{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:47:38Z","timestamp":1789627658930,"version":"build-2803163510"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2011\/20263-0"],"award-info":[{"award-number":["2011\/20263-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biologicals"],"published-print":{"date-parts":[[2017,7]]},"DOI":"10.1016\/j.biologicals.2017.06.005","type":"journal-article","created":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T15:16:10Z","timestamp":1498576570000},"page":"6-9","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls"],"prefix":"10.1016","volume":"48","author":[{"given":"G.","family":"Nardi J\u00fanior","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Megid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.A.","family":"Mathias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Paulin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.F.","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cortez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.J.P.","family":"Listoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.H.B.","family":"Lara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.G.","family":"Motta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.G.M.","family":"Chacur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.M.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.G.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biologicals.2017.06.005_bib1","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.biologicals.2017.06.005_bib2","first-page":"296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"Godfroid","year":"2010","journal-title":"C\u00a0Med Journ"},{"key":"10.1016\/j.biologicals.2017.06.005_bib3","series-title":"Veterinary microbiology and microbial disease","first-page":"334","author":"Quinn","year":"2011"},{"key":"10.1016\/j.biologicals.2017.06.005_bib4","unstructured":"Constable PD, Hinchcliff KW, Done S, Gruenberg W. Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs, and goats. 11.ed. W.B. Saunders, Philadelphia, 2278pp."},{"key":"10.1016\/j.biologicals.2017.06.005_bib5","series-title":"Bacterial and fungal agents of animal disease","first-page":"84","author":"Songer","year":"2005"},{"key":"10.1016\/j.biologicals.2017.06.005_bib6","series-title":"Animal brucellosis","author":"Nielsen","year":"1990"},{"key":"10.1016\/j.biologicals.2017.06.005_bib7","first-page":"989","article-title":"Serological diagnosis of bovine brucellosis: a review of test performance and coast comparison","volume":"23","author":"Gall","year":"2004","journal-title":"Revue scientifique et technique. Office International des \u00c9pizooties"},{"issue":"4","key":"10.1016\/j.biologicals.2017.06.005_bib8","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1865-1682.2012.01347.x","article-title":"Detection of Brucella spp. DNA in the semen of seronegative bulls by polymerase chain reaction","volume":"60","author":"Junqueira J\u00fanior","year":"2012","journal-title":"Trans Emer Diseas"},{"key":"10.1016\/j.biologicals.2017.06.005_bib9","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.theriogenology.2005.05.032","article-title":"Welfare aspects of theriogenology: investigating alternatives to eletroejaculation of bulls","volume":"64","author":"Palmer","year":"2005","journal-title":"Theriog"},{"key":"10.1016\/j.biologicals.2017.06.005_bib10","series-title":"Techniques for the brucellosis laboratory","author":"Alton","year":"1988"},{"key":"10.1016\/j.biologicals.2017.06.005_bib11","series-title":"Manual of diagnosis test and vaccines for terrestrial animals","article-title":"Bovine brucellosis","author":"Office International Des Epizooties-OIE","year":"2010"},{"key":"10.1016\/j.biologicals.2017.06.005_bib12","first-page":"183","article-title":"Fluorescence polarization assay for the diagnosis of brucellosis: a review","volume":"22","author":"Nielsen","year":"2001","journal-title":"J\u00a0Imm Immuno"},{"key":"10.1016\/j.biologicals.2017.06.005_bib13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0022-1759(95)00121-P","article-title":"A\u00a0modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests","volume":"185","author":"Greiner","year":"1995","journal-title":"J\u00a0Im Meth"},{"key":"10.1016\/j.biologicals.2017.06.005_bib14","first-page":"107","volume":"18th","author":"Casas Olascoaga","year":"1976"},{"key":"10.1016\/j.biologicals.2017.06.005_bib15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0378-1135(02)00049-4","article-title":"Multiplex PCR for detection of Brucella spp. and Leptospira spp. DNA from aborted bovine fetuses","volume":"87","author":"Richtzenhain","year":"2002","journal-title":"Vet Microbiol"},{"issue":"10","key":"10.1016\/j.biologicals.2017.06.005_bib16","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1128\/JCM.00837-08","article-title":"Evaluation of a Multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains","volume":"46","author":"L\u00f3pez-Go\u00f1i","year":"2008","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.biologicals.2017.06.005_bib17","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.vetmic.2011.06.035","article-title":"New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis","volume":"154","author":"L\u00f3pez-Go\u00f1i","year":"2011","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.biologicals.2017.06.005_bib18","first-page":"177","article-title":"Brucella infection in bulls and the spread of brucellosis in cattle by artificial insemination. I. Intrauterine injection","volume":"87","author":"Manthei","year":"1950","journal-title":"Proc Am Vet Med Ass"},{"issue":"1","key":"10.1016\/j.biologicals.2017.06.005_bib19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1751-0813.1976.tb05363.x","article-title":"Brucella abortus infection in the bull","volume":"52","author":"Plant","year":"1976","journal-title":"Aust Vet Journ"},{"issue":"103","key":"10.1016\/j.biologicals.2017.06.005_bib20","first-page":"1152","article-title":"Studies on Brucella abortus infection in bulls","volume":"24","author":"Lambert","year":"1963","journal-title":"Am Journ Vet Res"},{"key":"10.1016\/j.biologicals.2017.06.005_bib21","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cvfa.2009.10.006","article-title":"Bovine brucellosis","volume":"26","author":"Olsen","year":"2010","journal-title":"Veterinary Clin N. Am Food Animal Pract"},{"key":"10.1016\/j.biologicals.2017.06.005_bib22","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0378-1135(02)00245-6","article-title":"Brucellosis in Brazil","volume":"90","author":"Poester","year":"2002","journal-title":"Vet Microb"},{"key":"10.1016\/j.biologicals.2017.06.005_bib23","series-title":"Brasil, Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento","year":"2009"},{"issue":"3","key":"10.1016\/j.biologicals.2017.06.005_bib24","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S0100-736X2006000300009","article-title":"Diagn\u00f3stico sorol\u00f3gico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus","volume":"26","author":"Jardim","year":"2006","journal-title":"Pesq Vet Bras"},{"key":"10.1016\/j.biologicals.2017.06.005_bib25","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1590\/S1517-83822012000200022","article-title":"Excretion of Brucella abortus vaccine B19 strain during a reprodutive cycle in dairy cows","volume":"43","author":"Pacheco","year":"2012","journal-title":"Braz J Microbiol"}],"container-title":["Biologicals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105617300714?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105617300714?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T06:28:47Z","timestamp":1657261727000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1045105617300714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":25,"alternative-id":["S1045105617300714"],"URL":"https:\/\/doi.org\/10.1016\/j.biologicals.2017.06.005","relation":{},"ISSN":["1045-1056"],"issn-type":[{"value":"1045-1056","type":"print"}],"subject":[],"published":{"date-parts":[[2017,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls","name":"articletitle","label":"Article Title"},{"value":"Biologicals","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biologicals.2017.06.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}