{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:55:15Z","timestamp":1790902515999,"version":"4.1.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003213","name":"Beijing Municipal Education Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003213","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002888","name":"Beijing Municipal Commission of Education","doi-asserted-by":"publisher","award":["Z161100004516015"],"award-info":[{"award-number":["Z161100004516015"]}],"id":[{"id":"10.13039\/501100002888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.biortech.2026.135602","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T23:46:49Z","timestamp":1786492009000},"page":"135602","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Performance stability and adaptability of embedded phosphorus removal biofillers: insights from microbial community responses and regulatory mechanisms"],"prefix":"10.1016","volume":"462","author":[{"given":"Wei","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyuan","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2026.135602_b0005","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.cej.2016.11.073","article-title":"New insights in the metabolic behaviour of PAO under negligible poly-P reserves","volume":"311","author":"Acevedo","year":"2017","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135602_b0010","unstructured":"APHA, 2005. Standard Methods for the Examination of Water and Wastewater. American Public Health Association. 21th, American Public Health Association, Washington,DC."},{"key":"10.1016\/j.biortech.2026.135602_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.jwpe.2024.105356","article-title":"Influence of operation sequences on phosphorus recovery by polyphosphate-accumulating organisms biofilm: Performance, kinetics and metabolic response","volume":"61","author":"Bi","year":"2024","journal-title":"J. Water Process Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecolind.2020.107219","article-title":"Evaluating spatial characteristics and influential factors of industrial wastewater discharge in China: a spatial econometric approach","volume":"121","author":"Bu","year":"2021","journal-title":"Ecol. Ind."},{"key":"10.1016\/j.biortech.2026.135602_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.165416","article-title":"Phosphorus recovery from synthetic anaerobic fermentation supernatant via vivianite crystallization: Coupling effects of various physicochemical process parameters","volume":"897","author":"Cao","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.148110","article-title":"Disclosing the future food security risk of China based on crop production and water scarcity under diverse socioeconomic and climate scenarios","volume":"790","author":"Chen","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.jwpe.2022.102979","article-title":"Phosphorus absorption and release in biofilm sequencing batch reactor: the combined action of cells and extracellular polymeric substances and the characteristics of polymer metabolism","volume":"49","author":"Chen","year":"2022","journal-title":"J. Water Process Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0040","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.spc.2023.08.015","article-title":"Sustainable development of phosphorus recovery: from a product perspective","volume":"41","author":"Cheng","year":"2023","journal-title":"Sustainable Prod. Consumption"},{"key":"10.1016\/j.biortech.2026.135602_b0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.122546","article-title":"Enhanced selective enrichment of partial nitritation and anammox bacteria in a novel two-stage continuous flow system using flat-type poly (vinylalcohol) cryogel films","volume":"300","author":"Choi","year":"2020","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135602_b0050","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1021\/acssuschemeng.7b03155","article-title":"Phosphate recovery from human waste via the formation of hydroxyapatite during electrochemical wastewater treatment","volume":"6","author":"Cid","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2020.111561","article-title":"Nitrous oxide emission during denitrifying phosphorus removal process: a review on the mechanisms and influencing factors","volume":"278","author":"Dai","year":"2021","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.biortech.2026.135602_b0060","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1016\/j.watres.2007.01.045","article-title":"Anaerobic metabolism of Defluviicoccus vanus related glycogen accumulating organisms (GAOs) with acetate and propionate as carbon sources","volume":"41","author":"Dai","year":"2007","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.138685","article-title":"Phosphorus recovery from wastewater via calcium phosphate precipitation: a critical review of methods, progress, and insights","volume":"330","author":"Deng","year":"2023","journal-title":"Chemosphere"},{"key":"10.1016\/j.biortech.2026.135602_b0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.jece.2025.117812","article-title":"Simultaneous removal and efficient enrichment of low-concentration phosphate in municipal sewage by biofilm process","volume":"13","author":"Ding","year":"2025","journal-title":"J. Environ. Chem. Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.jwpe.2026.109526","article-title":"Technologies and current applications of phosphorus recovery from wastewater treatment surplus sludge","volume":"82","author":"Guo","year":"2026","journal-title":"J. Water Process Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2022.137225","article-title":"Metagenomic analysis of denitrifying phosphorus removal in SBR system: comparison of nitrate and nitrite as electron acceptors","volume":"446","author":"Hou","year":"2022","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135602_b0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.130262","article-title":"High efficiency and stable partial nitration achieved via gel immobilization","volume":"394","author":"Hu","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135602_b0090","doi-asserted-by":"crossref","first-page":"8264","DOI":"10.1021\/bi201284u","article-title":"The original Michaelis constant: translation of the 1913 Michaelis\u2013Menten Paper","volume":"50","author":"Johnson","year":"2011","journal-title":"Biochemistry"},{"key":"10.1016\/j.biortech.2026.135602_b0095","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2025.125786","article-title":"Advancements and feasibility of synergistic approaches in phosphorus recovery from wastewater: a critical review","volume":"386","author":"Kaur","year":"2025","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.biortech.2026.135602_b0100","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1016\/j.watres.2013.01.027","article-title":"Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor","volume":"47","author":"Kodera","year":"2013","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0105","doi-asserted-by":"crossref","first-page":"7032","DOI":"10.1016\/j.watres.2013.08.042","article-title":"Metabolic versatility in full-scale wastewater treatment plants performing enhanced biological phosphorus removal","volume":"47","author":"Lanham","year":"2013","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0110","doi-asserted-by":"crossref","first-page":"28407","DOI":"10.1007\/s11356-023-25388-9","article-title":"Mining phosphorus from waste streams at wastewater treatment plants: a review of enrichment, extraction, and crystallization methods","volume":"30","author":"Li","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.biortech.2026.135602_b0115","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.chemosphere.2006.05.004","article-title":"Effect of initial pH control on enhanced biological phosphorus removal from wastewater containing acetic and propionic acids","volume":"66","author":"Liu","year":"2007","journal-title":"Chemosphere"},{"key":"10.1016\/j.biortech.2026.135602_b0120","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1016\/j.watres.2012.05.058","article-title":"Structural, physicochemical and microbial properties of flocs and biofilms in integrated fixed-film activated sludge (IFFAS) systems","volume":"46","author":"Mahendran","year":"2012","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0125","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1007\/s00253-021-11113-4","article-title":"Metagenomic insights into the effect of sulfate on enhanced biological phosphorus removal","volume":"105","author":"Matsuura","year":"2021","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2026.135602_b0130","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1016\/S0043-1354(98)00129-8","article-title":"Microbiology and biochemistry of the enhanced biological phosphate removal process","volume":"32","author":"Mino","year":"1998","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.127603","article-title":"Metagenomics reveals the metabolism of polyphosphate-accumulating organisms in biofilm sequencing batch reactor: a new model","volume":"360","author":"Ni","year":"2022","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135602_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2023.118114","article-title":"A new insight in enhancing phosphate enrichment in biofilm process: Comparison of the key metabolic pathways in highly-efficient and dominant PAOs based on metagenomics","volume":"341","author":"Ni","year":"2023","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.biortech.2026.135602_b0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2023.117237","article-title":"Metagenomics, metatranscriptomics, and proteomics reveal the metabolic mechanism of biofilm sequencing batch reactor with higher phosphate enrichment capacity under low phosphorus load","volume":"238","author":"Ni","year":"2023","journal-title":"Environ. Res."},{"key":"10.1016\/j.biortech.2026.135602_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.169641","article-title":"A comprehensive investigation to the fate of phosphorus in full-scale wastewater treatment plants using aluminum salts for enhanced phosphorus removal","volume":"913","author":"Ping","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0155","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.cej.2018.02.074","article-title":"Re-visiting the Phostrip process to recover phosphorus from municipal wastewater","volume":"343","author":"Salehi","year":"2018","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135602_b0160","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.2166\/wst.2009.045","article-title":"Phosphorus recovery from wastewater: needs, technologies and costs","volume":"59","author":"Schaum","year":"2009","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.biortech.2026.135602_b0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.seps.2020.100956","article-title":"Assessment and improvement analysis of economic production, water pollution, and sewage treatment efficiency in China","volume":"74","author":"Shi","year":"2021","journal-title":"Socioecon. Plann. Sci."},{"key":"10.1016\/j.biortech.2026.135602_b0170","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s13280-021-01562-6","article-title":"Towards circular phosphorus: the need of inter- and transdisciplinary research to close the broken cycle","volume":"51","author":"Stamm","year":"2021","journal-title":"Ambio"},{"key":"10.1016\/j.biortech.2026.135602_b0175","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/A:1000150523030","article-title":"Metabolism of micro-organisms responsible for enhanced biological phosphorus removal from wastewater, use of dynamic enrichment cultures","volume":"71","author":"van Loosdrecht","year":"1997","journal-title":"Antonie Van Leeuwenhoek"},{"key":"10.1016\/j.biortech.2026.135602_b0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131363","article-title":"Formation mechanism of high biofilm phosphorus storage capacity and its effect on phosphorus uptake-release and carbon source consumption","volume":"412","author":"Wang","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.biortech.2026.135602_b0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.145136","article-title":"Research progress on enhancing the performance of autotrophic nitrogen removal systems using microbial immobilization technology","volume":"774","author":"Wang","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2023.147159","article-title":"Effect of HRT on nitrogen removal from low carbon source wastewater enhanced by slurry and its mechanism","volume":"477","author":"Wu","year":"2023","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.biortech.2026.135602_b0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.jece.2025.119395","article-title":"Simultaneous enhancement of phosphorus release of cell and EPS by carbon source to improve biofilm phosphorus recovery concentration: Performance and mechanism","volume":"13","author":"Wu","year":"2025","journal-title":"J. Environ. Chem. Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0200","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.jes.2021.05.017","article-title":"A new strategy for obtaining highly concentrated phosphorus recovery solution in biofilm phosphorus recovery process","volume":"112","author":"Yang","year":"2022","journal-title":"J. Environ. Sci."},{"key":"10.1016\/j.biortech.2026.135602_b0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.166811","article-title":"Clarification of the phosphorus release mechanism for recovering phosphorus from biofilm sludge in alternating aerobic\/anaerobic biofilm system","volume":"904","author":"Zhang","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0210","article-title":"Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration","volume":"12","author":"Zhang","year":"2024","journal-title":"J. Environ. Chem. Eng."},{"key":"10.1016\/j.biortech.2026.135602_b0215","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.watres.2018.10.061","article-title":"Elucidating functional microorganisms and metabolic mechanisms in a novel engineered ecosystem integrating C, N, P and S biotransformation by metagenomics","volume":"148","author":"Zhang","year":"2019","journal-title":"Water Res."},{"key":"10.1016\/j.biortech.2026.135602_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2025.121204","article-title":"Study on the mechanisms of efficient phosphorus recovery by a pilot-scale biofilm sequencing batch reactor under low carbon demand","volume":"273","author":"Zhang","year":"2025","journal-title":"Environ. Res."},{"key":"10.1016\/j.biortech.2026.135602_b0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.172408","article-title":"Cultivation of phosphate-accumulating biofilm: Study of the effects of acyl-homoserine lactones (AHLs) and cyclic dimeric guanosine monophosphate (c-di-GMP) on the formation of biofilm and the enhancement of phosphate metabolism capacity","volume":"928","author":"Zhao","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.135602_b0230","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2024.118985","article-title":"Phosphate recovery from urban sewage by the biofilm sequencing batch reactor process: Key factors in biofilm formation and related mechanisms","volume":"252","author":"Zheng","year":"2024","journal-title":"Environ. Res."},{"key":"10.1016\/j.biortech.2026.135602_b0235","article-title":"Reevaluating glycogen-accumulating organisms as ecological flexors: mechanistic insights into GAO-mediated enhancement of phosphorus enrichment","author":"Zhu","year":"2025","journal-title":"J. Environ. Sci."},{"key":"10.1016\/j.biortech.2026.135602_b0240","doi-asserted-by":"crossref","DOI":"10.1016\/j.ceja.2026.101352","article-title":"Separation and beneficiation of low-grade phosphate rock: a comprehensive review","volume":"27","author":"Zhu","year":"2026","journal-title":"Chem. Eng. J. Adv."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426016846?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426016846?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:23:29Z","timestamp":1790900609000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426016846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":48,"alternative-id":["S0960852426016846"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135602","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Performance stability and adaptability of embedded phosphorus removal biofillers: insights from microbial community responses and regulatory mechanisms","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135602","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135602"}}