{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:57:55Z","timestamp":1778043475056,"version":"3.51.4"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32030003"],"award-info":[{"award-number":["32030003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012336","name":"973 Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012336","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD2101203"],"award-info":[{"award-number":["2022YFD2101203"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.biortech.2025.133803","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:26:49Z","timestamp":1765931209000},"page":"133803","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Single-cell phenotyping and sequencing uncover metabolically active low-abundance yeasts in thermophilic fermentation"],"prefix":"10.1016","volume":"443","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7411-4893","authenticated-orcid":false,"given":"Huaizhi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1228-8710","authenticated-orcid":false,"given":"Xiaoyan","family":"Jing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhai","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yishang","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongze","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YuMei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuziyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.biortech.2025.133803_b0005","doi-asserted-by":"crossref","first-page":"e00152","DOI":"10.1128\/spectrum.00152-24","article-title":"Proteome signatures reveal homeostatic and adaptive oxidative responses by a putative co-chaperone, Wos2, to influence fungal virulence determinants in cryptococcosis","volume":"12","author":"Ball","year":"2024","journal-title":"Microbiol. Spectr."},{"issue":"8","key":"10.1016\/j.biortech.2025.133803_b0010","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.tibtech.2023.03.002","article-title":"Analyzing and understanding the robustness of bioprocesses","volume":"41","author":"Becker","year":"2023","journal-title":"Trends Biotechnol."},{"issue":"2","key":"10.1016\/j.biortech.2025.133803_b0015","doi-asserted-by":"crossref","first-page":"E194","DOI":"10.1073\/pnas.1420406112","article-title":"Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells","volume":"112","author":"Berry","year":"2015","journal-title":"PNAS"},{"issue":"2","key":"10.1016\/j.biortech.2025.133803_b0020","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1038\/s41564-020-00816-5","article-title":"Metabolic cooperation and spatiotemporal niche partitioning in a kefir microbial community","volume":"6","author":"Blasche","year":"2021","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.biortech.2025.133803_b0025","series-title":"Examining FLO gene expression of Saccharomyces cerevisiae in the presence of selected non-Saccharomyces strains","author":"Brown","year":"2022"},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0030","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s40168-022-01301-3","article-title":"Metabolic framework of spontaneous and synthetic sourdough metacommunities to reveal microbial players responsible for resilience and performance","volume":"10","author":"Calabrese","year":"2022","journal-title":"Microbiome"},{"issue":"1\u20133","key":"10.1016\/j.biortech.2025.133803_b0035","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.cbi.2012.12.006","article-title":"Biocatalytic production of alpha-hydroxy ketones and vicinal diols by yeast and human aldo\u2013keto reductases","volume":"202","author":"Calam","year":"2013","journal-title":"Chem. Biol. Interact."},{"issue":"10","key":"10.1016\/j.biortech.2025.133803_b0040","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/s41579-022-00682-4","article-title":"Tolerance and resistance of microbial biofilms","volume":"20","author":"Ciofu","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"issue":"2","key":"10.1016\/j.biortech.2025.133803_b0045","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/brv.13024","article-title":"Cathemerality: a key temporal niche","volume":"99","author":"Cox","year":"2024","journal-title":"Biol. Rev."},{"key":"10.1016\/j.biortech.2025.133803_b0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.soilbio.2020.107873","article-title":"Temperatures beyond the community optimum promote the dominance of heat-adapted, fast growing and stress resistant bacteria in alpine soils","volume":"148","author":"Donhauser","year":"2020","journal-title":"Soil Biol. Biochem."},{"issue":"3","key":"10.1016\/j.biortech.2025.133803_b0055","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/s41396-019-0557-y","article-title":"Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere","volume":"14","author":"Doud","year":"2020","journal-title":"ISME J."},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0060","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s40168-017-0285-3","article-title":"Schr\u00f6dinger\u2019s microbes: tools for distinguishing the living from the dead in microbial ecosystems","volume":"5","author":"Emerson","year":"2017","journal-title":"Microbiome"},{"issue":"6","key":"10.1016\/j.biortech.2025.133803_b0065","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.jbiosc.2015.11.002","article-title":"Advances in mechanisms and modifications for rendering yeast thermotolerance","volume":"121","author":"Gao","year":"2016","journal-title":"J. Biosci. Bioeng."},{"issue":"20","key":"10.1016\/j.biortech.2025.133803_b0070","doi-asserted-by":"crossref","first-page":"11322","DOI":"10.1002\/ece3.6770","article-title":"Resource competition shapes biological rhythms and promotes temporal niche differentiation in a community simulation","volume":"10","author":"Gao","year":"2020","journal-title":"Ecol. Evol."},{"issue":"4","key":"10.1016\/j.biortech.2025.133803_b0075","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01470-21","article-title":"Intra-Ramanome Correlation Analysis unveils metabolite conversion network from an isogenic population of cells","volume":"12","author":"He","year":"2021","journal-title":"MBio"},{"key":"10.1016\/j.biortech.2025.133803_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.tifs.2024.104569","article-title":"The future is fermented: Microbial biodiversity of fermented foods is a critical resource for food innovation and human health","volume":"150","author":"Hern\u00e1ndez-Vel\u00e1zquez","year":"2024","journal-title":"Trends Food Sci. Technol."},{"key":"10.1016\/j.biortech.2025.133803_b0085","series-title":"Biotechnology of Yeasts and Filamentous Fungi","first-page":"49","article-title":"Thermotolerant yeasts and high-temperature fermentation","author":"Hoshida","year":"2025"},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0090","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1038\/s41467-024-46920-6","article-title":"Survival and rapid resuscitation permit limited productivity in desert microbial communities","volume":"15","author":"Imminger","year":"2024","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0095","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/s43705-022-00188-3","article-title":"Single-cell Raman-activated sorting and cultivation (scRACS-culture) for assessing and mining in situ phosphate-solubilizing microbes from nature","volume":"2","author":"Jing","year":"2022","journal-title":"ISME Commun."},{"issue":"3","key":"10.1016\/j.biortech.2025.133803_b0100","doi-asserted-by":"crossref","first-page":"e00181","DOI":"10.1128\/mSystems.00181-21","article-title":"One-cell metabolic phenotyping and sequencing of soil microbiome by Raman-activated gravity-driven encapsulation (RAGE)","volume":"6","author":"Jing","year":"2021","journal-title":"mSystems"},{"issue":"3","key":"10.1016\/j.biortech.2025.133803_b0105","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/yea.1349","article-title":"Cell wall construction in Saccharomyces cerevisiae","volume":"23","author":"Klis","year":"2006","journal-title":"Yeast"},{"issue":"8","key":"10.1016\/j.biortech.2025.133803_b0110","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/s43016-024-01024-8","article-title":"Single-cell exploration of active phosphate-solubilizing bacteria across diverse soil matrices for sustainable phosphorus management","volume":"5","author":"Li","year":"2024","journal-title":"Nat. Food"},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0115","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s40793-023-00490-0","article-title":"Belowground microbiota analysis indicates that Fusarium spp. exacerbate grapevine trunk disease","volume":"18","author":"Li","year":"2023","journal-title":"Environ. Microbiome"},{"key":"10.1016\/j.biortech.2025.133803_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2024.108422","article-title":"Yeast surface display technology: mechanisms, applications, and perspectives","volume":"76","author":"Li","year":"2024","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.biortech.2025.133803_b0125","doi-asserted-by":"crossref","unstructured":"Ma, S., Li, Y., Chen, C., Dong, Y., Huang, P., Tu, R., Liu, X., Zhou, R., Wu, C., 2025. Metabolic interactions drive microbial community succession and functional expression of Nongxiangxing (Strong-flavor) daqu. J. Adv. Res., in press. https:\/\/doi.org\/10.1016\/j.jare.2025.09.052.","DOI":"10.1016\/j.jare.2025.09.052"},{"key":"10.1016\/j.biortech.2025.133803_b0130","doi-asserted-by":"crossref","first-page":"e8783","DOI":"10.7717\/peerj.8783","article-title":"Meta-SourceTracker: application of Bayesian source tracking to shotgun metagenomics","volume":"8","author":"McGhee","year":"2020","journal-title":"PeerJ"},{"issue":"12","key":"10.1016\/j.biortech.2025.133803_b0135","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.3390\/genes13122341","article-title":"Dynamic transcriptome analysis reveals transcription factors involved in the synthesis of ethyl acetate in aroma-producing yeast","volume":"13","author":"Ni","year":"2022","journal-title":"Genes"},{"key":"10.1016\/j.biortech.2025.133803_b0140","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foaf047","article-title":"Thermotolerant yeasts promoting climate-resilient bioproduction","volume":"25","author":"Shirvani","year":"2025","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.biortech.2025.133803_b0145","series-title":"Extremophilic Yeasts","first-page":"33","article-title":"Mechanisms of thermotolerance and thermotolerant yeasts: properties and applications","author":"Sibirny","year":"2025"},{"issue":"9","key":"10.1016\/j.biortech.2025.133803_b0150","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1038\/ismej.2017.59","article-title":"Capturing the genetic makeup of the active microbiome in situ","volume":"11","author":"Singer","year":"2017","journal-title":"ISME J."},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0155","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tim.2023.06.006","article-title":"Microbial regulation of feedbacks to ecosystem change","volume":"32","author":"Sveen","year":"2024","journal-title":"Trends Microbiol."},{"issue":"5","key":"10.1016\/j.biortech.2025.133803_b0160","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/1758-2229.12776","article-title":"Towards PacBio-based pan-eukaryote metabarcoding using full-length ITS sequences","volume":"11","author":"Tedersoo","year":"2019","journal-title":"Environ. Microbiol. Rep."},{"issue":"2","key":"10.1016\/j.biortech.2025.133803_b0165","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1111\/j.1567-1364.2008.00462.x","article-title":"Phenotypic diversity of Flo protein family-mediated adhesion in Saccharomyces cerevisiae","volume":"9","author":"Van Mulders","year":"2009","journal-title":"FEMS Yeast Res."},{"issue":"4","key":"10.1016\/j.biortech.2025.133803_b0170","doi-asserted-by":"crossref","first-page":"e00555","DOI":"10.1128\/mSystems.00555-20","article-title":"Temperature-induced annual variation in microbial community changes and resulting metabolome shifts in a controlled fermentation system","volume":"5","author":"Wang","year":"2020","journal-title":"mSystems"},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0175","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/s41467-019-09267-x","article-title":"Assessing the viability of transplanted gut microbiota by sequential tagging with d-amino acid-based metabolic probes","volume":"10","author":"Wang","year":"2019","journal-title":"Nat. Commun."},{"issue":"16","key":"10.1016\/j.biortech.2025.133803_b0180","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202370102","article-title":"Robust spontaneous Raman flow cytometry for single-cell metabolic phenome profiling via pDEP-DLD-RFC","volume":"10","author":"Wang","year":"2023","journal-title":"Adv. Sci."},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0185","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s40168-022-01449-y","article-title":"RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities","volume":"11","author":"Wang","year":"2023","journal-title":"Microbiome"},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0190","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s40168-020-00961-3","article-title":"Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach","volume":"9","author":"Wang","year":"2021","journal-title":"Microbiome"},{"issue":"50","key":"10.1016\/j.biortech.2025.133803_b0195","doi-asserted-by":"crossref","first-page":"20295","DOI":"10.1021\/acs.jafc.3c06246","article-title":"Serine improves lactic acid stress tolerance and ethanol production in Zygosaccharomyces bailii in baijiu fermentation","volume":"71","author":"Wei","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2025.133803_b0200","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1007\/s43393-025-00368-x","article-title":"Improved fungal collection supports the identification and function evaluation of culturable but not sequenced (CBNS) fungi in high-temperature Daqu","volume":"5","author":"Wu","year":"2025","journal-title":"Syst. Microbiol. Biomanuf."},{"issue":"14","key":"10.1016\/j.biortech.2025.133803_b0205","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202217412","article-title":"An isotope-labeled single-cell Raman spectroscopy approach for tracking the physiological evolution trajectory of bacteria toward antibiotic resistance","volume":"62","author":"Yang","year":"2023","journal-title":"Angew. Chem. Int. Ed."},{"issue":"11","key":"10.1016\/j.biortech.2025.133803_b0210","doi-asserted-by":"crossref","first-page":"3558","DOI":"10.1021\/acs.jafc.9b07809","article-title":"The biosynthesis mechanism involving 2,3-pentanedione and aminoacetone describes the production of 2-ethyl-3,5-dimethylpyrazine and 2-ethyl-3,6-dimethylpyrazine by Bacillus subtilis","volume":"68","author":"Zhang","year":"2020","journal-title":"J. Agric. Food Chem."},{"issue":"1","key":"10.1016\/j.biortech.2025.133803_b0215","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s40168-025-02044-7","article-title":"Soil warming increases the active antibiotic resistome in the gut of invasive giant African snails","volume":"13","author":"Zhang","year":"2025","journal-title":"Microbiome"},{"key":"10.1016\/j.biortech.2025.133803_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2022.111568","article-title":"Exploring the impacts of traditional crafts on microbial community succession in Jiang-flavored Daqu","volume":"158","author":"Zhu","year":"2022","journal-title":"Food Res. Int."},{"key":"10.1016\/j.biortech.2025.133803_b0225","article-title":"Microbiome catalog and dynamics of the chinese liquor fermentation process","author":"Zhu","year":"2025","journal-title":"Bioresour. Technol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425017705?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852425017705?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T03:20:11Z","timestamp":1771730411000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852425017705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":45,"alternative-id":["S0960852425017705"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133803","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell phenotyping and sequencing uncover metabolically active low-abundance yeasts in thermophilic fermentation","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133803","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"133803"}}