{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:07:08Z","timestamp":1770977228921,"version":"3.50.1"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2024NSFSC0290"],"award-info":[{"award-number":["2024NSFSC0290"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2024YFA1210704"],"award-info":[{"award-number":["2024YFA1210704"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22107082"],"award-info":[{"award-number":["22107082"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61821002"],"award-info":[{"award-number":["61821002"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic Chemistry"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.bioorg.2026.109571","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T07:49:05Z","timestamp":1769586545000},"page":"109571","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Development of selective NBD-based fluorescent probes for in vitro detection of factor XIa and Thrombus imaging"],"prefix":"10.1016","volume":"171","author":[{"given":"Xinyu","family":"Zhuang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengjiao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijuan","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bioorg.2026.109571_bb0005","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1161\/ATVBAHA.124.320151","article-title":"Global Health burden of venous thromboembolism","volume":"44","author":"Wendelboe","year":"2024","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bioorg.2026.109571_bb0010","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1016\/j.jacc.2025.08.015","article-title":"Global, regional, and National Burden of cardiovascular diseases and risk factors in 204 countries and territories, 1990-2023","volume":"86","author":"D. Global Burden of Cardiovascular","year":"2025","journal-title":"J. Am. Coll. Cardiol."},{"key":"10.1016\/j.bioorg.2026.109571_bb0015","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41573-020-0061-0","article-title":"Therapeutic strategies for thrombosis: new targets and approaches","volume":"19","author":"Mackman","year":"2020","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bioorg.2026.109571_bb0020","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1182\/blood-2018-06-820746","article-title":"Treatment of bleeding complications in patients on anticoagulant therapy","volume":"133","author":"Piran","year":"2019","journal-title":"Blood"},{"key":"10.1016\/j.bioorg.2026.109571_bb0025","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1007\/s12350-017-0942-8","article-title":"An unmet clinical need: the history of thrombus imaging","volume":"26","author":"Lanza","year":"2019","journal-title":"J. Nucl. Cardiol."},{"key":"10.1016\/j.bioorg.2026.109571_bb0030","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1111\/jth.14534","article-title":"Predicting the risk of recurrent venous thrombosis: what the future might bring","volume":"17","author":"Lijfering","year":"2019","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.bioorg.2026.109571_bb0035","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1097\/ALN.0000000000003903","article-title":"Anticoagulation monitoring for perioperative physicians","volume":"135","author":"Maier","year":"2021","journal-title":"Anesthesiology"},{"key":"10.1016\/j.bioorg.2026.109571_bb0040","doi-asserted-by":"crossref","first-page":"B65","DOI":"10.1093\/eurheartjsupp\/suad070","article-title":"The new in anticoagulation: factor XI inhibitors","volume":"25","author":"Muscente","year":"2023","journal-title":"Eur. Heart J. Suppl."},{"key":"10.1016\/j.bioorg.2026.109571_bb0045","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1161\/CIRCRESAHA.118.313155","article-title":"Antithrombotic agents","volume":"124","author":"Chan","year":"2019","journal-title":"Circ. Res."},{"key":"10.1016\/j.bioorg.2026.109571_bb0050","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1016\/S0140-6736(20)32439-9","article-title":"Direct oral anticoagulants: evidence and unresolved issues","volume":"396","author":"Chan","year":"2020","journal-title":"Lancet"},{"key":"10.1016\/j.bioorg.2026.109571_bb0055","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1021\/acs.jmedchem.2c02130","article-title":"Factor XIa inhibitors in anticoagulation therapy: recent advances and perspectives","volume":"66","author":"Xie","year":"2023","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0060","doi-asserted-by":"crossref","DOI":"10.1007\/s40265-024-02049-w","article-title":"Therapeutic potential of FXI inhibitors: hype or Hope?","author":"Galli","year":"2024","journal-title":"Drugs"},{"key":"10.1016\/j.bioorg.2026.109571_bb0065","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1021\/acs.jmedchem.1c00613","article-title":"Discovery of Milvexian, a high-affinity, orally bioavailable inhibitor of factor XIa in clinical studies for antithrombotic therapy","volume":"65","author":"Dilger","year":"2022","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0070","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1182\/blood-2016-09-742262","article-title":"Factor XI deficiency is associated with lower risk for cardiovascular and venous thromboembolism events","volume":"129","author":"Preis","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.bioorg.2026.109571_bb0075","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/rth2.12467","article-title":"Detecting factor XIa in immune globulin products: commutability of international reference materials for traditional and global hemostasis assays","volume":"5","author":"Liang","year":"2021","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"10.1016\/j.bioorg.2026.109571_bb0080","first-page":"27","article-title":"Determination of procoagulant activity in human normal immunoglobulin preparations for therapeutic use by FXIa chromogenic assay: evaluation of test kit sensitivity, reference standard performance and product formulation effects on the FXIa assay","volume":"2024","author":"Behr-Gross","year":"2024","journal-title":"Pharmeur. Bio Sci. Notes"},{"key":"10.1016\/j.bioorg.2026.109571_bb0085","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1161\/CIRCULATIONAHA.122.061132","article-title":"What is the future of factor XI inhibitors?","volume":"146","author":"Weitz","year":"2022","journal-title":"Circulation"},{"key":"10.1016\/j.bioorg.2026.109571_bb0090","doi-asserted-by":"crossref","first-page":"4246","DOI":"10.1111\/bph.15635","article-title":"Molecular imaging of arterial and venous thrombosis","volume":"178","author":"Wang","year":"2021","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.bioorg.2026.109571_bb0095","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1093\/eurheartj\/ehac464","article-title":"Factor XI inhibitors: cardiovascular perspectives","volume":"44","author":"De Caterina","year":"2023","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.bioorg.2026.109571_bb0100","doi-asserted-by":"crossref","first-page":"7106","DOI":"10.1021\/acs.chemrev.3c00776","article-title":"Fluorescent probes for disease diagnosis","volume":"124","author":"Wang","year":"2024","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bioorg.2026.109571_bb0105","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1021\/acs.jmedchem.2c00845","article-title":"Fluorescent activity-based probe to image and inhibit factor XIa activity in human plasma","volume":"66","author":"Modrzycka","year":"2023","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0110","doi-asserted-by":"crossref","first-page":"21174","DOI":"10.1039\/D5SC05683G","article-title":"Chemical probes for enzyme imaging: challenges in design, synthesis and biomedical applications","volume":"16","author":"Nguyen","year":"2025","journal-title":"Chem. Sci."},{"key":"10.1016\/j.bioorg.2026.109571_bb0115","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.jcmg.2022.10.002","article-title":"Noninvasive in vivo coronary artery Thrombus imaging","volume":"16","author":"Tzolos","year":"2023","journal-title":"JACC Cardiovasc. Imaging"},{"key":"10.1016\/j.bioorg.2026.109571_bb0120","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/S0140-6736(22)00456-1","article-title":"Safety of the oral factor XIa inhibitor asundexian compared with apixaban in patients with atrial fibrillation (PACIFIC-AF): a multicentre, randomised, double-blind, double-dummy, dose-finding phase 2 study","volume":"399","author":"Piccini","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.bioorg.2026.109571_bb0125","doi-asserted-by":"crossref","DOI":"10.1021\/acs.jmedchem.3c00795","article-title":"Design and preclinical characterization program toward Asundexian (BAY 2433334), an Oral factor XIa inhibitor for the prevention and treatment of thromboembolic disorders","author":"Roehrig","year":"2023","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0130","doi-asserted-by":"crossref","first-page":"7436","DOI":"10.1039\/D0CS01096K","article-title":"NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications","volume":"50","author":"Jiang","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.bioorg.2026.109571_bb0135","doi-asserted-by":"crossref","first-page":"4785","DOI":"10.1002\/anie.201915744","article-title":"A general strategy to enhance donor-acceptor molecules using solvent-excluding substituents","volume":"59","author":"Hoelzel","year":"2020","journal-title":"Angew. Chem. Int. Ed. Eng."},{"key":"10.1016\/j.bioorg.2026.109571_bb0140","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1039\/D1MD00170A","article-title":"Polarity-based fluorescence probes: properties and applications","volume":"12","author":"Qin","year":"2021","journal-title":"RSC Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0145","doi-asserted-by":"crossref","first-page":"10109","DOI":"10.1021\/jp203532c","article-title":"Interaction of 7-nitrobenz-2-oxa-1,3-diazol-4-yl-labeled fatty amines with 1-palmitoyl, 2-oleoyl-sn-glycero-3-phosphocholine bilayers: a molecular dynamics study","volume":"115","author":"Filipe","year":"2011","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bioorg.2026.109571_bb0150","doi-asserted-by":"crossref","first-page":"5638","DOI":"10.1021\/acs.jmedchem.7b00338","article-title":"Development and characterization of a fluorescent tracer for the free fatty acid receptor 2 (FFA2\/GPR43)","volume":"60","author":"Hansen","year":"2017","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0155","doi-asserted-by":"crossref","first-page":"6183","DOI":"10.1021\/acs.jcim.3c01153","article-title":"AmberTools","volume":"63","author":"Case","year":"2023","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.bioorg.2026.109571_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2024.117067","article-title":"Discovery of potent and selective factor XIa inhibitors incorporating triazole-based benzoic acid as novel P2' fragments: molecular dynamics simulations and anticoagulant activity","volume":"282","author":"Dai","year":"2025","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0165","series-title":"Preparative process of two 4-{[(2S)-2-{4-[5-chloro-2-(1H-1,2,3-triazol-1-yl)phenyl]-5-methoxy-2-oxopyridin-1(2H)-yl}butanoyl]amino}-2-fluorobenzamide derivatives","author":"Egger","year":"2019"},{"key":"10.1016\/j.bioorg.2026.109571_bb0170","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1432-1033.1988.tb13849.x","article-title":"Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin","volume":"172","author":"Kawabata","year":"1988","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0175","doi-asserted-by":"crossref","first-page":"4817","DOI":"10.1016\/j.jmb.2019.09.008","article-title":"Allosteric inhibition as a new mode of action for BAY 1213790, a neutralizing antibody targeting the activated form of coagulation factor XI","volume":"431","author":"Schaefer","year":"2019","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bioorg.2026.109571_bb0180","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1111\/jth.15700","article-title":"Pharmacological profile of asundexian, a novel, orally bioavailable inhibitor of factor XIa","volume":"20","author":"Heitmeier","year":"2022","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.bioorg.2026.109571_bb0185","series-title":"Principles of Fluorescence Spectroscopy","first-page":"205","author":"Solvent and Environmental Effects","year":"2006"},{"key":"10.1016\/j.bioorg.2026.109571_bb0190","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1039\/C5SC01076D","article-title":"A structural remedy toward bright dipolar fluorophores in aqueous media","volume":"6","author":"Singha","year":"2015","journal-title":"Chem. Sci."},{"key":"10.1016\/j.bioorg.2026.109571_bb0195","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1021\/acs.accounts.6b00517","article-title":"Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications","volume":"50","author":"Klymchenko","year":"2017","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.bioorg.2026.109571_bb0200","doi-asserted-by":"crossref","first-page":"12656","DOI":"10.1039\/D1CS00239B","article-title":"Twisted intramolecular charge transfer (TICT) and twists beyond TICT: from mechanisms to rational designs of bright and sensitive fluorophores","volume":"50","author":"Wang","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.bioorg.2026.109571_bb0205","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1021\/ct400341p","article-title":"PTRAJ and CPPTRAJ: software for processing and analysis of molecular dynamics trajectory data","volume":"9","author":"Roe","year":"2013","journal-title":"J. Chem. Theory Comput."},{"key":"10.1016\/j.bioorg.2026.109571_bb0210","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1021\/ar200135h","article-title":"Hydrogen bonding in the electronic excited state","volume":"45","author":"Zhao","year":"2012","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.bioorg.2026.109571_bb0215","doi-asserted-by":"crossref","first-page":"5538","DOI":"10.1038\/s41467-024-49858-x","article-title":"Accurate prediction of protein structural flexibility by deep learning integrating intricate atomic structures and Cryo-EM density information","volume":"15","author":"Song","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bioorg.2026.109571_bb0220","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra precision glide: docking and scoring incorporating a model of hydrophobic enclosure for protein-ligand complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0225","first-page":"12","article-title":"Protoss: a holistic approach to predict tautomers and protonation states in protein-ligand complexes","volume":"6","author":"Bietz","year":"2014","journal-title":"J. Chemother."},{"key":"10.1016\/j.bioorg.2026.109571_bb0230","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1002\/jcc.10128","article-title":"Fast, efficient generation of high-quality atomic charges. AM1-BCC model: II. Parameterization and validation","volume":"23","author":"Jakalian","year":"2002","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.bioorg.2026.109571_bb0235","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1021\/acs.jctc.9b00591","article-title":"ff19SB: amino-acid-specific protein backbone parameters trained against quantum mechanics energy surfaces in solution","volume":"16","author":"Tian","year":"2020","journal-title":"J. Chem. Theory Comput."},{"key":"10.1016\/j.bioorg.2026.109571_bb0240","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1021\/ct200909j","article-title":"Routine microsecond molecular dynamics simulations with AMBER on GPUs. 1. Generalized born","volume":"8","author":"Gotz","year":"2012","journal-title":"J. Chem. Theory Comput."},{"key":"10.1016\/j.bioorg.2026.109571_bb0245","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.jcmg.2012.01.017","article-title":"Molecular imaging of fibrin deposition in deep vein thrombosis using fibrin-targeted near-infrared fluorescence","volume":"5","author":"Hara","year":"2012","journal-title":"JACC Cardiovasc. Imaging"}],"container-title":["Bioorganic Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045206826001070?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045206826001070?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:11:57Z","timestamp":1770973917000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045206826001070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":49,"alternative-id":["S0045206826001070"],"URL":"https:\/\/doi.org\/10.1016\/j.bioorg.2026.109571","relation":{},"ISSN":["0045-2068"],"issn-type":[{"value":"0045-2068","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of selective NBD-based fluorescent probes for in vitro detection of factor XIa and Thrombus imaging","name":"articletitle","label":"Article Title"},{"value":"Bioorganic Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioorg.2026.109571","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"109571"}}