{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:00:39Z","timestamp":1784084439719,"version":"3.55.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"name":"Programa de Apoyo a Proyectos de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica, UNAM-DGAPA-PAPIIT","award":["IN204416"],"award-info":[{"award-number":["IN204416"]}]},{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda, CONACyT","award":["80811"],"award-info":[{"award-number":["80811"]}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","clinicalkey.com","em-consulte.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomedicine & Pharmacotherapy"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.biopha.2018.04.166","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T12:28:57Z","timestamp":1525696137000},"page":"1552-1556","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Spasmolytic effect of aqueous extract of Tagetes erecta L. flowers is mediated through calcium channel blockade on the guinea-pig ileum"],"prefix":"10.1016","volume":"103","author":[{"given":"Rosa","family":"Ventura-Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe E.","family":"\u00c1ngeles-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma Eva","family":"Gonz\u00e1lez-Trujano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrna","family":"D\u00e9ciga-Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biopha.2018.04.166_bib0005","series-title":"Cat\u00e1logo de nombres vulgares y cient\u00edficos de las plantas mexicanas","author":"Mart\u00ednez","year":"1979"},{"key":"10.1016\/j.biopha.2018.04.166_bib0010","series-title":"Biblioteca Digital de la Medicina Tradicional Mexicana","author":"Argueta","year":"2009"},{"key":"10.1016\/j.biopha.2018.04.166_bib0015","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.biopha.2017.06.064","article-title":"Preclinical evidence of the anxiolytic and sedative-like activities of Tagetes erecta L. reinforces its ethnobotanical approach","volume":"93","author":"P\u00e9rez-Ortega","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.biopha.2018.04.166_bib0020","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jep.2017.03.004","article-title":"Anthelmintic effect of Psidium guajava and Tagetes erecta on wild-type and Levamisole-resistant Caenorhabditis elegans strains","volume":"202","author":"Pi\u00f1a-V\u00e1zquez","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0025","first-page":"183","article-title":"A concise review on Tagetes erecta","volume":"3","author":"Gopi","year":"2012","journal-title":"Int. J. Phytopharm. Res."},{"key":"10.1016\/j.biopha.2018.04.166_bib0030","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1002\/ptr.2550","article-title":"Studies on the antioxidant and analgesic activities of Aztec marigold (Tagetes erecta) flowers","volume":"22","author":"Bashir","year":"2008","journal-title":"Phytother. Res."},{"key":"10.1016\/j.biopha.2018.04.166_bib0035","doi-asserted-by":"crossref","first-page":"386","DOI":"10.4103\/0253-7613.115026","article-title":"Antidepressant-like action of the hydromethanolic flower extract of Tagetes erecta L. in mice and its possible mechanism of action","volume":"45","author":"Khulbe","year":"2013","journal-title":"Indian. J. Pharmacol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0040","doi-asserted-by":"crossref","first-page":"325","DOI":"10.4314\/tjpr.v8i4.45224","article-title":"Antinociceptive and anti-inflammatory effects of solvent extracts of Tagetes erectus Linn (Asteraceae)","volume":"8","author":"Shinde","year":"2009","journal-title":"Trop. J. Pharm. Res."},{"key":"10.1016\/j.biopha.2018.04.166_bib0045","first-page":"133","article-title":"Antinociceptive and diuretic activities of Tagetes erecta Linn","volume":"2","author":"Hossain","year":"2014","journal-title":"World J. Pharm. Sci."},{"key":"10.1016\/j.biopha.2018.04.166_bib0050","first-page":"1412","article-title":"Studies on the chemical constituents from the stem and leaves of Tagetes erecta","volume":"33","author":"Zhang","year":"2010","journal-title":"Zhong Yao Cai"},{"key":"10.1016\/j.biopha.2018.04.166_bib0055","doi-asserted-by":"crossref","DOI":"10.1002\/cbdv.201600463","article-title":"Variation in phenolic profile, \u03b2\u2010carotene and flavonoid contents, biological activities of two Tagetes species from Pakistani flora","volume":"14","author":"Ayub","year":"2017","journal-title":"Chem. Biodivers."},{"key":"10.1016\/j.biopha.2018.04.166_bib0060","series-title":"An\u00e1lisis cuantitativo del conocimiento tradicional sobre plantas utilizadas para el tratamiento de enfermedades gastrointestinales en Zapotitl\u00e1n de las Salinas, Puebla. Interciencia","author":"Hern\u00e1ndez","year":"2005"},{"key":"10.1016\/j.biopha.2018.04.166_bib0065","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0944-7113(11)80050-4","article-title":"Activity of medicinal plants used to treat gastrointestinal and respiratory diseases in the highland of Chiapas","volume":"2","author":"Tortoriello","year":"1995","journal-title":"Phytomedicine"},{"key":"10.1016\/j.biopha.2018.04.166_bib0070","unstructured":"NOM-062-ZOO-1999, (1999) Official Mexican Norm for animal care and management. Secretar\u00eda de salud. Especificaciones t\u00e9cnicas para la producci\u00f3n, cuidado y uso de los animales de laboratorio. https:\/\/www.gob.mx\/senasica\/documentos\/nom-062-zoo-1999 (Accessed 27 September, 2017)."},{"key":"10.1016\/j.biopha.2018.04.166_bib0075","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jep.2016.12.023","article-title":"Spasmogenic and spasmolytic activities of Agastache mexicana ssp. mexicana and A. mexicana ssp. xolocotziana methanolic extracts on the guinea pig ileum","volume":"196","author":"Ventura-Mart\u00ednez","year":"2017","journal-title":"J. Ethnopharmacol"},{"key":"10.1016\/j.biopha.2018.04.166_bib0080","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0031-6997(24)01321-8","article-title":"Calcium movements, distribution and function in smooth muscle","volume":"49","author":"Karaki","year":"1997","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.biopha.2018.04.166_bib0085","first-page":"G849","article-title":"Signal-transduction pathway that regulate visceral smooth muscle function. III. Coupling of muscarinic receptors to signaling kinases and effector proteins in gastrointestinal smooth muscle","volume":"288","author":"Gerthoffer","year":"2005","journal-title":"Am. J. Physiol. Gastro. Liv. Physiol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0090","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/sj.bjp.0704914","article-title":"A possible role of neurotensin in NANC relaxation of longitudinal muscle of the jejunum and ileum of Wistar rats","volume":"137","author":"Yamaji","year":"2002","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0095","first-page":"G291","article-title":"Distinct roles of nitric oxide synthases and interstitial cells of Cajal in rectoanal relaxation","volume":"289","author":"Terauchi","year":"2005","journal-title":"Am. J. Physiol. Gastro. Liv. Physiol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0100","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ejphar.2015.10.007","article-title":"Flavonoid galetin 3,6-dimethyl ether attenuates guinea pig ileum contraction through K++ channel activation and decrease in cytosolic calcium concentration","volume":"767","author":"Vasconcenlos","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0105","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.jep.2011.09.019","article-title":"Gastrointestinal and respiratory activities of Acacia leucophloea","volume":"138","author":"Imran","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0110","first-page":"103","article-title":"Phytochemicals and their biological activities of plants in Tagetes L","volume":"4","author":"Xu","year":"2012","journal-title":"Chin. Herb. Med."},{"key":"10.1016\/j.biopha.2018.04.166_bib0115","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.seppur.2009.11.024","article-title":"Optimization of supercritical carbon dioxide extraction of lutein esters from marigold (Tagetes erecta L.) with vegetable oils as continuous co-solvents","volume":"71","author":"Gao","year":"2009","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0120","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1159\/000074237","article-title":"Inhibition of guinea pig intestinal peristalsis by the flavonoids quercetin, naringenin, apigenin and genistein","volume":"70","author":"Gharzouli","year":"2004","journal-title":"Pharmacology"},{"key":"10.1016\/j.biopha.2018.04.166_bib0125","first-page":"582","article-title":"Investigation of the spasmolytic activity of the flavonoid fraction of Achillea millefolium s. l. on isolated guinea-pig ilea","volume":"56","author":"Lemmens-Gruber","year":"2006","journal-title":"Arzneimittelforschung"},{"key":"10.1016\/j.biopha.2018.04.166_bib0130","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1159\/000233423","article-title":"Quercetin inhibits anaphylactic contraction of guinea pig ileum smooth muscle","volume":"71","author":"Fanning","year":"1983","journal-title":"Int. Arch. Allergy Immunol."},{"key":"10.1016\/j.biopha.2018.04.166_bib0135","first-page":"199","article-title":"The involvement of L-type voltage operated calcium channels in the vascular effect of quercetin in male rats","volume":"5","author":"Roghani","year":"2006","journal-title":"Iran J. Pharm. Res."},{"key":"10.1016\/j.biopha.2018.04.166_bib0140","first-page":"1247","article-title":"Phytochemical evaluation of Lythrum salicaria extracts and their effects on guinea-pig ileum","volume":"8","author":"Bencsik","year":"2013","journal-title":"Nat. Prod. Commun."},{"key":"10.1016\/j.biopha.2018.04.166_bib0145","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1002\/ptr.3634","article-title":"Gastrointestinal stimulant effect of Urginea indica Kunth and involvement of muscarinic receptors","volume":"26","author":"Abbas","year":"2012","journal-title":"Phytother. Res."},{"key":"10.1016\/j.biopha.2018.04.166_bib0150","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s12906-017-1616-4","article-title":"Spasmogenic and spasmolytic activity of rind of Punica granatum Linn","volume":"17","author":"Ali","year":"2017","journal-title":"BMC Complement Altern. Med."},{"key":"10.1016\/j.biopha.2018.04.166_bib0155","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2015.05.047","article-title":"Effects of the aqueous extract of Pittosporum mannii Hook. f. (Pittosporaceae) stem barks on spontaneous and spasmogen-induced contractile activity of isolated rat duodenum","volume":"172","author":"Joseph","year":"2015","journal-title":"J. Ethnopharmacol."}],"container-title":["Biomedicine & Pharmacotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0753332217367872?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0753332217367872?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T08:23:54Z","timestamp":1751617434000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0753332217367872"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":31,"alternative-id":["S0753332217367872"],"URL":"https:\/\/doi.org\/10.1016\/j.biopha.2018.04.166","relation":{},"ISSN":["0753-3322"],"issn-type":[{"value":"0753-3322","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Spasmolytic effect of aqueous extract of Tagetes erecta L. flowers is mediated through calcium channel blockade on the guinea-pig ileum","name":"articletitle","label":"Article Title"},{"value":"Biomedicine & Pharmacotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biopha.2018.04.166","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Masson SAS. All rights reserved.","name":"copyright","label":"Copyright"}]}}