{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:43:55Z","timestamp":1792719835421,"version":"4.5.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC 101-2628-E-007-011-MY3"],"award-info":[{"award-number":["NSC 101-2628-E-007-011-MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["102-2221-E-007-031"],"award-info":[{"award-number":["102-2221-E-007-031"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomaterials"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.biomaterials.2014.01.030","type":"journal-article","created":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T21:15:21Z","timestamp":1390943721000},"page":"3729-3735","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":77,"title":["Monitoring the VEGF level in aqueous humor of patients with ophthalmologically relevant diseases via ultrahigh sensitive paper-based ELISA"],"prefix":"10.1016","volume":"35","author":[{"given":"Min-Yen","family":"Hsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Yao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hsin","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng-Hung","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Cheng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siu-Fung","family":"Chau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hin-Yeung","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Min","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biomaterials.2014.01.030_bib1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0002-9394(14)75794-0","article-title":"Increased vascular endothelial growth factor levels in the vitreous of eyes with proliferative diabetic retinopathy","volume":"118","author":"Adamis","year":"1994","journal-title":"Am J Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib2","first-page":"335","article-title":"Role of vascular endothelial growth factor in ocular angiogenesis","volume":"19","author":"Shams","year":"2006","journal-title":"Ophthalmol Clin North Am"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib3","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1056\/NEJMcp1003934","article-title":"Clinical practice. Retinal-vein occlusion","volume":"363","author":"Wong","year":"2010","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib4","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1056\/NEJMoa054481","article-title":"Ranibizumab for neovascular age-related macular degeneration","volume":"355","author":"Rosenfeld","year":"2006","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib5","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1001\/archopht.125.10.1363","article-title":"Vascular endothelial growth factor in aqueous humor before and after intravitreal injection of bevacizumab in eyes with diabetic retinopathy","volume":"125","author":"Sawada","year":"2007","journal-title":"Arch Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib6","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1056\/NEJMra062326","article-title":"Age-related macular degeneration","volume":"355","author":"de Jong","year":"2006","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib7","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00417-004-0950-7","article-title":"Aqueous humor levels of cytokines are related to vitreous levels and progression of diabetic retinopathy in diabetic patients","volume":"243","author":"Funatsu","year":"2005","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib8","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1016\/j.ophtha.2009.04.038","article-title":"Monitoring ocular drug therapy by analysis of aqueous samples","volume":"116","author":"Campochiaro","year":"2009","journal-title":"Ophthalmology"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib9","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s11064-010-0328-7","article-title":"Validation of molecular and genomic biomarkers of retinal drug efficacy: use of ocular fluid sampling to evaluate VEGF","volume":"36","author":"Sharma","year":"2011","journal-title":"Neurochem Res"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib10","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S0002-9394(01)01269-7","article-title":"Increased levels of vascular endothelial growth factor and interleukin-6 in the aqueous humor of diabetics with macular edema","volume":"133","author":"Funatsu","year":"2002","journal-title":"Am J Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1097\/00006982-200601000-00002","article-title":"Pars plana vitrectomy with and without peeling of the inner limiting membrane for diabetic macular edema","volume":"26","author":"Patel","year":"2006","journal-title":"Retina"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib12","first-page":"60","article-title":"Multiplex immunoassay analysis of biomarkers in clinically accessible quantities of human aqueous humor","volume":"15","author":"Sharma","year":"2009","journal-title":"Mol Vis"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib13","doi-asserted-by":"crossref","first-page":"4771","DOI":"10.1002\/anie.201001005","article-title":"P-ELISA","volume":"49","author":"Cheng","year":"2010","journal-title":"Angew Chem Int Ed"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib14","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1136\/bjo.2009.159160","article-title":"Ranibizumab (Lucentis) in neovascular age-related macular degeneration: evidence from clinical trials","volume":"94","author":"Mitchell","year":"2010","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib15","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1039\/c3lc50135c","article-title":"Molecular-level dengue fever diagnostic devices made out of paper","volume":"13","author":"Lo","year":"2013","journal-title":"Lab Chip"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib16","doi-asserted-by":"crossref","unstructured":"Wang H-K, Tsai C-C, Chen K-H, Tang C-T, Leou J-S, Li P-C, et\u00a0al. Cellulose-based diagnostic devices for diagnosing serotype-2 dengue fever in human serum. Adv Healthc Mater. http:\/\/dx.doi.org\/10.1002\/adhm.201300150.","DOI":"10.1002\/adhm.201300150"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib17","doi-asserted-by":"crossref","first-page":"3699","DOI":"10.1021\/ac800112r","article-title":"Simple telemedicine for developing regions camera phones and paper based microfluidic devices for real time, off site diagnosis","volume":"80","author":"Martinez","year":"2008","journal-title":"Anal Chem"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib18","doi-asserted-by":"crossref","first-page":"152ra129","DOI":"10.1126\/scitranslmed.3003981","article-title":"A\u00a0paper-based multiplexed transaminase test for low-cost, point-of-care liver function testing","volume":"4","author":"Pollock","year":"2012","journal-title":"Sci Trans Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib19","doi-asserted-by":"crossref","unstructured":"Wu H-Y, Rubinstein M, Shih E, Guttag J, Durand F, Freeman W. Eulerian video magnification for revealing subtle changes in the world. ACM T Graph;31:65.","DOI":"10.1145\/2185520.2335416"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib20","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1136\/bjo.77.2.107","article-title":"Detection of Toxoplasma gondii in aqueous humour by the polymerase chain reaction","volume":"77","author":"Aouizerate","year":"1993","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib21","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.1128\/JCM.37.11.3465-3468.1999","article-title":"Value of PCR for detection of Toxoplasma gondii in aqueous humor and blood samples from immunocompetent patients with ocular toxoplasmosis","volume":"37","author":"Bou","year":"1999","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib22","doi-asserted-by":"crossref","first-page":"973","DOI":"10.2174\/156652409789712783","article-title":"Current molecular understanding and future treatment strategies for pathologic ocular neovascularization","volume":"9","author":"Tolentino","year":"2009","journal-title":"Curr Mol Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib23","first-page":"434","article-title":"Pharmacokinetic rationale for dosing every 2 weeks versus 4 weeks with intravitreal ranibizumab, bevacizumab, and aflibercept (vascular endothelial growth factor trap-eye)","volume":"32","author":"Stewart","year":"2012","journal-title":"Retina"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib24","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.ajo.2008.05.036","article-title":"Intraocular pharmacokinetics of bevacizumab after a single intravitreal injection in humans","volume":"146","author":"Krohne","year":"2008","journal-title":"Am J Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib25","first-page":"81","article-title":"Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples","volume":"5","author":"Balaiya","year":"2011","journal-title":"Clin Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib26","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1056\/NEJMct1000495","article-title":"Ranibizumab therapy for neovascular age-related macular degeneration","volume":"363","author":"Folk","year":"2010","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib27","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1185\/03007995.2011.585394","article-title":"A\u00a0systematic review of the efficacy and safety outcomes of anti-VEGF agents used for treating neovascular age-related macular degeneration: comparison of ranibizumab and bevacizumab","volume":"27","author":"Mitchell","year":"2011","journal-title":"Curr Med Res Opin"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib28","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1097\/ICU.0b013e32835f8bbe","article-title":"Systemic effects of intravitreal vascular endothelial growth factor inhibitors","volume":"24","author":"Campbell","year":"2013","journal-title":"Curr Opin Ophthalmol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1046\/j.1365-2265.1997.2331047.x","article-title":"Clinical activity score as a guide in the management of patients with Graves' ophthalmopathy","volume":"47","author":"Mourits","year":"1997","journal-title":"Clin Endocrinol"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib30","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1097\/01.ico.0000240086.56522.69","article-title":"Treatment of epithelial ingrowth after LASIK enhancement with a combined technique of mechanical debridement, flap suturing, and fibrin glue application","volume":"25","author":"Narv\u00e1ez","year":"2006","journal-title":"Cornea"},{"key":"10.1016\/j.biomaterials.2014.01.030_bib31","doi-asserted-by":"crossref","first-page":"e36451","DOI":"10.1371\/journal.pone.0036451","article-title":"Human tears reveal insights into corneal neovascularization","volume":"7","author":"Zakaria","year":"2012","journal-title":"PLoS One"}],"container-title":["Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961214000325?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961214000325?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T15:56:40Z","timestamp":1758902200000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0142961214000325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S0142961214000325"],"URL":"https:\/\/doi.org\/10.1016\/j.biomaterials.2014.01.030","relation":{},"ISSN":["0142-9612"],"issn-type":[{"value":"0142-9612","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Monitoring the VEGF level in aqueous humor of patients with ophthalmologically relevant diseases via ultrahigh sensitive paper-based ELISA","name":"articletitle","label":"Article Title"},{"value":"Biomaterials","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biomaterials.2014.01.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}