{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:11:41Z","timestamp":1790712701609,"version":"4.1.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072252"],"award-info":[{"award-number":["32072252"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFE0214900"],"award-info":[{"award-number":["2024YFE0214900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.biortech.2026.135923","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:27:41Z","timestamp":1790098061000},"page":"135923","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"PB","title":["A novel biorefinery approach for flaxseed oil and protein co-production using bio-based solvents"],"prefix":"10.1016","volume":"464","author":[{"given":"Ying","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7547-1542","authenticated-orcid":false,"given":"Yong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"13","key":"10.1016\/j.biortech.2026.135923_b0005","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1039\/C6GC00611F","article-title":"Updating and further expanding GSK's solvent sustainability guide","volume":"18","author":"Alder","year":"2016","journal-title":"Green Chem."},{"issue":"1","key":"10.1016\/j.biortech.2026.135923_b0010","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1186\/s40001-023-01203-6","article-title":"A comprehensive review of the health benefits of flaxseed oil in relation to its chemical composition and comparison with other omega-3-rich oils","volume":"28","author":"Al-Madhagy","year":"2023","journal-title":"Eur. J. Med. Res."},{"key":"10.1016\/j.biortech.2026.135923_b0015","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1039\/D6FB00012F","article-title":"Standardization of extraction process parameters and solvent reuse for sustainable extraction of omega-3-rich flaxseed oil","volume":"4","author":"Areekal","year":"2026","journal-title":"Sustain. Food Technol."},{"issue":"10","key":"10.1016\/j.biortech.2026.135923_b0020","doi-asserted-by":"crossref","first-page":"5138","DOI":"10.1002\/jsfa.12584","article-title":"Value-added processing of seeds from tomato sauce industry and comparison of extraction solvents on the chemical properties","volume":"103","author":"Askin","year":"2023","journal-title":"J. Sci. Food Agric."},{"issue":"22","key":"10.1016\/j.biortech.2026.135923_b0025","doi-asserted-by":"crossref","first-page":"8478","DOI":"10.1021\/acssuschemeng.4c01795","article-title":"Semi-industrial countercurrent extraction of soybean with 2-methyloxolane for high-quality protein and oil","volume":"12","author":"Bartier","year":"2024","journal-title":"ACS Sustain. Chem. Eng."},{"issue":"1","key":"10.1016\/j.biortech.2026.135923_b0030","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/pca.2864","article-title":"Green extraction of oil from Carum carvi seeds using bio-based solvent and supercritical fluid: evaluation of its antioxidant and anti-inflammatory activities","volume":"31","author":"Bourgou","year":"2020","journal-title":"Phytochem. Anal"},{"issue":"3","key":"10.1016\/j.biortech.2026.135923_b0035","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s41061-018-0191-6","article-title":"Green and bio-based solvents","volume":"376","author":"Calvo-Flores","year":"2018","journal-title":"Top. Curr. Chem."},{"issue":"24","key":"10.1016\/j.biortech.2026.135923_b0040","doi-asserted-by":"crossref","first-page":"4449","DOI":"10.3390\/foods12244449","article-title":"Structure characterization and functional properties of flaxseed protein-chlorogenic acid complex","volume":"12","author":"Cao","year":"2023","journal-title":"Foods"},{"key":"10.1016\/j.biortech.2026.135923_b0045","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1051\/ocl\/2024027","article-title":"Solvent solutions: comparing extraction methods for edible oils and proteins in a changing regulatory landscape. Part 1: Physical-properties","volume":"31","author":"Carr\u00e9","year":"2024","journal-title":"OCL"},{"issue":"14","key":"10.1016\/j.biortech.2026.135923_b0050","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1007\/s00216-017-0323-9","article-title":"A green analytical chemistry approach for lipid extraction: computation methods in the selection of green solvents as alternative to hexane","volume":"409","author":"Cascant","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"issue":"1\u20132","key":"10.1016\/j.biortech.2026.135923_b0055","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.foodchem.2003.07.038","article-title":"Isolation and structural characterization of the major protein fraction from NorMan flaxseed (Linum usitatissimum L.)","volume":"90","author":"Chung","year":"2005","journal-title":"Food Chem."},{"issue":"21","key":"10.1016\/j.biortech.2026.135923_b0060","doi-asserted-by":"crossref","first-page":"7211","DOI":"10.1021\/acssuschemeng.0c09252","article-title":"Dry and aqueous 2-methyloxolane as green solvents for simultaneous production of soybean oil and defatted meal","volume":"9","author":"Claux","year":"2021","journal-title":"ACS Sustain. Chem. Eng."},{"issue":"7","key":"10.1016\/j.biortech.2026.135923_b0065","doi-asserted-by":"crossref","DOI":"10.1111\/jfpe.13271","article-title":"Extraction of Brazil nut kernel oil using green solvents: Effects of the process variables in the oil yield and composition","volume":"42","author":"Cornelio-Santiago","year":"2019","journal-title":"J. Food Process Eng"},{"key":"10.1016\/j.biortech.2026.135923_b0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2025.120853","article-title":"Optimization and characterization of peach kernel oil extraction using supercritical fluid extraction","volume":"227","author":"Cui","year":"2025","journal-title":"Ind. Crop Prod."},{"key":"10.1016\/j.biortech.2026.135923_b0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2023.113653","article-title":"Lipidomics insight on differences in lipid profiles and phytosterol compositions of coconut oils extracted by classical and green solvents","volume":"174","author":"Cui","year":"2023","journal-title":"Food Res. Int."},{"issue":"22","key":"10.1016\/j.biortech.2026.135923_b0080","doi-asserted-by":"crossref","first-page":"10608","DOI":"10.1021\/jf9022383","article-title":"Effect of the solvent type and temperature on phytosterol contents and compositions of wheat straw, bran, and germ extracts","volume":"57","author":"Dunford","year":"2009","journal-title":"J. Agric. Food Chem."},{"issue":"10","key":"10.1016\/j.biortech.2026.135923_b0085","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.3390\/molecules23102444","article-title":"Bioprocessing of functional ingredients from flaxseed","volume":"23","author":"Dzuvor","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.biortech.2026.135923_b0090","first-page":"67","article-title":"Directive 2023\/175 amending Directive 2009\/32\/EC of the European Parliament and of the Council as regards 2-methyloxolane","volume":"L 25","author":"EU Commission","year":"2023","journal-title":"Off. J. Eur. Union"},{"issue":"15","key":"10.1016\/j.biortech.2026.135923_b0095","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1002\/jsfa.7056","article-title":"Fenugreek (Trigonella foenum graecum) seed protein isolate: extraction optimization, amino acid composition, thermo and functional properties","volume":"95","author":"Feyzi","year":"2015","journal-title":"J. Sci. Food Agric."},{"key":"10.1016\/j.biortech.2026.135923_b0100","first-page":"144","article-title":"Aqueous enzymatic extraction: a green, environmentally friendly and sustainable oil extraction technology","author":"Gao","year":"2024","journal-title":"Trends Food Sci Technol."},{"key":"10.1016\/j.biortech.2026.135923_b0105","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.jiec.2022.06.020","article-title":"Soybean oil extraction using ethyl acetate and 1-butanol: from solvent selection to thermodynamic assessment","volume":"113","author":"Gasparetto","year":"2022","journal-title":"J. Ind. Eng. Chem."},{"key":"10.1016\/j.biortech.2026.135923_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2025.107353","article-title":"Hydrophilic deep eutectic solvents endowed lipases excellent dynamic conformation and enhanced the efficiency of medium- and long-chain triglycerides synthesis","volume":"71","author":"Han","year":"2025","journal-title":"Food Biosci."},{"issue":"23","key":"10.1016\/j.biortech.2026.135923_b0115","doi-asserted-by":"crossref","first-page":"13325","DOI":"10.1080\/07391102.2023.2275178","article-title":"Lysozyme stability in various deep eutectic solvents using molecular dynamics simulations","volume":"42","author":"Hebbar","year":"2024","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.biortech.2026.135923_b0120","series-title":"2009. Oilseeds - Determination of oil content (Reference method)","author":"ISO 659","year":"2009"},{"key":"10.1016\/j.biortech.2026.135923_b0125","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.foodchem.2015.09.106","article-title":"Preparation, characterization and functional properties of flax seed protein isolate","volume":"197","author":"Kaushik","year":"2016","journal-title":"Food Chem."},{"key":"10.1016\/j.biortech.2026.135923_b0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodhyd.2020.105731","article-title":"Physicochemical properties and aroma profiles of flaxseed proteins extracted from whole flaxseed and flaxseed meal","volume":"104","author":"Lan","year":"2020","journal-title":"Food Hydrocoll."},{"issue":"14","key":"10.1016\/j.biortech.2026.135923_b0135","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1021\/acs.jafc.2c00976","article-title":"Activation spectra of human bitter taste receptors stimulated with cyclolinopeptides corresponding to fresh and aged Linseed oil","volume":"70","author":"Lang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2026.135923_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2024.125880","article-title":"Net hydrogen bond interaction driven supramolecular deep eutectic solvent formation: the case of cyclodextrins","volume":"412","author":"Li","year":"2024","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.biortech.2026.135923_b0145","article-title":"Deep eutectic solvents for ginsenoside extraction and inhibition of ginsenoside hydrolysis in Panax notoginseng leaves: methodology and mechanistic insights","volume":"513","author":"Liu","year":"2025","journal-title":"Chem. Eng. J."},{"issue":"12","key":"10.1016\/j.biortech.2026.135923_b0155","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0314421","article-title":"A Modified Green Star Area (MoGSA) and software to assess greenness of reactions in the chemistry laboratories","volume":"19","author":"Mansour","year":"2024","journal-title":"PLoS One"},{"key":"10.1016\/j.biortech.2026.135923_b0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2023.112986","article-title":"Effects of high-pressure homogenization on the physicochemical, foaming, and emulsifying properties of chickpea protein","volume":"170","author":"Ma","year":"2023","journal-title":"Food Res. Int."},{"key":"10.1016\/j.biortech.2026.135923_b0165","series-title":"Fundamentals of General, Organic, and Biological Chemistry","author":"McMurry","year":"2017"},{"issue":"4","key":"10.1016\/j.biortech.2026.135923_b0170","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1111\/1541-4337.12075","article-title":"Flaxseed-enriched cereal-based products: a review of the impact of processing conditions","volume":"13","author":"Mercier","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"10.1016\/j.biortech.2026.135923_b0175","first-page":"244","article-title":"Kinetics and thermodynamics of oil extraction from South African hass avocados using hexane as a solvent","volume":"37","author":"Mgoma","year":"2021","journal-title":"S. Afr. J. Chem. Eng."},{"issue":"9","key":"10.1016\/j.biortech.2026.135923_b0180","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.idairyj.2011.01.012","article-title":"On how \u03ba-casein affects the interactions between the heat-induced whey protein\/\u03ba-casein complexes and the casein micelles during the acid gelation of skim milk","volume":"21","author":"Morand","year":"2011","journal-title":"Int. Dairy J."},{"issue":"1","key":"10.1016\/j.biortech.2026.135923_b0185","doi-asserted-by":"crossref","DOI":"10.3390\/catal9010015","article-title":"An overview of recent research in the conversion of glycerol into biofuels, fuel additives and other bio-based chemicals","volume":"9","author":"Nda-Umar","year":"2019","journal-title":"Catalysts"},{"key":"10.1016\/j.biortech.2026.135923_b0190","article-title":"Cleaner production of iron-coated quartz sand composites for efficient phosphorus adsorption in sanitary wastewater: a design of experiments (DoE) approach","volume":"35","author":"Nunes","year":"2023","journal-title":"Sustain. Chem. Pharm."},{"issue":"15","key":"10.1016\/j.biortech.2026.135923_b0195","doi-asserted-by":"crossref","first-page":"3417","DOI":"10.3390\/molecules25153417","article-title":"2-Methyloxolane (2-MeOx) as sustainable lipophilic solvent to substitute hexane for green extraction of natural products. properties, applications, and perspectives","volume":"25","author":"Rapinel","year":"2020","journal-title":"Molecules"},{"issue":"2","key":"10.1016\/j.biortech.2026.135923_b0200","doi-asserted-by":"crossref","first-page":"264","DOI":"10.18006\/2023.11(2).264.279","article-title":"Positive impacts of integrating flaxseed meal as a potential feed supplement in livestock and poultry production: present scientific understanding","volume":"11","author":"Rajan","year":"2023","journal-title":"J. Exp. Biol. Agric. Sci."},{"issue":"8","key":"10.1016\/j.biortech.2026.135923_b0205","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3390\/antiox8080313","article-title":"Seed-roasting process affects oxidative stability of cold-pressed oils","volume":"8","author":"Rozanska","year":"2019","journal-title":"Antioxidants"},{"issue":"2","key":"10.1016\/j.biortech.2026.135923_b0210","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.jcis.2009.04.034","article-title":"Beta-lactoglobulin aggregates in foam films: correlation between foam films and foaming properties","volume":"336","author":"Rullier","year":"2009","journal-title":"J. Colloid Interf. Sci."},{"key":"10.1016\/j.biortech.2026.135923_b0220","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.trac.2019.05.046","article-title":"Hansen solubility parameters for selection of green extraction solvents","volume":"118","author":"Sanchez-Camargo","year":"2019","journal-title":"Trac-Trends Anal. Chem."},{"key":"10.1016\/j.biortech.2026.135923_b0225","doi-asserted-by":"crossref","DOI":"10.1063\/5.0251283","article-title":"Hydrogen-bond induced non-linear size dependence of lysozyme under the influence of aqueous glyceline","volume":"162","author":"Sarkar","year":"2025","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.biortech.2026.135923_b0230","doi-asserted-by":"crossref","first-page":"8430","DOI":"10.3390\/ijms16048430","article-title":"Alternative bio-based solvents for extraction of fat and oils: solubility prediction, global yield, extraction kinetics, chemical composition and cost of manufacturing","volume":"16","author":"Sicaire","year":"2015","journal-title":"Inter. J. Mol. Sci."},{"issue":"3","key":"10.1016\/j.biortech.2026.135923_b0235","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1080\/10408390903537241","article-title":"Flaxseed: a potential source of food, feed and fiber","volume":"51","author":"Singh","year":"2011","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.biortech.2026.135923_b0240","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.127074","article-title":"Exploration of deep eutectic solvent-based biphasic system for furfural production and enhancing enzymatic hydrolysis: Chemical, topochemical, and morphological changes","volume":"352","author":"Sun","year":"2022","journal-title":"Bioresour. Technol."},{"issue":"12","key":"10.1016\/j.biortech.2026.135923_b0245","doi-asserted-by":"crossref","first-page":"4988","DOI":"10.1111\/1750-3841.16787","article-title":"Effects of different extraction methods on volatile profiles of flaxseed oils","volume":"88","author":"Sun","year":"2023","journal-title":"J. Food Sci."},{"issue":"1","key":"10.1016\/j.biortech.2026.135923_b0250","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15586\/qas.v13i1.783","article-title":"Flaxseed oil: Extraction, Health benefits and products","volume":"13","author":"Tang","year":"2021","journal-title":"Qual. Assur. Saf. Crop. Foods"},{"issue":"7","key":"10.1016\/j.biortech.2026.135923_b0255","doi-asserted-by":"crossref","first-page":"8196","DOI":"10.3390\/molecules17078196","article-title":"Terpenes as green solvents for extraction of oil from microalgae","volume":"17","author":"Tanzi","year":"2012","journal-title":"Molecules"},{"key":"10.1016\/j.biortech.2026.135923_b0260","article-title":"% Greenness, a new metric to assess solvents","volume":"44","author":"Tovar","year":"2025","journal-title":"Sustain. Chem. Pharm."},{"key":"10.1016\/j.biortech.2026.135923_b0265","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2024.120313","article-title":"Investigation and assessment of equilibrium solubility, Hansen solubility parameters and modeling of natural plant medicine artemisinin (form I) in three binary solvents at different temperatures","volume":"224","author":"Zhang","year":"2025","journal-title":"Ind. Crop Prod."},{"key":"10.1016\/j.biortech.2026.135923_b0270","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.138148","article-title":"Effect of deep eutectic solvents on activity, stability, and selectivity of enzymes: Novel insights and further prospects","volume":"284","author":"Zhou","year":"2025","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426020055?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426020055?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:40:25Z","timestamp":1790710825000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426020055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":52,"alternative-id":["S0960852426020055"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135923","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel biorefinery approach for flaxseed oil and protein co-production using bio-based solvents","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.135923","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"135923"}}