{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T21:37:19Z","timestamp":1763415439112},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Special Fund for Agro-scientific Research in the Public Interest of China","award":["201303046"],"award-info":[{"award-number":["201303046"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"crossref","award":["2017YFD0500706"],"award-info":[{"award-number":["2017YFD0500706"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20151360"],"award-info":[{"award-number":["BK20151360"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biologicals"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.biologicals.2018.04.002","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T13:46:13Z","timestamp":1525787173000},"page":"50-57","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Immortalization of chicken embryonic liver-derived cell line by stable expression of hMRP18S-2 for serotype 4 fowl adenovirus propagation"],"prefix":"10.1016","volume":"54","author":[{"given":"Lei","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwen","family":"Yun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixiang","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinghua","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peipei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jibo","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.biologicals.2018.04.002_bib1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/s12985-016-0644-x","article-title":"Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China","volume":"13","author":"Zhang","year":"2016","journal-title":"Virol J"},{"key":"10.1016\/j.biologicals.2018.04.002_bib2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1007\/s13337-013-0183-7","article-title":"Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens","volume":"25","author":"Mittal","year":"2014","journal-title":"Virusdisease"},{"key":"10.1016\/j.biologicals.2018.04.002_bib3","doi-asserted-by":"crossref","first-page":"e50","DOI":"10.1038\/emi.2016.50","article-title":"Outbreaks of serotype 4 fowl adenovirus with novel genotype, China","volume":"5","author":"Ye","year":"2016","journal-title":"Emerg Microb Infect"},{"key":"10.1016\/j.biologicals.2018.04.002_bib4","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/03079457.2015.1125443","article-title":"Clinicopathological characterization and genomic sequence differences observed in a highly virulent fowl Aviadenovirus serotype 4","volume":"45","author":"Vera-Hernandez","year":"2016","journal-title":"Avian Pathol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/03079459808419323","article-title":"Growth analysis of adenoviruses isolated from pigeons in chicken cells and serological characterization of the isolates","volume":"27","author":"Hess","year":"1998","journal-title":"Avian Pathol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib6","doi-asserted-by":"crossref","first-page":"347","DOI":"10.2307\/1590825","article-title":"Chicken embryo propagation of type I avian adenoviruses","volume":"32","author":"Cowen","year":"1988","journal-title":"Avian Dis"},{"key":"10.1016\/j.biologicals.2018.04.002_bib7","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jcpa.2011.05.001","article-title":"Pathogenicity of fowl adenovirus in specific pathogen free chicken embryos","volume":"146","author":"Alemnesh","year":"2012","journal-title":"J Comp Pathol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib8","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.jhep.2014.05.046","article-title":"Strategies for immortalization of primary hepatocytes","volume":"61","author":"Ramboer","year":"2014","journal-title":"J Hepatol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib9","doi-asserted-by":"crossref","first-page":"21016","DOI":"10.18632\/oncotarget.4123","article-title":"Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts","volume":"6","author":"Darekar","year":"2015","journal-title":"Oncotarget"},{"key":"10.1016\/j.biologicals.2018.04.002_bib10","doi-asserted-by":"crossref","first-page":"19363","DOI":"10.1074\/jbc.M100727200","article-title":"The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present","volume":"276","author":"Cavdar Koc","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biologicals.2018.04.002_bib11","doi-asserted-by":"crossref","first-page":"19866","DOI":"10.1073\/pnas.0911545106","article-title":"MRPS18-2 protein immortalizes primary rat embryonic fibroblasts and endows them with stem cell-like properties","volume":"106","author":"Kashuba","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.biologicals.2018.04.002_bib12","doi-asserted-by":"crossref","first-page":"5489","DOI":"10.1073\/pnas.0801053105","article-title":"EBV-encoded EBNA-6 binds and targets MRS18-2 to the nucleus, resulting in the disruption of pRb-E2F1 complexes","volume":"105","author":"Kashuba","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.biologicals.2018.04.002_bib13","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1042\/BA20070081","article-title":"Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in HEK-293 cells using polyethyleneimine-DNA complexes in combination with hypothermic treatment","volume":"50","author":"Feng","year":"2008","journal-title":"Biotechnol Appl Biochem"},{"key":"10.1016\/j.biologicals.2018.04.002_bib14","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1002\/elsc.201600110","article-title":"H9 subtype influenza vaccine in MDCK single-cell suspension culture with stable expression of TMPRSS2: generation and efficacy evaluation","volume":"16","author":"Feng","year":"2016","journal-title":"Eng Life Sci"},{"key":"10.1016\/j.biologicals.2018.04.002_bib15","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat Protoc"},{"key":"10.1016\/j.biologicals.2018.04.002_bib16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.biologicals.2018.04.002_bib17","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am J Hyg"},{"key":"10.1016\/j.biologicals.2018.04.002_bib18","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1006\/viro.1998.9290","article-title":"The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses","volume":"248","author":"Himly","year":"1998","journal-title":"Virology"},{"key":"10.1016\/j.biologicals.2018.04.002_bib19","first-page":"185","article-title":"SV40-mediated immortalization","volume":"165","author":"Tevethia","year":"2001","journal-title":"Meth Mol Biol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib20","doi-asserted-by":"crossref","first-page":"43634","DOI":"10.1074\/jbc.M402388200","article-title":"Inactivation of p16INK4a, with retention of pRB and p53\/p21cip1 function, in human MRC5 fibroblasts that overcome a telomere-independent crisis during immortalization","volume":"279","author":"Taylor","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.biologicals.2018.04.002_bib21","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1111\/j.1365-2184.2007.00462.x","article-title":"Human ovarian surface epithelial cells immortalized with hTERT maintain functional pRb and p53 expression","volume":"40","author":"Li","year":"2007","journal-title":"Cell Prolif"},{"key":"10.1016\/j.biologicals.2018.04.002_bib22","first-page":"RA12","article-title":"E2F1: a potential negative regulator of hTERT transcription in normal cells upon activation of oncogenic c-Myc","volume":"18","author":"Zhang","year":"2012","journal-title":"Med Sci Mon Int Med J Exp Clin Res : Int. Med. J. Exp. Clin. Res"},{"key":"10.1016\/j.biologicals.2018.04.002_bib23","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s10616-010-9276-y","article-title":"Regulatory mechanisms of human and mouse telomerase reverse transcriptase gene transcription: distinct dependency on c-Myc","volume":"62","author":"Fujiki","year":"2010","journal-title":"Cytotechnology"},{"key":"10.1016\/j.biologicals.2018.04.002_bib24","doi-asserted-by":"crossref","first-page":"55649","DOI":"10.18632\/oncotarget.10957","article-title":"S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma","volume":"7","author":"Mushtaq","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.biologicals.2018.04.002_bib25","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0092-8674(91)90181-W","article-title":"The retinoblastoma gene product regulates progression through the G1 phase of the cell cycle","volume":"67","author":"Goodrich","year":"1991","journal-title":"Cell"},{"key":"10.1016\/j.biologicals.2018.04.002_bib26","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1471-2121-7-27","article-title":"Contributions of differential p53 expression in the spontaneous immortalization of a chicken embryo fibroblast cell line","volume":"7","author":"Christman","year":"2006","journal-title":"BMC Cell Biol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib27","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1006\/excr.2001.5420","article-title":"Expression profiles of p53-, p16(INK4a)-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells","volume":"272","author":"Kim","year":"2002","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.biologicals.2018.04.002_bib28","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/0092-8674(92)90562-Q","article-title":"Regulation of the specific DNA binding function of p53","volume":"71","author":"Hupp","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.biologicals.2018.04.002_bib29","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/358015a0","article-title":"Cancer. p53, guardian of the genome","volume":"358","author":"Lane","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.biologicals.2018.04.002_bib30","doi-asserted-by":"crossref","first-page":"3306","DOI":"10.1038\/sj.onc.1204423","article-title":"Post-transcriptional inactivation of p53 in immortalized murine embryo fibroblast cells","volume":"20","author":"Kim","year":"2001","journal-title":"Oncogene"},{"key":"10.1016\/j.biologicals.2018.04.002_bib31","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1038\/sj.onc.1204378","article-title":"Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts","volume":"20","author":"Kim","year":"2001","journal-title":"Oncogene"},{"key":"10.1016\/j.biologicals.2018.04.002_bib32","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1158\/1541-7786.MCR-09-0317","article-title":"p57KIP2: \"Kip\"ing the cell under control","volume":"7","author":"Pateras","year":"2009","journal-title":"Mol Canc Res"},{"key":"10.1016\/j.biologicals.2018.04.002_bib33","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1515\/hsz-2011-0254","article-title":"Disorder-function relationships for the cell cycle regulatory proteins p21 and p27","volume":"393","author":"Mitrea","year":"2012","journal-title":"Biol Chem"},{"key":"10.1016\/j.biologicals.2018.04.002_bib34","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1158\/0008-5472.CAN-16-2055","article-title":"p21(WAF1) mediates cell-cycle inhibition, relevant to cancer suppression and therapy","volume":"76","author":"El-Deiry","year":"2016","journal-title":"Canc Res"},{"key":"10.1016\/j.biologicals.2018.04.002_bib35","doi-asserted-by":"crossref","first-page":"3960","DOI":"10.1016\/j.jmb.2016.08.017","article-title":"Structural conservation and E2F binding specificity within the retinoblastoma pocket protein family","volume":"428","author":"Liban","year":"2016","journal-title":"J Mol Biol"},{"key":"10.1016\/j.biologicals.2018.04.002_bib36","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1073\/pnas.0135557100","article-title":"Absence of p16INK4a and truncation of ARF tumor suppressors in chickens","volume":"100","author":"Kim","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.biologicals.2018.04.002_bib37","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/25867","article-title":"p14ARF links the tumour suppressors RB and p53","volume":"395","author":"Bates","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.biologicals.2018.04.002_bib38","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1242\/jcs.02989","article-title":"Upregulation of chicken p15INK4b at senescence and in the developing brain","volume":"119","author":"Kim","year":"2006","journal-title":"J Cell Sci"},{"key":"10.1016\/j.biologicals.2018.04.002_bib39","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1073\/pnas.0511186103","article-title":"Activated Src abrogates the Myc requirement for the G0\/G1 transition but not for the G1\/S transition","volume":"103","author":"Prathapam","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.biologicals.2018.04.002_bib40","first-page":"1039","article-title":"Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination","volume":"8","author":"Mateyak","year":"1997","journal-title":"Cell Growth Differ"},{"key":"10.1016\/j.biologicals.2018.04.002_bib41","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/s13337-017-0372-x","article-title":"Cell-culture derived fowl adenovirus serotype 4 inactivated vaccine provides complete protection for virus infection on SPF chickens","volume":"28","author":"Du","year":"2017","journal-title":"Virusdisease"}],"container-title":["Biologicals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105618300964?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105618300964?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T12:30:56Z","timestamp":1589891456000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1045105618300964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":41,"alternative-id":["S1045105618300964"],"URL":"https:\/\/doi.org\/10.1016\/j.biologicals.2018.04.002","relation":{},"ISSN":["1045-1056"],"issn-type":[{"value":"1045-1056","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immortalization of chicken embryonic liver-derived cell line by stable expression of hMRP18S-2 for serotype 4 fowl adenovirus propagation","name":"articletitle","label":"Article Title"},{"value":"Biologicals","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biologicals.2018.04.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}