{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:59:27Z","timestamp":1790211567716,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22478199"],"award-info":[{"award-number":["22478199"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013085","name":"Program for Jiangsu Excellent Scientific and Technological Innovation Team","doi-asserted-by":"publisher","award":["KYCX24_1860"],"award-info":[{"award-number":["KYCX24_1860"]}],"id":[{"id":"10.13039\/501100013085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2024YFA0917800"],"award-info":[{"award-number":["2024YFA0917800"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Jiangsu Province Natural Science Foundation","doi-asserted-by":"publisher","award":["BK20250141"],"award-info":[{"award-number":["BK20250141"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012154","name":"Graduate Research and Innovation Projects of Jiangsu Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012154","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.biortech.2026.135492","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:28:32Z","timestamp":1784820512000},"page":"135492","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Overcoming catalytic barriers to reprogram acyltransferases for bis(2-hydroxyethyl) terephthalate hydrolysis"],"prefix":"10.1016","volume":"461","author":[{"given":"Jie","family":"Qiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibo","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyao","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7642-721X","authenticated-orcid":false,"given":"Xiujuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2026.135492_b0005","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"issue":"3","key":"10.1016\/j.biortech.2026.135492_b0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jece.2024.112507","article-title":"Chemical recycling of polyethylene terephthalate: a mini-review","volume":"12","author":"Babaei","year":"2024","journal-title":"J. Environ. Chem. Eng."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0015","article-title":"Promiscuous acyltransferases for ester and amide synthesis in aqueous solution","volume":"442","author":"Baumert","year":"2024","journal-title":"Catal. Today"},{"issue":"15","key":"10.1016\/j.biortech.2026.135492_b0020","article-title":"Highly efficient kilogram-scale mechanochemical catalytic depolymerization of PET polyester waste into reusable monomers","volume":"500","author":"Cai","year":"2024","journal-title":"Chem. Eng. J."},{"issue":"5","key":"10.1016\/j.biortech.2026.135492_b0025","article-title":"Directional-path modification strategy enhances PET hydrolase catalysis of plastic degradation","volume":"433","author":"Chen","year":"2022","journal-title":"J. Hazard. Mater."},{"issue":"9","key":"10.1016\/j.biortech.2026.135492_b0030","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1039\/D1GC00763G","article-title":"CompassR-guided recombination unlocks design principles to stabilize lipases in ILs with minimal experimental efforts","volume":"23","author":"Cui","year":"2021","journal-title":"Green Chem."},{"issue":"3","key":"10.1016\/j.biortech.2026.135492_b0035","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.tibtech.2024.10.018","article-title":"Producing multiple chemicals through biological upcycling of waste poly(ethylene terephthalate)","volume":"43","author":"Diao","year":"2025","journal-title":"Trends Biotechnol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0040","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/pro.3934","article-title":"The AutoDock suite at 30","volume":"30","author":"Goodsell","year":"2021","journal-title":"Protein Sci."},{"key":"10.1016\/j.biortech.2026.135492_b0045","doi-asserted-by":"crossref","first-page":"580","DOI":"10.3390\/catal16070580","article-title":"Enzymatic degradation of crystalline polyethylene terephthalate: challenges, strategies, and perspectives towards sustainable recycling","volume":"16","author":"Graefe","year":"2026","journal-title":"Catalysts"},{"issue":"3","key":"10.1016\/j.biortech.2026.135492_b0050","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1007\/s10311-024-01714-6","article-title":"Natural and engineered enzymes for polyester degradation: a review","volume":"22","author":"Guo","year":"2024","journal-title":"Environ. Chem. Lett."},{"issue":"16","key":"10.1016\/j.biortech.2026.135492_b0055","doi-asserted-by":"crossref","first-page":"8027","DOI":"10.1021\/acs.est.5c00639","article-title":"Biodegradation of PET by deep-sea Pseudomonas chengduensis BC1815 through utilization of ethylene glycol monomer for growth","volume":"59","author":"Guo","year":"2025","journal-title":"Environ. Sci. Technol."},{"issue":"5","key":"10.1016\/j.biortech.2026.135492_b0060","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1021\/ct100747y","article-title":"Validation of the GROMOS 54A7 force field with respect to \u03b2-peptide folding","volume":"7","author":"Huang","year":"2011","journal-title":"J. Chem. Theory Comput."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0065","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"J. Mol. Graph."},{"issue":"7903","key":"10.1016\/j.biortech.2026.135492_b0070","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/s41586-021-04350-0","article-title":"Critical advances and future opportunities in upcycling commodity polymers","volume":"603","author":"Jehanno","year":"2022","journal-title":"Nature"},{"issue":"7873","key":"10.1016\/j.biortech.2026.135492_b0075","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"20","key":"10.1016\/j.biortech.2026.135492_b0080","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.1093\/bioinformatics\/bty386","article-title":"CAVER Analyst 2.0: analysis and visualization of channels and tunnels in protein structures and molecular dynamics trajectories","volume":"34","author":"Jurcik","year":"2018","journal-title":"Bioinformatics"},{"issue":"7","key":"10.1016\/j.biortech.2026.135492_b0085","doi-asserted-by":"crossref","first-page":"3996","DOI":"10.1021\/acscatal.0c04014","article-title":"One-pot chemo-bioprocess of PET depolymerization and recycling enabled by a biocompatible catalyst","volume":"11","author":"Kim","year":"2021","journal-title":"Betaine. Acs Catal."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0090","doi-asserted-by":"crossref","first-page":"17892","DOI":"10.1038\/s41598-024-60824-x","article-title":"Computational studies on the catalytic potential of the double active site for enzyme engineering","volume":"14","author":"Krishna","year":"2024","journal-title":"Sci. Rep."},{"issue":"46","key":"10.1016\/j.biortech.2026.135492_b0095","doi-asserted-by":"crossref","first-page":"19873","DOI":"10.1021\/acs.iecr.3c03422","article-title":"Chemical recycling development of poly(ethylene terephthalate) by glycolysis and cooling crystallization with water","volume":"62","author":"Lee","year":"2023","journal-title":"Ind. Eng. Chem. Res."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0100","doi-asserted-by":"crossref","first-page":"4169","DOI":"10.1038\/s41467-023-39929-w","article-title":"Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling","volume":"14","author":"Li","year":"2023","journal-title":"Nat. Commun."},{"issue":"13","key":"10.1016\/j.biortech.2026.135492_b0105","doi-asserted-by":"crossref","DOI":"10.1002\/cssc.202301612","article-title":"Unlocking a sustainable future for plastics: a chemical-enzymatic pathway for efficient conversion of mixed waste to MHET and energy-saving PET recycling","volume":"17","author":"Li","year":"2024","journal-title":"ChemSusChem"},{"issue":"19","key":"10.1016\/j.biortech.2026.135492_b0110","doi-asserted-by":"crossref","first-page":"10995","DOI":"10.1021\/acs.jafc.3c08365","article-title":"Computer-aided semi-rational design to enhance the activity of l-sorbosone dehydrogenase from gluconobacter oxidans WSH-004","volume":"72","author":"Li","year":"2024","journal-title":"J. Agric. Food Chem."},{"issue":"7","key":"10.1016\/j.biortech.2026.135492_b0115","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1021\/cbe.5c00013","article-title":"Biocomposites of enzymes and covalent organic frameworks: a novel family of heterogenous biocatalysis","volume":"2","author":"Li","year":"2025","journal-title":"Chem Bio Eng."},{"key":"10.1016\/j.biortech.2026.135492_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.engmic.2021.100003","article-title":"Potential one-step strategy for PET degradation and PHB biosynthesis through co-cultivation of two engineered microorganisms","volume":"1","author":"Liu","year":"2021","journal-title":"Eng. Microbiol."},{"issue":"5","key":"10.1016\/j.biortech.2026.135492_b0125","article-title":"Structure-guided discovery and rational design of a new poly(ethylene terephthalate) hydrolase from AlphaFold protein structure database","volume":"480","author":"Liu","year":"2024","journal-title":"J. Hazard. Mater."},{"issue":"10","key":"10.1016\/j.biortech.2026.135492_b0130","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.1039\/D0GC00327A","article-title":"Degradation of poly(ethylene terephthalate) catalyzed by metal-free choline-based ionic liquids","volume":"22","author":"Liu","year":"2020","journal-title":"Green Chem."},{"key":"10.1016\/j.biortech.2026.135492_b0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.127800","article-title":"Structure-oriented engineering of nitrile hydratase: reshaping of substrate access tunnel and binding pocket for efficient synthesis of cinnamamide","volume":"254","author":"Ma","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.biortech.2026.135492_b0140","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.biochi.2020.09.013","article-title":"MetA (Rv3341) from H37Rv strain exhibits substrate dependent dual role of transferase and hydrolase activity","volume":"179","author":"Maurya","year":"2020","journal-title":"Biochimie"},{"issue":"28","key":"10.1016\/j.biortech.2026.135492_b0145","doi-asserted-by":"crossref","first-page":"11607","DOI":"10.1002\/anie.202003635","article-title":"Sequence-based prediction of promiscuous acyltransferase activity in hydrolases","volume":"59","author":"M\u00fcller","year":"2020","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.biortech.2026.135492_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2024.109328","article-title":"Integrating glycolysis and enzymatic catalyst to convert waste poly (ethylene terephthalate) into terephthalic acid","volume":"207","author":"Pan","year":"2024","journal-title":"Biochem. Eng. J."},{"issue":"12","key":"10.1016\/j.biortech.2026.135492_b0155","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202300320","article-title":"Evolving robust and interpretable enzymes for the bioethanol industry","volume":"62","author":"Qiao","year":"2023","journal-title":"Angew. Chem. Int. Ed. Engl."},{"issue":"5","key":"10.1016\/j.biortech.2026.135492_b0160","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1038\/s41893-026-01785-z","article-title":"Microbial upcycling of plastic waste to levodopa","volume":"9","author":"Royer","year":"2026","journal-title":"Nat. Sustainability"},{"issue":"16","key":"10.1016\/j.biortech.2026.135492_b0165","doi-asserted-by":"crossref","first-page":"9132","DOI":"10.1039\/D3GC04933G","article-title":"Harnessing solvation-guided engineering to enhance deep eutectic solvent resistance and thermostability in enzymes","volume":"26","author":"Sheng","year":"2024","journal-title":"Green Chem."},{"issue":"2","key":"10.1016\/j.biortech.2026.135492_b0170","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1021\/acscatal.4c06344","article-title":"Advances and challenges for catalytic recycling and upgrading of real-world mixed plastic waste","volume":"15","author":"Shi","year":"2024","journal-title":"ACS Catal."},{"issue":"10","key":"10.1016\/j.biortech.2026.135492_b0175","article-title":"Ancestral sequence reconstruction and comprehensive computational simulations unmask an efficient PET hydrolase with the wobbled catalytic triad","volume":"18","author":"Song","year":"2025","journal-title":"ChemSusChem"},{"issue":"8","key":"10.1016\/j.biortech.2026.135492_b0180","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s10822-023-00511-7","article-title":"OFraMP: a fragment-based tool to facilitate the parametrization of large molecules","volume":"37","author":"Stroet","year":"2023","journal-title":"J. Comput. Aid. Mol. Des."},{"issue":"W1","key":"10.1016\/j.biortech.2026.135492_b0185","doi-asserted-by":"crossref","first-page":"W314","DOI":"10.1093\/nar\/gkt503","article-title":"Depth: a web server to compute depth, cavity sizes, detect potential small-molecule ligand-binding cavities and predict the pK of ionizable residues in proteins","volume":"41","author":"Tan","year":"2013","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"10.1016\/j.biortech.2026.135492_b0190","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1080\/07388551.2023.2170861","article-title":"Enhancing plastic biodegradation process: strategies and opportunities","volume":"44","author":"Thew","year":"2024","journal-title":"Crit. Rev. Biotechnol."},{"issue":"23","key":"10.1016\/j.biortech.2026.135492_b0195","doi-asserted-by":"crossref","first-page":"4986","DOI":"10.1093\/bioinformatics\/btz386","article-title":"CaverDock: a molecular docking-based tool to analyse ligand transport through protein tunnels and channels","volume":"35","author":"Vavra","year":"2019","journal-title":"Bioinformatics"},{"key":"10.1016\/j.biortech.2026.135492_b0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.chphi.2024.100481","article-title":"Solvation effects, structural, vibrational analysis, chemical reactivity, nanocages, ELF, LOL, docking and MD simulation on Sitagliptin","volume":"8","author":"Venkatesh","year":"2024","journal-title":"Chem. Phys. Impact"},{"issue":"20","key":"10.1016\/j.biortech.2026.135492_b0205","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.ads8318","article-title":"Ancestral reconstruction of polyethylene terephthalate degrading cutinases reveals a rugged and unexplored sequence-fitness landscape","volume":"11","author":"Vongsouthi","year":"2025","journal-title":"Sci. Adv."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0210","doi-asserted-by":"crossref","first-page":"4795","DOI":"10.1038\/s41467-023-40455-y","article-title":"Catalytic site flexibility facilitates the substrate and catalytic promiscuity of dual lipase\/transferase","volume":"14","author":"Wang","year":"2023","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.biortech.2026.135492_b0215","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202315125","article-title":"Corner engineering: tailoring enzymes for enhanced resistance and thermostability in deep eutectic solvents","volume":"63","author":"Wang","year":"2024","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.biortech.2026.135492_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.eurpolymj.2023.112578","article-title":"Synergistic catalysis of ionic liquids and metal salts for facile PET glycolysis","volume":"201","author":"Wang","year":"2023","journal-title":"Eur. Polym. J."},{"key":"10.1016\/j.biortech.2026.135492_b0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2021.113267","article-title":"Progress in the catalytic glycolysis of polyethylene terephthalate","volume":"296","author":"Xin","year":"2021","journal-title":"J. Environ. Manage."},{"issue":"1","key":"10.1016\/j.biortech.2026.135492_b0230","article-title":"Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning","volume":"275","author":"Xu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.biortech.2026.135492_b0235","doi-asserted-by":"crossref","DOI":"10.1016\/j.fuel.2024.133858","article-title":"Catalytic depolymerization of poly(ethylene terephthalate) plastic into value-added monomers using metal-modified mesoporous silica","volume":"383","author":"Yang","year":"2025","journal-title":"Fuel"},{"issue":"16","key":"10.1016\/j.biortech.2026.135492_b0240","doi-asserted-by":"crossref","first-page":"16916","DOI":"10.1021\/jacs.6c00776","article-title":"Synergistic barium-oxygen vacancy catalysis engineered with MWW zeolites for efficient PET glycolytic depolymerization","volume":"148","author":"Zhang","year":"2026","journal-title":"J. Am. Chem. Soc."},{"issue":"4","key":"10.1016\/j.biortech.2026.135492_b0245","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.trechm.2025.02.005","article-title":"Interfacial catalysis in enzymatic PET plastic depolymerization","volume":"7","author":"Zhou","year":"2025","journal-title":"Trends Chem."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015749?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426015749?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:58Z","timestamp":1790208058000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426015749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":49,"alternative-id":["S0960852426015749"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135492","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Overcoming catalytic barriers to reprogram acyltransferases for bis(2-hydroxyethyl) terephthalate hydrolysis","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135492","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135492"}}