{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:07:31Z","timestamp":1790208451865,"version":"4.1.0"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013804","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.biortech.2026.135460","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T07:57:08Z","timestamp":1784534228000},"page":"135460","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Efficient biosynthesis of \u0251-bisabolene in S. cerevisiae by combinatorial regulation of acetyl-CoA supply, bisabolene synthase expression, and bisabolene transportation"],"prefix":"10.1016","volume":"461","author":[{"given":"Xinda","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingze","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9144-4496","authenticated-orcid":false,"given":"Hongwei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6248-8457","authenticated-orcid":false,"given":"Lidan","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.biortech.2026.135460_b0005","doi-asserted-by":"crossref","first-page":"6756","DOI":"10.1073\/pnas.95.12.6756","article-title":"Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-alpha-bisabolene synthase from grand fir (Abies grandis)","volume":"95","author":"Bohlmann","year":"1998","journal-title":"PNAS"},{"key":"10.1016\/j.biortech.2026.135460_b0010","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1186\/s13068-020-01809-6","article-title":"Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of \u03b2-carotene in Saccharomyces cerevisiae","volume":"13","author":"Bu","year":"2020","journal-title":"Biotechnol. Biofuels"},{"issue":"23","key":"10.1016\/j.biortech.2026.135460_b0015","doi-asserted-by":"crossref","first-page":"3943","DOI":"10.1002\/1873-3468.13964","article-title":"Transcription factors and ABC transporters: from pleiotropic drug resistance to cellular signaling in yeast","volume":"594","author":"Buechel","year":"2020","journal-title":"FEBS Lett."},{"issue":"1","key":"10.1016\/j.biortech.2026.135460_b0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pep.2005.03.016","article-title":"SUMO fusion technology for difficult-to-express proteins","volume":"43","author":"Butt","year":"2005","journal-title":"Protein Expr. Purif."},{"issue":"000","key":"10.1016\/j.biortech.2026.135460_b0025","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ymben.2022.11.002","article-title":"Engineering yeast for high-level production of diterpenoid sclareol","volume":"75","author":"Cao","year":"2023","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2026.135460_b0030","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ymben.2023.03.012","article-title":"Metabolic flux enhancement from the translational fusion of terpene synthases is linked to terpene synthase accumulation","volume":"77","author":"Cheah","year":"2023","journal-title":"Metab. Eng."},{"issue":"1","key":"10.1016\/j.biortech.2026.135460_b0035","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202400669","article-title":"Triune Engineering Approach for (+)-valencene overproduction in Yarrowia lipolytica","volume":"20","author":"Chen","year":"2025","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.biortech.2026.135460_b0040","doi-asserted-by":"crossref","first-page":"11152","DOI":"10.1038\/ncomms11152","article-title":"Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways","volume":"7","author":"DeLoache","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135460_b0045","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.biotno.2022.12.005","article-title":"Over-expression of alpha-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox","volume":"4","author":"El-Sharoud","year":"2023","journal-title":"Biotechnol. Notes"},{"issue":"4","key":"10.1016\/j.biortech.2026.135460_b0050","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1021\/acssynbio.4c00728","article-title":"Developing thermosensitive metabolic regulation strategies in the fermentation process of Saccharomyces cerevisiae to enhance alpha-bisabolene production","volume":"14","author":"Feng","year":"2025","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135460_b0055","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/nprot.2007.15","article-title":"Large-scale high-efficiency yeast transformation using the LiAc\/SS carrier DNA\/PEG method","volume":"2","author":"Gietz","year":"2007","journal-title":"Nat. Protoc."},{"issue":"8","key":"10.1016\/j.biortech.2026.135460_b0060","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nchembio.2429","article-title":"Harnessing yeast organelles for metabolic engineering","volume":"13","author":"Hammer","year":"2017","journal-title":"Nat. Chem. Biol."},{"issue":"2","key":"10.1016\/j.biortech.2026.135460_b0065","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1021\/acssynbio.1c00484","article-title":"Secretory production of tocotrienols in Saccharomyces cerevisiae","volume":"11","author":"Jiao","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2026.135460_b0070","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.ymben.2017.11.004","article-title":"Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli","volume":"44","author":"Kim","year":"2017","journal-title":"Metab. Eng."},{"issue":"1","key":"10.1016\/j.biortech.2026.135460_b0075","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1006\/prep.2001.1415","article-title":"Accurate disulfide formation in Escherichia coli: overexpression and characterization of the first domain (HF6478) of the multiple Kazal-type inhibitor LEKTI","volume":"22","author":"Lauber","year":"2001","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.biortech.2026.135460_b0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2015.08.007","article-title":"De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae","volume":"32","author":"Li","year":"2015","journal-title":"Metab. Eng."},{"issue":"30","key":"10.1016\/j.biortech.2026.135460_b0085","doi-asserted-by":"crossref","first-page":"11534","DOI":"10.1021\/acs.jafc.3c02472","article-title":"Combining metabolic engineering and lipid droplet storage engineering for improved alpha-bisabolene production in Yarrowia Lipolytica","volume":"71","author":"Lu","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2026.135460_b0090","doi-asserted-by":"crossref","first-page":"12851","DOI":"10.1038\/ncomms12851","article-title":"Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae","volume":"7","author":"Lv","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135460_b0095","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jbiotec.2014.06.024","article-title":"Enhanced isoprene biosynthesis in Saccharomyces cerevisiae by engineering of the native acetyl-CoA and mevalonic acid pathways with a push-pull-restrain strategy","volume":"186","author":"Lv","year":"2014","journal-title":"J. Biotechnol."},{"issue":"12","key":"10.1016\/j.biortech.2026.135460_b0100","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1016\/j.str.2011.09.013","article-title":"Structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production","volume":"19","author":"McAndrew","year":"2011","journal-title":"Structure"},{"issue":"7622","key":"10.1016\/j.biortech.2026.135460_b0105","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/nature19769","article-title":"Rewriting yeast central carbon metabolism for industrial isoprenoid production","volume":"537","author":"Meadows","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.biortech.2026.135460_b0110","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ymben.2022.10.004","article-title":"Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production","volume":"74","author":"Mukherjee","year":"2022","journal-title":"Metab. Eng."},{"issue":"14","key":"10.1016\/j.biortech.2026.135460_b0115","doi-asserted-by":"crossref","first-page":"9194","DOI":"10.1016\/S0021-9258(18)31570-9","article-title":"Thioredoxin deficiency in yeast prolongs S phase and shortens the G1 interval of the cell cycle","volume":"266","author":"Muller","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.biortech.2026.135460_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.130379","article-title":"Efficient synthesis of squalene by cytoplasmic-peroxisomal engineering and regulating lipid metabolism in Yarrowia lipolytica","volume":"395","author":"Ning","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135460_b0125","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.ymben.2012.07.010","article-title":"Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production","volume":"15","author":"Ozaydin","year":"2013","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2026.135460_b0130","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/ncomms1494","article-title":"Identification and microbial production of a terpene-based advanced biofuel","volume":"2","author":"Peralta-Yahya","year":"2011","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135460_b0135","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ymben.2014.11.007","article-title":"Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae","volume":"28","author":"Xie","year":"2015","journal-title":"Metab. Eng."},{"issue":"1","key":"10.1016\/j.biortech.2026.135460_b0140","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/s41467-023-44654-5","article-title":"De novo biosynthesis of the hops bioactive flavonoid xanthohumol in yeast","volume":"15","author":"Yang","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135460_b0145","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ymben.2016.07.008","article-title":"Mitochondrial acetyl-CoA utilization pathway for terpenoid productions","volume":"38","author":"Yuan","year":"2016","journal-title":"Metab. Eng."},{"key":"10.1016\/j.biortech.2026.135460_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2021.125978","article-title":"Metabolism and strategies for enhanced supply of acetyl-CoA in Saccharomyces cerevisiae","volume":"342","author":"Zhang","year":"2021","journal-title":"Bioresour. Technol."},{"issue":"2","key":"10.1016\/j.biortech.2026.135460_b0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.bidere.2025.100023","article-title":"Enhanced vitamin a production by engineering transporters, ATP and precursor supply","volume":"7","author":"Zhang","year":"2025","journal-title":"Biodesign Res."},{"issue":"20","key":"10.1016\/j.biortech.2026.135460_b0160","doi-asserted-by":"crossref","first-page":"8145","DOI":"10.1039\/D3GC01936E","article-title":"Biosynthesis of \u03b1-bisabolene from low-cost renewable feedstocks by peroxisome engineering and systems metabolic engineering of the yeast Yarrowia lipolytica","volume":"25","author":"Zhao","year":"2023","journal-title":"Green Chem."},{"issue":"6","key":"10.1016\/j.biortech.2026.135460_b0165","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1111\/1751-7915.13768","article-title":"High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica","volume":"14","author":"Zhao","year":"2021","journal-title":"J. Microbial. Biotechnol."},{"issue":"29","key":"10.1016\/j.biortech.2026.135460_b0175","doi-asserted-by":"crossref","first-page":"9644","DOI":"10.1021\/acssuschemeng.2c03063","article-title":"Mitochondrial engineering of Yarrowia lipolytica for sustainable production of \u03b1-bisabolene from waste cooking oil","volume":"10","author":"Zhu","year":"2022","journal-title":"ACS Sustain. Chem. Eng."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015427?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015427?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:54:09Z","timestamp":1790207649000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426015427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":34,"alternative-id":["S0960852426015427"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135460","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient biosynthesis of \u0251-bisabolene in S. cerevisiae by combinatorial regulation of acetyl-CoA supply, bisabolene synthase expression, and bisabolene transportation","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135460","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135460"}}