{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T13:42:24Z","timestamp":1793367744069,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.biortech.2025.132809","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T08:11:16Z","timestamp":1750061476000},"page":"132809","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Coproduction of single cell protein and pyrroloquinoline quinone by Hyphomicrobium denitrificans using molasses and biogas slurry"],"prefix":"10.1016","volume":"434","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8143-3779","authenticated-orcid":false,"given":"Daoyang","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuya","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2025.132809_b0005","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.foodchem.2013.07.105","article-title":"Solid state fermentation of food waste mixtures for single cell protein, aroma volatiles and fat production","volume":"145","author":"Aggelopoulos","year":"2014","journal-title":"Food Chem."},{"key":"10.1016\/j.biortech.2025.132809_b0010","doi-asserted-by":"crossref","unstructured":"B, E. 1991. Comments on report of a joint FAO\/WHO expert consultation on protein quality evaluation, Rome 1990. Z Ernahrungswiss, 30(2).","DOI":"10.1007\/BF01610063"},{"key":"10.1016\/j.biortech.2025.132809_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.gloenvcha.2020.102056","article-title":"Meeting the food security challenge for nine billion people in 2050: what impact on forests?","volume":"62","author":"Bahar","year":"2020","journal-title":"Glob. Environ. Chang."},{"issue":"4","key":"10.1016\/j.biortech.2025.132809_b0020","doi-asserted-by":"crossref","DOI":"10.1007\/s10661-015-4377-x","article-title":"Contamination and risk assessment of heavy metals in soils irrigated with biogas slurry: a case study of taihu basin","volume":"187","author":"Bian","year":"2015","journal-title":"Environ. Monit. Assess."},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0030","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/jctb.503310107","article-title":"Single\u2010cell protein from methanol: economic aspects of the norprotein\u00ae process","volume":"31","author":"Ericsson","year":"1981","journal-title":"J. Chem. Technol. Biotechnol."},{"issue":"10","key":"10.1016\/j.biortech.2025.132809_b0035","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1007\/s13399-022-02546-6","article-title":"Two-phase nutrient recovery from livestock wastewaters combining novel membrane technologies","volume":"12","author":"Gonz\u00e1lez-Garc\u00eda","year":"2022","journal-title":"Biomass Convers. Biorefin."},{"key":"10.1016\/j.biortech.2025.132809_b0040","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.biortech.2018.01.032","article-title":"Simultaneous treatment and single cell protein production from agri-industrial wastewaters using purple phototrophic bacteria or microalgae \u2013 a comparison","volume":"254","author":"H\u00fclsen","year":"2018","journal-title":"Bioresource Technology"},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0045","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.tibtech.2014.11.005","article-title":"Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges","volume":"33","author":"Jin","year":"2015","journal-title":"Trends Biotechnol."},{"issue":"2","key":"10.1016\/j.biortech.2025.132809_b0055","article-title":"Recent advances in the production of single cell protein from renewable resources and applications","volume":"7","author":"Koukoumaki","year":"2024","journal-title":"Carbon Resour. Convers."},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0060","article-title":"Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale","volume":"22","author":"Jiao","year":"2023","journal-title":"Microb Cell Fact"},{"issue":"4","key":"10.1016\/j.biortech.2025.132809_b0065","doi-asserted-by":"crossref","DOI":"10.2527\/jas.2017.1440","article-title":"Chemical composition and amino acid digestibility of soybean meal produced in the United States, China, Argentina, Brazil, or India","volume":"95","author":"Lagos","year":"2017","journal-title":"J. Anim. Sci."},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0070","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/1751-7915.12128","article-title":"Microbial response to single\u2010cell protein production and brewery wastewater treatment","volume":"8","author":"Lee","year":"2014","journal-title":"J. Microbial. Biotechnol."},{"issue":"4","key":"10.1016\/j.biortech.2025.132809_b0075","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s00726-020-02833-4","article-title":"Composition of amino acids and related nitrogenous nutrients in feedstuffs for animal diets","volume":"52","author":"Li","year":"2020","journal-title":"Amino Acids"},{"key":"10.1016\/j.biortech.2025.132809_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2025.132252","article-title":"Production of single-cell protein from vinegar residue by Rhodotorula glutinis and techno-economic analysis","volume":"422","author":"Li","year":"2025","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.132809_b0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.130119","article-title":"Efficient production of single cell protein from biogas slurry using screened alkali-salt-tolerant Debaryomyces hansenii","volume":"393","author":"Liu","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"18","key":"10.1016\/j.biortech.2025.132809_b0100","doi-asserted-by":"crossref","DOI":"10.3390\/foods11182831","article-title":"Single-cell proteins obtained by Circular economy intended as a feed ingredient in aquaculture","volume":"11","author":"Pereira","year":"2022","journal-title":"Foods"},{"key":"10.1016\/j.biortech.2025.132809_b0105","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.cbpa.2018.09.019","article-title":"Role of rare earth elements in methanol oxidation","volume":"49","author":"Picone","year":"2019","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.biortech.2025.132809_b0110","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.chemosphere.2016.11.163","article-title":"Heavy metal contamination of freshwater prawn (Macrobrachium rosenbergii) and prawn feed in Bangladesh: a market-based study to highlight probable health risks","volume":"170","author":"Rabiul Islam","year":"2017","journal-title":"Chemosphere"},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0115","doi-asserted-by":"crossref","DOI":"10.1186\/s13068-023-02261-y","article-title":"Adaptive evolutionary strategy coupled with an optimized biosynthesis process for the efficient production of pyrroloquinoline quinone from methanol","volume":"16","author":"Ren","year":"2023","journal-title":"Biotechnology for Biofuels and Bioproducts"},{"key":"10.1016\/j.biortech.2025.132809_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2023.739861","article-title":"Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles","volume":"576","author":"Ruiz","year":"2023","journal-title":"Aquaculture"},{"key":"10.1016\/j.biortech.2025.132809_b0125","first-page":"20","article-title":"Dietary pyrroloquinoline quinone improvement of the antioxidant capacity of laying hens and eggs are linked to the alteration of Nrf2\/HO-1 pathway and gut microbiota","volume":"X","author":"Shao","year":"2023","journal-title":"Food Chem."},{"key":"10.1016\/j.biortech.2025.132809_b0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2020.735885","article-title":"Single cell protein: sources, mechanism of production, nutritional value and its uses in aquaculture nutrition","volume":"531","author":"Sharif","year":"2021","journal-title":"Aquaculture"},{"key":"10.1016\/j.biortech.2025.132809_b0135","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.aninu.2023.06.015","article-title":"Dietary supplementation with pyrroloquinoline quinone promotes growth, relieves weaning stress, and regulates metabolism of piglets compared with adding zinc oxide","volume":"15","author":"Shi","year":"2023","journal-title":"Anim. Nutr."},{"issue":"12","key":"10.1016\/j.biortech.2025.132809_b0140","doi-asserted-by":"crossref","first-page":"4915","DOI":"10.1007\/s00253-017-8259-8","article-title":"Enhanced fed-batch production of pyrroloquinoline quinine in methylobacillus sp. CCTCC M2016079 with a two-stage pH control strategy","volume":"101","author":"Si","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2025.132809_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.130242","article-title":"Production of single cell protein from brewer\u2019s spent grain through enzymatic saccharification and fermentation enhanced by ammoniation pretreatment","volume":"394","author":"Sun","year":"2024","journal-title":"Bioresource Technology"},{"issue":"5","key":"10.1016\/j.biortech.2025.132809_b0155","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1039\/D2FO01515C","article-title":"Pyrroloquinoline quinone disodium salt improves brain function in both younger and older adults","volume":"14","author":"Tamakoshi","year":"2023","journal-title":"Food Funct."},{"issue":"1","key":"10.1016\/j.biortech.2025.132809_b0160","doi-asserted-by":"crossref","DOI":"10.1007\/s11033-025-10491-6","article-title":"Current study on pyrroloquinoline quinone (PQQ) therapeutic role in neurodegenerative diseases","volume":"52","author":"Xie","year":"2025","journal-title":"Mol Biol Rep"},{"issue":"11","key":"10.1016\/j.biortech.2025.132809_b0165","article-title":"Safety of pyrroloquinoline quinone disodium salt as a novel food pursuant to regulation (EC)No258\/97","volume":"15","author":"Turck","year":"2017","journal-title":"EFSA J."},{"issue":"12","key":"10.1016\/j.biortech.2025.132809_b0170","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.4014\/jmb.0907.07027","article-title":"High-cell-density fed-batch culture of Saccharomyces cerevisiae KV-25 using molasses and corn steep liquor","volume":"19","author":"Vu","year":"2009","journal-title":"J. Microbiol. Biotechnol."},{"issue":"4","key":"10.1016\/j.biortech.2025.132809_b0175","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1007\/s11157-022-09635-y","article-title":"Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment","volume":"21","author":"Wada","year":"2022","journal-title":"Rev. Environ. Sci. Bio\/technol."},{"issue":"6","key":"10.1016\/j.biortech.2025.132809_b0180","doi-asserted-by":"crossref","first-page":"e410","DOI":"10.1016\/S2542-5196(24)00064-0","article-title":"Investigating intervention components and their effectiveness in promoting environmentally sustainable diets: a systematic review","volume":"8","author":"Wadi","year":"2024","journal-title":"The Lancet Planetary Health"},{"key":"10.1016\/j.biortech.2025.132809_b0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2025.132397","article-title":"Single cell protein production potential of enriched microbial populations from rice paddy soils and roots: insights into protein yield enhancement by methylophilaceae","volume":"427","author":"Xu","year":"2025","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.biortech.2025.132809_b0190","article-title":"Production of single cell protein rich in potassium by Nectaromyces rattus using biogas slurry and molasses","volume":"350","author":"Yi","year":"2023","journal-title":"J Environ Manage"},{"key":"10.1016\/j.biortech.2025.132809_b0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.anifeedsci.2022.115556","article-title":"Substituting fish meal with a bacteria protein (methylococcus capsulatus, Bath) grown on natural gas: effects on growth non-specific immunity and gut health of spotted seabass (Lateolabrax maculatus)","volume":"296","author":"Yu","year":"2023","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.1016\/j.biortech.2025.132809_b0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.jclepro.2022.133279","article-title":"Upcycling waste organic acids and nitrogen into single cell protein via brewer's yeast","volume":"369","author":"Zeng","year":"2022","journal-title":"J. Clean. Prod."},{"key":"10.1016\/j.biortech.2025.132809_b0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.129345","article-title":"Coproduction of single cell protein and lipid from lignocellulose derived carbohydrates and inorganic ammonia salt with soluble ammonia recycling","volume":"384","author":"Zhang","year":"2023","journal-title":"Bioresour. Technol."},{"issue":"9","key":"10.1016\/j.biortech.2025.132809_b0210","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101334","article-title":"The production of single cell protein from biogas slurry with high ammonia-nitrogen content by screened Nectaromyces rattus","volume":"100","author":"Zhang","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.biortech.2025.132809_b0215","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131797","article-title":"Characterization of cotton stalk as a lignocellulosic feedstock for single-cell protein production","volume":"417","author":"Zhong","year":"2025","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.132809_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2024.109627","article-title":"Fluorescence-activated cell sorting and random mutagenesis for enhanced pyrroloquinoline quinone accumulation by hyphomicrobium denitrificans","volume":"215","author":"Zhu","year":"2025","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.biortech.2025.132809_b0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpa.2020.05.001","article-title":"Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone","volume":"59","author":"Zhu","year":"2020","journal-title":"Curr Opin Chem Biol"}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425007758?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425007758?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:29:35Z","timestamp":1761395375000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852425007758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":40,"alternative-id":["S0960852425007758"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.132809","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Coproduction of single cell protein and pyrroloquinoline quinone by Hyphomicrobium denitrificans using molasses and biogas slurry","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.132809","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"132809"}}