{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:52:01Z","timestamp":1791409921555,"version":"4.3.3"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioorganic Chemistry"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.bioorg.2026.110217","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T22:17:06Z","timestamp":1783203426000},"page":"110217","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Genome mining and metabolomics unveil new napyradiomycin antibiotics from Streptomyces sp. 0H2M"],"prefix":"10.1016","volume":"181","author":[{"given":"Min","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuo","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qichen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Benjamin","family":"Naman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bioorg.2026.110217_bb0005","doi-asserted-by":"crossref","first-page":"487","DOI":"10.7164\/antibiotics.39.487","article-title":"Novel antibiotics napyradiomycins production, isolation, physico-chemical properties and biological activity","volume":"39","author":"Shiomi","year":"1986","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.bioorg.2026.110217_bb0010","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00203-025-04291-6","article-title":"Insights for napyradiomycin family: structures, bioactivities and biosynthetic pathways","volume":"207","author":"Chen","year":"2025","journal-title":"Arch. Microbiol."},{"key":"10.1016\/j.bioorg.2026.110217_bb0015","doi-asserted-by":"crossref","first-page":"680","DOI":"10.3390\/md9040680","article-title":"Bactericidal kinetics of marine-derived Napyradiomycins against contemporary methicillin-resistant Staphylococcus aureus","volume":"9","author":"Haste","year":"2011","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.bioorg.2026.110217_bb0020","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1021\/acs.jnatprod.7b00211","article-title":"Identification of antiangiogenic potential and cellular mechanisms of napyradiomycin a1 isolated from the marine-derived Streptomyces sp. YP127","volume":"80","author":"Hwang","year":"2017","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.bioorg.2026.110217_bb0025","article-title":"Napyradiomycin A4 and its relate compounds, a new anti-prv agent and their antibacterial activities, from Streptomyces kebangsaanensis WS-68302","volume":"28","author":"Zhang","year":"2023","journal-title":"Molecules"},{"key":"10.1016\/j.bioorg.2026.110217_bb0030","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1128\/JB.188.4.1236-1244.2006","article-title":"Biosynthesis of a natural polyketide-isoprenoid hybrid compound, furaquinocin a: identification and heterologous expression of the gene cluster","volume":"188","author":"Kawasaki","year":"2006","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bioorg.2026.110217_bb0035","doi-asserted-by":"crossref","first-page":"16362","DOI":"10.1074\/jbc.M611046200","article-title":"Molecular basis for chloronium-mediated meroterpene cyclization: cloning, sequencing, and heterologous expression of the napyradiomycin biosynthetic gene cluster","volume":"282","author":"Winter","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bioorg.2026.110217_bb0040","doi-asserted-by":"crossref","first-page":"11009","DOI":"10.1002\/anie.201804351","article-title":"Total synthesis establishes the biosynthetic pathway to the naphterpin and marinone natural products","volume":"57","author":"Murray","year":"2018","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bioorg.2026.110217_bb0045","doi-asserted-by":"crossref","first-page":"17840","DOI":"10.1021\/jacs.8b10134","article-title":"Total enzyme syntheses of napyradiomycins A1 and B1","volume":"140","author":"McKinnie","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bioorg.2026.110217_bb0050","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1021\/acs.biochem.2c00338","article-title":"Structural basis of stereospecific vanadium-dependent haloperoxidase family enzymes in napyradiomycin biosynthesis","volume":"61","author":"Chen","year":"2022","journal-title":"Biochemistry"},{"issue":"3","key":"10.1016\/j.bioorg.2026.110217_bb0055","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1080\/01614940.2020.1823788","article-title":"Halogenases for biosynthetic pathway engineering: toward new routes to naturals and non-naturals","volume":"64","author":"Menon","year":"2020","journal-title":"Catal. Rev."},{"key":"10.1016\/j.bioorg.2026.110217_bb0060","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1039\/D1NP00032B","article-title":"Genome mining methods to discover bioactive natural products","volume":"38","author":"Bauman","year":"2021","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.bioorg.2026.110217_bb0065","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1128\/aem.63.8.3233-3241.1997","article-title":"Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients","volume":"63","author":"Heuer","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.bioorg.2026.110217_bb0070","doi-asserted-by":"crossref","first-page":"932","DOI":"10.2144\/03355bm05","article-title":"High yield preparation of genomic DNA from Streptomyces","volume":"35","author":"Nikodinovic","year":"2003","journal-title":"Biotechniques"},{"key":"10.1016\/j.bioorg.2026.110217_bb0075","doi-asserted-by":"crossref","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bioorg.2026.110217_bb0080","doi-asserted-by":"crossref","unstructured":"C. L. M. Gilchrist, Y.-H. Chooi, clinker & clustermap.Js: automatic generation of gene cluster comparison figures, Bioinformatics 37 (2021) 2473\u20132475, DOI:https:\/\/doi.org\/10.1093\/bioinformatics\/btab007.","DOI":"10.1093\/bioinformatics\/btab007"},{"key":"10.1016\/j.bioorg.2026.110217_bb0085","series-title":"Gaussian 9 Rev. E.01.Journal","author":"Frisch","year":"2013"},{"key":"10.1016\/j.bioorg.2026.110217_bb0090","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1021\/cr200106v","article-title":"Computational prediction of 1H and 13C chemical shifts: a useful tool for natural product, mechanistic, and synthetic organic chemistry","volume":"112","author":"Lodewyk","year":"2012","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bioorg.2026.110217_bb0095","doi-asserted-by":"crossref","first-page":"8544","DOI":"10.1021\/acs.joc.1c00987","article-title":"Sensitivity analysis of DP4+ with the probability distribution terms: development of a universal and customizable method","volume":"86","author":"Zanardi","year":"2021","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.bioorg.2026.110217_bb0100","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.bioorg.2026.110217_bb0105","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1016\/S0039-9140(97)00017-9","article-title":"A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability","volume":"44","author":"Ishiyama","year":"1997","journal-title":"Talanta"},{"key":"10.1016\/j.bioorg.2026.110217_bb0110","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4103\/1947-2714.100998","article-title":"Western blot: technique, theory, and trouble shooting","volume":"4","author":"Mahmood","year":"2012","journal-title":"N. Am. J. Med. Sci."},{"key":"10.1016\/j.bioorg.2026.110217_bb0115","doi-asserted-by":"crossref","first-page":"D678","DOI":"10.1093\/nar\/gkae1115","article-title":"MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration","volume":"53","author":"Zdouc","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bioorg.2026.110217_bb0120","doi-asserted-by":"crossref","first-page":"732","DOI":"10.7164\/antibiotics.40.732","article-title":"Studies on new antibiotics SF2415","volume":"40","author":"Shomura","year":"1987","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.bioorg.2026.110217_bb0125","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1021\/np058011z","article-title":"Antibiotic terpenoid chloro-dihydroquinones from a new marine actinomycete","volume":"68","author":"Soria-Mercado","year":"2005","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.bioorg.2026.110217_bb0130","doi-asserted-by":"crossref","first-page":"16362","DOI":"10.1074\/jbc.M611046200","article-title":"Molecular basis for chloronium-mediated meroterpene cyclization: cloning, sequencing, and heterologous expression of the napyradiomycin biosynthetic gene cluster","volume":"282","author":"Winter","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bioorg.2026.110217_bb0135","doi-asserted-by":"crossref","first-page":"4268","DOI":"10.1021\/ja201088k","article-title":"A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis","volume":"133","author":"Bernhardt","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bioorg.2026.110217_bb0140","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1039\/D0NP00018C","article-title":"Meroterpenoid natural products from Streptomyces bacteria - the evolution of chemoenzymatic syntheses","volume":"37","author":"Murray","year":"2020","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.bioorg.2026.110217_bb0145","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/s41589-021-00944-x","article-title":"Reaction pathway engineering converts a radical hydroxylase into a halogenase","volume":"18","author":"Neugebauer","year":"2022","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bioorg.2026.110217_bb0150","doi-asserted-by":"crossref","first-page":"17723","DOI":"10.1073\/pnas.0909649106","article-title":"Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2","volume":"106","author":"Matthews","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bioorg.2026.110217_bb0155","doi-asserted-by":"crossref","first-page":"4294","DOI":"10.1002\/anie.201306712","article-title":"Bioorganometallic chemistry with IspG and IspH: structure, function, and inhibition of the [Fe4S4] proteins involved in isoprenoid biosynthesis","volume":"53","author":"Wang","year":"2014","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bioorg.2026.110217_bb0160","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1093\/jnci\/51.5.1417","article-title":"In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors","volume":"51","author":"Giard","year":"1973","journal-title":"J. Natl. Cancer Inst."},{"key":"10.1016\/j.bioorg.2026.110217_bb0165","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1093\/jnci\/djq279","article-title":"Lung cancer cell lines as tools for biomedical discovery and research","volume":"102","author":"Gazdar","year":"2010","journal-title":"J. Natl. Cancer Inst."},{"key":"10.1016\/j.bioorg.2026.110217_bb0170","first-page":"171","article-title":"PARP cleavage as a means of assessing apoptosis","volume":"88","author":"Mullen","year":"2004","journal-title":"Methods Mol. Med."},{"key":"10.1016\/j.bioorg.2026.110217_bb0175","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1021\/acsmeasuresciau.1c00014","article-title":"Programmed cell-death mechanism analysis using same-cell, multimode DNA and proteoform electrophoresis","volume":"1","author":"Gomez Martinez","year":"2021","journal-title":"ACS Meas. Sci. Au."},{"key":"10.1016\/j.bioorg.2026.110217_bb0180","article-title":"Monitoring and measuring autophagy","volume":"18","author":"Streeter","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bioorg.2026.110217_bb0185","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/978-1-4939-2627-5_8","article-title":"LC3-II tagging and Western blotting for monitoring autophagic activity in mammalian cells","volume":"1303","author":"Streeter","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bioorg.2026.110217_bb0190","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/ja.2011.138","article-title":"Napyradiomycin A1, an inhibitor of mitochondrial complexes I and II","volume":"65","author":"Yamamoto","year":"2012","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.bioorg.2026.110217_bb0195","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1021\/np400466j","article-title":"Napyradiomycin derivatives, produced by a marine-derived actinomycete, illustrate cytotoxicity by induction of apoptosis","volume":"77","author":"Farnaes","year":"2014","journal-title":"J. Nat. Prod."}],"updated-by":[{"DOI":"10.1016\/j.bioorg.2026.110217","type":"removal","label":"Removal","source":"publisher","updated":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000}}],"container-title":["Bioorganic Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045206826007534?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045206826007534?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:17:13Z","timestamp":1791407833000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045206826007534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":39,"alternative-id":["S0045206826007534"],"URL":"https:\/\/doi.org\/10.1016\/j.bioorg.2026.110217","relation":{},"ISSN":["0045-2068"],"issn-type":[{"value":"0045-2068","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genome mining and metabolomics unveil new napyradiomycin antibiotics from Streptomyces sp. 0H2M","name":"articletitle","label":"Article Title"},{"value":"Bioorganic Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bioorg.2026.110217","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110217"}}