{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T03:40:43Z","timestamp":1788838843141,"version":"build-2803163510"},"reference-count":84,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.biortech.2025.133574","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T07:57:45Z","timestamp":1761638265000},"page":"133574","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Biotechnology of isopentenols production from microbial cell factories"],"prefix":"10.1016","volume":"441","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2964-2205","authenticated-orcid":false,"given":"Suresh Chandra","family":"Phulara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratima","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"Bhadri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1835-2755","authenticated-orcid":false,"given":"Ashish","family":"Runthala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2025.133574_b0005","doi-asserted-by":"crossref","first-page":"15434","DOI":"10.1021\/acssuschemeng.9b02928","article-title":"Greenhouse gas footprint, water-intensity, and production cost of bio-based isopentenol as a renewable transportation fuel","volume":"7","author":"Baral","year":"2019","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0010","doi-asserted-by":"crossref","first-page":"19949","DOI":"10.1073\/pnas.1106958108","article-title":"Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli","volume":"108","author":"Bokinsky","year":"2011","journal-title":"PNAS"},{"key":"10.1016\/j.biortech.2025.133574_b0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cej.2015.11.024","article-title":"Gas stripping-pervaporation hybrid process for energy-saving product recovery from acetone-butanol-ethanol (ABE) fermentation broth","volume":"287","author":"Cai","year":"2016","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2025.133574_b0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymben.2025.01.009","article-title":"Prenol production in a microbial host via the \u201crepass\u201d pathways","author":"Carruthers","year":"2025","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-018-0913-z","article-title":"Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis","volume":"17","author":"Chen","year":"2018","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.biortech.2025.133574_b0030","doi-asserted-by":"crossref","first-page":"15021","DOI":"10.1039\/C8RA01783B","article-title":"Directed evolution of mevalonate kinase in Escherichia coli by random mutagenesis for improved lycopene","volume":"8","author":"Chen","year":"2018","journal-title":"RSC Adv."},{"key":"10.1016\/j.biortech.2025.133574_b0035","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/s12934-019-1246-2","article-title":"High production of valencene in Saccharomyces cerevisiae through metabolic engineering","volume":"18","author":"Chen","year":"2019","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.biortech.2025.133574_b0040","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1021\/bi9017957","article-title":"Characterization of thermophilic archaeal isopentenyl phosphate kinases","volume":"49","author":"Chen","year":"2010","journal-title":"Biochemistry"},{"key":"10.1016\/j.biortech.2025.133574_b0045","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1186\/s13068-016-0617-8","article-title":"Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria","volume":"9","author":"Choi","year":"2016","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0050","doi-asserted-by":"crossref","first-page":"7849","DOI":"10.1128\/AEM.01175-12","article-title":"Synthetic pathway for production of five-carbon alcohols from isopentenyl diphosphate","volume":"78","author":"Chou","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0055","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1002\/bbb.1949","article-title":"Techno-economic assessment of the use of solvents in the scale-up of microbial sesquiterpene production for fuels and fine chemicals","volume":"13","author":"de la Cuesta","year":"2019","journal-title":"Biofuels Bioprod. Biorefining."},{"key":"10.1016\/j.biortech.2025.133574_b0060","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3390\/fermentation7010026","article-title":"In situ product recovery of bio-based industrial platform chemicals: a guideline to solvent selection","volume":"7","author":"De Brabander","year":"2021","journal-title":"Fermentation"},{"key":"10.1016\/j.biortech.2025.133574_b0065","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1021\/acssuschemeng.7b03530","article-title":"Platform study on the development of a nondetoxified rice straw hydrolysate to its application in lipid production from Mortierella alpina","volume":"6","author":"Diwan","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0070","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1038\/nbt.2055","article-title":"Relative potential of biosynthetic pathways for biofuels and bio-based products","volume":"29","author":"Dugar","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0075","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1754-6834-4-32","article-title":"Engineering microbes for tolerance to next-generation biofuels","volume":"4","author":"Dunlop","year":"2011","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0080","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/msb.2011.21","article-title":"Engineering microbial biofuel tolerance and export using efflux pumps","volume":"7","author":"Dunlop","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.biortech.2025.133574_b0085","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1046\/j.1462-2920.2000.00095.x","article-title":"Bacterial sources and sinks of isoprene , a reactive atmospheric hydrocarbo","volume":"2","author":"Fall","year":"2000","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0090","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1128\/mBio.01932-14","article-title":"Improving microbial biogasoline production in Escherichia coli using tolerance engineering","volume":"5","author":"Foo","year":"2014","journal-title":"MBio"},{"key":"10.1016\/j.biortech.2025.133574_b0095","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jbiotec.2007.08.004","article-title":"Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium","volume":"132","author":"F\u00fcrch","year":"2007","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0100","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1039\/C5EE03102H","article-title":"Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2","volume":"9","author":"Gao","year":"2016","journal-title":"Energy Environ. Sci."},{"key":"10.1016\/j.biortech.2025.133574_b0105","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1002\/bit.25226","article-title":"Correlation analysis of targeted proteins and metabolites to assess and engineer microbial isopentenol production","volume":"111","author":"George","year":"2014","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2025.133574_b0110","doi-asserted-by":"crossref","first-page":"11128","DOI":"10.1038\/srep11128","article-title":"Metabolic engineering for the high-yield production of isoprenoid-based C 5 alcohols in E. coli","volume":"5","author":"George","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biortech.2025.133574_b0115","series-title":"Biotechnology of terpenoid production from microbial cell factories","author":"Gupta","year":"2021"},{"key":"10.1016\/j.biortech.2025.133574_b0120","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202300710","article-title":"Proteomic analysis reveals that the co-ordination of cytosolic and mitochondrial pathways is beneficial for sabinene biosynthesis in engineered Saccharomyces cerevisiae","volume":"19","author":"Hou","year":"2024","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.biortech.2025.133574_b0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2021.112803","article-title":"Maternal exposure to polystyrene nanoplastics during gestation and lactation induces hepatic and testicular toxicity in male mouse offspring","volume":"160","author":"Huang","year":"2022","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.biortech.2025.133574_b0130","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1021\/acssynbio.8b00398","article-title":"Machine learning of designed translational control allows predictive pathway optimization in Escherichia coli","volume":"8","author":"Jervis","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2025.133574_b0135","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.carbpol.2015.12.065","article-title":"Efficient biosynthesis of polysaccharides chondroitin and heparosan by metabolically engineered Bacillus subtilis","volume":"140","author":"Jin","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.biortech.2025.133574_b0140","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ymben.2015.12.002","article-title":"Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production","volume":"34","author":"Kang","year":"2016","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0145","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ymben.2017.03.010","article-title":"High-throughput enzyme screening platform for the IPP-bypass mevalonate pathway for isopentenol production","volume":"41","author":"Kang","year":"2017","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0150","doi-asserted-by":"crossref","unstructured":"Khana, D.B., Tatli, M., Vazquez, J.R., Weraduwage, S.M., Stern, N., Hebert, A.S., Trujillo, E.A., Stevenson, D.M., Coon, J.J., Sharky, T.D., Amador-Noguez, D., 2023. Systematic analysis of metabolic bottlenecks in the methylerythritol 4-phosphate (mep) pathway of Zymomonas mobilis. mSystems 8. https:\/\/doi.org\/10.1128\/MSYSTEMS.00092-23.","DOI":"10.1128\/msystems.00092-23"},{"key":"10.1016\/j.biortech.2025.133574_b0155","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.ymben.2021.02.002","article-title":"Engineering Saccharomyces cerevisiae for isoprenol production","volume":"64","author":"Kim","year":"2021","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0160","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1296132","article-title":"Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli","volume":"11","author":"Kim","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0165","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.bbrc.2017.02.066","article-title":"Isoamyl alcohol odor promotes longevity and stress tolerance via DAF-16 in Caenorhabditis elegans","volume":"485","author":"Kurino","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.biortech.2025.133574_b0170","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/BF00294190","article-title":"Bacteria produce the volatile hydrocarbon isoprene","volume":"30","author":"Kuzma","year":"1995","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0175","doi-asserted-by":"crossref","first-page":"13172","DOI":"10.1073\/pnas.240454797","article-title":"Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes","volume":"97","author":"Lange","year":"2000","journal-title":"PNAS"},{"key":"10.1016\/j.biortech.2025.133574_b0180","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13065-017-0247-7","article-title":"Identification of the aroma compounds in Vitex doniana sweet: free and bound odorants","volume":"11","author":"Lasekan","year":"2017","journal-title":"Chem. Cent. J."},{"key":"10.1016\/j.biortech.2025.133574_b0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.130988","article-title":"Natural transformation of Vibrio natriegens with large genetic cluster enables alginate assimilation for isopentenol production","volume":"406","author":"Lee","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.133574_b0190","doi-asserted-by":"crossref","first-page":"7725","DOI":"10.1007\/s00253-018-9200-5","article-title":"Metabolic engineering for the production of isoprene and isopentenol by Escherichia coli","volume":"102","author":"Li","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0195","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s00425-018-3047-y","article-title":"Engineering cyanobacteria for production of terpenoids","volume":"249","author":"Lin","year":"2019","journal-title":"Planta"},{"key":"10.1016\/j.biortech.2025.133574_b0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.mec.2021.e00164","article-title":"Enhanced limonene production in a fast-growing cyanobacterium through combinatorial metabolic engineering","volume":"12","author":"Lin","year":"2021","journal-title":"Metab. Eng. Commun."},{"key":"10.1016\/j.biortech.2025.133574_b0205","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s12934-014-0135-y","article-title":"MEP Pathway-mediated isopentenol production in metabolically engineered Escherichia coli","volume":"13","author":"Liu","year":"2014","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.biortech.2025.133574_b0210","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1002\/bit.26034","article-title":"Combinatorial pathway optimization in Escherichia coli by directed co-evolution of rate-limiting enzymes and modular pathway engineering","volume":"113","author":"Lv","year":"2016","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2025.133574_b0215","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.copbio.2021.02.008","article-title":"Heterologous expression and metabolic engineering tools for improving terpenoids production","volume":"69","author":"Ma","year":"2021","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0220","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.fuel.2013.10.024","article-title":"Investigation of biofuels from microorganism metabolism for use as anti-knock additives","volume":"117","author":"Mack","year":"2014","journal-title":"Fuel"},{"key":"10.1016\/j.biortech.2025.133574_b0225","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/nbt833","article-title":"Engineering a mevalonate pathway in Escherichia coli for production of terpenoids","volume":"21","author":"Martin","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.biortech.2025.133574_b0230","article-title":"Quantitative design of regulatory elements based on high-precision strength prediction using artificial neural network","volume":"8","author":"Meng","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.biortech.2025.133574_b0235","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/B978-0-444-63455-9.50115-X","article-title":"Techno-economic analysis of a thermochemical lignocellulosic biomass-to-butanol process","volume":"33","author":"Okoli","year":"2014","journal-title":"Comput. Aided Chem. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0240","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.3390\/pr13072115","article-title":"Advancements in metabolic engineering: enhancing biofuel production through Escherichia coli and Saccharomyces cerevisiae Models","volume":"13","author":"Pabbathi","year":"2025","journal-title":"Processes"},{"key":"10.1016\/j.biortech.2025.133574_b0245","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1080\/09637486.2018.1554031","article-title":"3-Methyl-3-buten-1-ol (isoprenol) confers longevity and stress tolerance in Caenorhabditis elegans","volume":"70","author":"Pandey","year":"2019","journal-title":"Int. J. Food Sci. Nutr."},{"key":"10.1016\/j.biortech.2025.133574_b0250","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ymben.2018.08.002","article-title":"Metabolic engineering of Escherichia coli for high-level astaxanthin production with high productivity","volume":"49","author":"Park","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0255","doi-asserted-by":"crossref","DOI":"10.1016\/j.mec.2020.e00125","article-title":"Introduction of a green algal squalene synthase enhances squalene accumulation in a strain of Synechocystis sp PCC 6803","volume":"10","author":"Pattanaik","year":"2020","journal-title":"Metab. Eng. Commun."},{"key":"10.1016\/j.biortech.2025.133574_b0260","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-024-49483-8","article-title":"The methylerythritol phosphate pathway as an oxidative stress sense and response system","volume":"15","author":"Perez-Gil","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2025.133574_b0265","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1021\/sb5002517","article-title":"Engineering terpene biosynthesis in streptomyces for production of the advanced biofuel precursor bisabolene","volume":"4","author":"Phelan","year":"2014","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2025.133574_b0270","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jbiosc.2018.10.002","article-title":"Modulation of culture medium confers high-specificity production of isopentenol in Bacillus subtilis","volume":"127","author":"Phulara","year":"2018","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.biortech.2025.133574_b0275","doi-asserted-by":"crossref","first-page":"5730","DOI":"10.1128\/AEM.01192-16","article-title":"Isoprenoid-based biofuels: Homologous expression and heterologous expression in prokaryotes","volume":"82","author":"Phulara","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0280","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.procbio.2018.06.019","article-title":"In-situ isopentenol production from Bacillus subtilis through genetic and culture condition modulation","volume":"72","author":"Phulara","year":"2018","journal-title":"Process Biochem."},{"key":"10.1016\/j.biortech.2025.133574_b0285","article-title":"Hemiterpene compound, 3,3-dimethylallyl alcohol promotes longevity and neuroprotection in Caenorhabditis elegans","volume":"791\u2013807","author":"Phulara","year":"2021","journal-title":"GeroScience"},{"key":"10.1016\/j.biortech.2025.133574_b0290","doi-asserted-by":"crossref","first-page":"19898","DOI":"10.1021\/acsomega.2c01677","article-title":"Computationally decoding NudFresidues to enhance the yield of the DXP pathway","volume":"7","author":"Prasanna","year":"2022","journal-title":"ACS Omega"},{"key":"10.1016\/j.biortech.2025.133574_b0295","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1039\/D1NP00025J","article-title":"Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli","volume":"39","author":"Rinaldi","year":"2022","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.biortech.2025.133574_b0300","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11101-005-3130-4","article-title":"Early steps in isoprenoid biosynthesis: multilevel regulation of the supply of common precursors in plant cells","volume":"5","author":"Rodr\u00edguez-Concepci\u00f3n","year":"2006","journal-title":"Phytochem. Rev."},{"key":"10.1016\/j.biortech.2025.133574_b0305","doi-asserted-by":"crossref","first-page":"667","DOI":"10.3390\/pr7100667","article-title":"A feasibility study of cellulosic isobutanol production\u2014process simulation and economic analysis","volume":"7","author":"Roussos","year":"2019","journal-title":"Processes"},{"key":"10.1016\/j.biortech.2025.133574_b0310","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-019-1381-3","article-title":"Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates","volume":"12","author":"Sasaki","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0315","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1021\/acs.jafc.7b00473","article-title":"Microbial cell factories for the production of terpenoid flavor and fragrance compounds","volume":"66","author":"Schempp","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2025.133574_b0320","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1007\/s12155-015-9588-z","article-title":"Impact of pretreatment technologies on saccharification and isopentenol fermentation of mixed lignocellulosic feedstocks","volume":"8","author":"Shi","year":"2015","journal-title":"Bioenergy Res."},{"key":"10.1016\/j.biortech.2025.133574_b0325","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202300285","article-title":"Building a machine-learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast","volume":"19","author":"Shimazaki","year":"2024","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.biortech.2025.133574_b0330","doi-asserted-by":"crossref","first-page":"e02369","DOI":"10.1128\/AEM.02369-20","article-title":"Impact of culture condition modulation on the high-yield, high-specificity, and cost-effective production of terpenoids from microbial sources: a review","volume":"87","author":"Shukla","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0335","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1021\/acssynbio.8b00429","article-title":"Redirecting metabolic flux via combinatorial multiplex crispri-mediated repression for isopentenol production in Escherichia coli","volume":"8","author":"Tian","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2025.133574_b0340","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1038\/nature08187","article-title":"Programming cells by multiplex genome engineering and accelerated evolution","volume":"460","author":"Wang","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2025.133574_b0345","doi-asserted-by":"crossref","first-page":"6955","DOI":"10.1039\/D2GC02255A","article-title":"Improving isoprenol production via systematic CRISPRi screening in engineered Escherichia coli","volume":"24","author":"Wang","year":"2022","journal-title":"Green Chem."},{"key":"10.1016\/j.biortech.2025.133574_b0350","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biteb.2019.01.021","article-title":"NaCl enhances Escherichia coli growth and isoprenol production in the presence of imidazolium-based ionic liquids","volume":"6","author":"Wang","year":"2019","journal-title":"Bioresour. Technol. Reports"},{"key":"10.1016\/j.biortech.2025.133574_b0355","article-title":"Recombineering and MAGE","volume":"1","author":"Wannier","year":"2021","journal-title":"Nat. Rev. Methods Prim."},{"key":"10.1016\/j.biortech.2025.133574_b0360","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-018-1137-5","article-title":"Integrated in situ gas stripping-salting-out process for high-titer acetone-butanol-ethanol production from sweet sorghum bagasse","volume":"11","author":"Wen","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0365","doi-asserted-by":"crossref","first-page":"6277","DOI":"10.1128\/AEM.00861-07","article-title":"Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity","volume":"73","author":"Withers","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2025.133574_b0370","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1039\/D2YA00017B","article-title":"Thermal cyclodimerization of isoprene for the production of high-performance sustainable aviation fuel","volume":"1","author":"Woodroffe","year":"2022","journal-title":"Energy Adv."},{"key":"10.1016\/j.biortech.2025.133574_b0375","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-018-1285-7","article-title":"Synthesis and techno-economic assessment of microbial-based processes for terpenes production","volume":"11","author":"Wu","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0380","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3390\/membranes14110234","article-title":"Organic solvent nanofiltration membrane with in-situ constructed covalent organic frameworks as separation layer","volume":"14","author":"Xu","year":"2024","journal-title":"Membranes (Basel)."},{"key":"10.1016\/j.biortech.2025.133574_b0385","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-024-54897-5","article-title":"Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis","volume":"15","author":"Yan","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2025.133574_b0390","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12896-016-0236-2","article-title":"A novel MVA-mediated pathway for isoprene production in engineered E. coli","volume":"16","author":"Yang","year":"2016","journal-title":"BMC Biotech."},{"key":"10.1016\/j.biortech.2025.133574_b0395","article-title":"Combining genotype improvement and statistical media optimization for isoprenoid production in E . col","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.biortech.2025.133574_b0400","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.ymben.2013.02.002","article-title":"Engineering central metabolic modules of Escherichia coli for improving beta-carotene production","volume":"17","author":"Zhao","year":"2013","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2025.133574_b0405","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/1754-6834-6-57","article-title":"Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels","volume":"6","author":"Zheng","year":"2013","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.biortech.2025.133574_b0410","article-title":"Metabolite profiling identified methylerythritol cyclodiphosphate efflux as a limiting step in microbial isoprenoid production","volume":"7","author":"Zhou","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.biortech.2025.133574_b0415","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/1475-2859-11-148","article-title":"Enhancing solubility of deoxyxylulose phosphate pathway enzymes for microbial isoprenoid production","volume":"11","author":"Zhou","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.biortech.2025.133574_b0420","doi-asserted-by":"crossref","first-page":"2556","DOI":"10.1002\/bit.24900","article-title":"Optimization of amorphadiene synthesis in Bacillus subtilis via transcriptional, translational, and media modulation","volume":"110","author":"Zhou","year":"2013","journal-title":"Biotechnol. Bioeng."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096085242501541X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096085242501541X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T19:53:15Z","timestamp":1765137195000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096085242501541X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":84,"alternative-id":["S096085242501541X"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133574","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biotechnology of isopentenols production from microbial cell factories","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133574","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"133574"}}