{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:14:37Z","timestamp":1790439277450,"version":"4.1.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T00:00:00Z","timestamp":1472515200000},"content-version":"am","delay-in-days":303,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Research Foundation Flanders Belgium","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007000","name":"Laboratory Directed Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"BioEnergy Technology Office, U.S. Department of Energy","doi-asserted-by":"publisher","award":["9.1.1.3_DE-EE0005995"],"award-info":[{"award-number":["9.1.1.3_DE-EE0005995"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2015,11]]},"DOI":"10.1016\/j.biortech.2015.08.042","type":"journal-article","created":{"date-parts":[[2015,8,19]],"date-time":"2015-08-19T22:46:36Z","timestamp":1440024396000},"page":"656-661","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"special_numbering":"C","title":["Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite"],"prefix":"10.1016","volume":"196","author":[{"given":"Dries","family":"Vandamme","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip I.","family":"Pohl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelies","family":"Beuckels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imogen","family":"Foubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick V.","family":"Brady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Hewson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koenraad","family":"Muylaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2015.08.042_b0005","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/0043-1354(86)90157-0","article-title":"Seawater induced algal flocculation","volume":"20","author":"Ayoub","year":"1986","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0010","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1016\/j.rser.2014.09.037","article-title":"Harvesting techniques applied to microalgae: a review","volume":"41","author":"Barros","year":"2015","journal-title":"Renew. Sustain. Energy Rev."},{"key":"10.1016\/j.biortech.2015.08.042_b0015","series-title":"Algae Biomass","first-page":"457","article-title":"Development of microalgal harvesting and high-rate pond technologies in California","author":"Benemann","year":"1980"},{"key":"10.1016\/j.biortech.2015.08.042_b0020","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.biortech.2013.08.053","article-title":"High-pH-induced flocculation-flotation of the hypersaline microalga Dunaliella salina","volume":"147C","author":"Besson","year":"2013","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2015.08.042_b0025","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.biombioe.2013.03.027","article-title":"Influence of organic matter on flocculation of Chlorella vulgaris by calcium phosphate precipitation","volume":"54","author":"Beuckels","year":"2013","journal-title":"Biomass Bioenergy"},{"key":"10.1016\/j.biortech.2015.08.042_b0030","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.algal.2014.02.004","article-title":"A coordination chemistry model of algal autoflocculation","volume":"5","author":"Brady","year":"2014","journal-title":"Algal Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0035","series-title":"Coagulation and Flocculation in Water and Wastewater Treatment","author":"Bratby","year":"2006"},{"key":"10.1016\/j.biortech.2015.08.042_b0040","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.copbio.2012.09.012","article-title":"Food commodities from microalgae","volume":"24","author":"Draaisma","year":"2013","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.biortech.2015.08.042_b0045","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1016\/0043-1354(96)00142-X","article-title":"Suspended solids abatement by pH increase\u2014upgrading of an oxidation pond effluent","volume":"30","author":"Elmaleh","year":"1996","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0050","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1126\/science.1097329","article-title":"Impact of anthropogenic CO2 on the CaCO3 system in the oceans","volume":"305","author":"Feely","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.biortech.2015.08.042_b0055","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1039\/c1gc00015b","article-title":"Algae as a source of renewable chemicals: opportunities and challenges","volume":"13","author":"Foley","year":"2011","journal-title":"Green Chem."},{"key":"10.1016\/j.biortech.2015.08.042_b0060","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.algal.2013.11.016","article-title":"Influence of magnesium concentration, biomass concentration and pH on flocculation of Chlorella vulgaris","volume":"3","author":"Garc\u00eda-P\u00e9rez","year":"2014","journal-title":"Algal Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0065","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1002\/btpr.575","article-title":"Microalgae as sources of high added-value compounds \u2013 a brief review of recent work","volume":"27","author":"Guedes","year":"2011","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.biortech.2015.08.042_b0070","first-page":"1","article-title":"Yellow-green algae with chlorophyllide c","volume":"8","author":"Guillard","year":"1972","journal-title":"J. Phycol."},{"key":"10.1016\/j.biortech.2015.08.042_b0075","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1002\/bit.260300707","article-title":"Harvesting of scenedesmus obliquus in wastewaters: auto- or bioflocculation?","volume":"30","author":"Lavoie","year":"1987","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2015.08.042_b0080","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0734-9750(02)00050-2","article-title":"Recovery of microalgal biomass and metabolites: process options and economics","volume":"20","author":"Molina Grima","year":"2003","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.biortech.2015.08.042_b0085","unstructured":"Parkhurst, D.L., Appelo, A.J., 1999. User\u2019s Guide to PHREEQC (Version 2): A Computer Program for Speciation, Batch-reaction, One-dimensional Transport, and Inverse Geochemical Calculations."},{"key":"10.1016\/j.biortech.2015.08.042_b0090","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0016-7037(03)00238-2","article-title":"Experimental study of brucite dissolution and precipitation in aqueous solutions: surface speciation and chemical affinity control","volume":"68","author":"Pokrovsky","year":"2004","journal-title":"Geochim. Cosmochim. Acta"},{"key":"10.1016\/j.biortech.2015.08.042_b0095","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0304-3770(89)90050-8","article-title":"Bicarbonate utilization: function and mechanism","volume":"34","author":"Prins","year":"1989","journal-title":"Aquat. Bot."},{"key":"10.1016\/j.biortech.2015.08.042_b0100","article-title":"Use of natural pH variation to increase the flocculation of the marine microalgae Nannochloropsis oculata","author":"Sales","year":"2014","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.biortech.2015.08.042_b0105","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.biotechadv.2012.01.011","article-title":"Inexpensive non-toxic flocculation of microalgae contradicts theories; overcoming a major hurdle to bulk algal production","volume":"30","author":"Schlesinger","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.biortech.2015.08.042_b0110","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1016\/0016-7037(93)90002-E","article-title":"Calcite precipitation in seawater using a constant addition technique: a new overall reaction kinetic expression","volume":"57","author":"Shaojun","year":"1993","journal-title":"Geochim. Cosmochim. Acta"},{"key":"10.1016\/j.biortech.2015.08.042_b0115","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.biortech.2012.08.021","article-title":"Algal biomass dehydration","volume":"135","author":"Show","year":"2013","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2015.08.042_b0120","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.biortech.2013.01.037","article-title":"Potential pre-concentration methods for Nannochloropsis gaditana and a comparative study of pre-concentrated sample properties","volume":"132","author":"\u015eirin","year":"2013","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2015.08.042_b0125","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1007\/s10811-010-9616-5","article-title":"Inducing autoflocculation in the diatom Phaeodactylum tricornutum through CO2 regulation","volume":"23","author":"Spilling","year":"2011","journal-title":"J. Appl. Phycol."},{"key":"10.1016\/j.biortech.2015.08.042_b0130","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0043-1354(85)90333-1","article-title":"Coprecipitation of microalgal biomass with calcium and phosphate ions","volume":"19","author":"Sukenik","year":"1985","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0135","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/bit.260260206","article-title":"Algal autoflocculation\u2013verification and proposed mechanism","volume":"26","author":"Sukenik","year":"1984","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2015.08.042_b0140","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1007\/s12155-014-9554-1","article-title":"Reversible flocculation of microalgae using magnesium hydroxide","volume":"8","author":"Vandamme","year":"2015","journal-title":"BioEnergy Res."},{"key":"10.1016\/j.biortech.2015.08.042_b0145","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.biortech.2011.11.105","article-title":"Flocculation of Chlorella vulgaris induced by high pH: role of magnesium and calcium and practical implications","volume":"105","author":"Vandamme","year":"2012","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2015.08.042_b0150","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.tibtech.2012.12.005","article-title":"Flocculation as a low-cost method for harvesting microalgae for bulk biomass production","volume":"31","author":"Vandamme","year":"2013","journal-title":"Trends Biotechnol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852415011487?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852415011487?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T03:35:12Z","timestamp":1760758512000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852415011487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":30,"alternative-id":["S0960852415011487"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2015.08.042","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2015.08.042","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}