{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T01:43:44Z","timestamp":1780969424450,"version":"3.54.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon Europe","doi-asserted-by":"publisher","award":["955740"],"award-info":[{"award-number":["955740"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.biortech.2025.133407","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:36:15Z","timestamp":1759991775000},"page":"133407","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Aerobic autotrophic isopropanol production under low H2 atmosphere in Hollow Fiber Membrane Bioreactor"],"prefix":"10.1016","volume":"440","author":[{"given":"F.Di","family":"Bisceglie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Lombard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kratzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Gorret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Lorain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.E.","family":"Guillouet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2025.133407_b0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2024.109239","article-title":"Fermentation strategies for PHB production in a novel membrane bioreactor: Investigating batch and fed-batch operations","volume":"204","author":"Akkoyunlu","year":"2024","journal-title":"Biochem Eng J"},{"key":"10.1016\/j.biortech.2025.133407_b0010","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.apenergy.2016.12.151","article-title":"A novel gas separation integrated membrane bioreactor to evaluate the impact of self-generated biogas recycling on continuous hydrogen fermentation","volume":"190","author":"Bakonyi","year":"2017","journal-title":"Appl Energy"},{"issue":"6","key":"10.1016\/j.biortech.2025.133407_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2020.101218","article-title":"A Sustainable Chemicals Manufacturing Paradigm Using CO2 and Renewable H2","volume":"23","author":"Bommareddy","year":"2020","journal-title":"IScience"},{"key":"10.1016\/j.biortech.2025.133407_b0020","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jbiotec.2023.02.011","article-title":"Comparison of plasmid stabilization systems during heterologous isopropanol production in fed-batch bioreactor","volume":"366","author":"Boy","year":"2023","journal-title":"J Biotechnol"},{"issue":"9","key":"10.1016\/j.biortech.2025.133407_b0025","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1128\/AEM.02429-10","article-title":"Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains","volume":"77","author":"Budde","year":"2011","journal-title":"Appl. Env. Microbiol."},{"issue":"10","key":"10.1016\/j.biortech.2025.133407_b0030","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1002\/bit.26805","article-title":"Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin\u2013ferredoxin reductase systems","volume":"115","author":"Cr\u00e9pin","year":"2018","journal-title":"BiotechnoLl. Bioeng."},{"issue":"7718","key":"10.1016\/j.biortech.2025.133407_b0035","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/s41586-018-0383-9","article-title":"Marine heatwaves under global warming","volume":"560","author":"Fr\u00f6licher","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2025.133407_b0040","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.nbt.2019.11.005","article-title":"Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor","volume":"56","author":"Garrigues","year":"2020","journal-title":"New Biotechnol"},{"issue":"9","key":"10.1016\/j.biortech.2025.133407_b0045","doi-asserted-by":"crossref","first-page":"4277","DOI":"10.1007\/s00253-014-5591-0","article-title":"Isopropanol production with engineered Cupriavidus necator as bioproduction platform","volume":"98","author":"Grousseau","year":"2014","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.biortech.2025.133407_b0050","doi-asserted-by":"crossref","unstructured":"Hu, Q. , Zhang, X., Hao, H. 2023. A review of hydrogen-air cloud explosions:The fundamentals, overpressure prediction methods, and influencing factor. Intl J Hydrogen Energy 48, 13705-1373. https:\/\/doi.org\/10.1016\/j.ijhydene.2022.11.302.","DOI":"10.1016\/j.ijhydene.2022.11.302"},{"issue":"4","key":"10.1016\/j.biortech.2025.133407_b0055","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/0922-338X(91)90277-N","article-title":"Production of poly-\u03b2-hydroxybutyric acid from carbon dioxide by Alcaligenes eutrophus ATCC 17697T","volume":"71","author":"Ishizaki","year":"1991","journal-title":"J. Ferm. Bioeng."},{"issue":"1\u20132","key":"10.1016\/j.biortech.2025.133407_b0060","first-page":"6","article-title":"Microbial production of poly-D-3-hydroxybutyrate from CO2","volume":"57","author":"Ishizaki","year":"2001","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.biortech.2025.133407_b0065","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jbiotec.2024.04.009","article-title":"Impact of the environmental parameters on single cell protein production and composition by Cupriavidus necator","volume":"388","author":"Ismail","year":"2024","journal-title":"J Biotechnol"},{"issue":"5","key":"10.1016\/j.biortech.2025.133407_b0070","first-page":"1","article-title":"Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate","volume":"9","author":"Lambauer","year":"2022","journal-title":"Bioeng."},{"key":"10.1016\/j.biortech.2025.133407_b0075","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/0922-338X(93)90037-9","article-title":"Production of poly-\u03b2-hydroxybutyrate and measurement of related enzyme activities in Alcaligenes eutrophus","volume":"76","author":"Lee","year":"1993","journal-title":"J Ferment Bioeng"},{"key":"10.1016\/j.biortech.2025.133407_b0080","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2016.00694","article-title":"Gas Fermentation-A flexible platform for commercial scale production of low-carbon-fuels and chemicals from waste and renewable feedstocks","volume":"7","author":"Liew","year":"2016","journal-title":"Front Microbiol"},{"issue":"6290","key":"10.1016\/j.biortech.2025.133407_b9040","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1126\/science.aaf5039","article-title":"Water splitting-biosynthetic system with CO2 reduction efficiencies exceeding photosynthesis","volume":"352","author":"Liu","year":"2016","journal-title":"Science."},{"key":"10.1016\/j.biortech.2025.133407_b9005","series-title":"Isopropyl Alcohol","author":"Logsdon","year":"2000"},{"issue":"2","key":"10.1016\/j.biortech.2025.133407_b0090","first-page":"439","article-title":"Growth characteristics and cell composition of Alcaligenes eutrophus in chemostat culture","volume":"42","author":"Morinaga","year":"1978","journal-title":"Agric Biol Chem"},{"key":"10.1016\/j.biortech.2025.133407_b0095","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.biortech.2013.01.124","article-title":"A comparison of mass transfer coefficients between trickle-bed, Hollow fiber membrane and stirred tank reactors","volume":"133","author":"Orgill","year":"2013","journal-title":"Biores. Technol."},{"issue":"4","key":"10.1016\/j.biortech.2025.133407_b0100","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.tibtech.2021.01.001","article-title":"Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2","volume":"39","author":"Panich","year":"2021","journal-title":"Trends Biotechnol"},{"issue":"1","key":"10.1016\/j.biortech.2025.133407_b0105","first-page":"1","article-title":"Third-generation feed stocks for the clean and sustainable biotechnological production of bulk chemicals: Synthesis of 2-hydroxyisobutyric acid","volume":"2","author":"Przybylski","year":"2012","journal-title":"Energy Sustain. Soc."},{"issue":"5","key":"10.1016\/j.biortech.2025.133407_b0110","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1007\/s00253-014-6266-6","article-title":"Exploiting mixtures of H2, CO2, and O2 for improved production of methacrylate precursor 2-hydroxyisobutyric acid by engineered Cupriavidus necator strains","volume":"99","author":"Przybylski","year":"2015","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.biortech.2025.133407_b0115","series-title":"3rd IAASS Conference paper","article-title":"Modeling the risk of fire\/explosion due to oxidizer\/fuel leaks in the Ares I interstage","author":"Ring","year":"2008"},{"key":"10.1016\/j.biortech.2025.133407_b0099","doi-asserted-by":"crossref","unstructured":"Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (Eds.), 2013. The prokaryotes: Applied bacteriology and biotechnology, 4. ed. ed. SpringerLink B\u00fccher. Springer, Berlin, Heidelberg, 394 pp.","DOI":"10.1007\/978-3-642-31331-8"},{"key":"10.1016\/j.biortech.2025.133407_b0120","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1186\/s12934-023-02265-1","article-title":"The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions","volume":"22","author":"Sakarika","year":"2023","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.biortech.2025.133407_b9020","volume":"28","author":"Schoenmakers","year":"2025","journal-title":"Isopropanol production from carbon dioxide by Cupriavidus necator using a zero-gap cell with culture broth as catholyte. iScience."},{"key":"10.1016\/j.biortech.2025.133407_b0125","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.bej.2014.01.010","article-title":"Syngas fermentation of Clostridium carboxidivoran P7 in a hollow fiber membrane biofilm reactor: Evaluating the mass transfer coefficient and ethanol production performance","volume":"85","author":"Shen","year":"2014","journal-title":"Biochem Eng J"},{"key":"10.1016\/j.biortech.2025.133407_b0130","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1002\/bit.260260721","article-title":"Kinetics of Growth of the Hydrogen-Oxidizing Bacterium Alcaligenes eutrophus (ATCC 17707) in Chemostat Culture","volume":"26","author":"Siegel","year":"1984","journal-title":"Biotechnol Bioeng"},{"issue":"4","key":"10.1016\/j.biortech.2025.133407_b0135","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/bmb.2006.494034042627","article-title":"Pentose phosphate and Calvin cycles: Similarities and three-dimensional views","volume":"34","author":"Sillero","year":"2006","journal-title":"Biochem. Mol. Biol. Edu."},{"key":"10.1016\/j.biortech.2025.133407_b0140","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.enpol.2017.12.007","article-title":"Aviation CO2 emissions reductions from the use of alternative jet fuels","volume":"114","author":"Staples","year":"2018","journal-title":"Energy Policy"},{"issue":"6293","key":"10.1016\/j.biortech.2025.133407_b0145","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1126\/science.aaf7271","article-title":"How climate change affects extreme weather events","volume":"352","author":"Stott","year":"2016","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.biortech.2025.133407_b9010","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.jbiosc.2021.08.004","article-title":"Isopropanol production with reutilization of glucose-derived CO 2 by engineered Ralstonia eutropha","volume":"132","author":"Subagyo","year":"2021","journal-title":"J. Biosc. Bioeng."},{"key":"10.1016\/j.biortech.2025.133407_b0150","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.3390\/bioengineering10101194","article-title":"Autotrophic Production of the Sesquiterpene \u03b1-Humulene with Cupriavidus necator in a Controlled Bioreactor","volume":"10","author":"Sydow","year":"2023","journal-title":"Bioengineering"},{"issue":"8","key":"10.1016\/j.biortech.2025.133407_b9035","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1073\/pnas.1424872112","article-title":"Efficient solar-to-fuels production from a hybrid microbial-water-splitting catalyst system","volume":"112","author":"Torella","year":"2015","journal-title":"PNAS."},{"key":"10.1016\/j.biortech.2025.133407_b0155","doi-asserted-by":"crossref","first-page":"10065","DOI":"10.1007\/s00253-016-7844-6","article-title":"Footprint area analysis of binary imaged Cupriavidus necator cells to study PHB production at balanced, transient, and limitedgrowth conditions in a cascade process","volume":"100","author":"Vadlja","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.biortech.2025.133407_b0160","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.resmic.2012.10.008","article-title":"Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological\u2013biochemical properties and growth of Ralstonia eutropha","volume":"164","author":"Volova","year":"2013","journal-title":"Res Microbiol"},{"key":"10.1016\/j.biortech.2025.133407_b0165","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.biortech.2018.07.098","article-title":"Mixed culture 483 fermentation of synthesis gas in the microfiltration and ultrafiltration hollow-fiber membrane biofilm reactors","volume":"267","author":"Wang","year":"2018","journal-title":"Biores. Technol."},{"key":"10.1016\/j.biortech.2025.133407_b0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2025.03.011","article-title":"Comparative characterisation of autotrophic and heterotrophic isopropanol formation by Cupriavidus necator in shake flasks","volume":"403","author":"Weickardt","year":"2025","journal-title":"J Biotechnol"},{"key":"10.1016\/j.biortech.2025.133407_b0175","doi-asserted-by":"crossref","first-page":"5983","DOI":"10.1002\/pmic.200600244","article-title":"cytomic approach reveals population heterogeneity of Cupriavidus necator in response to harmful phenol concentrations","volume":"6","author":"Wiacek","year":"2006","journal-title":"Proteomics"}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425013744?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425013744?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T20:31:11Z","timestamp":1762633871000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852425013744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":40,"alternative-id":["S0960852425013744"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133407","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Aerobic autotrophic isopropanol production under low H2 atmosphere in Hollow Fiber Membrane Bioreactor","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133407","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"133407"}}