{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:27:11Z","timestamp":1782210431341,"version":"3.54.5"},"reference-count":30,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2010,6]]},"DOI":"10.1016\/j.biortech.2010.01.117","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T10:36:53Z","timestamp":1267180613000},"page":"4261-4268","source":"Crossref","is-referenced-by-count":18,"title":["Immobilized aculeacin A acylase from Actinoplanes utahensis: Characterization of a novel biocatalyst"],"prefix":"10.1016","volume":"101","author":[{"given":"Daniel","family":"Hormigo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"de la Mata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Acebal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Arroyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2010.01.117_bib1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00253-002-1113-6","article-title":"Biotechnological applications of penicillin acylases: state-of-the-art","volume":"60","author":"Arroyo","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2010.01.117_bib2","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1002\/(SICI)1097-0290(19980605)58:5<486::AID-BIT4>3.0.CO;2-9","article-title":"A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports","volume":"58","author":"Bastida","year":"1998","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2010.01.117_bib3","doi-asserted-by":"crossref","first-page":"382","DOI":"10.7164\/antibiotics.42.382","article-title":"Deacylation of echinocandin B by Actinoplanes utahensis","volume":"42","author":"Boek","year":"1989","journal-title":"J. Antibiot."},{"key":"10.1016\/j.biortech.2010.01.117_bib4","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1021\/op015506w","article-title":"Eupergit oxirane acrylic beads: how to make enzymes fit for biocatalysis","volume":"6","author":"Boller","year":"2002","journal-title":"Org. Process Res. Dev."},{"key":"10.1016\/j.biortech.2010.01.117_bib5","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.biortech.2010.01.117_bib6","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/s002849900361","article-title":"Chainia penicillin V acylase: strain characteristics, enzyme immobilization, and kinetic studies","volume":"37","author":"Chauhan","year":"1998","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.biortech.2010.01.117_bib7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.7164\/antibiotics.42.389","article-title":"Synthesis of new analogues of echinocandin B by enzymatic deacylation and chemical reacylation of the echinocandin B peptide: synthesis of the antifungal agent cyclofungin (LY121019)","volume":"42","author":"Debono","year":"1989","journal-title":"J. Antibiot."},{"key":"10.1016\/j.biortech.2010.01.117_bib8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1021\/bm000072i","article-title":"Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions","volume":"2","author":"Fern\u00e1ndez-Lafuente","year":"2001","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.biortech.2010.01.117_bib9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.molcatb.2005.04.013","article-title":"Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides","volume":"35","author":"Ghazi","year":"2005","journal-title":"J. Mol. Catal. B: Enzym."},{"key":"10.1016\/j.biortech.2010.01.117_bib10","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1021\/bp0400083","article-title":"Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports","volume":"20","author":"G\u00f3mez de Segura","year":"2004","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.biortech.2010.01.117_bib11","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0141-0229(85)90013-4","article-title":"Categorization of enzyme deactivations using a series-type mechanism","volume":"7","author":"Henley","year":"1985","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.biortech.2010.01.117_bib12","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0141-0229(00)00150-2","article-title":"Immobilization\/stabilization on Eupergit C of the \u03b2-galactosidase from Bacillus circulans and an \u03b1-galactosidase from Aspergillus oryzae","volume":"27","author":"Hernaiz","year":"2000","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.biortech.2010.01.117_bib13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/10242420903051891","article-title":"Kinetic and microstructural characterization of immobilized penicillin acylase from Streptomyces lavendulae on Sepabeads EC-EP","volume":"27","author":"Hormigo","year":"2009","journal-title":"Biocatal. Biotransform."},{"key":"10.1016\/j.biortech.2010.01.117_bib14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jbiotec.2005.06.025","article-title":"Immobilization and biocatalysis of chlorophyllase in selected organic solvent systems","volume":"120","author":"Karboune","year":"2005","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.biortech.2010.01.117_bib15","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/0167-7799(93)90080-S","article-title":"Immobilized enzymes: learning from past successes and failures","volume":"11","author":"Katchalski-Katzir","year":"1993","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.biortech.2010.01.117_bib16","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S1381-1177(00)00124-7","article-title":"Eupergit C, a carrier for immobilization of enzymes of industrial potential","volume":"10","author":"Katchalski-Katzir","year":"2000","journal-title":"J. Mol. Catal. B: Enzym."},{"key":"10.1016\/j.biortech.2010.01.117_bib17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2010.01.117_bib18","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0141-0229(99)00188-X","article-title":"Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment","volume":"26","author":"Mateo","year":"2000","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.biortech.2010.01.117_bib19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1021\/bp010171n","article-title":"Epoxy Sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment","volume":"18","author":"Mateo","year":"2002","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.biortech.2010.01.117_bib20","doi-asserted-by":"crossref","first-page":"297","DOI":"10.7164\/antibiotics.30.297","article-title":"Studies on aculeacin. I. Isolation and characterization of aculeacin A","volume":"30","author":"Mizuno","year":"1977","journal-title":"J. Antibiot."},{"key":"10.1016\/j.biortech.2010.01.117_bib21","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1432-1033.1992.tb19840.x","article-title":"Immobilization and single-step purification of fusion proteins using DEAE-cellulose","volume":"198","author":"S\u00e1nchez-Puelles","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.biortech.2010.01.117_bib22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/BF00156291","article-title":"Evaluation of determination of 6-amino penicillanic acid by p-dimethylamino benzaldehyde","volume":"1","author":"Shewale","year":"1987","journal-title":"Biotechnol. Tech."},{"key":"10.1016\/j.biortech.2010.01.117_bib23","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2323\/jgam.34.333","article-title":"Deacylation of penicillins by the immobilized mycelia of the thermophile Malbranchea","volume":"34","author":"Singh","year":"1988","journal-title":"J. Gen. Appl. Microbiol."},{"key":"10.1016\/j.biortech.2010.01.117_bib24","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1007\/BF00369569","article-title":"Enzymatic splitting of penicillin V for the production of 6-APA using immobilized penicillin V acylase","volume":"9","author":"Sudhakaran","year":"1993","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2010.01.117_bib25","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1093\/oxfordjournals.jbchem.a122712","article-title":"A deacylation enzyme for aculeacin A, a neutral lipopeptide antibiotic, from Actinoplanes utahensis: purification and characterization","volume":"105","author":"Takeshima","year":"1989","journal-title":"J. Biochem."},{"key":"10.1016\/j.biortech.2010.01.117_bib26","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s002530051618","article-title":"Enhanced production of penicillin V acylase from Streptomyces lavendulae","volume":"53","author":"Torres","year":"1999","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2010.01.117_bib27","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1023\/A:1005601607277","article-title":"Covalent immobilization of penicillin acylase from Streptomyces lavendulae","volume":"22","author":"Torres-Bacete","year":"2000","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.biortech.2010.01.117_bib28","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1042\/BA20000042","article-title":"Optimization of 6-aminopenicillanic acid (6-APA) production by using a new immobilized penicillin acylase","volume":"32","author":"Torres-Bacete","year":"2000","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.biortech.2010.01.117_bib29","doi-asserted-by":"crossref","first-page":"5378","DOI":"10.1128\/AEM.00452-07","article-title":"Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis","volume":"73","author":"Torres-Bacete","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2010.01.117_bib30","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1006\/bbrc.2002.6485","article-title":"Substrate specificity of penicillin acylase from Streptomyces lavendulae","volume":"291","author":"Torres-Guzm\u00e1n","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852410002038?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852410002038?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,5,26]],"date-time":"2019-05-26T12:20:09Z","timestamp":1558873209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852410002038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["S0960852410002038"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2010.01.117","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}