{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T21:06:08Z","timestamp":1793653568071,"version":"4.5.1"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021178","name":"University of Nottingham Ningbo China","doi-asserted-by":"publisher","award":["I01220800007"],"award-info":[{"award-number":["I01220800007"]}],"id":[{"id":"10.13039\/501100021178","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biofilm"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.bioflm.2026.100354","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T08:07:41Z","timestamp":1770710861000},"page":"100354","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Bioelectrochemical detection of viable but non-culturable bacterial cells on screen-printed carbon electrodes"],"prefix":"10.1016","volume":"11","author":[{"given":"Opeyemi Temitope","family":"Otemoye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia","family":"Nawab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifeng","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Mattedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Debiagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loh","family":"Teng-Hern Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim R.","family":"Hardie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenbo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-1564","authenticated-orcid":false,"given":"Enrico","family":"Marsili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.bioflm.2026.100354_bib1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.4014\/jmb.1310.10013","article-title":"Advances in rapid detection methods for foodborne pathogens","volume":"24","author":"Oh","year":"2014","journal-title":"J Microbiol Biotechnol"},{"issue":"6","key":"10.1016\/j.bioflm.2026.100354_bib2","doi-asserted-by":"crossref","first-page":"3106","DOI":"10.1111\/1541-4337.12618","article-title":"Microbial detection and identification methods: Bench top assays to omics approaches","volume":"19","author":"Ferone","year":"2020","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"10.1016\/j.bioflm.2026.100354_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.818468","article-title":"Assessing viability and stress tolerance of probiotics\u2014A review","volume":"12","author":"Wendel","year":"2022","journal-title":"Front Microbiol"},{"issue":"10","key":"10.1016\/j.bioflm.2026.100354_bib4","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.tim.2023.04.009","article-title":"Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy","volume":"31","author":"Liu","year":"2023","journal-title":"Trends Microbiol"},{"key":"10.1016\/j.bioflm.2026.100354_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2014.00258","article-title":"The importance of the viable but non-culturable state in human bacterial pathogens","volume":"5","author":"Li","year":"2014","journal-title":"Front Microbiol"},{"issue":"7","key":"10.1016\/j.bioflm.2026.100354_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22073541","article-title":"Detecting bacterial cell viability in few \u03bcL solutions from impedance measurements on silicon-based biochips","volume":"22","author":"Bhat","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"15","key":"10.1016\/j.bioflm.2026.100354_bib7","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1128\/AEM.02932-09","article-title":"Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry","volume":"76","author":"Khan","year":"2010","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib8","article-title":"Characterization and potential mechanisms of highly antibiotic tolerant VBNC Escherichia coli induced by low level chlorination","volume":"10","author":"Ye","year":"2020","journal-title":"Sci Rep"},{"issue":"2","key":"10.1016\/j.bioflm.2026.100354_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00540-18","article-title":"Viable-but-Nonculturable Listeria monocytogenes and Salmonella enterica serovar Thompson induced by chlorine stress remain infectious","volume":"9","author":"Highmore","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.bioflm.2026.100354_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.lwt.2021.112623","article-title":"A novel procedure in combination of genomic sequencing, flow cytometry and routine culturing for confirmation of beer spoilage caused by Pediococcus damnosus in viable but nonculturable state","volume":"154","author":"Xu","year":"2022","journal-title":"Lwt"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib11","article-title":"Discovery and control of culturable and viable but non-culturable cells of a distinctive Lactobacillus harbinensis strain from spoiled beer","volume":"8","author":"Liu","year":"2018","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib12","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3109\/1040841X.2013.794127","article-title":"Thirty years of viable but nonculturable state research: unsolved molecular mechanisms","volume":"41","author":"Pinto","year":"2013","journal-title":"Crit Rev Microbiol"},{"issue":"7","key":"10.1016\/j.bioflm.2026.100354_bib13","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1080\/07388551.2022.2095253","article-title":"Advanced diagnostic methods for identification of bacterial foodborne pathogens: contemporary and upcoming challenges","volume":"43","author":"Panwar","year":"2022","journal-title":"Crit Rev Biotechnol"},{"issue":"22","key":"10.1016\/j.bioflm.2026.100354_bib14","doi-asserted-by":"crossref","first-page":"6461","DOI":"10.1039\/C9AN01747J","article-title":"Electrochemical sensors for rapid diagnosis of pathogens in real time","volume":"144","author":"Simoska","year":"2019","journal-title":"Analyst"},{"key":"10.1016\/j.bioflm.2026.100354_bib15","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1016\/j.snb.2017.08.179","article-title":"Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy","volume":"255","author":"Li","year":"2018","journal-title":"Sensor Actuator B Chem"},{"key":"10.1016\/j.bioflm.2026.100354_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115674","article-title":"Toll-like receptor-immobilized carbon paste electrodes with plasma functionalized amine termination: towards real-time electrochemical based triaging of gram-negative bacteria","volume":"241","author":"Gangwar","year":"2023","journal-title":"Biosens Bioelectron"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s42823-023-00518-y","article-title":"An electrochemical sensor based on graphene-chitosan-cyclodextrin modification for the detection of Staphylococcus aureus","volume":"34","author":"Li","year":"2023","journal-title":"Carbon Lett"},{"issue":"5","key":"10.1016\/j.bioflm.2026.100354_bib18","doi-asserted-by":"crossref","first-page":"5637","DOI":"10.1021\/acsami.3c17151","article-title":"A wireless, CRISPR-polymer dot electrochemical sensor for the diagnosis of bacterial pneumonia and multi-drug resistance","volume":"16","author":"Im","year":"2024","journal-title":"ACS Appl Mater Interfaces"},{"issue":"23","key":"10.1016\/j.bioflm.2026.100354_bib19","doi-asserted-by":"crossref","first-page":"7329","DOI":"10.1128\/AEM.00177-08","article-title":"Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms","volume":"74","author":"Marsili","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.bioflm.2026.100354_bib20","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.biortech.2018.02.073","article-title":"Weak electricigens: a new avenue for bioelectrochemical research","volume":"258","author":"Doyle","year":"2018","journal-title":"Bioresour Technol"},{"issue":"5","key":"10.1016\/j.bioflm.2026.100354_bib21","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.tibtech.2021.10.002","article-title":"Capturing the signal of weak electricigens: a worthy endeavour","volume":"40","author":"Aiyer","year":"2022","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.bioflm.2026.100354_bib22","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.biortech.2013.02.069","article-title":"Effect of shear rate on the response of microbial fuel cell toxicity sensor to Cu(II)","volume":"136","author":"Shen","year":"2013","journal-title":"Bioresour Technol"},{"issue":"3","key":"10.1016\/j.bioflm.2026.100354_bib23","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.bjm.2017.02.001","article-title":"Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate","volume":"48","author":"Dong","year":"2017","journal-title":"Braz J Microbiol"},{"issue":"5","key":"10.1016\/j.bioflm.2026.100354_bib24","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1134\/S0003683824605031","article-title":"Development of a bio-selecting agent based on immobilized bacterial cells with amidase activity for bio-detection of acrylamide","volume":"60","author":"Protasova","year":"2024","journal-title":"Appl Biochem Microbiol"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib25","doi-asserted-by":"crossref","DOI":"10.1007\/s11274-018-2584-7","article-title":"Immobilization pattern of morphologically different microorganisms on bacterial cellulose membranes","volume":"35","author":"\u017bywicka","year":"2019","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1016\/j.bioflm.2026.100354_bib26","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.cej.2019.03.208","article-title":"Au(III) adsorption and reduction to gold particles on cost-effective tannin acid immobilized dialdehyde corn starch","volume":"370","author":"Liu","year":"2019","journal-title":"Chem Eng J"},{"key":"10.1016\/j.bioflm.2026.100354_bib27","article-title":"A novel method for immobilizing anammox bacteria in polyurethane foam carriers through dewatering","volume":"53","author":"Zheng","year":"2023","journal-title":"J Water Proc Eng"},{"issue":"166","key":"10.1016\/j.bioflm.2026.100354_bib28","doi-asserted-by":"crossref","DOI":"10.1098\/rsif.2020.0013","article-title":"Bioelectrical understanding and engineering of cell biology","volume":"17","author":"Schofield","year":"2020","journal-title":"J R Soc Interface"},{"key":"10.1016\/j.bioflm.2026.100354_bib29","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.watres.2018.05.055","article-title":"Induction of Escherichia coli into a VBNC state through chlorination\/chloramination and differences in characteristics of the bacterium between states","volume":"142","author":"Chen","year":"2018","journal-title":"Water Res"},{"key":"10.1016\/j.bioflm.2026.100354_bib30","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.elecom.2018.05.002","article-title":"Highly activated screen-printed carbon electrodes by electrochemical treatment with hydrogen peroxide","volume":"91","author":"Gonz\u00e1lez-S\u00e1nchez","year":"2018","journal-title":"Electrochem Commun"},{"issue":"3","key":"10.1016\/j.bioflm.2026.100354_bib31","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1111\/jam.15325","article-title":"Assessment of physiological responses of bacteria to chlorine and UV disinfection using a plate count method, flow cytometry and viability PCR","volume":"132","author":"Chiang","year":"2022","journal-title":"J Appl Microbiol"},{"key":"10.1016\/j.bioflm.2026.100354_bib32","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.seares.2014.07.003","article-title":"Ecology of intertidal microbial biofilms: mechanisms, patterns and future research needs","volume":"92","author":"Van Colen","year":"2014","journal-title":"J Sea Res"},{"key":"10.1016\/j.bioflm.2026.100354_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2024.110889","article-title":"Development, evaluation and application of propidium monoazide (PMA) based methodologies on viable cell quantification of Pediococcus acidilactici in rice noodles products","volume":"168","author":"Xu","year":"2025","journal-title":"Food Control"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-018-3612-9","article-title":"Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study","volume":"18","author":"Mangiaterra","year":"2018","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.bioflm.2026.100354_bib35","article-title":"Methodology to metabolically inactivate bacteria for Caenorhabditis elegans research","volume":"197","author":"Beydoun","year":"2023","journal-title":"JoVE J"},{"key":"10.1016\/j.bioflm.2026.100354_bib36","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.jes.2017.07.006","article-title":"Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli: culturability, membrane permeability, metabolism, and genetic damage","volume":"65","author":"Xu","year":"2018","journal-title":"J Environ Sci"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib37","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.mimet.2007.03.008","article-title":"Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target","volume":"70","author":"Lavenir","year":"2007","journal-title":"J Microbiol Methods"},{"issue":"2","key":"10.1016\/j.bioflm.2026.100354_bib38","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.mimet.2011.04.018","article-title":"Viable but non-culturable (VBNC) bacteria: gene expression in planktonic and biofilm cells","volume":"86","author":"Trevors","year":"2011","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.bioflm.2026.100354_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.ese.2020.100053","article-title":"Immobilisation of electrochemically active bacteria on screen-printed electrodes for rapid in situ toxicity biosensing","volume":"3","author":"Uria","year":"2020","journal-title":"Environ Sci Ecotechnol"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib40","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-86702-8","article-title":"Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II)","volume":"15","author":"Wahid","year":"2025","journal-title":"Sci Rep"},{"issue":"4","key":"10.1016\/j.bioflm.2026.100354_bib41","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00700-19","article-title":"Interdependency of respiratory metabolism and phenazine-associated physiology in Pseudomonas aeruginosa PA14","volume":"202","author":"Jo","year":"2020","journal-title":"J Bacteriol"},{"issue":"1","key":"10.1016\/j.bioflm.2026.100354_bib42","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.tim.2014.09.004","article-title":"Bridging the gap between viable but non-culturable and antibiotic persistent bacteria","volume":"23","author":"Ayrapetyan","year":"2015","journal-title":"Trends Microbiol"}],"container-title":["Biofilm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207526000110?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2590207526000110?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:11:49Z","timestamp":1781640709000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2590207526000110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":42,"alternative-id":["S2590207526000110"],"URL":"https:\/\/doi.org\/10.1016\/j.bioflm.2026.100354","relation":{},"ISSN":["2590-2075"],"issn-type":[{"value":"2590-2075","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bioelectrochemical detection of viable but non-culturable bacterial cells on screen-printed carbon electrodes","name":"articletitle","label":"Article Title"},{"value":"Biofilm","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioflm.2026.100354","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"100354"}}