{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:51:55Z","timestamp":1791377515599,"version":"4.3.2"},"reference-count":110,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education of the Republic of Poland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024002","name":"Faculty of Veterinary Medicine, Damanhour University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","em-consulte.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biomedicine & Pharmacotherapy"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.biopha.2026.119680","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T21:58:52Z","timestamp":1781733532000},"page":"119680","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Anxiety-related platform in zebrafish (Danio rerio) larvae: An integrated analysis of behavioural and molecular assay in drug screening research"],"prefix":"10.1016","volume":"201","author":[{"given":"Ma\u0142gorzata","family":"Potoczna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0242-2587","authenticated-orcid":false,"given":"Natalia","family":"Kasica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Chmielewska-Krzesi\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Dybalska-Szczepanek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"W\u0105sowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Soko\u0142owska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9162-5449","authenticated-orcid":false,"given":"Piotr","family":"Podlasz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biopha.2026.119680_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.neubiorev.2022.104679","article-title":"Modeling neurodegenerative disorders in zebrafish","volume":"138","author":"Bashirzade","year":"2022","journal-title":"Neurosci. Biobehav. Rev."},{"issue":"8","key":"10.1016\/j.biopha.2026.119680_bib2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1038\/s41573-021-00210-8","article-title":"Zebrafish disease models in drug discovery: from preclinical modelling to clinical trials","volume":"20","author":"Patton","year":"2021","journal-title":"Nat. Rev. Drug. Discov."},{"key":"10.1016\/j.biopha.2026.119680_bib3","article-title":"Zebrafish as an Integrative model for central nervous system research: current advances and translational perspectives","author":"Pansera","year":"2025","journal-title":"Life (Vol. 15). Multidiscip. Digit. Publ. Inst. (MDPI)"},{"issue":"17","key":"10.1016\/j.biopha.2026.119680_bib4","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1002\/cne.23131","article-title":"Galanin gene expression and effects of its knock-down on the development of the nervous system in larval zebrafish","volume":"520","author":"Podlasz","year":"2012","journal-title":"J. Comp. Neurol."},{"key":"10.1016\/j.biopha.2026.119680_bib5","article-title":"Zebrafish: a versatile and powerful model for biomedical research","author":"Siddiqui","year":"2025","journal-title":"BioEssays (Vol. 47). John Wiley Sons Inc"},{"issue":"4","key":"10.1016\/j.biopha.2026.119680_bib6","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1089\/zeb.2014.0976","article-title":"Growth and maturation in the zebrafish, Danio rerio: a staging tool for teaching and research","volume":"11","author":"Singleman","year":"2014","journal-title":"Zebrafish"},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib7","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/jat.4059","article-title":"Aconitine disrupts serotonin neurotransmission via 5-hydroxytryptamine receptor in zebrafish embryo","volume":"41","author":"Chen","year":"2021","journal-title":"J. Appl. Toxicol. JAT"},{"key":"10.1016\/j.biopha.2026.119680_bib8","first-page":"190","article-title":"Role of gabra2, GABAA receptor alpha-2 subunit, in CNS development","volume":"3","author":"Gonzalez-Nunez","year":"2015","journal-title":"Biochem. Biophys. Rep."},{"key":"10.1016\/j.biopha.2026.119680_bib9","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.pbb.2014.09.022","article-title":"Serotonin antagonists induce anxiolytic and anxiogenic-like behavior in zebrafish in a receptor-subtype dependent manner","volume":"126","author":"Nowicki","year":"2014","journal-title":"Pharmacol. Biochem. Behav."},{"issue":"15\u201316","key":"10.1016\/j.biopha.2026.119680_bib10","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1007\/s00213-016-4352-4","article-title":"Abuse potential of methylenedioxymethamphetamine (MDMA) and its derivatives in zebrafish: role of serotonin 5HT2-type receptors","volume":"233","author":"Ponzoni","year":"2016","journal-title":"Psychopharmacology"},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib11","doi-asserted-by":"crossref","first-page":"6242","DOI":"10.1038\/s41598-021-84646-3","article-title":"Characterization of zebrafish GABAA receptor subunits","volume":"11","author":"Sadamitsu","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biopha.2026.119680_bib12","article-title":"A high-throughput system for drug screening based on the movement analysis of zebrafish","volume":"10","author":"Zhao","year":"2024","journal-title":"Heliyon"},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/bdrc.20212","article-title":"The developing zebrafish (Danio rerio): a vertebrate model for high-throughput screening of chemical libraries","volume":"93","author":"Lessman","year":"2011","journal-title":"Birth Defects Res. Part. C. Embryo Today. Rev."},{"key":"10.1016\/j.biopha.2026.119680_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.ynstr.2021.100405","article-title":"Understanding how stress responses and stress-related behaviors have evolved in zebrafish and mammals","volume":"15","author":"de Abreu","year":"2021","journal-title":"Neurobiol. Stress."},{"key":"10.1016\/j.biopha.2026.119680_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.pbb.2021.173205","article-title":"Unconventional anxiety pharmacology in zebrafish: drugs beyond traditional anxiogenic and anxiolytic spectra","volume":"207","author":"de Abreu","year":"2021","journal-title":"Pharmacol. Biochem. Behav."},{"key":"10.1016\/j.biopha.2026.119680_bib16","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.833227","article-title":"Standardizing zebrafish behavioral paradigms across life stages: an effort towards translational pharmacology","volume":"13","author":"Petersen","year":"2022","journal-title":"Front. Pharmacol."},{"issue":"12","key":"10.1016\/j.biopha.2026.119680_bib17","first-page":"6647","article-title":"Zebrafish larvae behavior models as a tool for drug screenings and pre-clinical trials: a review","volume":"23","author":"Rosa","year":"2022","journal-title":"Int. J. Mol. Sci. 2022 Vol. 23 Page 6647"},{"key":"10.1016\/j.biopha.2026.119680_bib18","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2022.900223","article-title":"Osmotic stress uncovers correlations and dissociations between larval zebrafish anxiety endophenotypes","volume":"15","author":"Cheng","year":"2022","journal-title":"Front. Mol. Neurosci."},{"issue":"8","key":"10.1016\/j.biopha.2026.119680_bib19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0181010","article-title":"Impact of stress, fear and anxiety on the nociceptive responses of larval zebrafish","volume":"12","author":"Lopez-Luna","year":"2017","journal-title":"PloS. One"},{"key":"10.1016\/j.biopha.2026.119680_bib20","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2022.900213","article-title":"Larval zebrafish as a model for mechanistic discovery in mental health","author":"Tan","year":"2022","journal-title":"Front. Mol. Neurosci. (Vol. 15). Frontiers Media S. A"},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3390\/endocrines6010011","article-title":"The Potential of zebrafish larvae and water vortex protocols in stress biology","volume":"6","author":"De Marco","year":"2025","journal-title":"Endocrines"},{"key":"10.1016\/j.biopha.2026.119680_bib22","unstructured":"Depression and other common mental disorders: global health estimates. (n.d.). Retrieved June 20, 2023, from \u3008https:\/\/apps.who.int\/iris\/handle\/10665\/254610\u3009."},{"issue":"5","key":"10.1016\/j.biopha.2026.119680_bib23","article-title":"Young adult depression, antidepressant prescriptions, and therapy intensification in people with incident depression in the united States","volume":"24","author":"Dibato","year":"2022","journal-title":"Prim. Care Companion CNS Disord."},{"issue":"2","key":"10.1016\/j.biopha.2026.119680_bib24","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4068\/cmj.2018.54.2.101","article-title":"Addressing the side effects of contemporary antidepressant drugs: a comprehensive review","volume":"54","author":"Wang","year":"2018","journal-title":"Chonnam Med. J."},{"issue":"2","key":"10.1016\/j.biopha.2026.119680_bib25","doi-asserted-by":"crossref","first-page":"131","DOI":"10.5455\/msm.2020.32.131-134","article-title":"Assessment of the antidepressant side effects occurrence in patients treated in primary care","volume":"32","author":"Ramic","year":"2020","journal-title":"Mater. Socio-Med."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib26","doi-asserted-by":"crossref","DOI":"10.1038\/s41398-021-01533-1","article-title":"Antidepressant side effects and their impact on treatment outcome in people with major depressive disorder: an iSPOT-D report","volume":"11","author":"Braund","year":"2021","journal-title":"Transl. Psychiatry"},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib27","doi-asserted-by":"crossref","DOI":"10.1111\/inm.13107","article-title":"The experience of \u201ctreatment-resistant\u201d depression: a qualitative meta-synthesis","volume":"32","author":"Crowe","year":"2023","journal-title":"Int. J. Ment. Health Nurs."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib28","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/jnc.15509","article-title":"From psychiatry to neurology: psychedelics as prospective therapeutics for neurodegenerative disorders","volume":"162","author":"Kozlowska","year":"2022","journal-title":"J. Neurochem."},{"issue":"14","key":"10.1016\/j.biopha.2026.119680_bib29","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1503\/cmaj.141124","article-title":"Psychedelic medicine: a re-emerging therapeutic paradigm","volume":"187","author":"Tupper","year":"2015","journal-title":"CMAJ Can. Med. Assoc. J."},{"issue":"7","key":"10.1016\/j.biopha.2026.119680_bib30","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S2215-0366(16)30065-7","article-title":"Psilocybin with psychological support for treatment-resistant depression: an open-label feasibility study","volume":"3","author":"Carhart-Harris","year":"2016","journal-title":"Lancet Psychiatry"},{"key":"10.1016\/j.biopha.2026.119680_bib31","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1056\/NEJMoa2206443","article-title":"Single-dose psilocybin for a treatment-resistant episode of major depression","volume":"387","author":"Goodwin","year":"2022","journal-title":"New. Engl. J. Med."},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib32","first-page":"734","article-title":"Potential therapeutic effects of psilocybin","volume":"14","author":"Johnson","year":"2017","journal-title":"Neurother. J. Am. Soc. Exp. Neurother."},{"key":"10.1016\/j.biopha.2026.119680_bib33","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1038\/s41386-025-02091-6","article-title":"Rapid and sustained antidepressant effects of vaporized N,N-dimethyltryptamine: a phase 2a clinical trial in treatment-resistant depression","volume":"50","author":"Falchi-Carvalho","year":"2025","journal-title":"Neuropsychopharmacology"},{"issue":"8","key":"10.1016\/j.biopha.2026.119680_bib34","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.2174\/092986709787581851","article-title":"Agonist-trafficking and hallucinogens","volume":"16","author":"Gonzalez-Maeso","year":"2009","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.biopha.2026.119680_bib35","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1001\/jamapsychiatry.2024.2546","article-title":"Adverse events in studies of classic psychedelics: a systematic review and meta-analysis","volume":"81","author":"Hinkle","year":"2024","journal-title":"JAMA Psychiatry"},{"issue":"4","key":"10.1016\/j.biopha.2026.119680_bib36","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1177\/2045125314527985","article-title":"Classical hallucinogens as antidepressants? A review of pharmacodynamics and putative clinical roles","volume":"4","author":"Baumeister","year":"2014","journal-title":"Ther. Adv. Psychopharmacol."},{"issue":"7842","key":"10.1016\/j.biopha.2026.119680_bib37","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/s41586-020-3008-z","article-title":"A non-hallucinogenic psychedelic analogue with therapeutic potential","volume":"589","author":"Cameron","year":"2020","journal-title":"Nature"},{"issue":"6579","key":"10.1016\/j.biopha.2026.119680_bib38","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1126\/science.abl8615","article-title":"Structure-based discovery of nonhallucinogenic psychedelic analogs","volume":"375","author":"Cao","year":"2022","journal-title":"Science (New York N. Y.)"},{"key":"10.1016\/j.biopha.2026.119680_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.ntt.2023.107163","article-title":"Inconsistencies in variable reporting and methods in larval zebrafish behavioral assays","volume":"96","author":"Hill","year":"2023","journal-title":"Neurotoxicol. Teratol."},{"key":"10.1016\/j.biopha.2026.119680_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.pnpbp.2024.111084","article-title":"A unified approach to investigating 4 dpf zebrafish larval behaviour through a standardised light\/dark assay","volume":"134","author":"Hillman","year":"2024","journal-title":"Progress. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"10.1016\/j.biopha.2026.119680_bib41","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2022.827282","article-title":"Detailed analysis of zebrafish larval behaviour in the light dark challenge assay shows that diel hatching time determines individual variation","volume":"13","author":"Rock","year":"2022","journal-title":"Front. Physiol."},{"key":"10.1016\/j.biopha.2026.119680_bib42","doi-asserted-by":"crossref","DOI":"10.3390\/toxics13090727","article-title":"Utilizing multiple behavioral endpoints to identify negative control chemicals in a larval zebrafish behavior assay","volume":"13","author":"Knapp","year":"2025","journal-title":"Toxics"},{"key":"10.1016\/j.biopha.2026.119680_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.namjnl.2025.100033","article-title":"A data analysis approach for locomotor behavioral responses in developmentally exposed zebrafish","volume":"1","author":"Rowson","year":"2025","journal-title":"NAM J."},{"key":"10.1016\/j.biopha.2026.119680_bib44","author":"Test No. 236: Fish Embryo Acute Toxicity (FET) Test","year":"2013","journal-title":"OECD Guidelines for the Testing of Chemicals, Section 2"},{"key":"10.1016\/j.biopha.2026.119680_bib45","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.pbb.2016.03.010","article-title":"Anxiety-related behavioral responses of pentylenetetrazole-treated zebrafish larvae to light-dark transitions","volume":"145","author":"Peng","year":"2016","journal-title":"Pharmacol. Biochem. Behav."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib46","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bbr.2011.03.025","article-title":"Patterns of avoidance behaviours in the light\/dark preference test in young juvenile zebrafish: a pharmacological study","volume":"222","author":"Steenbergen","year":"2011","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.biopha.2026.119680_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2022.133863","article-title":"Getting more out of the zebrafish light dark transition test","volume":"295","author":"Haigis","year":"2022","journal-title":"Chemosphere"},{"key":"10.1016\/j.biopha.2026.119680_bib48","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1007\/s00213-020-05642-5","article-title":"Screening selected medicinal plants for potential anxiolytic activity using an in vivo zebrafish model","volume":"237","author":"Maphanga","year":"2020","journal-title":"Psychopharmacology"},{"key":"10.1016\/j.biopha.2026.119680_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.134490","article-title":"DMSO effects larval zebrafish (Danio rerio) behavior, with additive and interaction effects when combined with positive controls","volume":"709","author":"Christou","year":"2020","journal-title":"Sci. Total. Environ."},{"key":"10.1016\/j.biopha.2026.119680_bib50","doi-asserted-by":"crossref","DOI":"10.3389\/ftox.2021.804033","article-title":"DMSO concentrations up to 1% are safe to be used in the zebrafish embryo developmental toxicity assay","volume":"3","author":"Hoyberghs","year":"2021","journal-title":"Front. Toxicol."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib51","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bbr.2011.03.025","article-title":"Patterns of avoidance behaviours in the light\/dark preference test in young juvenile zebrafish: a pharmacological study","volume":"222","author":"Steenbergen","year":"2011","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.biopha.2026.119680_bib52","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2021.112966","article-title":"Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos\/larvae (Danio rerio)","volume":"228","author":"Huang","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.biopha.2026.119680_bib53","doi-asserted-by":"crossref","DOI":"10.1016\/j.taap.2024.116809","article-title":"Xanthohumol, a prenylated flavonoid from hops (Humulus lupulus L.) exerts multidirectional pro-healing properties towards damaged zebrafish hair cells by regulating the innate immune response","volume":"483","author":"Kasica","year":"2024","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"12","key":"10.1016\/j.biopha.2026.119680_bib54","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1021\/acschemneuro.8b00239","article-title":"Neuroanatomical localization of galanin in zebrafish telencephalon and anticonvulsant effect of galanin overexpression","volume":"9","author":"Podlasz","year":"2018","journal-title":"ACS Chem. Neurosci."},{"key":"10.1016\/j.biopha.2026.119680_bib55","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinforma."},{"key":"10.1016\/j.biopha.2026.119680_bib56","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-1-60761-922-2_3","article-title":"Modeling stress and anxiety in zebrafish","volume":"52","author":"Cachat","year":"2011","journal-title":"Neuromethods"},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib57","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-019-0629-6","article-title":"Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish","volume":"2","author":"Brun","year":"2019","journal-title":"Commun. Biol."},{"key":"10.1016\/j.biopha.2026.119680_bib58","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines11102821","article-title":"Essential oils produce developmental toxicity in zebrafish embryos and cause behavior changes in zebrafish larvae","volume":"11","author":"da Silva","year":"2023","journal-title":"Biomedicines"},{"key":"10.1016\/j.biopha.2026.119680_bib59","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuro.2025.103371","article-title":"Power calculations for larval zebrafish in light-dark transition test for developmental neurotoxicity","volume":"112","author":"Konrad","year":"2026","journal-title":"NeuroToxicology"},{"key":"10.1016\/j.biopha.2026.119680_bib60","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s43440-020-00136-9","article-title":"Xanthotoxin reverses Parkinson\u2019s disease-like symptoms in zebrafish larvae and mice models: a comparative study","volume":"73","author":"Kozio\u0142","year":"2021","journal-title":"Pharmacol. Rep."},{"key":"10.1016\/j.biopha.2026.119680_bib61","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2015.00436","article-title":"The basal NPO crh fluctuation is sustained under compromised glucocorticoid signaling in diurnal zebrafish","volume":"9","author":"Yeh","year":"2015","journal-title":"Front. Neurosci."},{"issue":"12","key":"10.1016\/j.biopha.2026.119680_bib62","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23126647","article-title":"Zebrafish larvae behavior models as a tool for drug screenings and pre-clinical trials: a review","volume":"23","author":"Rosa","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"10.1016\/j.biopha.2026.119680_bib63","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1039\/D4FO03932G","article-title":"From in silico screening to in vivo validation in zebrafish - a framework for reeling in the right psychobiotics","volume":"16","author":"Valderrama","year":"2025","journal-title":"Food Funct."},{"issue":"2","key":"10.1016\/j.biopha.2026.119680_bib64","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/hup.561","article-title":"Sedation, an unpleasant, undesirable and potentially dangerous side-effect of many psychotropic drugs","volume":"19","author":"Bourin","year":"2004","journal-title":"Human. Psychopharmacol. Clin. Exp."},{"issue":"7","key":"10.1016\/j.biopha.2026.119680_bib65","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1002\/phar.2767","article-title":"Adverse effects of antidepressant medications and their management in children and adolescents","volume":"43","author":"Strawn","year":"2023","journal-title":"Pharmacotherapy"},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib66","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1515\/rns.2011.009","article-title":"Zebrafish assessment of cognitive improvement and anxiolysis: filling the gap between in vitro and rodent models for drug development","volume":"22","author":"Levin","year":"2011","journal-title":"Rev. Neurosci."},{"issue":"46","key":"10.1016\/j.biopha.2026.119680_bib67","doi-asserted-by":"crossref","first-page":"10682","DOI":"10.1523\/JNEUROSCI.1166-05.2005","article-title":"Evidence for a significant role of alpha 3-containing GABAA receptors in mediating the anxiolytic effects of benzodiazepines","volume":"25","author":"Dias","year":"2005","journal-title":"J. Neurosci. Off. J. Soc. Neurosci."},{"key":"10.1016\/j.biopha.2026.119680_bib68","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.neuropharm.2018.09.026","article-title":"TP003 is a non-selective benzodiazepine site agonist that induces anxiolysis via \u03b12GABAA receptors","volume":"143","author":"Neumann","year":"2018","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.biopha.2026.119680_bib69","unstructured":"Fischer, B.D., Atack, J.R., Platt, D.M., Reynolds, D.S., Dawson, G.R., & Rowlett, J.K. (n.d.). Behavioral pharmacology of the \u03b13 GABAA receptor positive modulator TP003 in monkeys. FASEB J., 25, 795.1-795.1. https:\/\/doi.org\/10.1096\/FASEBJ.25.1_SUPPLEMENT.795.1."},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib70","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1053\/j.gastro.2018.05.033","article-title":"GABAA receptor subtypes regulate stress-induced colon inflammation in mice","volume":"155","author":"Seifi","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.biopha.2026.119680_bib71","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2024.107356","article-title":"Pharmacological chaperones restore proteostasis of epilepsy-associated GABAA receptor variants","volume":"208","author":"Wang","year":"2024","journal-title":"Pharmacol. Res."},{"issue":"6","key":"10.1016\/j.biopha.2026.119680_bib72","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.1021\/acs.est.5b05665","article-title":"Changes in neurotransmitter profiles during early zebrafish (Danio rerio) development and after pesticide exposure","volume":"50","author":"Tufi","year":"2016","journal-title":"Environ. Sci. Technol."},{"issue":"8","key":"10.1016\/j.biopha.2026.119680_bib73","doi-asserted-by":"crossref","DOI":"10.1002\/jdn.10387","article-title":"GABA in early brain development: a dual role review","volume":"84","author":"Ji","year":"2024","journal-title":"Int. J. Dev. Neurosci."},{"issue":"7","key":"10.1016\/j.biopha.2026.119680_bib74","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103232","article-title":"Diazepam and fluoxetine decrease the stress response in zebrafish","volume":"9","author":"De Abreu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.biopha.2026.119680_bib75","article-title":"Effects of parental exposure to amitriptyline on the survival, development, behavior, and gene expression in zebrafish offspring","volume":"912","author":"Liu","year":"2024","journal-title":"Sci. Total. Environ."},{"issue":"4","key":"10.1016\/j.biopha.2026.119680_bib76","doi-asserted-by":"crossref","first-page":"738","DOI":"10.3390\/cells10040738","article-title":"Antidepressant screening demonstrated non-monotonic responses to amitriptyline, amoxapine and sertraline in locomotor activity assay in larval zebrafish","volume":"10","author":"Suryanto","year":"2021","journal-title":"Cells"},{"issue":"1\u20132","key":"10.1016\/j.biopha.2026.119680_bib77","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0166-4328(02)00311-X","article-title":"Anxiolytic-like effects of 5-HT2 ligands on three mouse models of anxiety","volume":"140","author":"Nic Dhonnchadha","year":"2003","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.biopha.2026.119680_bib78","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2019.107838","article-title":"The 5-hydroxytryptamine 2A receptor agonists DOI and 25CN-NBOH decrease marble burying and reverse 8-OH-DPAT-induced deficit in spontaneous alternation","volume":"183","author":"Odland","year":"2021","journal-title":"Neuropharmacology"},{"issue":"4","key":"10.1016\/j.biopha.2026.119680_bib79","first-page":"1046","article-title":"High-resolution tracking of unconfined zebrafish behavior reveals stimulatory and anxiolytic effects of psilocybin","volume":"29","author":"Braun","year":"2024","journal-title":"Mol. Psychiatry 2024 294"},{"key":"10.1016\/j.biopha.2026.119680_bib80","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.brainres.2012.02.022","article-title":"Distinct models of induced hyperactivity in zebrafish larvae","volume":"1449","author":"Ellis","year":"2012","journal-title":"Brain Res."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib81","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/jnc.15587","article-title":"The clinical pharmacology and potential therapeutic applications of 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT)","volume":"162","author":"Reckweg","year":"2022","journal-title":"J. Neurochem."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib82","doi-asserted-by":"crossref","DOI":"10.1038\/s41380-024-02655-w","article-title":"Serotonergic psychedelic 5-MeO-DMT alters plasticity-related gene expression and generates anxiolytic effects in stressed mice","volume":"30","author":"Nogueira","year":"2024","journal-title":"Mol. Psychiatry"},{"key":"10.1016\/j.biopha.2026.119680_bib83","doi-asserted-by":"crossref","first-page":"139","DOI":"10.3389\/fnbeh.2017.00139","article-title":"Behavioral changes over time following ayahuasca exposure in zebrafish","volume":"11","author":"Savoldi","year":"2017","journal-title":"Front. Behav. Neurosci."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib84","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12868-016-0298-z","article-title":"Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish","volume":"17","author":"Bai","year":"2016","journal-title":"BMC Neurosci."},{"issue":"10","key":"10.1016\/j.biopha.2026.119680_bib85","doi-asserted-by":"crossref","first-page":"173","DOI":"10.21037\/atm.2018.04.25","article-title":"Characterization of the locomotor activities of zebrafish larvae under the influence of various neuroactive drugs","volume":"6","author":"Li","year":"2018","journal-title":"Ann. Transl. Med."},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib86","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12868-016-0298-z","article-title":"Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish","volume":"17","author":"Bai","year":"2016","journal-title":"BMC Neurosci."},{"issue":"2","key":"10.1016\/j.biopha.2026.119680_bib87","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1097\/00004714-200304000-00014","article-title":"Antidepressive treatment with amitriptyline and paroxetine: effects on saliva cortisol concentrations","volume":"23","author":"Deuschle","year":"2003","journal-title":"J. Clin. Psychopharmacol."},{"issue":"9","key":"10.1016\/j.biopha.2026.119680_bib88","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1002\/cbin.12200","article-title":"Amitriptyline and cholecalciferol amend hippocampal histological structure and myelination during stress in Wistar rats via regulating miR200\/BMP4\/Olig-2 signaling","volume":"48","author":"Roushdy","year":"2024","journal-title":"Cell. Biol. Int."},{"issue":"7","key":"10.1016\/j.biopha.2026.119680_bib89","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103232","article-title":"Diazepam and fluoxetine decrease the stress response in zebrafish","volume":"9","author":"De Abreu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.biopha.2026.119680_bib90","doi-asserted-by":"crossref","DOI":"10.1016\/j.neulet.2023.137358","article-title":"Diazepam-Induced Down-Regulation of the GABAA receptor \u03b11 Subunit, as mediated by the activation of L-Type Voltage-Gated calcium Channel\/Ca2+\/Protein kinase a signaling cascade","volume":"810","author":"Gonz\u00e1lez G\u00f3mez","year":"2023","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.biopha.2026.119680_bib91","article-title":"Diazepam","author":"Islam","year":"2025","journal-title":"Mol. Neurobiol."},{"issue":"7\u20138","key":"10.1016\/j.biopha.2026.119680_bib92","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/dta.1333","article-title":"Head-twitch response in rodents induced by the hallucinogen 2,5-dimethoxy-4-iodoamphetamine: a comprehensive history, a re-evaluation of mechanisms, and its utility as a model","volume":"4","author":"Canal","year":"2012","journal-title":"Drug. Test. Anal."},{"issue":"7","key":"10.1016\/j.biopha.2026.119680_bib93","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1038\/s41386-022-01324-2","article-title":"Effects of a psychedelic 5-HT2A receptor agonist on anxiety-related behavior and fear processing in mice","volume":"47","author":"P\u0119dzich","year":"2022","journal-title":"Neuropsychopharmacology"},{"issue":"2","key":"10.1016\/j.biopha.2026.119680_bib94","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/S0028-3908(01)00166-6","article-title":"Comparison of the potency of MDL 100,907 and SB 242084 in blocking the serotonin (5-HT)2 receptor agonist-induced increases in rat serum corticosterone concentrations: Evidence for 5-HT2A receptor mediation of the HPA axis","volume":"42","author":"Hemrick-Luecke","year":"2002","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.biopha.2026.119680_bib95","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1210\/endo-127-4-1574","article-title":"Recovery of the rat hypothalamic-pituitary-adrenal axis after discontinuation of prolonged treatment with the synthetic glucocorticoid agonist dexamethasone","volume":"127","author":"Calogero","year":"1990","journal-title":"Endocrinology"},{"issue":"1","key":"10.1016\/j.biopha.2026.119680_bib96","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ygcen.2008.09.011","article-title":"The zebrafish stress axis: molecular fallout from the teleost-specific genome duplication event","volume":"161","author":"Alsop","year":"2009","journal-title":"General. Comp. Endocrinol."},{"issue":"34","key":"10.1016\/j.biopha.2026.119680_bib97","doi-asserted-by":"crossref","first-page":"34943","DOI":"10.1007\/s11356-019-06619-4","article-title":"Environmental concentration of fluoxetine disturbs larvae behavior and increases the defense response at molecular level in zebrafish (Danio rerio)","volume":"26","author":"Parolini","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"issue":"5","key":"10.1016\/j.biopha.2026.119680_bib98","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0063302","article-title":"Chronic Unpredictable Stress (CUS)-induced anxiety and related mood disorders in a zebrafish model: altered brain proteome profile implicates mitochondrial dysfunction","volume":"8","author":"Chakravarty","year":"2013","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.biopha.2026.119680_bib99","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1007\/s10072-022-05914-2","article-title":"Plasma neurofilament light chain levels are associated with depressive and anxiety symptoms in Parkinson\u2019s disease","volume":"43","author":"Yin","year":"2022","journal-title":"Neurol. Sci."},{"key":"10.1016\/j.biopha.2026.119680_bib100","first-page":"1","article-title":"Increased serum neurofilament light chain concentration associated with microglial morphology changes in chronically-starved mice","volume":"0","author":"Zimmermann","year":"2025","journal-title":"Int. J. Eat. Disord."},{"key":"10.1016\/j.biopha.2026.119680_bib101","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2023.109574","article-title":"Chronic exposure to environmentally relevant concentrations of carbamazepine interferes with anxiety response of adult female zebrafish through GABA \/5-HT pathway and HPI axis","volume":"266","author":"Xie","year":"2023","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"issue":"12","key":"10.1016\/j.biopha.2026.119680_bib102","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1080\/15592294.2018.1554520","article-title":"Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon","volume":"13","author":"Uren Webster","year":"2018","journal-title":"Epigenetics"},{"issue":"3","key":"10.1016\/j.biopha.2026.119680_bib103","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1530\/JOE-12-0379","article-title":"11\u03b2-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation","volume":"215","author":"Alderman","year":"2012","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.biopha.2026.119680_bib104","article-title":"Zebrafish as a model to investigate the CRH axis and interactions with DISC1","volume":"26","author":"Eachus","year":"2022","journal-title":"Curr. Opin. Endocr. Metab. Res."},{"key":"10.1016\/j.biopha.2026.119680_bib105","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2023.109574","article-title":"Chronic exposure to environmentally relevant concentrations of carbamazepine interferes with anxiety response of adult female zebrafish through GABA \/5-HT pathway and HPI axis","volume":"266","author":"Xie","year":"2023","journal-title":"Comp. Biochem. Physiol. Part - C Toxicol. Pharmacol."},{"key":"10.1016\/j.biopha.2026.119680_bib106","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2020.111014","article-title":"Tributyltin enhanced anxiety of adult male zebrafish through elevating cortisol level and disruption in serotonin, dopamine and gamma-aminobutyric acid neurotransmitter pathways","volume":"203","author":"Tu","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"Pt 24","key":"10.1016\/j.biopha.2026.119680_bib107","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1113\/jphysiol.2013.256826","article-title":"Physiological roles of glucocorticoids during early embryonic development of the zebrafish (Danio rerio)","volume":"591","author":"Wilson","year":"2013","journal-title":"J. Physiol."},{"key":"10.1016\/j.biopha.2026.119680_bib108","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1038\/sj.npp.1301076","article-title":"Effects of antidepressants on the brain\/plasma distribution of corticosterone","volume":"31","author":"Weber","year":"2006","journal-title":"Neuropsychopharmacology"},{"issue":"1\u20134","key":"10.1016\/j.biopha.2026.119680_bib109","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.aquaculture.2009.08.035","article-title":"Whole-body cortisol response of zebrafish to acute net handling stress","volume":"297","author":"Ramsay","year":"2009","journal-title":"Aquac. (Amst. Neth.)"},{"issue":"10","key":"10.1016\/j.biopha.2026.119680_bib110","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot5314","article-title":"Quantitative real-time RT-PCR (qRT-PCR) of zebrafish transcripts: Optimization of RNA extraction, quality control considerations, and data analysis","volume":"4","author":"Lan","year":"2009","journal-title":"Cold Spring Harb. Protoc."}],"container-title":["Biomedicine & Pharmacotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S075333222600716X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S075333222600716X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T19:50:37Z","timestamp":1789329037000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S075333222600716X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":110,"alternative-id":["S075333222600716X"],"URL":"https:\/\/doi.org\/10.1016\/j.biopha.2026.119680","relation":{},"ISSN":["0753-3322"],"issn-type":[{"value":"0753-3322","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Anxiety-related platform in zebrafish (Danio rerio) larvae: An integrated analysis of behavioural and molecular assay in drug screening research","name":"articletitle","label":"Article Title"},{"value":"Biomedicine & Pharmacotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biopha.2026.119680","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"119680"}}