{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:03:31Z","timestamp":1787583811607,"version":"build-2736575974"},"reference-count":56,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100021193","name":"Xinjiang Production and Construction Corps Xinjiang Production and Construction Corps","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021193","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.biortech.2025.132967","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T12:09:58Z","timestamp":1752581398000},"page":"132967","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Constructing CRISPR-Cas9 system for metabolic reprogramming and cordycepin biomanufacturing in Pichia pastoris"],"prefix":"10.1016","volume":"436","author":[{"given":"Bingjie","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuquan","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0134-2849","authenticated-orcid":false,"given":"Yanbin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.biortech.2025.132967_b0005","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S1097-2765(03)00062-5","article-title":"Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery","volume":"11","author":"Agne","year":"2003","journal-title":"Mol. Cell"},{"key":"10.1016\/j.biortech.2025.132967_b0010","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.biocel.2018.09.019","article-title":"Yeast peroxisomes: how are they formed and how do they grow?","volume":"105","author":"Ak\u015fit","year":"2018","journal-title":"Int. J. Biochem. Cell. b."},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0015","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.synbio.2023.08.007","article-title":"The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing","volume":"8","author":"Bai","year":"2023","journal-title":"Syn. Syst. Biotechno."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0020","doi-asserted-by":"crossref","DOI":"10.1093\/femsre\/fux053","article-title":"Environmental factors influencing the development and spread of antibiotic resistance","volume":"42","author":"Bengtsson-Palme","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.biortech.2025.132967_b0025","doi-asserted-by":"crossref","first-page":"S523","DOI":"10.1016\/j.annonc.2024.08.732","article-title":"666P final results of phase I\/II study of NUC-7738 as monotherapy and in combination with pembrolizumab: Anti-tumor immune response in PD-1 inhibitor-resistant patients","volume":"35","author":"Blagden","year":"2024","journal-title":"Ann. Oncol."},{"issue":"13","key":"10.1016\/j.biortech.2025.132967_b0030","doi-asserted-by":"crossref","first-page":"7791","DOI":"10.1093\/nar\/gkab535","article-title":"Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichiapastoris","volume":"49","author":"Cai","year":"2021","journal-title":"Nucleic Acids Res."},{"issue":"29","key":"10.1016\/j.biortech.2025.132967_b0035","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2201711119","article-title":"Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris","volume":"119","author":"Cai","year":"2022","journal-title":"P. Natl. Acad. Sci. USA"},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/acssynbio.4c00530","article-title":"Microbial synthesis of nucleosides: advances and prospects","volume":"14","author":"Dai","year":"2024","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0045","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1021\/acssynbio.9b00372","article-title":"Host-informed expression of CRISPR guide RNA for genomic engineering in Komagataella phaffii","volume":"9","author":"Dalvie","year":"2019","journal-title":"ACS Synth. Biol."},{"issue":"6","key":"10.1016\/j.biortech.2025.132967_b0050","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/nsmb1251","article-title":"Interaction with the BRCA2 C terminus protects RAD51\u2013DNA filaments from disassembly by BRC repeats","volume":"14","author":"Davies","year":"2007","journal-title":"Nat. Struct. Mol. Biol."},{"issue":"11","key":"10.1016\/j.biortech.2025.132967_b0055","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1021\/acssynbio.3c00494","article-title":"Heterologous single-strand DNA-annealing and binding protein enhance CRISPR-based genome editing efficiency in Komagataella phaffii","volume":"12","author":"Deng","year":"2023","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2025.132967_b0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.127862","article-title":"High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica","volume":"363","author":"Duan","year":"2022","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.132967_b0065","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bej.2011.09.014","article-title":"Enhancement of cordycepin production in submerged cultures of Cordyceps militaris by addition of ferrous sulfate","volume":"60","author":"Fan","year":"2012","journal-title":"Biochem. Eng. J."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0070","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/acssynbio.1c00307","article-title":"Synthetic biology toolkit for marker-less integration of multigene pathways into Pichia pastoris via CRISPR\/Cas9","volume":"11","author":"Gao","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2025.132967_b0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.tibtech.2025.04.018","article-title":"Trends in sustainable single-cell protein from non-grain feedstocks","author":"Gao","year":"2025","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.biortech.2025.132967_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2025.108593","article-title":"Technological advancement spurs Komagataella phaffii as a next-generation platform for sustainable biomanufacturing","volume":"82","author":"Gao","year":"2025","journal-title":"Biotechnol. Adv."},{"issue":"8","key":"10.1016\/j.biortech.2025.132967_b0085","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202400261","article-title":"Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol","volume":"19","author":"Gao","year":"2024","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.biortech.2025.132967_b0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2024.05.002","article-title":"Engineering yeasts to Co-utilize methanol or formate coupled with CO2 fixation","volume":"84","author":"Guo","year":"2024","journal-title":"Metab. Eng."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0095","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1007\/s12011-018-1386-y","article-title":"Selenium biofortification and antioxidant activity in Cordyceps militaris supplied with selenate, selenite, or selenomethionine","volume":"187","author":"Hu","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0100","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-023-02261-5","article-title":"Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform","volume":"22","author":"Jeennor","year":"2023","journal-title":"Microb. Cell Fact."},{"issue":"11","key":"10.1016\/j.biortech.2025.132967_b0105","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.tibs.2015.09.001","article-title":"Chemistry and biology of self-cleaving ribozymes","volume":"40","author":"Jimenez","year":"2015","journal-title":"Trends Biochem. Sci"},{"key":"10.1016\/j.biortech.2025.132967_b0110","article-title":"Enhanced production of cordycepin in Ophiocordyceps sinensis using growth supplements under submerged conditions","volume":"28","author":"Kaushik","year":"2020","journal-title":"Biotechnol. Rep,"},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0115","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1007\/s00253-019-09623-3","article-title":"Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies","volume":"103","author":"Kunhorm","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2025.132967_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2024.103971","article-title":"Silkworm pupae globulin promotes Cordyceps militaris fermentation: Regulation of metabolic pathways enhances cordycepin synthesis and extends the synthesis phase","volume":"59","author":"Li","year":"2024","journal-title":"Food Biosci."},{"issue":"3","key":"10.1016\/j.biortech.2025.132967_b0125","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/acssynbio.9b00396","article-title":"Highly efficient biosynthesis of hypoxanthine in Escherichia coli and transcriptome-based analysis of the purine metabolism","volume":"9","author":"Liu","year":"2020","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0130","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-019-1194-x","article-title":"CRISPR\u2013Cas9-mediated genomic multiloci integration in Pichia pastoris","volume":"18","author":"Liu","year":"2019","journal-title":"Microb. Cell Fact."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0135","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1080\/07388551.2018.1554620","article-title":"Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production","volume":"39","author":"Liu","year":"2019","journal-title":"Crit. Rev. Biotechnol."},{"key":"10.1016\/j.biortech.2025.132967_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.129742","article-title":"High-level production of cordycepin by the xylose-utilising Cordyceps militaris strain 147 in an optimised medium","volume":"388","author":"Long","year":"2023","journal-title":"Bioresour. Technol."},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0145","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1021\/acs.jafc.2c08228","article-title":"Overproduction of patchoulol in metabolically engineered Komagataella phaffii","volume":"71","author":"Luo","year":"2023","journal-title":"J. Agric. Food Chem."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0150","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1093\/nar\/gkw1125","article-title":"Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament","volume":"45","author":"Ma","year":"2016","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0155","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-19842-2","article-title":"MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits","volume":"11","author":"Ma","year":"2020","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0160","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jbiosc.2010.08.018","article-title":"Production of cordycepin by a repeated batch culture of a Cordyceps militaris mutant obtained by proton beam irradiation","volume":"111","author":"Masuda","year":"2011","journal-title":"J. Biosci. Bioeng."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0165","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/s12934-023-02198-9","article-title":"Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale","volume":"22","author":"Meng","year":"2023","journal-title":"Microb. Cell Fact."},{"issue":"5","key":"10.1016\/j.biortech.2025.132967_b0170","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1021\/acssynbio.2c00591","article-title":"CMI: CRISPR\/Cas9 based efficient multiplexed integration in Saccharomyces cerevisiae","volume":"12","author":"Meng","year":"2023","journal-title":"ACS Synth. Biol."},{"issue":"6\u20137","key":"10.1016\/j.biortech.2025.132967_b0175","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.enzmictec.2011.02.004","article-title":"Biochemical characterisation of a NADPH-dependent carbonyl reductase from Neurospora crassa reducing \u03b1- and \u03b2-keto esters","volume":"48","author":"Richter","year":"2011","journal-title":"Enzyme Microb Tech."},{"issue":"14","key":"10.1016\/j.biortech.2025.132967_b0180","doi-asserted-by":"crossref","first-page":"7854","DOI":"10.1021\/acs.chemrev.6b00174","article-title":"Recent trends in nucleotide synthesis","volume":"116","author":"Roy","year":"2016","journal-title":"Chem. Rev."},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0185","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.synbio.2024.06.002","article-title":"Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris","volume":"9","author":"Ruan","year":"2024","journal-title":"Syn. Syst. Biotechno."},{"issue":"23","key":"10.1016\/j.biortech.2025.132967_b0190","doi-asserted-by":"crossref","first-page":"6500","DOI":"10.1158\/1078-0432.CCR-21-1652","article-title":"The novel nucleoside analogue protide NUC-7738 overcomes cancer resistance mechanisms in vitro and in a first-in-human phase I clinical trial","volume":"27","author":"Schwenzer","year":"2021","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.biortech.2025.132967_b0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131396","article-title":"Engineering high production of fatty alcohols from methanol by constructing coordinated dual biosynthetic pathways","volume":"412","author":"Shen","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"3","key":"10.1016\/j.biortech.2025.132967_b0200","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1021\/acssynbio.2c00570","article-title":"Increased cordycepin production in Yarrowia lipolytica using combinatorial metabolic engineering strategies","volume":"12","author":"Song","year":"2023","journal-title":"ACS Synth. Biol."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0205","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.synbio.2023.03.003","article-title":"Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level","volume":"8","author":"Tan","year":"2023","journal-title":"Syn. Syst. Biotechno."},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0210","doi-asserted-by":"crossref","DOI":"10.1002\/aic.18361","article-title":"Overproduction of cordycepin in Saccharomyces cerevisiae by cordycepin synthase screening and metabolic engineering","volume":"70","author":"Wang","year":"2024","journal-title":"AIChE J"},{"issue":"3","key":"10.1016\/j.biortech.2025.132967_b0215","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.synbio.2023.06.003","article-title":"A novel CRISPR\/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris","volume":"8","author":"Wang","year":"2023","journal-title":"Syn. Syst. Biotechno."},{"key":"10.1016\/j.biortech.2025.132967_b0225","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jbiotec.2016.03.027","article-title":"Combinatorial optimization of CRISPR\/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris","volume":"235","author":"Weninger","year":"2016","journal-title":"J. Biotechnol."},{"issue":"7","key":"10.1016\/j.biortech.2025.132967_b0230","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202400115","article-title":"Efficient CRISPR\u2010mediated C\u2010to\u2010T base editing in Komagataella phaffii","volume":"19","author":"Wu","year":"2024","journal-title":"Biotechnol. J."},{"issue":"16","key":"10.1016\/j.biortech.2025.132967_b0235","doi-asserted-by":"crossref","first-page":"6445","DOI":"10.1021\/acssuschemeng.3c00410","article-title":"Efficient bioproduction of 3-hydroxypropionic acid from methanol by a synthetic yeast cell factory","volume":"11","author":"Wu","year":"2023","journal-title":"ACS Sustain. Chem. Eng."},{"issue":"12","key":"10.1016\/j.biortech.2025.132967_b0240","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.chembiol.2017.09.001","article-title":"Fungal cordycepin biosynthesis is coupled with the production of the safeguard molecule pentostatin","volume":"24","author":"Xia","year":"2017","journal-title":"Cell Chem. Biol."},{"issue":"11","key":"10.1016\/j.biortech.2025.132967_b0245","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1073\/pnas.1420294112","article-title":"Boosting CRISPR\/Cas9 multiplex editing capability with the endogenous tRNA-processing system","volume":"112","author":"Xie","year":"2015","journal-title":"P. Natl. Acad. Sci. USA"},{"issue":"9","key":"10.1016\/j.biortech.2025.132967_b0250","doi-asserted-by":"crossref","first-page":"6999","DOI":"10.1074\/jbc.M110.202226","article-title":"NADPH-dependent reductases involved in the detoxification of reactive carbonyls in plants","volume":"286","author":"Yamauchi","year":"2011","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.biortech.2025.132967_b0255","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/yea.3001","article-title":"Hygromycin-resistance vectors for gene expression in Pichia pastoris","volume":"31","author":"Yang","year":"2014","journal-title":"Yeast"},{"key":"10.1016\/j.biortech.2025.132967_b0260","doi-asserted-by":"crossref","DOI":"10.1016\/j.fgb.2020.103431","article-title":"Synthesis of cordycepin: current scenario and future perspectives","volume":"143","author":"Yang","year":"2020","journal-title":"Fungal Genet. Biol."},{"issue":"2","key":"10.1016\/j.biortech.2025.132967_b0270","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1021\/acssynbio.4c00853","article-title":"De novo synthesis of reticuline and taxifolin using re-engineered homologous recombination in Yarrowia lipolytica","volume":"14","author":"Zhang","year":"2025","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2025.132967_b0275","article-title":"A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2025.132967_b0280","doi-asserted-by":"crossref","DOI":"10.1016\/j.lwt.2022.114354","article-title":"Synergistic effects of the Se and Zn supplemental combination on the nutrient improvement of mannitol and adenosine and the multi-element bioaccessibility in Cordyceps cicadae","volume":"173","author":"Zhao","year":"2023","journal-title":"LWT Food Sci. Technol."},{"key":"10.1016\/j.biortech.2025.132967_b0285","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131446","article-title":"Low-carbon and overproduction of cordycepin from methanol using engineered Pichia pastoris cell factory","volume":"413","author":"Zhao","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"24","key":"10.1016\/j.biortech.2025.132967_b0290","doi-asserted-by":"crossref","first-page":"7403","DOI":"10.1007\/s00253-023-12792-x","article-title":"Multifactorial interaction of selenium, iron, xylose, and glycine on cordycepin metabolism in Cordyceps militaris","volume":"107","author":"Zhao","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425009332?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425009332?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T12:04:08Z","timestamp":1762344248000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852425009332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":56,"alternative-id":["S0960852425009332"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.132967","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Constructing CRISPR-Cas9 system for metabolic reprogramming and cordycepin biomanufacturing in Pichia pastoris","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.132967","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"132967"}}