{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T17:15:02Z","timestamp":1717780502303},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,1,16]],"date-time":"2012-01-16T00:00:00Z","timestamp":1326672000000},"content-version":"vor","delay-in-days":2693,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p><jats:bold>Summry\u2014<\/jats:bold> DNA interstrand cross\u2010links (ICL)\u2010inducing agents such as cisplatin, mitomycin C (MMC) and nitrogen mustards are widely used as potent antitumor drugs. Although ICL repair mechanism is not yet well characterized in mammalian cells, this pathway is thought to involve a sequential action of nucleotide excision repair (NER) and homologous recombination (HR). The importance of unraveling ICL repair pathways is highlighted by the hypersensitivity to ICL\u2010inducing agents in cells of patients with the genetic disease Fanconi anemia (FA) and in cells mutated in the Breast Cancer susceptibility genes BRCA1 and BRCA2. To better characterize the involvement of HR in the sensitivity to ICL\u2010inducing agents, we examined spontaneous and ICL\u2010induced HR in rodent FA\u2010like V\u2010H4 cells. In this report, we show that MMC\u2010hypersensitive V\u2010H4 cells exhibit an increased spontaneous homology\u2010directed repair (HDR) activity compared to the resistant V79 parental cells. Elevated HDR activity results mainly in increased conservative Rad51\u2010dependent recombination, without affecting non\u2010conservative single\u2010strand annealing process (SSA). We also show that HDR activity is enhanced following MMC treatment in parental cells, but not in rodent FA\u2010like V\u2010H4 cells. Moreover, our data indicate that Rad51 foci formation is significantly delayed in these FA\u2010like cells in response to crosslinking agent. These findings provide evidence for an impairment of HR control in V\u2010H4 cells and emphasize the involvement of the FA pathway in HR\u2010mediated repair.<\/jats:p>","DOI":"10.1016\/j.biolcel.2004.06.001","type":"journal-article","created":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T19:43:53Z","timestamp":1091735033000},"page":"545-552","source":"Crossref","is-referenced-by-count":8,"title":["Impairment of homologous recombination control in a Fanconi anemia\u2010like Chinese Hamster Cell mutant"],"prefix":"10.1111","volume":"96","author":[{"given":"Florence","family":"Larminat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryse","family":"Germanier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efterpi","family":"Papouli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Defais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,16]]},"container-title":["Biology of the Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.biolcel.2004.06.001","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.biolcel.2004.06.001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T23:12:59Z","timestamp":1700176379000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.biolcel.2004.06.001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1016\/j.biolcel.2004.06.001"],"URL":"https:\/\/doi.org\/10.1016\/j.biolcel.2004.06.001","archive":["Portico"],"relation":{},"ISSN":["0248-4900","1768-322X"],"issn-type":[{"value":"0248-4900","type":"print"},{"value":"1768-322X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9]]}}}