{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:17:09Z","timestamp":1792613829081,"version":"4.5.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.biortech.2026.135476","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:59:18Z","timestamp":1784645958000},"page":"135476","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Biochar modulates methanotrophic metabolism to enhance methane oxidation and mitigate greenhouse gas emissions"],"prefix":"10.1016","volume":"461","author":[{"given":"Qinqin","family":"Hao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1934-3674","authenticated-orcid":false,"given":"Fanghua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2026.135476_b0005","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1038\/s41467-025-56133-0","article-title":"Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands","volume":"16","author":"Bechtold","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135476_b0010","doi-asserted-by":"crossref","first-page":"11728","DOI":"10.1038\/ncomms11728","article-title":"Conventional methanotrophs are responsible for atmospheric methane oxidation in paddy soils","volume":"7","author":"Cai","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135476_b0015","doi-asserted-by":"crossref","first-page":"5019","DOI":"10.1038\/srep05019","article-title":"Promoting interspecies electron transfer with biochar","volume":"4","author":"Chen","year":"2014","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biortech.2026.135476_b0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.156654","article-title":"Hydrogen bonds between the oxygen-containing functional groups of biochar and organic contaminants significantly enhance sorption affinity","volume":"499","author":"Dong","year":"2024","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135476_b0025","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.soilbio.2011.11.016","article-title":"Mechanisms of biochar decreasing methane emission from Chinese paddy soils","volume":"46","author":"Feng","year":"2012","journal-title":"Soil Biol. Biochem."},{"key":"10.1016\/j.biortech.2026.135476_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.173170","article-title":"Negative impacts of perishable waste biochar to Escherichia coli and exploring potential damage factors","volume":"933","author":"Gao","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135476_b0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.soilbio.2022.108863","article-title":"Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil","volume":"175","author":"Hao","year":"2022","journal-title":"Soil Biol. Biochem."},{"issue":"4","key":"10.1016\/j.biortech.2026.135476_b0040","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/gcbb.12376","article-title":"Effects of biochar application on soil greenhouse gas fluxes: a meta\u2010analysis","volume":"9","author":"He","year":"2016","journal-title":"GCB Bioenergy"},{"issue":"17","key":"10.1016\/j.biortech.2026.135476_b0045","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.ads6093","article-title":"Climate-induced shifts in sulfate dynamics regulate anaerobic methane oxidation in a coastal wetland","volume":"11","author":"Lee","year":"2025","journal-title":"Sci. Adv."},{"issue":"4","key":"10.1016\/j.biortech.2026.135476_b0050","doi-asserted-by":"crossref","DOI":"10.1093\/nsr\/nwaf094","article-title":"Methane and nitrous oxide budget for Chinese natural terrestrial ecosystems","volume":"12","author":"Li","year":"2025","journal-title":"Natl. Sci. Rev."},{"issue":"17","key":"10.1016\/j.biortech.2026.135476_b0055","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1002\/ldr.4405","article-title":"Biochar affects greenhouse gas emissions in various environments: a critical review","volume":"33","author":"Lyu","year":"2022","journal-title":"Land Degrad. Dev."},{"key":"10.1016\/j.biortech.2026.135476_b0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2025.127790","article-title":"Microbial mechanisms of biochar reducing methane emission in a rice-crayfish integrated system","volume":"395","author":"Ma","year":"2025","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.biortech.2026.135476_b0065","doi-asserted-by":"crossref","DOI":"10.1111\/gcb.17127","article-title":"The hidden roots of wetland methane emissions","volume":"30","author":"M\u00e4\u00e4tt\u00e4","year":"2024","journal-title":"Glob. Chang. Biol."},{"key":"10.1016\/j.biortech.2026.135476_b0070","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s42773-021-00096-0","article-title":"Exploring long-term effects of biochar on mitigating methane emissions from paddy soil: a review","volume":"3","author":"Nan","year":"2021","journal-title":"Biochar"},{"issue":"5","key":"10.1016\/j.biortech.2026.135476_b0075","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1128\/AEM.03795-14","article-title":"Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense","volume":"81","author":"Puri","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2026.135476_b0080","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s42773-022-00138-1","article-title":"Biochar applications influence soil physical and chemical properties, microbial diversity, and crop productivity: a meta-analysis","volume":"4","author":"Singh","year":"2022","journal-title":"Biochar"},{"key":"10.1016\/j.biortech.2026.135476_b0085","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00815-18","article-title":"Members of the genus methylobacter are inferred to account for the majority of aerobic methane oxidation in oxic soils from a freshwater wetland","volume":"9","author":"Smith","year":"2018","journal-title":"MBio"},{"key":"10.1016\/j.biortech.2026.135476_b0090","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1515\/pac-2014-1117","article-title":"Physisorption of gases, with special reference to the evaluation of surface area and pore size distribution (IUPAC Technical Report)","volume":"87","author":"Thommes","year":"2015","journal-title":"Pure Appl. Chem."},{"key":"10.1016\/j.biortech.2026.135476_b0095","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s11157-020-09523-3","article-title":"Biochar physicochemical properties: pyrolysis temperature and feedstock kind effects","volume":"19","author":"Tomczyk","year":"2020","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"10.1016\/j.biortech.2026.135476_b0100","doi-asserted-by":"crossref","first-page":"6315","DOI":"10.1038\/s41467-022-34007-z","article-title":"Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum","volume":"13","author":"Vitt","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.biortech.2026.135476_b0105","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s42773-025-00478-8","article-title":"Biochar conductivity enhances methane generation in paddy soil by facilitating electron transfer mediated by dissolved organic matter","volume":"7","author":"Wu","year":"2025","journal-title":"Biochar"},{"key":"10.1016\/j.biortech.2026.135476_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.geoderma.2023.116498","article-title":"Biochar single application and reapplication decreased soil greenhouse gas and nitrogen oxide emissions from rice\u2013wheat rotation: a three-year field observation","volume":"435","author":"Wu","year":"2023","journal-title":"Geoderma"},{"key":"10.1016\/j.biortech.2026.135476_b0115","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.envpol.2019.07.073","article-title":"Biochar can mitigate methane emissions by improving methanotrophs for prolonged period in fertilized paddy soils","volume":"253","author":"Wu","year":"2019","journal-title":"Environ. Pollut."},{"issue":"27","key":"10.1016\/j.biortech.2026.135476_b0120","doi-asserted-by":"crossref","first-page":"13551","DOI":"10.1021\/acs.est.5c00581","article-title":"Distribution hotspots, formation mechanisms, and ecological effects of reactive oxygen species in soil and sediment: a critical review","volume":"59","author":"Yao","year":"2025","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.biortech.2026.135476_b0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2021.132313","article-title":"Biochar as a novel carbon-negative electron source and mediator: electron exchange capacity (EEC) and environmentally persistent free radicals (EPFRs): a review","volume":"429","author":"Yuan","year":"2022","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135476_b0130","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.biortech.2017.06.154","article-title":"Applications of biochar in redox-mediated reactions","volume":"246","author":"Yuan","year":"2017","journal-title":"Bioresour. Technol."},{"issue":"23","key":"10.1016\/j.biortech.2026.135476_b0135","doi-asserted-by":"crossref","first-page":"6832","DOI":"10.1128\/JB.183.23.6832-6840.2001","article-title":"Membrane-associated quinoprotein formaldehyde dehydrogenase from Methylococcus capsulatus Bath","volume":"183","author":"Zahn","year":"2001","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.biortech.2026.135476_b0140","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.watres.2018.05.058","article-title":"Surface properties of activated sludge-derived biochar determine the facilitating effects on Geobacter co-cultures","volume":"142","author":"Zhang","year":"2018","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135476_b0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.122318","article-title":"Effect of pyrolysis temperature and correlation analysis on the yield and physicochemical properties of crop residue biochar","volume":"296","author":"Zhang","year":"2020","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135476_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.130990","article-title":"Biochar promotes microbial CO2 fixation by regulating feedback inhibition of metabolites","volume":"406","author":"Zhao","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135476_b0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.geoderma.2024.116994","article-title":"Biochar-based urea increases soil methane uptake in a subtropical forest","volume":"449","author":"Zhou","year":"2024","journal-title":"Geoderma"}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015580?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015580?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:01:51Z","timestamp":1790208111000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426015580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":31,"alternative-id":["S0960852426015580"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135476","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biochar modulates methanotrophic metabolism to enhance methane oxidation and mitigate greenhouse gas emissions","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135476","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135476"}}