{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T14:31:22Z","timestamp":1793802682025,"version":"4.6.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470058"],"award-info":[{"award-number":["32470058"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072201"],"award-info":[{"award-number":["32072201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372286"],"award-info":[{"award-number":["32372286"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515010835"],"award-info":[{"award-number":["2025A1515010835"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515011967"],"award-info":[{"award-number":["2023A1515011967"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515012223"],"award-info":[{"award-number":["2023A1515012223"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.biortech.2026.135691","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T15:16:14Z","timestamp":1787411774000},"page":"135691","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Identification of chloroplast transit peptides inDunaliella salina and their application for heterologous astaxanthin production"],"prefix":"10.1016","volume":"463","author":[{"given":"Jv-Liang","family":"Dai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Li","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Hua","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Guo","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.biortech.2026.135691_b0005","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1007\/s11274-014-1714-0","article-title":"The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins","volume":"30","author":"Akbari","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"issue":"3","key":"10.1016\/j.biortech.2026.135691_b0010","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s11120-015-0188-8","article-title":"Metabolic engineering of Dunaliella salina for production of ketocarotenoids","volume":"127","author":"Anila","year":"2016","journal-title":"Photosynth. Res."},{"issue":"3","key":"10.1016\/j.biortech.2026.135691_b0015","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.jmb.2010.07.052","article-title":"Chloroplast import signals: the length requirement for translocation In Vitro and In Vivo","volume":"402","author":"Bionda","year":"2010","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0020","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-024-02599-4","article-title":"Genetic engineering of Nannochloropsis oceanica to produce canthaxanthin and ketocarotenoids","volume":"23","author":"Canini","year":"2024","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.biortech.2026.135691_b0025","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2022.825797","article-title":"Transit peptides often require downstream unstructured sequence for efficient chloroplast import in Chlamydomonas reinhardtii","volume":"13","author":"Caspari","year":"2022","journal-title":"Front. Plant Sci."},{"issue":"2","key":"10.1016\/j.biortech.2026.135691_b0030","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms10020361","article-title":"Transformation of Dunaliella salina by Agrobacterium tumefaciens for the expression of the hemagglutinin of avian influenza virus H5","volume":"10","author":"Castellanos-Huerta","year":"2022","journal-title":"Microorganisms"},{"issue":"2","key":"10.1016\/j.biortech.2026.135691_b0035","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1002\/jcp.30647","article-title":"The expression pattern of \u03b2-carotene ketolase gene restricts the accumulation of astaxanthin in Dunaliella under salt stress","volume":"237","author":"Chen","year":"2022","journal-title":"J. Cell. Physiology"},{"key":"10.1016\/j.biortech.2026.135691_b0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.plaphy.2024.108697","article-title":"Enhancing astaxanthin accumulation through the expression of the plant-derived astaxanthin biosynthetic pathway in Dunaliella salina","volume":"211","author":"Chen","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.biortech.2026.135691_b0045","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.sajb.2020.07.008","article-title":"Astaxanthin biosynthesis in transgenic Dunaliella salina (Chlorophyceae) enhanced tolerance to high irradiation stress","volume":"133","author":"Chen","year":"2020","journal-title":"S. Afr. J. Bot."},{"issue":"9","key":"10.1016\/j.biortech.2026.135691_b0050","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1021\/acssynbio.8b00251","article-title":"Birth of a photosynthetic chassis: a MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii","volume":"7","author":"Crozet","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.biortech.2026.135691_b0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2025.133597","article-title":"Divergent roles of three glycerol-3-phosphate dehydrogenase isoforms in glycerol and lipid metabolism of Dunaliella salina and their implications for biodiesel production","volume":"441","author":"Dai","year":"2026","journal-title":"Bioresour. Technol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0060","doi-asserted-by":"crossref","DOI":"10.1104\/pp.114.248450","article-title":"Proteome analysis of cytoplasmatic and plastidic \u03b2-xarotene lipid droplets in Dunaliella bardawil","volume":"167","author":"Davidi","year":"2015","journal-title":"Plant Physiol."},{"issue":"10","key":"10.1016\/j.biortech.2026.135691_b0065","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1007\/s00253-014-5636-4","article-title":"Dunaliella salina as a novel host for the production of recombinant proteins","volume":"98","author":"Feng","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0070","doi-asserted-by":"crossref","DOI":"10.1007\/s00299-024-03409-2","article-title":"Targeting signals required for protein sorting to sub-chloroplast compartments","volume":"44","author":"Kang","year":"2024","journal-title":"Plant Cell Rep."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0075","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/pce.14724","article-title":"Deciphering the \u03b2-carotene hyperaccumulation in Dunaliella by the comprehensive analysis of Dunaliella salina and Dunaliella tertiolecta under high light conditions","volume":"47","author":"Kim","year":"2024","journal-title":"Plant Cell Environ."},{"issue":"12","key":"10.1016\/j.biortech.2026.135691_b0080","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.tplants.2014.09.004","article-title":"Shredding the signal: targeting peptide degradation in mitochondria and chloroplasts","volume":"19","author":"Kmiec","year":"2014","journal-title":"Trends Plant Sci."},{"issue":"8","key":"10.1016\/j.biortech.2026.135691_b0085","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1007\/s00253-014-6354-7","article-title":"Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit","volume":"99","author":"Lauersen","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2026.135691_b0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.algal.2025.104248","article-title":"Ecophysiological modeling of the impact of light intensity and quality on microalgal growth in outdoor high-density open ponds","volume":"91","author":"Le Noac'h","year":"2025","journal-title":"Algal Res."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0095","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1104\/pp.17.01553","article-title":"Prolines in transit peptides are crucial for efficient preprotein translocation into chloroplasts","volume":"176","author":"Lee","year":"2018","journal-title":"Plant Physiol."},{"issue":"2","key":"10.1016\/j.biortech.2026.135691_b0100","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1007\/s11033-009-9901-z","article-title":"A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina","volume":"37","author":"Li","year":"2010","journal-title":"Mol. Biol. Rep."},{"issue":"2","key":"10.1016\/j.biortech.2026.135691_b0105","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s10811-007-9197-0","article-title":"Inducible EGFP expression under the control of the nitrate reductase gene promoter in transgenic Dunaliella salina","volume":"20","author":"Li","year":"2008","journal-title":"J. Appl. Phycol."},{"issue":"2","key":"10.1016\/j.biortech.2026.135691_b0110","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1080\/10425170701447614","article-title":"Identification of a gene encoding the light-harvesting chlorophyll a\/b proteins of photosystem I in green alga Dunaliella salina","volume":"19","author":"Liang","year":"2008","journal-title":"DNA Seq."},{"key":"10.1016\/j.biortech.2026.135691_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.algal.2019.101697","article-title":"Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis","volume":"44","author":"Lin","year":"2019","journal-title":"Algal Res."},{"issue":"4","key":"10.1016\/j.biortech.2026.135691_b0120","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1111\/nph.20114","article-title":"Turning the industrially relevant marine alga Nannochloropsis red: one move for multifaceted benefits","volume":"244","author":"Liu","year":"2024","journal-title":"New Phytol."},{"issue":"4","key":"10.1016\/j.biortech.2026.135691_b0125","doi-asserted-by":"crossref","DOI":"10.1007\/s11103-024-01485-2","article-title":"The N-region sequence context impacts the chloroplast import efficiency of multi-TMD protein","volume":"114","author":"Nayak","year":"2024","journal-title":"Plant Mol. Biol."},{"issue":"3","key":"10.1016\/j.biortech.2026.135691_b0130","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1111\/1462-2920.13493","article-title":"Glycerol metabolism in hypersaline environments","volume":"19","author":"Oren","year":"2017","journal-title":"Environ. Microbiol."},{"issue":"10","key":"10.1016\/j.biortech.2026.135691_b0135","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1111\/pbi.13364","article-title":"Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii","volume":"18","author":"Perozeni","year":"2020","journal-title":"Plant Biotechnol. J."},{"issue":"9","key":"10.1016\/j.biortech.2026.135691_b0140","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1021\/acssynbio.5c00510","article-title":"Sustainable production of bio-based geraniol: Heterologous expression of early terpenoid pathway enzymes in Chlamydomonas reinhardtii","volume":"14","author":"Perozeni","year":"2025","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2026.135691_b0145","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00425-018-3050-3","article-title":"A hypothesis about the origin of carotenoid lipid droplets in the green algae Dunaliella and Haematococcus","volume":"249","author":"Pick","year":"2019","journal-title":"Planta"},{"key":"10.1016\/j.biortech.2026.135691_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.algal.2020.101990","article-title":"Genomic adaptations of the green alga Dunaliella salina to life under high salinity","volume":"50","author":"Polle","year":"2020","journal-title":"Algal Res."},{"key":"10.1016\/j.biortech.2026.135691_b0155","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2016.00185","article-title":"Redirecting the cyanobacterial bicarbonate transporters BicA and SbtA to the chloroplast envelope: Soluble and membrane cargos need different chloroplast targeting signals in plants","volume":"7","author":"Rolland","year":"2016","journal-title":"Front. Plant Sci."},{"issue":"3","key":"10.1016\/j.biortech.2026.135691_b0160","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1111\/pbi.70405","article-title":"Synthetic biology and metabolic engineering of microalgae for sustainable lipid and terpenoid production: an updated perspective","volume":"24","author":"Shitanaka","year":"2026","journal-title":"Plant Biotechnol. J."},{"key":"10.1016\/j.biortech.2026.135691_b0165","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.algal.2016.06.017","article-title":"Heterologous expression of \u03b2-carotene hydroxylase in Dunaliella salina by Agrobacterium-mediated genetic transformation","volume":"18","author":"Simon","year":"2016","journal-title":"Algal Res."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426017736?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426017736?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T14:23:49Z","timestamp":1793802229000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426017736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":33,"alternative-id":["S0960852426017736"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135691","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of chloroplast transit peptides inDunaliella salina and their application for heterologous astaxanthin production","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135691","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135691"}}