{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T03:03:47Z","timestamp":1771556627694,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Instituto Pasteur\/S\u00e3o Paulo\/Brazil"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biologicals"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.biologicals.2020.08.004","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T05:48:32Z","timestamp":1598420912000},"page":"74-78","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Development of biotinylated polyclonal anti-ribonucleoprotein IgG for detection of rabies virus antigen by direct rapid immunohistochemical test"],"prefix":"10.1016","volume":"68","author":[{"given":"Bruno Stuart","family":"Castro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Guedes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Raniero","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Koike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iana Suly Santos","family":"Katz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Botelho","family":"Chaves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandriana Ramos","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.biologicals.2020.08.004_bib1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0165-0270(00)00296-X","article-title":"Rabies as a transneuronal tracer of circuits in the central nervous system","volume":"103","author":"Kelly","year":"2000","journal-title":"J Neurosci Methods"},{"key":"10.1016\/j.biologicals.2020.08.004_bib2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/B978-0-12-387040-7.00003-2","article-title":"Evasive strategies in rabies virus infection","volume":"79","author":"Lafon","year":"2011","journal-title":"Adv Virus Res"},{"key":"10.1016\/j.biologicals.2020.08.004_bib3","series-title":"Laboratory techniques in rabies","volume":"Vol. 2","year":"2018"},{"key":"10.1016\/j.biologicals.2020.08.004_bib4","series-title":"Rabies","author":"OIE-World Organisation for Animal Health","year":"2020"},{"issue":"3","key":"10.1016\/j.biologicals.2020.08.004_bib5","first-page":"1","article-title":"Additional progress in the development and application of a direct, rapid immunohistochemical test for rabies diagnosis","volume":"5","author":"Rupprecht","year":"2018","journal-title":"Veterinary Sciences"},{"key":"10.1016\/j.biologicals.2020.08.004_bib6","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ijid.2016.03.016","article-title":"Laboratory diagnostics in dog-mediated rabies: an overview of performance and a proposed strategy for various settings","volume":"46","author":"Duong","year":"2016","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.biologicals.2020.08.004_bib7","article-title":"Rabies diagnosis for developing countries","volume":"2","author":"Durr","year":"2008","journal-title":"PLoS NTD"},{"key":"10.1016\/j.biologicals.2020.08.004_bib8","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3201\/eid1202.050812","article-title":"Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis","volume":"12","author":"Lembo","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.biologicals.2020.08.004_bib9","article-title":"Enhanced rabies surveillance using a direct rapid immunohistochemical test","volume":"146","author":"Rupprecht","year":"2019","journal-title":"J Vis Exp. v."},{"issue":"9","key":"10.1016\/j.biologicals.2020.08.004_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0003189","article-title":"Comparison of biotinylated monoclonal and polyclonal antibodies in an evaluation of a direct rapid immunohistochemical test for the routine diagnosis of rabies in southern africa","volume":"8","author":"Coetzer","year":"2014","journal-title":"PLoS Neglected Trop Dis"},{"key":"10.1016\/j.biologicals.2020.08.004_bib11","series-title":"Validation and operational application of a rapid method for rabies antigen detection","first-page":"42","author":"Saturday","year":"2009"},{"issue":"5","key":"10.1016\/j.biologicals.2020.08.004_bib12","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s12250-012-3265-6","article-title":"Evaluation of a direct rapid immunohistochemical test (dRIT) for rapid diagnosis of rabies in animals and humans","volume":"27","author":"Madhusudana","year":"2012","journal-title":"Virol Sin"},{"key":"10.1016\/j.biologicals.2020.08.004_bib13","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jviromet.2018.02.019","article-title":"Evaluation of monoclonal antibody-based direct, rapid immunohistochemical test for rabies diagnosis","volume":"256","author":"Feng","year":"2018","journal-title":"J Virol Methods"},{"issue":"5","key":"10.1016\/j.biologicals.2020.08.004_bib14","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1503\/cmaj.071488","article-title":"Rabies in India","volume":"178","author":"Menezes","year":"2008","journal-title":"Can Med Assoc J"},{"key":"10.1016\/j.biologicals.2020.08.004_bib15","first-page":"1","article-title":"Laboratory diagnosis of human rabies: recent advances","author":"Madhusudana","year":"2013","journal-title":"Sci World J"},{"key":"10.1016\/j.biologicals.2020.08.004_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2019.03.007","article-title":"Purification of IgG against ribonucleoprotein by a homemade immunoaffinity chromatography column for rabies diagnosis","volume":"471","author":"da Silva Santos","year":"2019","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.biologicals.2020.08.004_bib17","first-page":"384","article-title":"Spectrophotometric method for the determination of protein and nucleic acids","volume":"310","author":"Warburg","year":"1941","journal-title":"Biochem Z"},{"issue":"1","key":"10.1016\/j.biologicals.2020.08.004_bib18","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0022-1759(86)90268-1","article-title":"Antibody avidity determination by ELISA using thiocyanate elution","volume":"86","author":"Pullen","year":"1986","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.biologicals.2020.08.004_bib19","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.3389\/fimmu.2017.01175","article-title":"Adjuvant-Mediated epitope specificity and enhanced neutralizing activity of antibodies targeting dengue virus envelope protein","volume":"8","author":"Maeda","year":"2017","journal-title":"Front Immunol"},{"key":"10.1016\/j.biologicals.2020.08.004_bib20","article-title":"Enhanced rabies surveillance using a direct rapid immunohistochemical test","volume":"146","author":"Patrick","year":"2019","journal-title":"JoVE"},{"key":"10.1016\/j.biologicals.2020.08.004_bib21","first-page":"73","article-title":"The fluorescent antibody test","volume":"vol. 23","author":"Dean","year":"1996"},{"issue":"31","key":"10.1016\/j.biologicals.2020.08.004_bib22","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1016\/S0264-410X(01)00186-4","article-title":"Rapid clearance of SAG-2 rabies virus from dogs after oral vaccination","volume":"19","author":"Orciari","year":"2001","journal-title":"Vaccine"},{"issue":"12","key":"10.1016\/j.biologicals.2020.08.004_bib23","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1038\/ki.2009.92","article-title":"Diagnostic methods I: sensitivity, specificity, and other measures of accuracy","volume":"75","author":"Van Stralen","year":"2009","journal-title":"Kidney Int"},{"key":"10.1016\/j.biologicals.2020.08.004_bib24","series-title":"Terrestrial manual 8th edition, rabies. Infection with rabies virus and otherLyssaviruses","author":"OIE","year":"2018"},{"key":"10.1016\/j.biologicals.2020.08.004_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2020.105340","article-title":"Evaluation of polyclonal anti-RNP IgG antibody for rabies diagnosis by indirect rapid immunohistochemistry test","volume":"206","author":"Bartaquini","year":"2020","journal-title":"Acta Trop"},{"key":"10.1016\/j.biologicals.2020.08.004_bib26","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/S0076-6879(83)93050-1","article-title":"Preparation of antibody-linked cytotoxic agents","volume":"93","author":"Ghose","year":"1983","journal-title":"Methods Enzymol"},{"issue":"1","key":"10.1016\/j.biologicals.2020.08.004_bib27","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1006\/viro.1998.9230","article-title":"Herpes simplex virus type 1 VP26 is not essential for replication in cell culture but influences production of infectious virus in the nervous system of infected mice","volume":"247","author":"Person","year":"1998","journal-title":"Virology"},{"issue":"48","key":"10.1016\/j.biologicals.2020.08.004_bib28","doi-asserted-by":"crossref","first-page":"14927","DOI":"10.1021\/bi0018786","article-title":"Intrinsic fluorescence of the P-glycoprotein multidrug transporter: sensitivity of tryptophan residues to binding of drugs and nucleotides","volume":"39","author":"Sharom","year":"2000","journal-title":"Biochemistry"},{"issue":"1","key":"10.1016\/j.biologicals.2020.08.004_bib29","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1369\/jhc.2010.956920","article-title":"Controls for immunocytochemistry: an update","volume":"59","author":"Burry","year":"2011","journal-title":"J Histochem Cytochem"},{"issue":"3","key":"10.1016\/j.biologicals.2020.08.004_bib30","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1210\/en.2013-1971","article-title":"Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them","volume":"155","author":"Ivell","year":"2014","journal-title":"Endocrinology"},{"issue":"3","key":"10.1016\/j.biologicals.2020.08.004_bib31","first-page":"127","volume":"65","author":"Ascoli","year":"2018","journal-title":"Overlooked benefits of using polyclonal antibodies Biotechniques"},{"issue":"9","key":"10.1016\/j.biologicals.2020.08.004_bib32","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1007\/s00705-018-3829-3","article-title":"Analysis of rabies diagnosis in dogs and cats in the state of S\u00e3o Paulo, Brazil","volume":"163","author":"Castilho","year":"2018","journal-title":"Arch Virol"},{"issue":"1","key":"10.1016\/j.biologicals.2020.08.004_bib33","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00705-016-3079-1","article-title":"Phylogenetic analysis of rabies virus isolated from canids in north and northeast Brazil","volume":"162","author":"Nunes de Souza","year":"2017","journal-title":"Arch Virol"}],"container-title":["Biologicals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105620300944?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105620300944?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:14:58Z","timestamp":1760922898000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1045105620300944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":33,"alternative-id":["S1045105620300944"],"URL":"https:\/\/doi.org\/10.1016\/j.biologicals.2020.08.004","relation":{},"ISSN":["1045-1056"],"issn-type":[{"value":"1045-1056","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of biotinylated polyclonal anti-ribonucleoprotein IgG for detection of rabies virus antigen by direct rapid immunohistochemical test","name":"articletitle","label":"Article Title"},{"value":"Biologicals","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biologicals.2020.08.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}