{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:22:21Z","timestamp":1790882541177,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014419","name":"EIT Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014419","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010686","name":"H2020 European Institute of Innovation and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010686","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2021-02091"],"award-info":[{"award-number":["2021-02091"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biofilm"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.bioflm.2025.100311","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T06:58:31Z","timestamp":1754549911000},"page":"100311","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement"],"prefix":"10.1016","volume":"10","author":[{"given":"Linglu","family":"Hong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Hjort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan I.","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6663-6536","authenticated-orcid":false,"given":"Cecilia","family":"Persson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.bioflm.2025.100311_bib1","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1002\/pi.6136","article-title":"Poly(Methyl methacrylate) bone cement, its rise, growth, downfall and future","volume":"70","author":"Soleymani Eil Bakhtiari","year":"2021","journal-title":"Polym Int"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib2","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1302\/0301-620X.42B1.28","article-title":"Anchorage of the femoral head prosthesis to the shaft of the femur","volume":"42 B","author":"Charnley","year":"1960","journal-title":"J Bone Joint Surg"},{"issue":"3","key":"10.1016\/j.bioflm.2025.100311_bib3","first-page":"518","article-title":"The bonding of prostheses to bone by cement","volume":"46","author":"Charnley","year":"1964","journal-title":"Clin Orthop Relat Res"},{"issue":"2","key":"10.1016\/j.bioflm.2025.100311_bib4","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/jbm.b.30398","article-title":"Injectable bone cements for use in vertebroplasty and kyphoplasty: state-Of-the-Art review","volume":"76","author":"Lewis","year":"2006","journal-title":"J Biomed Mater Res B Appl Biomater"},{"issue":"5","key":"10.1016\/j.bioflm.2025.100311_bib5","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3340\/jkns.2012.52.5.435","article-title":"Effect of bone cement volume and stiffness on occurrences of adjacent vertebral fractures after vertebroplasty","volume":"52","author":"Kim","year":"2012","journal-title":"J Korean Neurosurg Soc"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-018-3486-x","article-title":"Surgical outcomes of infectious spondylitis after vertebroplasty, and comparisons between pyogenic and tuberculosis","volume":"18","author":"Liao","year":"2018","journal-title":"BMC Infect Dis"},{"issue":"4","key":"10.1016\/j.bioflm.2025.100311_bib7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.4055\/cios.2010.2.4.203","article-title":"Revision surgery after vertebroplasty or kyphoplasty","volume":"2","author":"Ha","year":"2010","journal-title":"Clinics in orthopedic surgery"},{"issue":"4","key":"10.1016\/j.bioflm.2025.100311_bib8","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4184\/asj.2013.7.4.294","article-title":"Causes of late revision surgery after bone cement augmentation in osteoporotic vertebral compression fractures","volume":"7","author":"Ha","year":"2013","journal-title":"Asian Spine J"},{"issue":"6","key":"10.1016\/j.bioflm.2025.100311_bib9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3171\/foc.2004.17.6.6","article-title":"Infected vertebroplasty. Report of two cases and review of the literature","volume":"17","author":"Walker","year":"2004","journal-title":"Neurosurg Focus"},{"issue":"7","key":"10.1016\/j.bioflm.2025.100311_bib10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41579-018-0019-y","article-title":"Implant infections: adhesion, biofilm formation and immune evasion","volume":"16","author":"Arciola","year":"2018","journal-title":"Nat Rev Microbiol"},{"issue":"12","key":"10.1016\/j.bioflm.2025.100311_bib11","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.3390\/jcm11123481","article-title":"Antibiotic cement utilization for the prophylaxis and treatment of infections in spine surgery: basic science principles and rationale for clinical use","volume":"11","author":"Anderson","year":"2022","journal-title":"J Clin Med"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib12","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/s13018-019-1200-3","article-title":"Properties and clinical application safety of antibiotic-loaded bone cement in kyphoplasty","volume":"14","author":"Opalko","year":"2019","journal-title":"J Orthop Surg Res"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib13","first-page":"826","article-title":"Current status of percutaneous vertebroplasty and percutaneous kyphoplasty - a review","volume":"19","author":"Yang","year":"2013","journal-title":"Med Sci Monit"},{"issue":"12","key":"10.1016\/j.bioflm.2025.100311_bib14","doi-asserted-by":"crossref","first-page":"3258","DOI":"10.1016\/j.jbiomech.2015.06.026","article-title":"Biomechanics of low-modulus and standard acrylic bone cements in simulated vertebroplasty: a human Ex vivo study","volume":"48","author":"Holub","year":"2015","journal-title":"J Biomech"},{"key":"10.1016\/j.bioflm.2025.100311_bib15","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jmbbm.2014.01.002","article-title":"Compressive mechanical properties and cytocompatibility of bone-compliant, linoleic acid-modified bone cement in a bovine model","volume":"32","author":"L\u00f3pez","year":"2014","journal-title":"J Mech Behav Biomed Mater"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3390\/jfb12010005","article-title":"Functional properties of low-modulus PMMA bone cements containing linoleic acid","volume":"12","author":"Robo","year":"2021","journal-title":"J Funct Biomater"},{"issue":"3","key":"10.1016\/j.bioflm.2025.100311_bib17","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1177\/0885328215581316","article-title":"The effect of unsaturated fatty acid and triglyceride oil addition on the mechanical and antibacterial properties of acrylic bone cements","volume":"30","author":"Persson","year":"2015","journal-title":"J Biomater Appl"},{"key":"10.1016\/j.bioflm.2025.100311_bib18","first-page":"1","article-title":"In vitro and in vivo response to low-modulus PMMA-based bone cement","author":"Carlsson","year":"2015","journal-title":"BioMed Research International 2015"},{"key":"10.1016\/j.bioflm.2025.100311_bib20","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.actbio.2018.03.014","article-title":"In vivo response to a low-modulus PMMA bone cement in an ovine model","volume":"72","author":"Robo","year":"2018","journal-title":"Acta Biomater"},{"issue":"11","key":"10.1016\/j.bioflm.2025.100311_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/antibiotics9110788","article-title":"Unsaturated fatty acids control biofilm formation of Staphylococcus aureus and other gram-positive bacteria","volume":"9","author":"Yuyama","year":"2020","journal-title":"Antibiotics"},{"issue":"23","key":"10.1016\/j.bioflm.2025.100311_bib22","doi-asserted-by":"crossref","first-page":"5157","DOI":"10.1016\/j.febslet.2005.08.028","article-title":"Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids","volume":"579","author":"Zheng","year":"2005","journal-title":"FEBS (Fed Eur Biochem Soc) Lett"},{"issue":"2","key":"10.1016\/j.bioflm.2025.100311_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/spectrum.02767-21","article-title":"Host polyunsaturated fatty acids potentiate aminoglycoside killing of Staphylococcus aureus","volume":"10","author":"Beavers","year":"2022","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.bioflm.2025.100311_bib24","series-title":"Biological macromolecules","first-page":"165","article-title":"Biological macromolecules as antimicrobial agents","author":"Shahruzzaman","year":"2022"},{"issue":"25","key":"10.1016\/j.bioflm.2025.100311_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/app.52409","article-title":"Defining the role of linoleic acid in acrylic bone cement","volume":"139","author":"Ayyachi","year":"2022","journal-title":"J Appl Polym Sci"},{"key":"10.1016\/j.bioflm.2025.100311_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmbbm.2021.104437","article-title":"Long-term mechanical properties of a novel low-modulus bone cement for the treatment of osteoporotic vertebral compression fractures","volume":"118","author":"Robo","year":"2021","journal-title":"J Mech Behav Biomed Mater"},{"issue":"6","key":"10.1016\/j.bioflm.2025.100311_bib27","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1002\/jbm.820120602","article-title":"Effect of simplex liquid methyl methacrylate monomer on cells","volume":"12","author":"Welch","year":"1978","journal-title":"J Biomed Mater Res"},{"key":"10.1016\/j.bioflm.2025.100311_bib28","series-title":"Orthopaedic bone cements","first-page":"313","article-title":"Antibiotic-loaded bone cements","author":"Deb","year":"2008"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib29","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1186\/s12879-023-08471-x","article-title":"Trends in microbiological epidemiology of orthopedic infections: a large retrospective study from 2008 to 2021","volume":"23","author":"Wang","year":"2023","journal-title":"BMC Infect Dis"},{"issue":"7","key":"10.1016\/j.bioflm.2025.100311_bib30","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1128\/iai.65.7.2812-2820.1997","article-title":"Pathogenicity island sequences of pyelonephritogenic Escherichia coli CFT073 are associated with virulent uropathogenic strains","volume":"65","author":"Kao","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.bioflm.2025.100311_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2021.802303","article-title":"Modular 3D-Printed peg bio film device for flexible setup of surface-related biofilm studies","volume":"11","author":"Zaborskyte","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.bioflm.2025.100311_bib32","series-title":"Standard specification for acrylic bone cement. Standard specification for acrylic bone cement","first-page":"1","year":"2021"},{"issue":"July","key":"10.1016\/j.bioflm.2025.100311_bib33","article-title":"New and simplified method for drug combination studies by checkerboard assay","volume":"8","author":"Bellio","year":"2021","journal-title":"MethodsX"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jac\/dkg301","article-title":"Synergy, antagonism, and what the chequerboard puts between them","volume":"52","author":"Odds","year":"2003","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib35","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.biori.2018.12.001","article-title":"Checkerboard testing method indicates synergic effect of pelgipeptins against multidrug resistant Klebsiella pneumoniae","volume":"3","author":"Costa","year":"2019","journal-title":"Biotechnol Res Innovat"},{"key":"10.1016\/j.bioflm.2025.100311_bib36","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.msec.2016.01.048","article-title":"Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements","volume":"62","author":"Hoess","year":"2016","journal-title":"Mater Sci Eng C"},{"key":"10.1016\/j.bioflm.2025.100311_bib37","article-title":"Bone-compliant cements for vertebral augmentation","author":"Robo","year":"2018","journal-title":"Acta Univ Upsal"},{"key":"10.1016\/j.bioflm.2025.100311_bib38","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.foodcont.2017.04.035","article-title":"Supercritical fluid extracts of Moringa oleifera and their unsaturated fatty acid components inhibit biofilm formation by Staphylococcus aureus","volume":"80","author":"Lee","year":"2017","journal-title":"Food Control"},{"issue":"3","key":"10.1016\/j.bioflm.2025.100311_bib39","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1177\/0885328215581316","article-title":"The effect of unsaturated fatty acid and triglyceride oil addition on the mechanical and antibacterial properties of acrylic bone cements","volume":"30","author":"Persson","year":"2015","journal-title":"J Biomater Appl"},{"key":"10.1016\/j.bioflm.2025.100311_bib40","series-title":"The well-cemented total hip arthroplasty: theory and practice","first-page":"93","article-title":"Properties of bone cement : the three interfaces","author":"Draenert","year":"2005"},{"issue":"8","key":"10.1016\/j.bioflm.2025.100311_bib41","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1002\/jbm.b.33488","article-title":"A novel liposomal drug delivery system for PMMA bone cements","volume":"104","author":"Ayre","year":"2016","journal-title":"J Biomed Mater Res B Appl Biomater"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib42","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1099\/00221287-115-1-233","article-title":"Mechanism of the inhibitory action of linoleic acid on the growth of Staphylococcus aureus","volume":"115","author":"Greenway","year":"1979","journal-title":"J Gen Microbiol"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib43","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/BF02633110","article-title":"Solubility of linoleic acid in aqueous solutions and its reaction with water","volume":"38","author":"Mabrouk","year":"1961","journal-title":"J Americ Oil Chem Soc"},{"issue":"6","key":"10.1016\/j.bioflm.2025.100311_bib44","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1080\/000164700317362280","article-title":"Gentamicin release from polymethylmethacrylate bone cements and Staphylococcus aureus biofilm formation","volume":"71","author":"van de Belt","year":"2000","journal-title":"Acta Orthop Scand"},{"key":"10.1016\/j.bioflm.2025.100311_bib45","series-title":"Selected topics in plastic reconstructive surgery","year":"2012"},{"key":"10.1016\/j.bioflm.2025.100311_bib46","doi-asserted-by":"crossref","DOI":"10.5402\/2011\/290851","article-title":"Antibiotic-loaded cement in orthopedic surgery: a review","author":"Bistolfi","year":"2011","journal-title":"ISRN Orthop 2011"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib47","doi-asserted-by":"crossref","first-page":"38","DOI":"10.5435\/00124635-200301000-00006","article-title":"Use of antibiotic-impregnated cement in total joint arthroplasty","volume":"11","author":"Joseph","year":"2003","journal-title":"J Am Acad Orthop Surg"},{"issue":"1","key":"10.1016\/j.bioflm.2025.100311_bib48","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/jphp.12315","article-title":"Combating against methicillin-resistant Staphylococcus aureus - two fatty acids from purslane (Portulaca Oleracea L.) exhibit synergistic effects with erythromycin","volume":"67","author":"Chan","year":"2015","journal-title":"J Pharm Pharmacol"},{"issue":"May","key":"10.1016\/j.bioflm.2025.100311_bib49","first-page":"1","article-title":"Fatty acids as aminoglycoside antibiotic adjuvants against Staphylococcus Aureus","volume":"13","author":"Park","year":"2022","journal-title":"Front Microbiol"},{"issue":"23","key":"10.1016\/j.bioflm.2025.100311_bib50","doi-asserted-by":"crossref","first-page":"3963","DOI":"10.3390\/ma12233963","article-title":"Effect of physiological fluids contamination on selected mechanical properties of acrylate bone cement","volume":"12","author":"Karpi\u0144ski","year":"2019","journal-title":"Materials"}],"container-title":["Biofilm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207525000590?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207525000590?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T20:56:36Z","timestamp":1772916996000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590207525000590"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":49,"alternative-id":["S2590207525000590"],"URL":"https:\/\/doi.org\/10.1016\/j.bioflm.2025.100311","relation":{},"ISSN":["2590-2075"],"issn-type":[{"value":"2590-2075","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement","name":"articletitle","label":"Article Title"},{"value":"Biofilm","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioflm.2025.100311","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"100311"}}