{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:42:40Z","timestamp":1792762960716,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.biortech.2026.136092","type":"journal-article","created":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T21:51:51Z","timestamp":1792360311000},"page":"136092","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"PC","title":["Integrated genetic and process engineering of Pichia pastoris for high-yield ergothioneine production via methanol-free cultivation"],"prefix":"10.1016","volume":"464","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1363-877X","authenticated-orcid":false,"given":"Jia","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7613-501X","authenticated-orcid":false,"given":"Xin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3960-0989","authenticated-orcid":false,"given":"Chang-Hong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8016","key":"10.1016\/j.biortech.2026.136092_b0005","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.biortech.2026.136092_b0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.funbio.2026.101750","article-title":"Key genes for ergothioneine biosynthesis and stress adaptation in Schizophyllum commune","volume":"130","author":"Chen","year":"2026","journal-title":"Fungal Biol."},{"issue":"8","key":"10.1016\/j.biortech.2026.136092_b0015","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1002\/bit.70235","article-title":"High-level production of ergothioneine in Yarrowia lipolytica via systematic metabolic engineering strategies","volume":"123","author":"Chen","year":"2026","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.biortech.2026.136092_b0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2025.106745","article-title":"Enhancing ergothioneine production in Saccharomyces cerevisiae via protein fusion technology and gene integration optimization","volume":"68","author":"Chen","year":"2025","journal-title":"Food Biosci."},{"key":"10.1016\/j.biortech.2026.136092_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2025.106963","article-title":"Engineering Kluyveromyces lactis for de novo production of ergothioneine","volume":"69","author":"Chen","year":"2025","journal-title":"Food Biosci."},{"issue":"10","key":"10.1016\/j.biortech.2026.136092_b0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1002708","article-title":"CAVER 3.0: a tool for the analysis of transport pathways in dynamic protein structures","volume":"8","author":"Chovancov\u00e1","year":"2012","journal-title":"PLoS Comput. Biol."},{"issue":"1","key":"10.1016\/j.biortech.2026.136092_b0035","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12934-021-01648-6","article-title":"Screening and evaluation of the strong endogenous promoters in Pichia pastoris","volume":"20","author":"Dou","year":"2021","journal-title":"Microb. Cell Fact."},{"issue":"18","key":"10.1016\/j.biortech.2026.136092_b0040","doi-asserted-by":"crossref","first-page":"10832","DOI":"10.3390\/ijms231810832","article-title":"A single Aspergillus fumigatus gene enables ergothioneine biosynthesis and secretion by Saccharomyces cerevisiae","volume":"23","author":"Doyle","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"3","key":"10.1016\/j.biortech.2026.136092_b0045","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s10930-021-09968-7","article-title":"Production of industrial enzymes via Pichia pastoris as a cell factory in bioreactor: current status and future aspects","volume":"40","author":"Duman-\u00d6zdamar","year":"2021","journal-title":"Protein J."},{"issue":"1","key":"10.1016\/j.biortech.2026.136092_b0050","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s11274-023-03851-6","article-title":"Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system","volume":"40","author":"Eskandari","year":"2023","journal-title":"World J. Microbiol. Biotechnol."},{"issue":"14","key":"10.1016\/j.biortech.2026.136092_b0055","doi-asserted-by":"crossref","first-page":"8555","DOI":"10.1021\/acs.jafc.5c00598","article-title":"Engineering Pichia pastorisfor efficient de novo synthesis of 2'-fucosyllactose","volume":"73","author":"Fang","year":"2025","journal-title":"J. Agric. Food Chem."},{"issue":"2","key":"10.1016\/j.biortech.2026.136092_b0060","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41587-019-0363-0","article-title":"The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2","volume":"38","author":"Gassler","year":"2020","journal-title":"Nat. Biotechnol."},{"issue":"4","key":"10.1016\/j.biortech.2026.136092_b0065","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1021\/acsagscitech.4c00730","article-title":"Enhanced production of ergothioneine in Yarrowia lipolytica through combined metabolic and enzyme engineering","volume":"5","author":"Hu","year":"2025","journal-title":"ACS Agric. Sci. Technol."},{"issue":"20","key":"10.1016\/j.biortech.2026.136092_b0070","doi-asserted-by":"crossref","first-page":"5382","DOI":"10.1021\/ol502596z","article-title":"Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway","volume":"16","author":"Hu","year":"2014","journal-title":"Org. Lett."},{"issue":"4","key":"10.1016\/j.biortech.2026.136092_b0075","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s00253-019-10282-7","article-title":"High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris","volume":"104","author":"Huang","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"23","key":"10.1016\/j.biortech.2026.136092_b0080","doi-asserted-by":"crossref","first-page":"6390","DOI":"10.1021\/acs.jafc.0c01846","article-title":"High production of ergothioneine in Escherichia coli using the sulfoxide synthase from Methylobacterium strains","volume":"68","author":"Kamide","year":"2020","journal-title":"J. Agric. Food Chem."},{"issue":"15","key":"10.1016\/j.biortech.2026.136092_b0085","doi-asserted-by":"crossref","first-page":"9234","DOI":"10.1021\/acs.jafc.5c01267","article-title":"Enhancing ergothioneine production by combined protein and metabolic engineering strategies","volume":"73","author":"Kang","year":"2025","journal-title":"J. Agric. Food Chem."},{"issue":"6","key":"10.1016\/j.biortech.2026.136092_b0090","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/s11274-025-04415-6","article-title":"Research progress and development prospects of microbial synthesis of ergothioneine","volume":"41","author":"Lei","year":"2025","journal-title":"World J. Microbiol. Biotechnol."},{"issue":"11","key":"10.1016\/j.biortech.2026.136092_b0095","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1007\/s10529-013-1265-8","article-title":"Identification and characterization of PGCW14: a novel, strong constitutive promoter of Pichia pastoris","volume":"35","author":"Liang","year":"2013","journal-title":"Biotechnol. Lett"},{"issue":"24","key":"10.1016\/j.biortech.2026.136092_b0100","doi-asserted-by":"crossref","first-page":"15225","DOI":"10.1021\/acs.jafc.5c02834","article-title":"Integrating membrane permeability engineering and enzyme design for efficient production of ergothioneine in E. coli","volume":"73","author":"Ling","year":"2025","journal-title":"J. Agric. Food Chem."},{"issue":"5","key":"10.1016\/j.biortech.2026.136092_b0105","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.3390\/microorganisms14051088","article-title":"Engineering Escherichia coli for ergothioneine production via metabolic engineering and fermentation optimization","volume":"14","author":"Liu","year":"2026","journal-title":"Microorganisms"},{"key":"10.1016\/j.biortech.2026.136092_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2026.109498","article-title":"Application of the modular strategy to the production of ergothioneine by engineered Saccharomyces cerevisiae","volume":"83","author":"Ma","year":"2026","journal-title":"Food Biosci."},{"issue":"5","key":"10.1016\/j.biortech.2026.136092_b0115","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1021\/acs.jafc.7b04924","article-title":"Heterologous and high production of ergothioneine in Escherichia coli","volume":"66","author":"Osawa","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2026.136092_b0120","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"issue":"19","key":"10.1016\/j.biortech.2026.136092_b0125","doi-asserted-by":"crossref","first-page":"6632","DOI":"10.1021\/ja101721e","article-title":"In vitro reconstitution of mycobacterial ergothioneine biosynthesis","volume":"132","author":"Seebeck","year":"2010","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.biortech.2026.136092_b0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.crfs.2024.100840","article-title":"Optimal fermentation conditions for growth and recombinant protein production in Pichia pastoris: strain selection, ploidy level and carbon source","volume":"9","author":"Shemesh","year":"2024","journal-title":"Curr. Res. Food Sci."},{"issue":"2","key":"10.1016\/j.biortech.2026.136092_b0135","doi-asserted-by":"crossref","DOI":"10.1128\/aem.01740-23","article-title":"Identification of a novel growth-associated promoter for biphasic expression of heterogenous proteins in Pichia pastoris","volume":"90","author":"Shen","year":"2024","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2026.136092_b0140","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/978-1-0716-4330-3_16","article-title":"Heterologous expression of secreted proteins from Phytophthora in Pichia pastoris followed by protein purification by immobilized metal ion affinity (IMAC)","volume":"2892","author":"Tarallo","year":"2025","journal-title":"Methods Mol. Biol."},{"issue":"10","key":"10.1016\/j.biortech.2026.136092_b0145","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1002\/1873-3468.14239","article-title":"Engineering ergothioneine production in Yarrowia lipolytica","volume":"596","author":"Van der Hoek","year":"2022","journal-title":"FEBS Lett."},{"key":"10.1016\/j.biortech.2026.136092_b0150","doi-asserted-by":"crossref","unstructured":"Vogl, T., 2022. Engineering of promoters for gene expression in Pichia pastoris. In: Mapelli, V., Bettiga, M. (Eds.), Yeast Metabolic Engineering: Methods in Molecular Biology. Springer, New York, pp. 153-177.","DOI":"10.1007\/978-1-0716-2399-2_10"},{"issue":"11","key":"10.1016\/j.biortech.2026.136092_b0155","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1023\/A:1024034508136","article-title":"Application of a two-stage temperature control strategy for enhanced glutathione production in the batch fermentation by Candida utilis","volume":"25","author":"Wei","year":"2003","journal-title":"Biotechnol. Lett"},{"issue":"3","key":"10.1016\/j.biortech.2026.136092_b0160","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.3390\/cimb43030161","article-title":"Improved production of Streptomyces sp. FA1 xylanase in a dual-plasmid Pichia pastoris system","volume":"43","author":"Xia","year":"2021","journal-title":"Curr. Issues Mol. Biol."},{"issue":"2","key":"10.1016\/j.biortech.2026.136092_b0165","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.tibtech.2025.09.014","article-title":"Efficient ergothioneine production through reconstruction of the methyl and sulfur supply systems in Escherichia coli","volume":"44","author":"Yan","year":"2026","journal-title":"Trends Biotechnol."},{"issue":"36","key":"10.1016\/j.biortech.2026.136092_b0170","doi-asserted-by":"crossref","first-page":"13409","DOI":"10.1021\/acs.jafc.3c03709","article-title":"Combinatorial metabolic engineering of Escherichia coli for enhanced L-Cysteine production: insights into crucial regulatory modes and optimization of carbon-sulfur metabolism and cofactor availability","volume":"71","author":"Yang","year":"2023","journal-title":"J. Agric. Food Chem."},{"issue":"10","key":"10.1016\/j.biortech.2026.136092_b0175","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.3390\/jof9101027","article-title":"Advances in metabolic engineering of Pichia pastoris strains as powerful cell factories","volume":"9","author":"Zha","year":"2023","journal-title":"J. Fungi"},{"issue":"1","key":"10.1016\/j.biortech.2026.136092_b0180","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1021\/acs.jafc.2c07859","article-title":"Engineering methyltransferase and sulfoxide synthase for high-yield production of ergothioneine","volume":"71","author":"Zhang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.biortech.2026.136092_b0185","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jbiotec.2026.03.014","article-title":"Engineering Escherichia coli for high-level production of ergothioneine","volume":"414","author":"Zhang","year":"2026","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.biortech.2026.136092_b0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131446","article-title":"Low-carbon and overproduction of cordycepin from methanol using engineered Pichia pastoris cell factory","volume":"413","author":"Zhao","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"1","key":"10.1016\/j.biortech.2026.136092_b0195","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.carbpol.2013.06.060","article-title":"A new strategy to enhance gellan production by two-stage culture in Sphingomonas paucimobilis","volume":"98","author":"Zhu","year":"2013","journal-title":"Carbohydr. Polym."},{"issue":"9","key":"10.1016\/j.biortech.2026.136092_b0200","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3390\/fermentation8090431","article-title":"Ergothioneine production by submerged fermentation of a medicinal mushroom Panus conchatus","volume":"8","author":"Zhu","year":"2022","journal-title":"Fermentation"}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426021747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426021747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:30:44Z","timestamp":1792762244000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426021747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":40,"alternative-id":["S0960852426021747"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.136092","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated genetic and process engineering of Pichia pastoris for high-yield ergothioneine production via methanol-free cultivation","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.136092","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"136092"}}