{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:37:59Z","timestamp":1792730279598,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004263","name":"Foundation for Research Support of Rio Grande do Sul State","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Bioresource Technology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.biortech.2026.136106","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:50:28Z","timestamp":1792597828000},"page":"136106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"PC","title":["Optimizing initial inoculum ratios to enhance metabolite production and biocontrol activity in Bacillus subtilis and Pseudomonas fluorescens co-cultures"],"prefix":"10.1016","volume":"464","author":[{"given":"Let\u00edcia Welter","family":"Rother","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Luft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6626-2781","authenticated-orcid":false,"given":"Gustavo Andrade","family":"Ugalde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Lovato","family":"Colpo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio Antonio","family":"Mazutti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerson Vanderlei","family":"Carus Guedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas Andr\u00e9","family":"Arnemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.biortech.2026.136106_b0005","doi-asserted-by":"crossref","unstructured":"Abisado, R.G., Benomar, S., Klaus, J.R., Dandekar, A.A., Chandler, J.R., 2018. Bacterial quorum sensing and microbial community interactions. mBio. Doi: 10.1128\/mBio.02331-17.","DOI":"10.1128\/mBio.01749-18"},{"key":"10.1016\/j.biortech.2026.136106_b0010","series-title":"Identification of essential oil components by gas chromatography\/mass spectrometry","author":"Adams","year":"2012"},{"key":"10.1016\/j.biortech.2026.136106_b0015","doi-asserted-by":"crossref","DOI":"10.1128\/mr.43.2.260-296.1979","article-title":"Methanogens: Reevaluation of a Unique Biological Group","author":"Balch","year":"1979","journal-title":"Microbiol. Rev."},{"key":"10.1016\/j.biortech.2026.136106_b0020","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1094\/PHYTO-03-21-0128-R","article-title":"Biocontrol activity of Bacillus spp. and Pseudomonas spp. against botrytis cinerea and other cannabis fungal pathogens","volume":"112","author":"Balthazar","year":"2022","journal-title":"Phytopathology"},{"key":"10.1016\/j.biortech.2026.136106_b0025","series-title":"Illustrated genera of imperfect fungi","author":"Barnett","year":"2010"},{"key":"10.1016\/j.biortech.2026.136106_b0030","doi-asserted-by":"crossref","DOI":"10.3390\/fermentation11070410","article-title":"Microbial Genome Editing with CRISPR\u2013Cas9: Recent Advances and Emerging Applications Across Sectors","author":"Dudeja","year":"2025","journal-title":"Fermentation"},{"key":"10.1016\/j.biortech.2026.136106_b0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.164489","article-title":"Antibiotic removal by microalgae-bacteria consortium: Metabolic pathways and microbial responses","author":"Eheneden","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.biortech.2026.136106_b0040","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/s41396-020-00751-7","article-title":"The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity","volume":"15","author":"Gao","year":"2021","journal-title":"ISME J."},{"key":"10.1016\/j.biortech.2026.136106_b0045","doi-asserted-by":"crossref","DOI":"10.1038\/s43705-021-00049-5","article-title":"Emergent transcriptional adaption facilitates convergent succession within a synthetic community","volume":"1","author":"Gao","year":"2021","journal-title":"ISME Commun."},{"key":"10.1016\/j.biortech.2026.136106_b0050","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.biocontrol.2013.09.013","article-title":"Cell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mold by Trichoderma spp","volume":"67","author":"Geraldine","year":"2013","journal-title":"Biol. Control"},{"key":"10.1016\/j.biortech.2026.136106_b0055","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1615252","article-title":"Antifungal potential of Bacillus strains: implications for biocontrol strategies in food safety and sustainable agriculture","volume":"16","author":"Gharsallah","year":"2025","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0060","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1128\/AEM.02999-14","article-title":"Pseudomonas strains naturally associated with potato plants produce volatiles with high potential for inhibition of Phytophthora infestans","volume":"81","author":"Hunziker","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.tibtech.2023.05.008","article-title":"Construction of stable microbial consortia for effective biochemical synthesis","author":"Jiang","year":"2023","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.biortech.2026.136106_b0070","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-008-1760-3","article-title":"Bacterial volatiles and their action potential","author":"Kai","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.biortech.2026.136106_b0075","doi-asserted-by":"crossref","DOI":"10.1186\/s43014-024-00261-5","article-title":"Microbial enzymes and major applications in the food industry: a concise review","author":"Kumar","year":"2024","journal-title":"Food Prod. Process. Nutr."},{"key":"10.1016\/j.biortech.2026.136106_b0080","doi-asserted-by":"crossref","DOI":"10.1007\/s11033-025-10697-8","article-title":"Biocontrol potential of Pseudomonas fluorescens: phenazine mediated antifungal activity against phytopathogens","volume":"52","author":"Lakshmikanthan","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.biortech.2026.136106_b0085","series-title":"The Fusarium Laboratory Manual","author":"Leslie","year":"2006"},{"key":"10.1016\/j.biortech.2026.136106_b0090","doi-asserted-by":"crossref","unstructured":"L\u00e9toff\u00e9, S., Audrain, B., Bernier, S.P., Delepierre, M., Ghigo, J.M., 2014. Aerial exposure to the bacterial volatile compound trimethylamine modifies antibiotic resistance of physically separated bacteria by raising culture medium pH. mBio 5. Doi: 10.1128\/mBio.00944-13.","DOI":"10.1128\/mBio.00944-13"},{"key":"10.1016\/j.biortech.2026.136106_b0095","doi-asserted-by":"crossref","DOI":"10.1016\/j.apsoil.2023.105011","article-title":"Inoculated microbial consortia perform better than single strains in living soil: A meta-analysis","volume":"190","author":"Liu","year":"2023","journal-title":"Appl. Soil Ecol."},{"key":"10.1016\/j.biortech.2026.136106_b0100","doi-asserted-by":"crossref","unstructured":"Luft, L., Tonato, D., Valente, I. de L., Rother, L.W., Escobar, L.A. da S., Mazutti, M.A., 2026. Development of Wettable Powder Formulation of Bacillus subtilis and Its Biological Control Against Fungal Phytopathogens. Processes 14. Doi: 10.3390\/pr14121996.","DOI":"10.3390\/pr14121996"},{"key":"10.1016\/j.biortech.2026.136106_b0105","doi-asserted-by":"crossref","DOI":"10.3390\/agronomy14071535","article-title":"Consortium of phosphorus-solubilizing bacteria promotes maize growth and changes the microbial community composition of rhizosphere soil","volume":"14","author":"Luo","year":"2024","journal-title":"Agronomy"},{"key":"10.1016\/j.biortech.2026.136106_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.cpb.2019.100122","article-title":"Secondary metabolites synthesized by Stemphylium lycopersici and Fulvia fulva, necrotrophic and biotrophic fungi pathogen of tomato plants","volume":"20","author":"Medina","year":"2019","journal-title":"Curr. Plant Biol."},{"key":"10.1016\/j.biortech.2026.136106_b0115","unstructured":"Neilands, B.B., 1987. Universal Chemical Assay for the Detection and Determination of Siderophores\u2019, Analytical Biochemistry."},{"key":"10.1016\/j.biortech.2026.136106_b0120","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/ja.2017.51","article-title":"Novel antibiotic screening methods to awaken silent or cryptic secondary metabolic pathways in actinomycetes","volume":"70","author":"Onaka","year":"2017","journal-title":"J. Antibiot."},{"key":"10.1016\/j.biortech.2026.136106_b0125","doi-asserted-by":"crossref","DOI":"10.1038\/s41579-023-00980-5","article-title":"The interplay between microbial communities and soil properties","author":"Philippot","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0130","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2020106938","article-title":"1\u2010Undecene from Pseudomonas aeruginosa is an olfactory signal for flight\u2010or\u2010fight response in Caenorhabditis elegans","volume":"40","author":"Prakash","year":"2021","journal-title":"EMBO J."},{"key":"10.1016\/j.biortech.2026.136106_b0135","first-page":"523","article-title":"Volatile organic compounds produced by a soil-isolate, Bacillus subtilis FA26 induce adverse ultra-structural changes to the cells of Clavibacter michiganensis ssp. Sepedonicus, the causal agent of bacterial ring rot of potato","volume":"163","author":"Rajer","year":"2017","journal-title":"Microbiology (United Kingdom)"},{"key":"10.1016\/j.biortech.2026.136106_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.plaphy.2024.109092","article-title":"Antifungal mechanisms and characteristics of Pseudomonas fluorescens: Promoting peanut growth and combating Fusarium oxysporum-induced root rot","volume":"216","author":"Ren","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.biortech.2026.136106_b0145","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1286926","article-title":"An antibiotic produced by Pseudomonas fluorescens CFBP2392 with antifungal activity against Rhizoctonia solani","volume":"14","author":"Riera","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0150","unstructured":"Ryu, C.-M., Farag, M.A., Hu, C.-H., Reddy, M.S., Wei, H.-X., Par\u00e9, P.W., Kloepper, J.W., Jahn, D., 2003. Bacterial volatiles promote growth in Arabidopsis."},{"key":"10.1016\/j.biortech.2026.136106_b0155","doi-asserted-by":"crossref","unstructured":"Scherlach, K., Hertweck, C., 2021. Mining and unearthing hidden biosynthetic potential. Nat. Commun. Doi: 10.1038\/s41467-021-24133-5.","DOI":"10.1038\/s41467-021-24133-5"},{"key":"10.1016\/j.biortech.2026.136106_b0160","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1117559","article-title":"Enhancing chemical and biological diversity by co-cultivation","author":"Selegato","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0165","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1002\/mrc.4874","article-title":"Improvement of bioactive metabolite production in microbial cultures\u2014a systems approach by OSMAC and deconvolution-based 1HNMR quantification","volume":"57","author":"Selegato","year":"2019","journal-title":"Magn. Reson. Chem."},{"key":"10.1016\/j.biortech.2026.136106_b0170","doi-asserted-by":"crossref","unstructured":"Singh, G., & Arya, S. K. (2019). Antifungal and insecticidal potential of chitinases: A credible choice for the eco-friendly farming. In Biocatalysis and Agricultural Biotechnology (Vol. 20). Elsevier Ltd. Doi: 10.1016\/j.bcab.2019.101289.","DOI":"10.1016\/j.bcab.2019.101289"},{"key":"10.1016\/j.biortech.2026.136106_b0175","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11306-007-0082-2","article-title":"Proposed minimum reporting standards for chemical analysis","volume":"3","author":"Sumner","year":"2007","journal-title":"Metabolomics"},{"key":"10.1016\/j.biortech.2026.136106_b0180","doi-asserted-by":"crossref","DOI":"10.1007\/s00284-026-04921-2","article-title":"Optimization of culture media for trichoderma strains as a sustainable approach for agriculture","volume":"83","author":"Valente","year":"2026","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.biortech.2026.136106_b0185","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0021-9673(01)80947-X","article-title":"A generalization of the retention index system including linear temperature programmed gas\u2013liquid partition chromatography","volume":"11","author":"Van Den Dool","year":"1963","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.biortech.2026.136106_b0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcab.2025.103587","article-title":"Antifungal activity of GH64 \u03b2-1,3-glucanase from the gram-negative bacterium Flavobacterium johnsoniae","volume":"66","author":"Watanabe","year":"2025","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"10.1016\/j.biortech.2026.136106_b0195","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/mpp.12494","article-title":"Antibacterial effects of volatiles produced by bacillus strain D13 against xanthomonas oryzae pv. oryzae","volume":"19","author":"Xie","year":"2018","journal-title":"Mol. Plant Pathol"},{"key":"10.1016\/j.biortech.2026.136106_b0200","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/s41589-018-0193-2","article-title":"A genetics-free method for high-throughput discovery of cryptic microbial metabolites","volume":"15","author":"Xu","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.biortech.2026.136106_b0205","article-title":"Roles of microbial interactions in determining the establishment and function of synthetic consortium inoculants for soil applications","author":"Xu","year":"2026","journal-title":"ISME J."},{"key":"10.1016\/j.biortech.2026.136106_b0210","article-title":"Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens","volume":"10","author":"Yang","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.biortech.2026.136106_b0215","doi-asserted-by":"crossref","DOI":"10.1186\/s12866-022-02749-x","article-title":"Effect of volatile compounds produced by the cotton endophytic bacterial strain Bacillus sp. T6 against Verticillium wilt","volume":"23","author":"Zhang","year":"2023","journal-title":"BMC Microbiol."}],"container-title":["Bioresource Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426021887?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960852426021887?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:58:49Z","timestamp":1792727929000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960852426021887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":43,"alternative-id":["S0960852426021887"],"URL":"https:\/\/doi.org\/10.1016\/j.biortech.2026.136106","relation":{},"ISSN":["0960-8524"],"issn-type":[{"value":"0960-8524","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimizing initial inoculum ratios to enhance metabolite production and biocontrol activity in Bacillus subtilis and Pseudomonas fluorescens co-cultures","name":"articletitle","label":"Article Title"},{"value":"Bioresource Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.biortech.2026.136106","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"136106"}}