{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:32:22Z","timestamp":1790361142053,"version":"4.1.0"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2023,10]]},"DOI":"10.1016\/j.biortech.2023.129476","type":"journal-article","created":{"date-parts":[[2023,7,8]],"date-time":"2023-07-08T02:11:53Z","timestamp":1688782313000},"page":"129476","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Metalloenzymes play major roles to achieve high-rate nitrogen removal in N-damo communities: Lessons from metaproteomics"],"prefix":"10.1016","volume":"385","author":[{"given":"Silvana","family":"Quiton-Tapia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Trueba-Santiso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Suarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Omil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2023.129476_b0005","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2021.668910","article-title":"The Role of ClpB in Bacterial Stress Responses and Virulence","volume":"8","author":"Alam","year":"2021","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.biortech.2023.129476_b0010","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.cej.2018.04.134","article-title":"Enrichment of nitrite-dependent anaerobic methane oxidizing bacteria in a membrane bioreactor","volume":"347","author":"Allegue","year":"2018","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2023.129476_b0015","doi-asserted-by":"crossref","unstructured":"APHA, 2000. Standard methods for the examination of water and wastewater: 20th ed. https:\/\/doi.org\/10.5860\/choice.37-2792.","DOI":"10.5860\/CHOICE.37-2792"},{"key":"10.1016\/j.biortech.2023.129476_b0020","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jhazmat.2018.07.042","article-title":"An innovative wastewater treatment technology based on UASB and IFAS for cost-efficient macro and micropollutant removal","volume":"359","author":"Arias","year":"2018","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.biortech.2023.129476_b0025","doi-asserted-by":"crossref","unstructured":"Arias, A., Allegue, T., Darwich, A., Fachal-Suarez, M., Mart\u00ednez-quintela, M., Omil, F., Garrido, J.M., 2022. Operating strategies to optimize a membrane bioreactor enriched in nitrite-dependent anaerobic methane-oxidizing bacteria 450. https:\/\/doi.org\/10.1016\/j.cej.2022.138289.","DOI":"10.1016\/j.cej.2022.138289"},{"key":"10.1016\/j.biortech.2023.129476_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.ibiod.2020.104934","article-title":"Using proteomics for an insight into the performance of activated sludge in a lab-scale WWTP","volume":"149","author":"Azizan","year":"2020","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"10.1016\/j.biortech.2023.129476_b0035","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.csbj.2022.01.018","article-title":"Considerations for constructing a protein sequence database for metaproteomics","volume":"20","author":"Blakeley-Ruiz","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.biortech.2023.129476_b0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2020.121711","article-title":"Characterization of membrane-bound metalloproteins in the anaerobic ammonium-oxidizing bacterium \u201cCandidatus Kuenenia stuttgartiensis\u201d strain CSTR1","volume":"223","author":"Budhraja","year":"2021","journal-title":"Talanta"},{"key":"10.1016\/j.biortech.2023.129476_b0045","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.watres.2015.09.026","article-title":"Nitrate reduction by denitrifying anaerobic methane oxidizing microorganisms can reach a practically useful rate","volume":"87","author":"Cai","year":"2015","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2023.129476_b0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2020.110565","article-title":"Enhancement of nitrite reduction and enrichment of Methylomonas via conductive materials in a nitrite-dependent anaerobic methane oxidation system","volume":"193","author":"Chang","year":"2021","journal-title":"Environ. Res."},{"key":"10.1016\/j.biortech.2023.129476_b0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.159728","article-title":"Instrumental role of bioreactors in nitrate\/nitrite-dependent anaerobic methane oxidation-based biotechnologies for wastewater treatment: a review","volume":"857","author":"Chen","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2023.129476_b0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.155244","article-title":"Is the role of aerobic methanotrophs underestimated in methane oxidation under hypoxic conditions?","volume":"833","author":"Cheng","year":"2022","journal-title":"Science of The Total Environment"},{"key":"10.1016\/j.biortech.2023.129476_b0065","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1574-6968.2009.01747.x","article-title":"Twitching motility and biofilm formation are associated with tonB1 in Xylella fastidiosa","volume":"299","author":"Cursino","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.biortech.2023.129476_b0070","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1111\/j.1462-2920.2008.01724.x","article-title":"Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea","volume":"10","author":"Ettwig","year":"2008","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0075","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature08883","article-title":"Nitrite-driven anaerobic methane oxidation by oxygenic bacteria","volume":"464","author":"Ettwig","year":"2010","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.biortech.2023.129476_b0080","first-page":"273","article-title":"Bacterial oxygen production in the dark","volume":"7","author":"Ettwig","year":"2012","journal-title":"Front Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2021.766527","article-title":"The polygonal cell shape and surface protein layer of anaerobic methane-oxidizing methylomirabilis lanthanidiphila bacteria","volume":"12","author":"Gambelli","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2012.00061","article-title":"Trace metal requirements for microbial enzymes involved in the production and consumption of methane and nitrous oxide","volume":"3","author":"Glass","year":"2012","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0095","first-page":"2598","volume":"20","author":"Graf","year":"2018","journal-title":"Bloom of a denitrifying methanotroph, \u2018 Candidatus Methylomirabilis limnetica \u2019, in a deep stratified lake"},{"key":"10.1016\/j.biortech.2023.129476_b0100","series-title":"Key Physiology of a Nitrite-Dependent Methane-Oxidizing Enrichment Culture","first-page":"1","author":"Guerrero-Cruz","year":"2019"},{"key":"10.1016\/j.biortech.2023.129476_b0105","article-title":"Heterotrophic denitrification: An overlooked factor that contributes to nitrogen removal in n-DAMO mixed culture, Environmental Research, Volume 216","volume":"4","author":"Guo","year":"2023","journal-title":"Part"},{"key":"10.1016\/j.biortech.2023.129476_b0110","doi-asserted-by":"crossref","DOI":"10.1111\/1758-2229.12239","article-title":"In situ expression of nitrite-dependent anaerobic methane oxidation proteins by Candidatus Methylomirabilis oxyfera co-occurring with expressed anammox proteins in a contaminated aquifer","author":"Hanson","year":"2015","journal-title":"Microbiol. Rep Environ."},{"key":"10.1016\/j.biortech.2023.129476_b0115","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/nature12375","article-title":"Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage","volume":"500","author":"Haroon","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2023.129476_b0120","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.watres.2014.09.055","article-title":"The short- and long-term effects of environmental conditions on anaerobic methane oxidation coupled to nitrite reduction","volume":"68","author":"He","year":"2015","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2023.129476_b0125","first-page":"1","article-title":"A novel denitrifying methanotroph of the NC10 phylum and its microcolony","volume":"6","author":"He","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biortech.2023.129476_b0130","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.biortech.2018.07.139","article-title":"Effect of trace elements on the development of co-cultured nitrite-dependent anaerobic methane oxidation and methanogenic bacteria consortium","volume":"268","author":"Jiang","year":"2018","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0135","doi-asserted-by":"crossref","first-page":"4093","DOI":"10.1016\/j.watres.2009.03.001","article-title":"Nitrous oxide emission during wastewater treatment","volume":"43","author":"Kampschreur","year":"2009","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2023.129476_b0140","doi-asserted-by":"crossref","first-page":"6163","DOI":"10.1007\/s00253-014-5766-8","article-title":"PQQ-dependent methanol dehydrogenases: rare-earth elements make a difference","volume":"98","author":"Keltjens","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2023.129476_b0145","doi-asserted-by":"crossref","first-page":"13152","DOI":"10.1021\/acs.est.2c05001","article-title":"Sulfamethoxazole enhances specific enzymatic activities under aerobic heterotrophic conditions: a metaproteomic approach","volume":"56","author":"Kennes-Veiga","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0150","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.cels.2021.04.003","article-title":"Database-independent de novo metaproteomics of complex microbial communities","volume":"12","author":"Kleikamp","year":"2021","journal-title":"Cell Syst."},{"key":"10.1016\/j.biortech.2023.129476_b0155","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-01544-x","article-title":"Assessing species biomass contributions in microbial communities via metaproteomics","volume":"8","author":"Kleiner","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2023.129476_b0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.126847","article-title":"Physiology of anammox adaptation to low temperatures and promising biomarkers: A review","volume":"349","author":"Kouba","year":"2022","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2012.00185","article-title":"Complete genome of Ignavibacterium album, a metabolically versatile, flagellated, facultative anaerobe from the phylum Chlorobi","volume":"3","author":"Liu","year":"2012","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0170","doi-asserted-by":"crossref","first-page":"8981","DOI":"10.1021\/acs.est.7b01225","article-title":"Pathways and controls of N2O production in nitritation-anammox biomass","volume":"51","author":"Ma","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0175","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.freeradbiomed.2019.01.018","article-title":"Functional assessment of microbial superoxide dismutase isozymes suggests a differential role for each isozyme","volume":"134","author":"Najmuldeen","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.biortech.2023.129476_b0180","first-page":"0269","article-title":"How efficiently does a metabolically enhanced system with denitrifying anaerobic methane oxidizing microorganisms remove antibiotics?","volume":"122033","author":"Quiton-Tapia","year":"2023","journal-title":"Environmenta Pollution"},{"key":"10.1016\/j.biortech.2023.129476_b0185","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nature04617","article-title":"A microbial consortium couples anaerobic methane oxidation to denitrification","volume":"440","author":"Raghoebarsing","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2023.129476_b0190","article-title":"Metal enzymes in \u201cimpossible\u201d microorganisms catalyzing the anaerobic oxidation of ammonium and methane","author":"Reimann","year":"2015","journal-title":"Metal Ions Life Sci."},{"key":"10.1016\/j.biortech.2023.129476_b0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.122491","article-title":"New perspectives on microbial communities and biological nitrogen removal processes in wastewater treatment systems","volume":"297","author":"Ren","year":"2020","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0200","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1126\/science.aav2572","article-title":"Particulate methane monooxygenase contains only mononuclear copper centers","volume":"). 364","author":"Ross","year":"2019","journal-title":"Science (80-."},{"key":"10.1016\/j.biortech.2023.129476_b0205","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.tim.2020.01.006","article-title":"The long-term relationship between microbial metabolism and greenhouse gases","volume":"28","author":"Stein","year":"2020","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0210","first-page":"1","article-title":"Comparative genomics of Candidatus Methylomirabilis species and description of Ca","volume":"9","author":"Versantvoort","year":"2018","journal-title":"Methylomirabilis lanthanidiphila. Front. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0215","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.bbabio.2019.07.011","article-title":"Complexome analysis of the nitrite-dependent methanotroph Methylomirabilis lanthanidiphila","volume":"1860","author":"Versantvoort","year":"2019","journal-title":"Biochim. Biophys. Acta - Bioenerg."},{"key":"10.1016\/j.biortech.2023.129476_b0220","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.watres.2019.05.101","article-title":"Achieving high nitrogen removal efficiency by optimizing nitrite-dependent anaerobic methane oxidation process with growth factors","volume":"161","author":"Wang","year":"2019","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2023.129476_b0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2020.124231","article-title":"Stable nitrogen removal by anammox process after rapid temperature drops: Insights from metagenomics and metaproteomics","volume":"320","author":"Wang","year":"2021","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0230","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/j.scitotenv.2018.11.321","article-title":"Roles and correlations of functional bacteria and genes in the start-up of simultaneous anammox and denitrification system for enhanced nitrogen removal","volume":"655","author":"Wang","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2023.129476_b0235","doi-asserted-by":"crossref","first-page":"6940","DOI":"10.1021\/acs.est.6b00546","article-title":"Expression of the nirS, hzsA, and hdh Genes in Response to Nitrite Shock and Recovery in Candidatus Kuenenia stuttgartiensis","volume":"50","author":"Wang","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2023.129476_b0240","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.tim.2011.11.004","article-title":"MreB: Pilot or passenger of cell wall synthesis?","volume":"20","author":"White","year":"2012","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0245","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1128\/AEM.03292-14","article-title":"XoxF-type methanol dehydrogenase from the anaerobic methanotroph \u201cCandidatus Methylomirabilis oxyfera\u201d","volume":"81","author":"Wu","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2023.129476_b0250","article-title":"Protein analysis by shotgun\/bottom-up proteomics","volume":"1\u201338","author":"Zhang","year":"2013","journal-title":"Chem. Rev."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852423009045?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852423009045?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T05:01:43Z","timestamp":1760763703000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852423009045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":50,"alternative-id":["S0960852423009045"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2023.129476","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metalloenzymes play major roles to achieve high-rate nitrogen removal in N-damo communities: Lessons from metaproteomics","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2023.129476","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"129476"}}