23","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jcf.2020.04.006","article-title":"Disruption of the extracellular polymeric network of Pseudomonas aeruginosa biofilms by alginate lyase enhances pathogen eradication by antibiotics","volume":"20","author":"Daboor","year":"2021","journal-title":"J Cyst Fibros"},{"key":"10.1016\/j.bioflm.2025.100265_bib24","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1038\/cr.2015.129","article-title":"PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix","volume":"25","author":"Yu","year":"2015","journal-title":"Cell Res"},{"key":"10.1016\/j.bioflm.2025.100265_bib25","doi-asserted-by":"crossref","first-page":"28374","DOI":"10.1074\/jbc.M115.674929","article-title":"Characterization of the Pseudomonas aeruginosa glycoside hydrolase PslG reveals that its levels are critical for psl polysaccharide biosynthesis and Biofilm Formation","volume":"290","author":"Baker","year":"2015","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bioflm.2025.100265_bib26","doi-asserted-by":"crossref","first-page":"7124","DOI":"10.1073\/pnas.1702798114","article-title":"Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity","volume":"114","author":"Snarr","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.bioflm.2025.100265_bib27","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1501632","article-title":"Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms","volume":"2","author":"Baker","year":"2016","journal-title":"Sci Adv"},{"issue":"7","key":"10.1016\/j.bioflm.2025.100265_bib28","article-title":"Glycoside hydrolase processing of the Pel polysaccharide alters biofilm biomechanics and Pseudomonas aeruginosa virulence","volume":"9","author":"Razvi","year":"2023","journal-title":"NPJ Biofilms Microbiomes"},{"key":"10.1016\/j.bioflm.2025.100265_bib29","doi-asserted-by":"crossref","first-page":"10760","DOI":"10.1074\/jbc.RA119.008511","article-title":"Molecular mechanism of Aspergillus fumigatus biofilm disruption by fungal and bacterial glycoside hydrolases","volume":"294","author":"Le Mauff","year":"2019","journal-title":"J Biol Chem"},{"key":"10.1016\/j.bioflm.2025.100265_bib30","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1002\/iub.2442","article-title":"Cellulophaga algicola alginate lyase inhibits biofilm formation of a clinical Pseudomonas aeruginosa strain MCC 2081","volume":"73","author":"Mahajan","year":"2021","journal-title":"IUBMB Life"},{"key":"10.1016\/j.bioflm.2025.100265_bib31","article-title":"Basic principles of cell culture","author":"Freshney","year":"2006","journal-title":"Culture of Cells and Tissue Engineering"},{"key":"10.1016\/j.bioflm.2025.100265_bib32","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1290\/0604027.1","article-title":"Life isn't flat: taking cancer biology to the next dimension","volume":"42","author":"Smalley","year":"2006","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"10.1016\/j.bioflm.2025.100265_bib33","doi-asserted-by":"crossref","DOI":"10.1038\/srep43321","article-title":"Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum","volume":"7","author":"Crabbe","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.bioflm.2025.100265_bib34","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/nrmicro2423","article-title":"Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions","volume":"8","author":"Barrila","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib35","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1128\/IAI.73.2.1129-1140.2005","article-title":"A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis","volume":"73","author":"Carterson","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.bioflm.2025.100265_bib36","doi-asserted-by":"crossref","first-page":"7106","DOI":"10.1128\/IAI.69.11.7106-7120.2001","article-title":"Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis","volume":"69","author":"Nickerson","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.bioflm.2025.100265_bib37","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1462-5822.2010.01548.x","article-title":"Alveolar epithelium protects macrophages from quorum sensing-induced cytotoxicity in a three-dimensional co-culture model","volume":"13","author":"Crabbe","year":"2011","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib38","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.micinf.2014.04.001","article-title":"The use of a three-dimensional cell culture model to investigate host-pathogen interactions of Francisella tularensis in human lung epithelial cells","volume":"16","author":"David","year":"2014","journal-title":"Microb Infect"},{"key":"10.1016\/j.bioflm.2025.100265_bib39","article-title":"Biofilm inhibitory effect of alginate lyases on mucoid P. aeruginosa from a cystic fibrosis patient","volume":"26","author":"Mahajan","year":"2021","journal-title":"Biochem Biophys Rep"},{"key":"10.1016\/j.bioflm.2025.100265_bib40","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","author":"Meier-Kolthoff","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.bioflm.2025.100265_bib41","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1097\/CCM.0b013e3182a120cd","article-title":"Pseudomonas aeruginosa virulence and antimicrobial resistance: two sides of the same coin?","volume":"42","author":"Hauser","year":"2014","journal-title":"Crit Care Med"},{"key":"10.1016\/j.bioflm.2025.100265_bib42","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1011221","article-title":"Current concepts on Pseudomonas aeruginosa interaction with human airway epithelium","volume":"19","author":"Muggeo","year":"2023","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.bioflm.2025.100265_bib43","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1378\/chest.11-0462","article-title":"Effect of antibiotic diversity on ventilator-associated pneumonia caused by ESKAPE Organisms","volume":"140","author":"Sandiumenge","year":"2011","journal-title":"Chest"},{"key":"10.1016\/j.bioflm.2025.100265_bib44","doi-asserted-by":"crossref","first-page":"680","DOI":"10.3390\/pathogens3030680","article-title":"Pseudomonas aeruginosa diversification during infection development in cystic fibrosis lungs-A review","volume":"3","author":"Sousa","year":"2014","journal-title":"Pathogens"},{"key":"10.1016\/j.bioflm.2025.100265_bib45","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1309\/KJRPC7DD5TR9NTDM","article-title":"Mucoid Pseudomonas in cystic fibrosis","volume":"128","author":"Pritt","year":"2007","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.bioflm.2025.100265_bib46","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/1716087","article-title":"Prevalence of algD, pslD, pelF, ppgl, and PAPI-1 genes involved in Biofilm Formation in clinical Pseudomonas aeruginosa strains","author":"Rajabi","year":"2022","journal-title":"BioMed Res Int"},{"key":"10.1016\/j.bioflm.2025.100265_bib47","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jab.2014.05.002","article-title":"The prevalence of some Pseudomonas virulence genes related to biofilm formation and alginate production among clinical isolates","volume":"13","author":"Ghadaksaz","year":"2015","journal-title":"J Appl Biomed"},{"key":"10.1016\/j.bioflm.2025.100265_bib48","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/j.1365-2958.2005.04552.x","article-title":"Understanding the control of Pseudomonas aeruginosa alginate synthesis and the prospects for management of chronic infections in cystic fibrosis","volume":"56","author":"Ramsey","year":"2005","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib49","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1046\/j.1365-2958.2003.03877.x","article-title":"Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms","volume":"51","author":"Friedman","year":"2004","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib50","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1099\/mic.0.27410-0","article-title":"The pel genes of the Pseudomonas aeruginosa PAK strain are involved at early and late stages of biofilm formation","volume":"151","author":"Vasseur","year":"2005","journal-title":"Microbiology (Read)"},{"key":"10.1016\/j.bioflm.2025.100265_bib51","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24108709","article-title":"How three self-secreted biofilm exopolysaccharides of Pseudomonas aeruginosa, psl, pel, and alginate, can each Be exploited for antibiotic adjuvant effects in cystic fibrosis lung infection","volume":"24","author":"Chung","year":"2023","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.bioflm.2025.100265_bib52","doi-asserted-by":"crossref","DOI":"10.1128\/aac.00052-22","article-title":"Preclinical evaluation of recombinant microbial glycoside hydrolases as antibiofilm agents in acute pulmonary Pseudomonas aeruginosa infection","volume":"66","author":"Ostapska","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.bioflm.2025.100265_bib53","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00234-19","article-title":"Treatment with the Pseudomonas aeruginosa glycoside hydrolase PslG combats wound infection by improving antibiotic efficacy and host innate immune activity","volume":"63","author":"Pestrak","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.bioflm.2025.100265_bib54","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2020.116625","article-title":"Glycoside hydrolase (PelA(h)) immobilization prevents Pseudomonas aeruginosa biofilm formation on cellulose-based wound dressing","volume":"246","author":"Szymanska","year":"2020","journal-title":"Carbohydr Polym"},{"key":"10.1016\/j.bioflm.2025.100265_bib55","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1021\/acs.langmuir.9b02188","article-title":"Specific disruption of established Pseudomonas aeruginosa biofilms using polymer-attacking enzymes","volume":"36","author":"Kovach","year":"2020","journal-title":"Langmuir"},{"key":"10.1016\/j.bioflm.2025.100265_bib56","series-title":"Therapeutic medicine for cystic fibrosis","author":"Sakaguchi","year":"1996"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100265_bib57","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11274-005-7713-4","article-title":"A novel alginate lyase with high activity on acetylated alginate of Pseudomonas aeruginosa FRD1 from Pseudomonas sp. QD03","volume":"22","author":"Xiao","year":"2006","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1016\/j.bioflm.2025.100265_bib58","doi-asserted-by":"crossref","first-page":"9390","DOI":"10.1038\/s41598-020-66293-2","article-title":"Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms","volume":"10","author":"Blanco-Cabra","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.bioflm.2025.100265_bib59","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1128\/aem.60.7.2355-2359.1994","article-title":"Role of alginate lyase in cell detachment of Pseudomonas aeruginosa","volume":"60","author":"Boyd","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib60","doi-asserted-by":"crossref","first-page":"696","DOI":"10.3390\/ph8040696","article-title":"Controlling persister and biofilm cells of gram-negative bacteria with a new 1,3,5-triazine derivative","volume":"8","author":"Bahar","year":"2015","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.bioflm.2025.100265_bib61","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1128\/AAC.01789-12","article-title":"Alginate lyase exhibits catalysis-independent biofilm dispersion and antibiotic synergy","volume":"57","author":"Lamppa","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.bioflm.2025.100265_bib62","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1038\/s41587-022-01584-9","article-title":"Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms","volume":"41","author":"Mazzolini","year":"2023","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.bioflm.2025.100265_bib63","doi-asserted-by":"crossref","first-page":"6796","DOI":"10.1073\/pnas.102171199","article-title":"Clara cell impact in air-side activation of CFTR in small pulmonary airways","volume":"99","author":"Kulaksiz","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.bioflm.2025.100265_bib64","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1006\/bbrc.1996.5939","article-title":"CFTR-like chloride channels in non-ciliated bronchiolar epithelial (Clara) cells","volume":"230","author":"Chinet","year":"1997","journal-title":"Biochem Biophys Res Commun"},{"issue":"Pt 3","key":"10.1016\/j.bioflm.2025.100265_bib65","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1113\/jphysiol.1997.sp021960","article-title":"Chloride secretion in the trachea of null cystic fibrosis mice: the effects of transfection with pTrial10-CFTR2","volume":"499","author":"MacVinish","year":"1997","journal-title":"J Physiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib66","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1126\/science.1163518","article-title":"TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity","volume":"322","author":"Caputo","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.bioflm.2025.100265_bib67","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1038\/nature07313","article-title":"TMEM16A confers receptor-activated calcium-dependent chloride conductance","volume":"455","author":"Yang","year":"2008","journal-title":"Nature"},{"issue":"Suppl 2","key":"10.1016\/j.bioflm.2025.100265_bib68","doi-asserted-by":"crossref","first-page":"S152","DOI":"10.1016\/S1569-1993(11)60020-9","article-title":"Mouse models of cystic fibrosis: phenotypic analysis and research applications","volume":"10","author":"Wilke","year":"2011","journal-title":"J Cyst Fibros"},{"key":"10.1016\/j.bioflm.2025.100265_bib69","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1016\/j.jcf.2017.09.001","article-title":"Animal and model systems for studying cystic fibrosis","volume":"17","author":"Rosen","year":"2018","journal-title":"J Cyst Fibros"},{"key":"10.1016\/j.bioflm.2025.100265_bib70","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.3389\/fphar.2018.01475","article-title":"Animal models in the pathophysiology of cystic fibrosis","volume":"9","author":"Semaniakou","year":"2018","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.bioflm.2025.100265_bib71","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.ajpath.2020.10.017","article-title":"Animal and cell culture models for cystic fibrosis: which model is right for your application?","volume":"191","author":"McCarron","year":"2021","journal-title":"Am J Pathol"},{"key":"10.1016\/j.bioflm.2025.100265_bib72","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1172\/JCI60610","article-title":"Abnormal endocrine pancreas function at birth in cystic fibrosis ferrets","volume":"122","author":"Olivier","year":"2012","journal-title":"J Clin Investig"},{"key":"10.1016\/j.bioflm.2025.100265_bib73","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aau7531","article-title":"In utero and postnatal VX-770 administration rescues multiorgan disease in a ferret model of cystic fibrosis","volume":"11","author":"Sun","year":"2019","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.bioflm.2025.100265_bib74","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.ajpath.2014.01.035","article-title":"Gastrointestinal pathology in juvenile and adult CFTR-knockout ferrets","volume":"184","author":"Sun","year":"2014","journal-title":"Am J Pathol"},{"key":"10.1016\/j.bioflm.2025.100265_bib75","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00282-18","article-title":"Modeling host-pathogen interactions in the context of the microenvironment: three-dimensional cell culture comes of age","volume":"86","author":"Barrila","year":"2018","journal-title":"Infect Immun"},{"key":"10.1016\/j.bioflm.2025.100265_bib76","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1161\/CIRCRESAHA.108.175331","article-title":"Mechanical control of tissue morphogenesis","volume":"103","author":"Patwari","year":"2008","journal-title":"Circ Res"},{"key":"10.1016\/j.bioflm.2025.100265_bib77","doi-asserted-by":"crossref","first-page":"8871","DOI":"10.1038\/ncomms9871","article-title":"Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6","volume":"6","author":"Miura","year":"2015","journal-title":"Nat Commun"},{"key":"10.1016\/j.bioflm.2025.100265_bib78","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1111\/j.1462-2920.2004.00605.x","article-title":"Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins","volume":"6","author":"Lambertsen","year":"2004","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib79","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/s41587-019-0217-9","article-title":"Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome","volume":"37","author":"Wenger","year":"2019","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.bioflm.2025.100265_bib80","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1186\/s13059-021-02483-z","article-title":"Trycycler: consensus long-read assemblies for bacterial genomes","volume":"22","author":"Wick","year":"2021","journal-title":"Genome Biol"},{"key":"10.1016\/j.bioflm.2025.100265_bib81","article-title":"Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification","volume":"7","author":"Schwengers","year":"2021","journal-title":"Microb Genom"},{"key":"10.1016\/j.bioflm.2025.100265_bib82","doi-asserted-by":"crossref","first-page":"D646","DOI":"10.1093\/nar\/gkv1227","article-title":"Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database","volume":"44","author":"Winsor","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bioflm.2025.100265_bib83","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1186\/1471-2105-11-595","article-title":"Scalable analysis of bacterial genome variation at the population level","volume":"11","author":"Jolley","year":"2010","journal-title":"BMC Bioinf"},{"key":"10.1016\/j.bioflm.2025.100265_bib84","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1128\/JCM.06094-11","article-title":"Multilocus sequence typing of total-genome-sequenced bacteria","volume":"50","author":"Larsen","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.bioflm.2025.100265_bib85","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/1755-0998.13096","article-title":"PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies","volume":"20","author":"Zhang","year":"2020","journal-title":"Mol Ecol Resour"},{"key":"10.1016\/j.bioflm.2025.100265_bib86","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.bioflm.2025.100265_bib87","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1186\/s13059-014-0524-x","article-title":"The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes","volume":"15","author":"Treangen","year":"2014","journal-title":"Genome Biol"},{"key":"10.1016\/j.bioflm.2025.100265_bib88","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bioflm.2025.100265_bib89","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-91456-0","article-title":"AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence","volume":"11","author":"Feldgarden","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.bioflm.2025.100265_bib90","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40793-022-00413-5","article-title":"Distinctive signatures of pathogenic and antibiotic resistant potentials in the hadal microbiome","volume":"17","author":"He","year":"2022","journal-title":"Environ Microbiome"},{"key":"10.1016\/j.bioflm.2025.100265_bib91","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: in-depth characterization and visualization of bacterial genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.bioflm.2025.100265_bib92","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1017\/S1551929500062404","article-title":"The use of poly-L-lysine as an adhesive in scanning electron microscopy","volume":"16","author":"Taylor","year":"2008","journal-title":"Microscopy Today"},{"key":"10.1016\/j.bioflm.2025.100265_bib93","article-title":"Scanning electron microscopy","volume":"2","author":"Fischer","year":"2012","journal-title":"Curr Protoc Microbiol Chapter"},{"key":"10.1016\/j.bioflm.2025.100265_bib94","doi-asserted-by":"crossref","first-page":"12342","DOI":"10.1021\/acs.analchem.7b04293","article-title":"Mining high-complexity motifs in glycans: a new language to uncover the fine specificities of lectins and glycosidases","volume":"89","author":"Klamer","year":"2017","journal-title":"Anal Chem"}],"container-title":["Biofilm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207525000139?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207525000139?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T20:58:47Z","timestamp":1762635527000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590207525000139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":94,"alternative-id":["S2590207525000139"],"URL":"https:\/\/doi.org\/10.1016\/j.bioflm.2025.100265","relation":{},"ISSN":["2590-2075"],"issn-type":[{"value":"2590-2075","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase inhibit biofilm formation by Pseudomonas aeruginosa CF2843 on three-dimensional aggregates of lung epithelial cells","name":"articletitle","label":"Article Title"},{"value":"Biofilm","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioflm.2025.100265","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"100265"}}