{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:46:08Z","timestamp":1786985168997,"version":"build-2736575974"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH DE 013380"],"award-info":[{"award-number":["NIH DE 013380"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE 015411"],"award-info":[{"award-number":["DE 015411"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomaterials"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1016\/j.biomaterials.2015.09.021","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T04:26:30Z","timestamp":1442291190000},"page":"120-130","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide"],"prefix":"10.1016","volume":"73","author":[{"given":"Janani","family":"Ramaswamy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7356-4905","authenticated-orcid":false,"given":"Hwa Kyung","family":"Nam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsha","family":"Ramaraju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan E.","family":"Hatch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Kohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biomaterials.2015.09.021_bib1","doi-asserted-by":"crossref","first-page":"4385","DOI":"10.1016\/S0142-9612(03)00343-0","article-title":"RGD modified polymers: biomaterials for stimulated cell adhesion and beyond","volume":"24","author":"Hersel","year":"2003","journal-title":"Biomaterials"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0167-4838(95)00190-5","article-title":"Acidic amino acid-rich sequences as binding sites of osteonectin to hydroxyapatite crystals","volume":"1292","author":"Fujisawa","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0945-053X(97)90113-X","article-title":"Attachment of osteoblastic cells to hydroxyapatite by a synthetic peptide (Glu7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein","volume":"16","author":"Fujisawa","year":"1997","journal-title":"Matrix Biol."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib4","doi-asserted-by":"crossref","first-page":"16213","DOI":"10.1074\/jbc.M001773200","article-title":"Chimeric peptides of statherin and osteopontin that bind hydroxyapatite and mediate cell adhesion","volume":"275","author":"Gilbert","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.actbio.2009.08.003","article-title":"Modular peptides promote human mesenchymal stem cell differentiation on biomaterial surfaces","volume":"6","author":"Lee","year":"2010","journal-title":"Acta Biomater."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib6","doi-asserted-by":"crossref","first-page":"e50378","DOI":"10.1371\/journal.pone.0050378","article-title":"Coating with a modular bone morphogenetic peptide promotes healing of a bone-implant gap in an ovine model","volume":"7","author":"Lu","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1159\/000151380","article-title":"Identification of peptides with targeted adhesion to bone-like mineral via phage display and computational modeling","volume":"189","author":"Segvich","year":"2008","journal-title":"Cells Tissues Organs"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib8","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1016\/j.biomaterials.2008.11.008","article-title":"The adsorption of preferential binding peptides to apatite-based materials","volume":"30","author":"Segvich","year":"2009","journal-title":"Biomaterials"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib9","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1749-6632.1995.tb44642.x","article-title":"The Inhibitory activity of osteopontin on hydroxyapatite formation in\u00a0vitro","volume":"760","author":"Goldberg","year":"1995","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib10","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1359\/jbmr.090832","article-title":"Phosphorylation-dependent inhibition of mineralization by osteopontin ASARM peptides is regulated by PHEX cleavage","volume":"25","author":"Addison","year":"2009","journal-title":"J.\u00a0Bone Min. Res."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib11","doi-asserted-by":"crossref","first-page":"9422","DOI":"10.1016\/j.biomaterials.2010.08.064","article-title":"Phosphorylation-dependent mineral-type specificity for apatite-binding peptide sequences","volume":"31","author":"Addison","year":"2010","journal-title":"Biomaterials"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib12","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1002\/art.1780370624","article-title":"Causal link between nucleotide pyrophosphohydrolase overactivity and increased intracellular inorganic pyrophosphate generation demonstrated by transfection of cultured fibroblasts and osteoblasts with plasma cell membrane glycoprotein-1","volume":"37","author":"Terkeltaub","year":"1994","journal-title":"Arthritis Rheum."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1126\/science.289.5477.265","article-title":"Role of the mouse ank gene in control of tissue calcification and arthritis","volume":"289","author":"Ho","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1042\/bj1020053","article-title":"Association of inorganic-pyrophosphatase activity with human alkaline phosphatase preparations","volume":"102","author":"Moss","year":"1967","journal-title":"Biochem. J."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib15","doi-asserted-by":"crossref","first-page":"9445","DOI":"10.1073\/pnas.142063399","article-title":"Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization","volume":"99","author":"Hessle","year":"2002","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib16","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1161\/hh2401.101070","article-title":"Smooth muscle cell phenotypic transition associated with calcification: upregulation of Cbfa1 and downregulation of smooth muscle lineage markers","volume":"89","author":"Steitz","year":"2001","journal-title":"Circ. Res."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib17","doi-asserted-by":"crossref","first-page":"e32177","DOI":"10.1371\/journal.pone.0032177","article-title":"Altered bone development and an increase in FGF-23 expression in Enpp1 mice","volume":"7","author":"Mackenzie","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib18","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1161\/CIRCULATIONAHA.104.488916","article-title":"Coronary calcification improves cardiovascular risk prediction in the elderly","volume":"112","author":"Vliegenthart","year":"2005","journal-title":"Circulation"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib19","first-page":"e18","article-title":"Heart disease and stroke statistics\u20132011 update: a report from the American Heart Association","volume":"123","author":"Roger","year":"2011","journal-title":"Circulation"},{"key":"10.1016\/j.biomaterials.2015.09.021_bib20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00223-004-1288-1","article-title":"Importance of phosphorylation for osteopontin regulation of biomineralization","volume":"77","author":"Gericke","year":"2005","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib21","doi-asserted-by":"crossref","first-page":"20197","DOI":"10.1074\/jbc.M909174199","article-title":"Phosphorylation of osteopontin is required for inhibition of vascular smooth muscle cell calcification","volume":"275","author":"Jono","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib22","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0169-6009(08)80225-5","article-title":"Osteopontin-hydroxyapatite interactions in\u00a0vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel","volume":"22","author":"Boskey","year":"1993","journal-title":"Bone Min."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib23","doi-asserted-by":"crossref","first-page":"8562","DOI":"10.1073\/pnas.90.18.8562","article-title":"Nucleation of hydroxyapatite by bone sialoprotein","volume":"90","author":"Hunter","year":"1993","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib24","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1089\/ten.2004.10.1623","article-title":"Exogenous Runx2 expression enhances in\u00a0vitro osteoblastic differentiation and mineralization in primary bone marrow stromal cells","volume":"10","author":"Byers","year":"2004","journal-title":"Tissue Eng."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib25","doi-asserted-by":"crossref","first-page":"8352","DOI":"10.1073\/pnas.140021997","article-title":"Phosphate is a specific signal for induction of osteopontin gene expression","volume":"97","author":"Beck","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib26","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S0014-4827(03)00213-1","article-title":"Inorganic phosphate regulates multiple genes during osteoblast differentiation, including Nrf2","volume":"288","author":"Beck","year":"2003","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib27","doi-asserted-by":"crossref","first-page":"15872","DOI":"10.1074\/jbc.M701116200","article-title":"Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity","volume":"282","author":"Addison","year":"2007","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.biomaterials.2015.09.021_bib28","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1002\/jcb.10622","article-title":"Inorganic phosphate as a signaling molecule in osteoblast differentiation","volume":"90","author":"Beck","year":"2003","journal-title":"J.\u00a0Cell Biochem."}],"container-title":["Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961215007668?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961215007668?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T16:11:40Z","timestamp":1758903100000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0142961215007668"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":28,"alternative-id":["S0142961215007668"],"URL":"https:\/\/doi.org\/10.1016\/j.biomaterials.2015.09.021","relation":{},"ISSN":["0142-9612"],"issn-type":[{"value":"0142-9612","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide","name":"articletitle","label":"Article Title"},{"value":"Biomaterials","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biomaterials.2015.09.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}