{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:05:12Z","timestamp":1793030712012,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003086","name":"Basque Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","em-consulte.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomedicine & Pharmacotherapy"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.biopha.2026.119677","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:52:39Z","timestamp":1781661159000},"page":"119677","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Tailoring PRGF fibrin membranes: A simple approach to enhance structural and functional properties for wound healing"],"prefix":"10.1016","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8386-5303","authenticated-orcid":false,"given":"Eduardo","family":"Anitua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-0505","authenticated-orcid":false,"given":"Mar\u00eda","family":"Troya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8458-6042","authenticated-orcid":false,"given":"Mar","family":"Zalduendo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8940-0953","authenticated-orcid":false,"given":"Iraia","family":"Reparaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4422-4527","authenticated-orcid":false,"given":"Mohammad H.","family":"Alkhraisat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biopha.2026.119677_bib1","article-title":"Advances in corneal tissue engineering: comparative performance of bioengineered grafts in animal models","volume":"62","author":"Anitua","year":"2025","journal-title":"Med. (Kaunas. )"},{"key":"10.1016\/j.biopha.2026.119677_bib2","article-title":"Biomaterials for corneal regeneration","volume":"12","author":"Li","year":"2025","journal-title":"Adv. Sci. (Weinh. )"},{"key":"10.1016\/j.biopha.2026.119677_bib3","article-title":"Ocular-surface regeneration therapies for eye disorders: the state of the art","volume":"12","author":"Posarelli","year":"2023","journal-title":"Biotech. (Basel (Switz. ))"},{"key":"10.1016\/j.biopha.2026.119677_bib4","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1297357","article-title":"New strategy of personalized tissue regeneration: when autologous platelet concentrates encounter biomaterials","volume":"11","author":"Ren","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.biopha.2026.119677_bib5","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1080\/14712598.2021.1936495","article-title":"Platelet-rich therapies as an emerging platform for regenerative medicine","volume":"21","author":"Orive","year":"2021","journal-title":"Expert. Opin. Biol. Ther."},{"key":"10.1016\/j.biopha.2026.119677_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms262211097","article-title":"Blood derivatives in the therapy of ocular surface diseases","volume":"26","author":"St\u0119pie\u0144","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biopha.2026.119677_bib7","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1167\/tvst.12.11.32","article-title":"Biological and adhesive properties of an autologous protein-based fibrin sealant for ophthalmological applications","volume":"12","author":"Anitua","year":"2023","journal-title":"Transl. Vis. Sci. Technol."},{"key":"10.1016\/j.biopha.2026.119677_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1399130","article-title":"Immunoregulatory role of platelet derivatives in the macrophage-mediated immune response","volume":"15","author":"Anitua","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.biopha.2026.119677_bib9","doi-asserted-by":"crossref","DOI":"10.3390\/jcm10235711","article-title":"Membrane of plasma rich in growth factors in primary pterygium surgery compared to amniotic membrane transplantation and conjunctival autograft","volume":"10","author":"Idoipe","year":"2021","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.biopha.2026.119677_bib10","article-title":"Plasma rich in growth factors membrane as coadjuvant treatment in the surgery of ocular surface disorders","volume":"97","author":"Sanchez-Avila","year":"2018","journal-title":"Med. (Baltim. )"},{"key":"10.1016\/j.biopha.2026.119677_bib11","doi-asserted-by":"crossref","first-page":"293","DOI":"10.22336\/rjo.2021.60","article-title":"Use of plasma rich in growth factors (PRGF-Endoret(R)) fibrin membrane to cover corneal dellen","volume":"65","author":"Rahhal-Ortuno","year":"2021","journal-title":"Rom. J. Ophthalmol."},{"key":"10.1016\/j.biopha.2026.119677_bib12","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1002\/jbm.a.37579","article-title":"Cellular composition modifies the biological properties and stability of platelet rich plasma membranes for tissue engineering","volume":"111","author":"Anitua","year":"2023","journal-title":"J. Biomed. Mater. Res. A"},{"key":"10.1016\/j.biopha.2026.119677_bib13","doi-asserted-by":"crossref","DOI":"10.3390\/biom12091215","article-title":"Differential protein content between fresh and freeze-dried plasma rich in growth factors eye drops","volume":"12","author":"Anitua","year":"2022","journal-title":"Biomolecules"},{"key":"10.1016\/j.biopha.2026.119677_bib14","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1016\/j.jmbbm.2011.06.013","article-title":"Investigating a novel nanostructured fibrin-agarose biomaterial for human cornea tissue engineering: rheological properties","volume":"4","author":"Ionescu","year":"2011","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"10.1016\/j.biopha.2026.119677_bib15","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.jtha.2025.09.013","article-title":"Standardization of scanning electron microscopy analysis of fibrin fiber diameter measurement: communication from the ISTH SSC subcommittee on FXIII and fibrinogen","volume":"24","author":"Cai","year":"2026","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.biopha.2026.119677_bib16","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3109\/09537104.2016.1143921","article-title":"Implementation of a more physiological plasma rich in growth factor (PRGF) protocol: anticoagulant removal and reduction in activator concentration","volume":"27","author":"Anitua","year":"2016","journal-title":"Platelets"},{"key":"10.1016\/j.biopha.2026.119677_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/jfb16090328","article-title":"Recent advances in 3D bioprinting of porous scaffolds for tissue engineering: a narrative and critical review","volume":"16","author":"Picado-Tejero","year":"2025","journal-title":"J. Funct. Biomater."},{"key":"10.1016\/j.biopha.2026.119677_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms26020721","article-title":"The effect of long-term cryopreservation on the properties and functionality of platelet-rich plasma","volume":"26","author":"Beitia","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.biopha.2026.119677_bib19","doi-asserted-by":"crossref","first-page":"525","DOI":"10.2217\/rme-2018-0119","article-title":"Freeze-dried versus fresh platelet-rich plasma in acute wound healing of an animal model","volume":"14","author":"Huber","year":"2019","journal-title":"Regen. Med."},{"key":"10.1016\/j.biopha.2026.119677_bib20","first-page":"44903","article-title":"The impact of storage protocols on the mechanical and structural properties of frozen platelet-rich fibrin membranes","volume":"12","author":"Gottumukkala","year":"2025","journal-title":"Biomater. Investig. Dent."},{"key":"10.1016\/j.biopha.2026.119677_bib21","article-title":"Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source","volume":"31","author":"Akbaripour","year":"2026","journal-title":"Regen. Ther."},{"key":"10.1016\/j.biopha.2026.119677_bib22","article-title":"Advanced corneal hydrogels: from passive replacement to active regeneration and intelligent interaction","author":"Wang","year":"2026","journal-title":"Adv. Sci. (Weinh. )"},{"key":"10.1016\/j.biopha.2026.119677_bib23","doi-asserted-by":"crossref","DOI":"10.1038\/s41536-026-00471-0","article-title":"Seeing through collagen: integrative pro-regenerative corneal implants for clearer future","author":"Huang","year":"2026","journal-title":"NPJ Regen. Med."},{"key":"10.1016\/j.biopha.2026.119677_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0131989","article-title":"Corneal regeneration by deep anterior lamellar keratoplasty (DALK) using decellularized corneal matrix","volume":"10","author":"Hashimoto","year":"2015","journal-title":"PLoS. One"},{"key":"10.1016\/j.biopha.2026.119677_bib25","first-page":"673","article-title":"Effect of pores on transmission properties of transparent ceramics","volume":"5","author":"Li","year":"2011","journal-title":"Optoelectron. Adv. Mater."},{"key":"10.1016\/j.biopha.2026.119677_bib26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.3390\/ceramics7010025","article-title":"Beyond SCanning Electron Microscopy: Comprehensive Pore Analysis in Transparent Ceramics Using Optical Microscopy","volume":"7","author":"Picelli","year":"2024","journal-title":"Ceramics"},{"key":"10.1016\/j.biopha.2026.119677_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/jfb10030030","article-title":"Strategies to tune electrospun scaffold porosity for effective cell response in tissue engineering","volume":"10","author":"Ameer","year":"2019","journal-title":"J. Funct. Biomater."},{"key":"10.1016\/j.biopha.2026.119677_bib28","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1002\/app.32608","article-title":"Effect of fiber orientation on pore size characteristics of nonwoven structures","volume":"118","author":"Rawal","year":"2010","journal-title":"J. Appl. Polym. Sci."},{"key":"10.1016\/j.biopha.2026.119677_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/315397","article-title":"Effect of pore size on the biodegradation rate of silk fibroin scaffolds","volume":"2015","author":"Luo","year":"2015","journal-title":"Adv. Mater. Sci. Eng."},{"key":"10.1016\/j.biopha.2026.119677_bib30","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1161\/ATVBAHA.110.213389","article-title":"Molecular mechanisms affecting fibrin structure and stability","volume":"31","author":"Lord","year":"2011","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.biopha.2026.119677_bib31","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1038\/s41598-024-52844-4","article-title":"Internal fibrinolysis of fibrin clots is driven by pore expansion","volume":"14","author":"Risman","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biopha.2026.119677_bib32","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1073\/pnas.1221602110","article-title":"Heparin-binding domain of fibrin(ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix","volume":"110","author":"Martino","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.biopha.2026.119677_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/biom16020335","article-title":"Fibrinogen and fibrin as growth factor regulators: pathological implications, and translational opportunities","volume":"16","author":"Sahni","year":"2026","journal-title":"Biomolecules"},{"key":"10.1016\/j.biopha.2026.119677_bib34","doi-asserted-by":"crossref","first-page":"e605","DOI":"10.1111\/aos.12710","article-title":"Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies","volume":"93","author":"Anitua","year":"2015","journal-title":"Acta Ophthalmol."},{"key":"10.1016\/j.biopha.2026.119677_bib35","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1038\/s41563-025-02300-z","article-title":"Adaptive peptide dispersions enable drying-induced biomolecule encapsulation","volume":"24","author":"Dave","year":"2025","journal-title":"Nat. Mater."},{"key":"10.1016\/j.biopha.2026.119677_bib36","doi-asserted-by":"crossref","DOI":"10.3390\/foods13172790","article-title":"Influence of liquid nitrogen pre-freezing and drying methods on the collagen content, physical properties, and flavor of fish swim bladder","volume":"13","author":"Dong","year":"2024","journal-title":"Foods"},{"key":"10.1016\/j.biopha.2026.119677_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.powtec.2024.120012","article-title":"Compaction behavior of freeze-dried and spray-dried trypsin\/lactose particulate systems","volume":"445","author":"Zhang","year":"2024","journal-title":"Powder Technol."},{"key":"10.1016\/j.biopha.2026.119677_bib38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.preteyeres.2015.07.002","article-title":"Progress in corneal wound healing","volume":"49","author":"Ljubimov","year":"2015","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.biopha.2026.119677_bib39","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.stem.2022.07.006","article-title":"Wound healing, fibroblast heterogeneity, and fibrosis","volume":"29","author":"Talbott","year":"2022","journal-title":"Cell. Stem Cell."},{"key":"10.1016\/j.biopha.2026.119677_bib40","doi-asserted-by":"crossref","DOI":"10.3390\/biom13060945","article-title":"Fibroblasts-warriors at the intersection of wound healing and disrepair","volume":"13","author":"Roman","year":"2023","journal-title":"Biomolecules"},{"key":"10.1016\/j.biopha.2026.119677_bib41","article-title":"Coupling of fibrin reorganization and fibronectin patterning by corneal fibroblasts in response to PDGF BB and TGFbeta1","volume":"7","author":"Miron-Mendoza","year":"2020","journal-title":"Bioeng. (Basel)"},{"key":"10.1016\/j.biopha.2026.119677_bib42","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1046\/j.0022-202x.2001.01551.x","article-title":"Fibrinogen and fibrin are anti-adhesive for keratinocytes: a mechanism for fibrin eschar slough during wound repair","volume":"117","author":"Kubo","year":"2001","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.biopha.2026.119677_bib43","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.916187","article-title":"Fibrin(ogen) is constitutively expressed by differentiated intestinal epithelial cells and mediates wound healing","volume":"13","author":"Seltana","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.biopha.2026.119677_bib44","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2024.1444986","article-title":"Optimizing scaffold pore size for tissue engineering: insights across various tissue types","volume":"12","author":"Mukasheva","year":"2024","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.biopha.2026.119677_bib45","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.1021\/bm0602233","article-title":"Interpenetrating collagen-fibrin composite matrices with varying protein contents and ratios","volume":"7","author":"Rowe","year":"2006","journal-title":"Biomacromolecules"}],"container-title":["Biomedicine & Pharmacotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0753332226007134?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0753332226007134?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T06:46:47Z","timestamp":1784530007000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0753332226007134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":45,"alternative-id":["S0753332226007134"],"URL":"https:\/\/doi.org\/10.1016\/j.biopha.2026.119677","relation":{},"ISSN":["0753-3322"],"issn-type":[{"value":"0753-3322","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Tailoring PRGF fibrin membranes: A simple approach to enhance structural and functional properties for wound healing","name":"articletitle","label":"Article Title"},{"value":"Biomedicine & Pharmacotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biopha.2026.119677","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"119677"}}