{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:35:17Z","timestamp":1790480117832,"version":"4.1.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100031935","name":"Xi&apos;an Jiaotong University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031935","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52488201"],"award-info":[{"award-number":["52488201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.biortech.2025.133900","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T17:02:32Z","timestamp":1767027752000},"page":"133900","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Integrated two-stage continuous system with pH-triggered CO2 delivery for sustainable algal wastewater-to-bioenergy production"],"prefix":"10.1016","volume":"443","author":[{"given":"Yuntong","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiyuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0664-382X","authenticated-orcid":false,"given":"Wen","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liejin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2025.133900_b0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.ese.2022.100205","article-title":"Microalgae-based wastewater treatment: mechanisms, challenges, recent advances, and future prospects","volume":"13","author":"Abdelfattah","year":"2023","journal-title":"Environ. Sci. Ecotechnol."},{"key":"10.1016\/j.biortech.2025.133900_b0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2672.2008.03918.x","article-title":"Applications of cyanobacteria in biotechnology","volume":"106","author":"Abed","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.biortech.2025.133900_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.rser.2019.109621","article-title":"Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: a review","volume":"119","author":"Aziz","year":"2020","journal-title":"Renew. Sustain. Energy Rev."},{"key":"10.1016\/j.biortech.2025.133900_b0020","doi-asserted-by":"crossref","unstructured":"Barabas, I., Todoru, I.-A., 2011. Biodiesel Quality, Standards and Properties, in: Montero, G. (Ed.), Biodiesel- Quality, Emissions and By-Products. InTech. https:\/\/doi.org\/10.5772\/25370.","DOI":"10.5772\/25370"},{"key":"10.1016\/j.biortech.2025.133900_b0025","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1093\/jxb\/ery141","article-title":"Inorganic carbon and pH dependency of photosynthetic rates in Trichodesmium","volume":"69","author":"Boatman","year":"2018","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.biortech.2025.133900_b0030","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41586-022-04662-9","article-title":"Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism","volume":"605","author":"Burlacot","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2025.133900_b0035","doi-asserted-by":"crossref","first-page":"7242","DOI":"10.1016\/j.jmrt.2024.04.239","article-title":"Corrosion behaviour and mechanism of acid-resistant steel in acidic solutions with different Cl\u2212 concentrations","volume":"30","author":"Chenghui","year":"2024","journal-title":"J. Mater. Res. Technol."},{"key":"10.1016\/j.biortech.2025.133900_b0040","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1016\/j.fuel.2007.08.007","article-title":"Relationships derived from physical properties of vegetable oil and biodiesel fuels","volume":"87","author":"Demirbas","year":"2008","journal-title":"Fuel"},{"key":"10.1016\/j.biortech.2025.133900_b0045","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.biortech.2025.133900_b0050","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nature11479","article-title":"Exploiting diversity and synthetic biology for the production of algal biofuels","volume":"488","author":"Georgianna","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2025.133900_b0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2021.131796","article-title":"Electrochemical pH control and carbon supply for microalgae cultivation","volume":"426","author":"Kim","year":"2021","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2025.133900_b0060","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.biortech.2018.01.153","article-title":"Quantification and characterisation of fatty acid methyl esters in microalgae: Comparison of pretreatment and purification methods","volume":"257","author":"Lage","year":"2018","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.133900_b0065","doi-asserted-by":"crossref","unstructured":"Li, D.-W., Balamurugan, S., Yang, Y.-F., Zheng, J.-W., Huang, D., Zou, L.-G., Yang, W.-D., Liu, J.-S., Guan, Y., Li, H.-Y., 2019. Transcriptional regulation of microalgae for concurrent lipid overproduction and secretion. Sci. Adv. https:\/\/doi.org\/10.1126\/sciadv.aau3795.","DOI":"10.1126\/sciadv.aau3795"},{"key":"10.1016\/j.biortech.2025.133900_b0070","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1016\/j.cell.2021.01.052","article-title":"Biofuels for a sustainable future","volume":"184","author":"Liu","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.biortech.2025.133900_b0075","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41467-021-27665-y","article-title":"Machine learning-informed and synthetic biology-enabled semi-continuous algal cultivation to unleash renewable fuel productivity","volume":"13","author":"Long","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2025.133900_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.renene.2023.119652","article-title":"Using nitrogen starvation and excess phosphorus for two-stage algae cultivation to improve butanol production of lipid-extracted algae","volume":"220","author":"Mao","year":"2024","journal-title":"Renew. Energy"},{"key":"10.1016\/j.biortech.2025.133900_b0085","doi-asserted-by":"crossref","first-page":"8803","DOI":"10.1021\/acs.est.3c10264","article-title":"Intensive microalgal cultivation and tertiary phosphorus recovery from wastewaters via the ecorecover process","volume":"58","author":"Molitor","year":"2024","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2025.133900_b0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2021.107836","article-title":"Genetic engineering of microalgae for enhanced lipid production","volume":"52","author":"Mu\u00f1oz","year":"2021","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.biortech.2025.133900_b0095","doi-asserted-by":"crossref","first-page":"12699","DOI":"10.1038\/ncomms12699","article-title":"Photon management for augmented photosynthesis","volume":"7","author":"Ooms","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2025.133900_b0100","doi-asserted-by":"crossref","first-page":"15366","DOI":"10.1021\/acs.est.0c02833","article-title":"Recycling of nutrients from dairy wastewater by extremophilic microalgae with high ammonia tolerance","volume":"54","author":"Pang","year":"2020","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2025.133900_b0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.rser.2021.111852","article-title":"Continuous cultivation of microalgae in photobioreactors as a source of renewable energy: current status and future challenges","volume":"154","author":"Peter","year":"2022","journal-title":"Renew. Sustain. Energy Rev."},{"key":"10.1016\/j.biortech.2025.133900_b0110","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1093\/jxb\/erm112","article-title":"Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants","volume":"59","author":"Price","year":"2008","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.biortech.2025.133900_b0115","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.algal.2017.11.004","article-title":"Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana","volume":"28","author":"Qiu","year":"2017","journal-title":"Algal Res."},{"key":"10.1016\/j.biortech.2025.133900_b0120","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.fuel.2011.06.070","article-title":"Predicting cetane number, kinematic viscosity, density and higher heating value of biodiesel from its fatty acid methyl ester composition","volume":"91","author":"Ram\u00edrez-Verduzco","year":"2012","journal-title":"Fuel"},{"key":"10.1016\/j.biortech.2025.133900_b0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2022.139754","article-title":"Valorization of micro-algae biomass for the development of green biorefinery: perspectives on techno-economic analysis and the way towards sustainability","volume":"453","author":"Saravanan","year":"2023","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2025.133900_b0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.resconrec.2022.106355","article-title":"Overview on stress-induced strategies for enhanced microalgae lipid production: application, mechanisms and challenges","volume":"183","author":"Song","year":"2022","journal-title":"Resour. Conserv. Recycl."},{"key":"10.1016\/j.biortech.2025.133900_b0135","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1128\/br.35.2.171-205.1971","article-title":"Purification and properties of unicellular blue-green algae (order Chroococcales)","volume":"35","author":"Stanier","year":"1971","journal-title":"Bacteriol. Rev."},{"key":"10.1016\/j.biortech.2025.133900_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131994","article-title":"Two-stage continuous cultivation of microalgae overexpressing cytochrome P450 improves nitrogen and antibiotics removal from livestock and poultry wastewater","volume":"418","author":"Xiao","year":"2025","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2025.133900_b0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2025.123145","article-title":"Revealing real impact of microalgae on seasonal dynamics of bacterial community in a pilot-scale microalgal-bacterial consortium system","volume":"274","author":"Xie","year":"2025","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2025.133900_b0150","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.biortech.2018.03.014","article-title":"Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism","volume":"259","author":"Zhao","year":"2018","journal-title":"Bioresour. Technol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096085242501867X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096085242501867X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T09:13:13Z","timestamp":1768986793000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096085242501867X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":30,"alternative-id":["S096085242501867X"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133900","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated two-stage continuous system with pH-triggered CO2 delivery for sustainable algal wastewater-to-bioenergy production","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2025.133900","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"133900"}}